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Protein

Protein kinase rad3

Gene

rad3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which activates checkpoint signaling upon genotoxic stresses. Involved in G2 arrest following DNA damage where it phosphorylates chk1 (PubMed:8978690, PubMed:1398093). Phosphorylation of 'Thr-73' and 'Ser-80' of checkpoint mediator crb2 promotes its interaction with chk1 (PubMed:14739927). It is also involved in the dependence of mitosis on the completion of DNA replication (PubMed:1398093).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: CACAO
  • protein serine/threonine kinase activity Source: PomBase

GO - Biological processi

  • DNA biosynthetic process Source: UniProtKB-KW
  • DNA damage induced protein phosphorylation Source: PomBase
  • DNA repair Source: PomBase
  • histone H2A SQE motif phosphorylation Source: PomBase
  • intra-S DNA damage checkpoint Source: PomBase
  • mitotic DNA replication checkpoint Source: PomBase
  • mitotic G2 DNA damage checkpoint Source: PomBase
  • positive regulation of DNA repair Source: PomBase
  • positive regulation of mitotic DNA damage checkpoint Source: PomBase
  • telomere maintenance Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase rad3 (EC:2.7.11.1)
Alternative name(s):
DNA repair protein rad3
Gene namesi
Name:rad3
ORF Names:SPBC216.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC216.05.
PomBaseiSPBC216.05. rad3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear chromosome Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleolus Source: PomBase
  • protein complex Source: PomBase
  • subtelomeric heterochromatin Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23862386Protein kinase rad3PRO_0000088837Add
BLAST

Proteomic databases

MaxQBiQ02099.

PTM databases

iPTMnetiQ02099.

Interactioni

Subunit structurei

Interacts with crb2 (via BRCT domain). Interacts with chk1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
crb2P870743EBI-768555,EBI-768448

Protein-protein interaction databases

BioGridi277242. 114 interactions.
IntActiQ02099. 5 interactions.
MINTiMINT-4693394.

Structurei

3D structure databases

ProteinModelPortaliQ02099.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1386 – 1943558FATPROSITE-ProRule annotationAdd
BLAST
Domaini2018 – 2326309PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST
Domaini2354 – 238633FATCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family. ATM subfamily.Curated
Contains 1 FAT domain.PROSITE-ProRule annotation
Contains 1 FATC domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ02099.
KOiK06640.
OMAiFHLEQHA.
OrthoDBiEOG092C00SN.
PhylomeDBiQ02099.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
1.25.40.10. 3 hits.
InterProiIPR003152. FATC_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
IPR011990. TPR-like_helical_dom.
IPR012993. UME.
[Graphical view]
PfamiPF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF08064. UME. 1 hit.
[Graphical view]
SMARTiSM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
SM00802. UME. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQHAKRKAG SLDLSPRGLD DRQAFGQLLK EVLALDKEHE LGRSNSLPSM
60 70 80 90 100
TSELVEVLIE VGLLAFKHDD SKSEFISPKM LKEAHLSLQA LMLILKRSPT
110 120 130 140 150
VLREIKSSVT LLDWILPRTI SLFADIRFIK LFDSLKEFHK LIYQLISEKS
160 170 180 190 200
FLWDLYASFM RYWKYYITNV SSIVLQITNA TFPYKMPSPN SQPLQSITPN
210 220 230 240 250
YPTHREDKFD LLIINIEEAC TFFFESAHFF AQCSYLKKSN FPSPPLFTAW
260 270 280 290 300
TWIKPCFFNF VILLKRISIG DSQLFLHLHS RIVQTLCCFS LNFIYHGLPI
310 320 330 340 350
CEKSKHILMS SINLTLGSLK KTYTVANTAI SLFFLSLFVL PKTVAGLFYP
360 370 380 390 400
FGVSLLSDFK VLEQLEPDSD LKKAIILFKC RYQSSEIDQT TLRAFGEICT
410 420 430 440 450
GKLENTLFSN SELNLFLLHY LSLDNDLSNI LKVDFQNGHN ICTFAKWCIN
460 470 480 490 500
NNLDEPSNLK HFREMLDYYS SHNVTISEDD LKNFSLVLCT HVAKVNEKTN
510 520 530 540 550
SIFRTYEVHG CEVCNSFCLL FDERSLFKIP YHELFCALLK NPDIISSSVK
560 570 580 590 600
QSLLLDGFFR WSQHCSNFNK ESMLSLREFI MKALASTSRC LRVVAAKVLP
610 620 630 640 650
IFIKGPNNLD IVEFHKESKA LIFNTLKILA VENTAILETV ILSWISLSRV
660 670 680 690 700
VEEEELHFVL LEVISSVINS GIFYQGIGLS ALQQIASTRH ISVWQLLSPY
710 720 730 740 750
WPTVSVAIVQ GMGKKPNIAS LFAQLMNISE GDFLIRTQAY TLPFLVLTKN
760 770 780 790 800
KALIVRIAEL SQSDVATLCL TNMHKILASL LTTDHPNLEE SVMLLLSLAT
810 820 830 840 850
SDFEKVDLTS LLRSDPISIT VELLQLYQND VPHEKIENAL RKVAMIVSQV
860 870 880 890 900
VNDEDLSNKE LLYDFFNNHI LGILAEFSNI LNDLKGKTSI NEKIKTIVGI
910 920 930 940 950
EKMLSLCGGA VKLGLPQILS NLQSAFQNEH LRFYAIKAWF SLILATKEPE
960 970 980 990 1000
YSSIAGLSLV ILPPLFPYLE PQEAELVIQI FDFISSDTHK CLQGLKWAIP
1010 1020 1030 1040 1050
TSLDSACFSL KAKEIFCSLQ NEDFYSELQS IIKCLTNENE PVCYLGLQKL
1060 1070 1080 1090 1100
ELFFQAKVDE LHDTLNLDIS NEVLDQLLRC LLDCCVKYAS TNMQISYLAA
1110 1120 1130 1140 1150
KNLGELGAID PSRAKAQHII KETVVLDNFE NGEESLKFIL DFMQSQLIPA
1160 1170 1180 1190 1200
FLVTTDTKAQ GFLAYALQEF LKLGGFKSAV INKKKGLTVV TEHWMSLPDL
1210 1220 1230 1240 1250
SKRVLIPFLT SKYHLTPIPK IDIRYPIYKE NVTIHTWMQL FSLKLMEYAH
1260 1270 1280 1290 1300
SQNAEKIFGI CSKVVKDQEV NIPCFLLPFL VLNVILTESE LEVNKVIEEF
1310 1320 1330 1340 1350
QLVINQPGPD GLNSVGQQRY TSFVDVFFKI VDYLNKWLRM RKKRNWDRRS
1360 1370 1380 1390 1400
AIARKENRYM SVEDATSRES SISKVESFLS RFPSKTLGIV SLNCGFHARA
1410 1420 1430 1440 1450
LFYWEQHIRN ATAPYAALES DYRVLQEIYA GIDDPDEIEA VSLNFHDYSF
1460 1470 1480 1490 1500
DQQLLLHENS GTWDSALSCY EIIIQKDPEN KKAKIGLLNS MLQSGHYESL
1510 1520 1530 1540 1550
VLSLDSFIIN DNHEYSKMLN LGIEASWRSL SIDSLKKCLS KSNLESFEAK
1560 1570 1580 1590 1600
LGSIFYQYLR KDSFAELTER LQPLYVDAAT AIANTGAHSA YDCYDILSKL
1610 1620 1630 1640 1650
HAINDFSRIA ETDGIVSDNL DIVLRRRLSQ VAPYGKFKHQ ILSTHLVGYE
1660 1670 1680 1690 1700
KFENTKKTAE IYLEIARISR KNGQFQRAFN AILKAMDLDK PLATIEHAQW
1710 1720 1730 1740 1750
WWHQGQHRKA ISELNFSLNN NMFDLVDEHE ERPKNRKETL GNPLKGKVFL
1760 1770 1780 1790 1800
KLTKWLGKAG QLGLKDLETY YHKAVEIYSE CENTHYYLGH HRVLMYEEEQ
1810 1820 1830 1840 1850
KLPVNEQSER FLSGELVTRI INEFGRSLYY GTNHIYESMP KLLTLWLDFG
1860 1870 1880 1890 1900
AEELRLSKDD GEKYFREHII SSRKKSLELM NSNVCRLSMK IPQYFFLVAL
1910 1920 1930 1940 1950
SQMISRVCHP NNKVYKILEH IIANVVASYP GETLWQLMAT IKSTSQKRSL
1960 1970 1980 1990 2000
RGKSILNVLH SRKLSMSSKV DIKALSQSAI LITEKLINLC NTRINSKSVK
2010 2020 2030 2040 2050
MSLKDHFRLS FDDPVDLVIP AKSFLDITLP AKDANRASHY PFPKTQPTLL
2060 2070 2080 2090 2100
KFEDEVDIMN SLQKPRKVYV RGTDGNLYPF LCKPKDDLRK DARLMEFNNL
2110 2120 2130 2140 2150
ICKILRKDQE ANRRNLCIRT YVVIPLNEEC GFIEWVNHTR PFREILLKSY
2160 2170 2180 2190 2200
RQKNIPISYQ EIKVDLDFAL RSPNPGDIFE KKILPKFPPV FYEWFVESFP
2210 2220 2230 2240 2250
EPNNWVTSRQ NYCRTLAVMS IVGYVLGLGD RHGENILFDE FTGEAIHVDF
2260 2270 2280 2290 2300
NCLFDKGLTF EKPEKVPFRL THNMVDAMGP TGYEGGFRKA SEITMRLLRS
2310 2320 2330 2340 2350
NQDTLMSVLE SFLHDPLVEW NRKKSSSKYP NNEANEVLDI IRKKFQGFMP
2360 2370 2380
GETIPLSIEG QIQELIKSAV NPKNLVEMYI GWAAYF
Length:2,386
Mass (Da):273,529
Last modified:December 1, 2000 - v2
Checksum:iDDEA343FFA3872D3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981T → S in CAA70297 (PubMed:8978690).Curated
Sequence conflicti198 – 1981T → S in AAC49607 (PubMed:8978690).Curated
Sequence conflicti526 – 5261L → P in CAA70297 (PubMed:8978690).Curated
Sequence conflicti526 – 5261L → P in AAC49607 (PubMed:8978690).Curated
Sequence conflicti1779 – 17813SEC → LRM in CAA45106 (PubMed:1398093).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09076 Genomic DNA. Translation: CAA70297.1.
U76307 Genomic DNA. Translation: AAC49607.1.
CU329671 Genomic DNA. Translation: CAB40165.1.
X63544 Genomic DNA. Translation: CAA45106.1.
PIRiT39911.
RefSeqiNP_595357.1. NM_001021264.2.

Genome annotation databases

EnsemblFungiiSPBC216.05.1; SPBC216.05.1:pep; SPBC216.05.
GeneIDi2540719.
KEGGispo:SPBC216.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09076 Genomic DNA. Translation: CAA70297.1.
U76307 Genomic DNA. Translation: AAC49607.1.
CU329671 Genomic DNA. Translation: CAB40165.1.
X63544 Genomic DNA. Translation: CAA45106.1.
PIRiT39911.
RefSeqiNP_595357.1. NM_001021264.2.

3D structure databases

ProteinModelPortaliQ02099.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277242. 114 interactions.
IntActiQ02099. 5 interactions.
MINTiMINT-4693394.

PTM databases

iPTMnetiQ02099.

Proteomic databases

MaxQBiQ02099.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC216.05.1; SPBC216.05.1:pep; SPBC216.05.
GeneIDi2540719.
KEGGispo:SPBC216.05.

Organism-specific databases

EuPathDBiFungiDB:SPBC216.05.
PomBaseiSPBC216.05. rad3.

Phylogenomic databases

InParanoidiQ02099.
KOiK06640.
OMAiFHLEQHA.
OrthoDBiEOG092C00SN.
PhylomeDBiQ02099.

Miscellaneous databases

PROiQ02099.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
1.25.40.10. 3 hits.
InterProiIPR003152. FATC_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
IPR011990. TPR-like_helical_dom.
IPR012993. UME.
[Graphical view]
PfamiPF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF08064. UME. 1 hit.
[Graphical view]
SMARTiSM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
SM00802. UME. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAD3_SCHPO
AccessioniPrimary (citable) accession number: Q02099
Secondary accession number(s): Q92391, Q9UUM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.