Reviewed, UniProtKB/Swiss-Prot Q02096 (PGLR_PERAE)

Names and origin

Protein names	Recommended name:     Polygalacturonase       Short name=PG     EC=3.2.1.15 Alternative name(s):     Pectinase
Organism	Persea americana (Avocado)
Taxonomic identifier	3435 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › magnoliids › Laurales › Lauraceae › Persea

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in concert with the pectinesterase, in the ripening process. Is involved in cell wall metabolism, specifically in polyuronide degradation.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted. Secreted › cell wall.
Developmental stage	In ripening fruit.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Fruit ripening
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 462	440	Polygalacturonase		PRO_0000024811
Sites
	Active site	278	1	Proton donor By similarity
	Active site	301	1	By similarity
Amino acid modifications
	Glycosylation	294	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	182	1	S → I in AAA32914. Ref.2
	Sequence conflict	416 – 419	4	YIVG → ILLE in AAA32914. Ref.2
	Sequence conflict	442 – 462	21	LLREG…HECSY → TTEGKVYPPSCL in AAA32914. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q02096-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: ABCF068ACCB17A88	FASTA	462	50,291
	10 20 30 40 50 60 MALTRLLLPI SILWFCFYSS HTILQKDPLL ICVNGDPGFD QRAYPTYFGP ILDEFSSIMG 70 80 90 100 110 120 FEPSILSLER FNPVGGPETS PDTDISVDDF GARGDGTDDT KAFEKAWKDA CSSGSVLIVP 130 140 150 160 170 180 ENKNYLLKQI TFSGPCKSDL RVKIRGTIEA SSDQSDWVGH NRKRWIEFED ISNLTLEGGG 190 200 210 220 230 240 TSNGNGETWW DSSCKRKKSL PCKSAPTALT FRSCKNLIVS DLSIKDSQKM HLSFDKCQDV 250 260 270 280 290 300 IASNLMVTAP EHSPNTDGIH ITGTQRIHVM NSVIGTGDDC ISIESGSKMV IATNITCGPG 310 320 330 340 350 360 HGISIGSLGD RNSEAHVSGV LVDGGNLFDT TNGLRIKTWQ GGSGSAKNIK FQNIVMHNVT 370 380 390 400 410 420 NPIIIDQYYC DSKDPCPEQE SAVKVSNVAY MNIRGTSASE VAVKFDCSKS SPCQGYIVGN 430 440 450 460 INLVGNGGKE TTMSCSNIVQ GLLREGLSTF LFMKRRVHEC SY

References

[1]	"Cloning and characterization of avocado fruit mRNAs and their expression during ripening and low-temperature storage." Dopico B., Lowe A.L., Wilson I.D., Merodio C., Grierson D. Plant Mol. Biol. 21:437-449(1993) [PubMed: 8095163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Hass. Tissue: Pericarp.
[2]	"Ripening-related polygalacturonase cDNA from avocado." Kutsunai S.Y., Lin A.C., Percival F.W., Laties G.G., Christoffersen R.E. Plant Physiol. 103:289-290(1993) [PubMed: 8208850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Hass. Tissue: Mesocarp.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66426 mRNA. Translation: CAA47055.1. L06094 mRNA. Translation: AAA32914.1.
PIR	S31195.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 2559.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_PERAE

Accession

Primary (citable) accession number: Q02096

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)