Q02093 (DNLI_ACIAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:

lig

Organism

Acidianus ambivalens (Desulfurolobus ambivalens)

Taxonomic identifier

2283 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Acidianus

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	DNA ligase HAMAP MF_00407		PRO_0000059599
Sites
	Active site	261	1	N6-AMP-lysine intermediate By similarity
	Binding site	259	1	ATP By similarity
	Binding site	266	1	ATP By similarity
	Binding site	281	1	ATP By similarity
	Binding site	311	1	ATP By similarity
	Binding site	351	1	ATP By similarity
	Binding site	428	1	ATP By similarity
	Binding site	434	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02093 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 8FCBFF36F2475DD8	FASTA	600	67,618
	10 20 30 40 50 60 MEFKIIAEYF DRLEKISSRL QLTSLLADLF KKTDKNVIDK VVYIIQGKLW PDFLGMPELG 70 80 90 100 110 120 IGEKFLIRAL SIATSVSDDE IEKMYKSVGD LGQVAFDIKQ KQQSASILAF LGAQKASKPL 130 140 150 160 170 180 TVEKVYDDLA KVATSTGEGS RDIKIRLLAG LLKDASPLEA KYLVRFVDGR LRVGIGDATI 190 200 210 220 230 240 LDALAITFGG GQNFRPIVER AYNLRADLGN IAKILANGGI EQLKNIKPQP GIPIRPMLAE 250 260 270 280 290 300 RLSDPAEMLS KVGNIALVDY KYDGERGQIH KAGDKIFIFS RRLENITNQY PDVAEYISKY 310 320 330 340 350 360 VKGNEFIVEG EIIPVDPETG EMRPFQELMH RKRKSDIHEA IKEYPVNVFL FDLMYYEGED 370 380 390 400 410 420 YTVKPLSERR KKLESIVEDN DYVHIATHII TDNVEKLKEF FYQAISEGAE GVMVKSLAPD 430 440 450 460 470 480 AIYQAGSRGW LWIKFKRDYQ SEMADTVDLV MVGAFHGKGR KGGKYSSFLM AAYNPDKDVF 490 500 510 520 530 540 ETVCKVASGF TDAELDDLQK KIAELKRDTP HPRVVSTMVP DVWLTPALVA EIIGAEITIS 550 560 570 580 590 600 PLHTCCKDQY AEGGLSIRFP RFIRWRPDKS PEDATTNREI LEMYKSQLKK IEEKPSDQSV

References

[1]

"Molecular characterisation of a DNA ligase gene of the extremely thermophilic archaeon Desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases." Kletzin A. Nucleic Acids Res. 20:5389-5396(1992) [PubMed: 1437556] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Lei 10 / DSM 3772 / JCM 9191.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63438 Genomic DNA. Translation: CAA45034.1.
PIR	S26383.
3D structure databases
ProteinModelPortal	Q02093.
SMR	Q02093. Positions 1-590.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_ACIAM

Accession

Primary (citable) accession number: Q02093

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 31, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program