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Reviewed, UniProtKB/Swiss-Prot Q02093 (DNLI_ACIAM)

Last modified February 9, 2010. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
Gene names
Name: lig
OrganismAcidianus ambivalens (Desulfurolobus ambivalens)
Taxonomic identifier2283 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeAcidianus

Protein attributes

Sequence length600 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. HAMAP MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 600600DNA ligase HAMAP MF_00407
PRO_0000059599

Sites

Active site2611N6-AMP-lysine intermediate By similarity
Binding site2591ATP By similarity
Binding site2661ATP By similarity
Binding site2811ATP By similarity
Binding site3111ATP By similarity
Binding site3511ATP By similarity
Binding site4281ATP By similarity
Binding site4341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q02093-1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 8FCBFF36F2475DD8

FASTA60067,618
        10         20         30         40         50         60 
MEFKIIAEYF DRLEKISSRL QLTSLLADLF KKTDKNVIDK VVYIIQGKLW PDFLGMPELG 

        70         80         90        100        110        120 
IGEKFLIRAL SIATSVSDDE IEKMYKSVGD LGQVAFDIKQ KQQSASILAF LGAQKASKPL 

       130        140        150        160        170        180 
TVEKVYDDLA KVATSTGEGS RDIKIRLLAG LLKDASPLEA KYLVRFVDGR LRVGIGDATI 

       190        200        210        220        230        240 
LDALAITFGG GQNFRPIVER AYNLRADLGN IAKILANGGI EQLKNIKPQP GIPIRPMLAE 

       250        260        270        280        290        300 
RLSDPAEMLS KVGNIALVDY KYDGERGQIH KAGDKIFIFS RRLENITNQY PDVAEYISKY 

       310        320        330        340        350        360 
VKGNEFIVEG EIIPVDPETG EMRPFQELMH RKRKSDIHEA IKEYPVNVFL FDLMYYEGED 

       370        380        390        400        410        420 
YTVKPLSERR KKLESIVEDN DYVHIATHII TDNVEKLKEF FYQAISEGAE GVMVKSLAPD 

       430        440        450        460        470        480 
AIYQAGSRGW LWIKFKRDYQ SEMADTVDLV MVGAFHGKGR KGGKYSSFLM AAYNPDKDVF 

       490        500        510        520        530        540 
ETVCKVASGF TDAELDDLQK KIAELKRDTP HPRVVSTMVP DVWLTPALVA EIIGAEITIS 

       550        560        570        580        590        600 
PLHTCCKDQY AEGGLSIRFP RFIRWRPDKS PEDATTNREI LEMYKSQLKK IEEKPSDQSV 

« Hide

References

[1]"Molecular characterisation of a DNA ligase gene of the extremely thermophilic archaeon Desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases."
Kletzin A.
Nucleic Acids Res. 20:5389-5396(1992) [PubMed: 1437556] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Lei 10 / DSM 3772 / JCM 9191.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63438 Genomic DNA. Translation: CAA45034.1.
PIRS26383.

3D structure databases

SMRQ02093. Positions 1-590.
ModBaseSearch...

Enzyme and pathway databases

BRENDA6.5.1.1. 256365.

Family and domain databases

HAMAPMF_00407. DNA_ligase.
[Tree]
InterProIPR000977. DNA_ligase.
IPR012309. DNA_ligase_A_C.
IPR012310. DNA_ligase_A_M.
IPR016059. DNA_ligase_CS.
IPR012340. NA-bd_OB-fold.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
[Graphical view]
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_ACIAM
AccessionPrimary (citable) accession number: Q02093
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents