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Protein

Laccase-4

Gene

LCC4

Organism
Thanatephorus cucumeris (Black scurf of potato) (Rhizoctonia solani)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products.Curated

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

pH dependencei

Optimum pH is 7.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83Copper 1; type 2By similarity1
Metal bindingi85Copper 2; type 3By similarity1
Metal bindingi128Copper 2; type 3By similarity1
Metal bindingi130Copper 3; type 3By similarity1
Metal bindingi427Copper 4; type 1By similarity1
Metal bindingi430Copper 1; type 2By similarity1
Metal bindingi432Copper 3; type 3By similarity1
Metal bindingi479Copper 3; type 3By similarity1
Metal bindingi480Copper 4; type 1By similarity1
Metal bindingi481Copper 2; type 3By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Protein family/group databases

CAZyiAA1. Auxiliary Activities 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-4 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 4
Diphenol oxidase 4
Urishiol oxidase 4
Gene namesi
Name:LCC4
OrganismiThanatephorus cucumeris (Black scurf of potato) (Rhizoctonia solani)
Taxonomic identifieri107832 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesCantharellalesCeratobasidiaceaeThanatephorus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000000293820 – 531Laccase-4Add BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...)Sequence analysis1
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Glycosylationi186N-linked (GlcNAc...)Sequence analysis1
Glycosylationi231N-linked (GlcNAc...)Sequence analysis1
Glycosylationi280N-linked (GlcNAc...)Sequence analysis1
Glycosylationi395N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

In mycelia, at a higher level than LCC1, LCC2 and LCC3.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliQ02081.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 146Plastocyanin-like 1Add BLAST124
Domaini158 – 315Plastocyanin-like 2Add BLAST158
Domaini384 – 507Plastocyanin-like 3Add BLAST124

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSSITLLPL LAAVSTPAFA AVRNYKFDIK NVNVAPDGFQ RPIVSVNGLV
60 70 80 90 100
PGTLITANKG DTLRINVTNQ LTDPSMRRAT TIHWHGLFQA TTADEDGPAF
110 120 130 140 150
VTQCPIAQNL SYTYEIPLHG QTGTMWYHAH LASQYVDGLR GPLVIYDPND
160 170 180 190 200
PHKSRYDVDD ASTVVMLEDW YHTPAPVLEK QMFSTNNTAL LSPVPDSGLI
210 220 230 240 250
NGKGRYVGGP AVPRSVINVK RGKRYRLRVI NASAIGSFTF SIEGHRLTVI
260 270 280 290 300
EADGIPHQPL PVDSFQIYAG QRYSVIVEAN QTAANYWIRA PMTVAGAGTN
310 320 330 340 350
ANLDPTNVFA VLHYEGAPNA EPTTEQGSAI GTALVEENLH ALINPGAPGG
360 370 380 390 400
SAPADVSLNL AIGRSTVDGI LRFTFNNIKY EAPSLPTLLK ILANNASNDA
410 420 430 440 450
DFTPNEHTIV LPHNKVIGAQ HHRGADHPIH LHGHVFDIVK SLGGTPNYVN
460 470 480 490 500
PPRRDVVRVG GTGVVLRFKA DNPGPWFVHC HIDCTWRLGS HLSLPRPPAR
510 520 530
FARVSSRSSP TMPGTSSAPS TRLFLPICSK W
Length:531
Mass (Da):57,545
Last modified:November 1, 1996 - v1
Checksum:i737339803F75AB19
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti42P → S.1
Natural varianti119H → R.1
Natural varianti246R → S.1
Natural varianti256P → L.1
Natural varianti261P → A.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54277 Genomic DNA. Translation: CAA91042.1.
PIRiS68120.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54277 Genomic DNA. Translation: CAA91042.1.
PIRiS68120.

3D structure databases

ProteinModelPortaliQ02081.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiAA1. Auxiliary Activities 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiLAC4_THACU
AccessioniPrimary (citable) accession number: Q02081
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.