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Reviewed, UniProtKB/Swiss-Prot Q02081 (LAC4_THACU)

Last modified June 16, 2009. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Laccase-4
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 4
    Urishiol oxidase 4
    Diphenol oxidase 4
Gene names
Name: LCC4
OrganismThanatephorus cucumeris (Black scurf of potato) (Rhizoctonia solani)
Taxonomic identifier107832 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Cantharellales › Ceratobasidiaceae › Thanatephorus

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Protein attributes

Sequence length531 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Lignin degradation and detoxification of lignin-derived products Probable.

Catalytic activity

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactor

Binds 4 copper ions per monomer By similarity.

Subunit structure

Homodimer.

Subcellular location

Secreted.

Tissue specificity

In mycelia, at a higher level than LCC1, LCC2 and LCC3.

Sequence similarities

Belongs to the multicopper oxidase family.

Contains 3 plastocyanin-like domains.

biophysicochemical properties

pH dependence:

Optimum pH is 7.

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Ontologies

Keywords
   Biological processLignin degradation
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainRepeat
Signal
   LigandCopper
Metal-binding
   Molecular functionOxidoreductase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processlignin catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncopper ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

laccase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919
Chain20 – 531512Laccase-4
PRO_0000002938

Regions

Domain23 – 146124Plastocyanin-like 1
Domain158 – 315158Plastocyanin-like 2
Domain384 – 507124Plastocyanin-like 3

Sites

Metal binding831Copper 1; type 2 By similarity
Metal binding851Copper 2; type 3 By similarity
Metal binding1281Copper 2; type 3 By similarity
Metal binding1301Copper 3; type 3 By similarity
Metal binding4271Copper 4; type 1 By similarity
Metal binding4301Copper 1; type 2 By similarity
Metal binding4321Copper 3; type 3 By similarity
Metal binding4791Copper 3; type 3 By similarity
Metal binding4801Copper 4; type 1 By similarity
Metal binding4811Copper 2; type 3 By similarity

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation1091N-linked (GlcNAc...) Potential
Glycosylation1861N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation2801N-linked (GlcNAc...) Potential
Glycosylation3951N-linked (GlcNAc...) Potential

Natural variations

Natural variant421P → S
Natural variant1191H → R
Natural variant2461R → S
Natural variant2561P → L
Natural variant2611P → A

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Sequences

Sequence LengthMass (Da)Tools
Q02081-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 737339803F75AB19

FASTA53157,545
        10         20         30         40         50         60 
MLSSITLLPL LAAVSTPAFA AVRNYKFDIK NVNVAPDGFQ RPIVSVNGLV PGTLITANKG 

        70         80         90        100        110        120 
DTLRINVTNQ LTDPSMRRAT TIHWHGLFQA TTADEDGPAF VTQCPIAQNL SYTYEIPLHG 

       130        140        150        160        170        180 
QTGTMWYHAH LASQYVDGLR GPLVIYDPND PHKSRYDVDD ASTVVMLEDW YHTPAPVLEK 

       190        200        210        220        230        240 
QMFSTNNTAL LSPVPDSGLI NGKGRYVGGP AVPRSVINVK RGKRYRLRVI NASAIGSFTF 

       250        260        270        280        290        300 
SIEGHRLTVI EADGIPHQPL PVDSFQIYAG QRYSVIVEAN QTAANYWIRA PMTVAGAGTN 

       310        320        330        340        350        360 
ANLDPTNVFA VLHYEGAPNA EPTTEQGSAI GTALVEENLH ALINPGAPGG SAPADVSLNL 

       370        380        390        400        410        420 
AIGRSTVDGI LRFTFNNIKY EAPSLPTLLK ILANNASNDA DFTPNEHTIV LPHNKVIGAQ 

       430        440        450        460        470        480 
HHRGADHPIH LHGHVFDIVK SLGGTPNYVN PPRRDVVRVG GTGVVLRFKA DNPGPWFVHC 

       490        500        510        520        530 
HIDCTWRLGS HLSLPRPPAR FARVSSRSSP TMPGTSSAPS TRLFLPICSK W

« Hide

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References

[1]"The identification and characterization of four laccases from the plant pathogenic fungus Rhizoctonia solani."
Wahleithner J.A., Xu F., Brown K.M., Brown S.H., Golightly E.J., Halkier T., Kauppinen S., Pederson A., Schneider P.
Curr. Genet. 29:395-403(1996) [PubMed: 8598061] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: RS22.

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Cross-references

Sequence databases

Z54277 Genomic DNA. Translation: CAA91042.1.
PIRS68120.

3D structure databases

HSSPHSSP built from PDB template 1HFU based on UniProtKB Q9Y780.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.10.3.2. 81600.

Family and domain databases

InterProIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 3 hits.
PfamPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
PROSITEPS00079. MULTICOPPER_OXIDASE1. False negative.
PS00080. MULTICOPPER_OXIDASE2. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLAC4_THACU
AccessionPrimary (citable) accession number: Q02081
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents