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Protein

Myocyte-specific enhancer factor 2B

Gene

MEF2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi58 – 8629Mef2-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. histone deacetylase binding Source: BHF-UCL
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  4. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. muscle organ development Source: ProtInc
  2. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_21402. CDO in myogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Myocyte-specific enhancer factor 2B
Alternative name(s):
RSRFR2
Serum response factor-like protein 2
Gene namesi
Name:MEF2B
Synonyms:XMEF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:6995. MEF2B.

Subcellular locationi

GO - Cellular componenti

  1. cell junction Source: HPA
  2. cytoplasm Source: HPA
  3. nucleoplasm Source: HPA
  4. nucleus Source: HPA
  5. transcription factor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Myocyte-specific enhancer factor 2BPRO_0000199431Add
BLAST

Proteomic databases

MaxQBiQ02080.
PaxDbiQ02080.
PRIDEiQ02080.

PTM databases

PhosphoSiteiQ02080.

Expressioni

Tissue specificityi

Expressed in skeletal and cardiac muscle and brain.

Gene expression databases

BgeeiQ02080.
CleanExiHS_MEF2B.
ExpressionAtlasiQ02080. baseline.
GenevestigatoriQ02080.

Organism-specific databases

HPAiHPA004734.

Interactioni

Subunit structurei

Interacts with HDAC7 (By similarity). Heterodimer. Interacts with HDAC9.By similarity1 Publication

Protein-protein interaction databases

BioGridi110371. 4 interactions.
IntActiQ02080. 1 interaction.
STRINGi9606.ENSP00000393159.

Structurei

Secondary structure

1
365
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 3926Combined sources
Beta strandi42 – 487Combined sources
Beta strandi54 – 607Combined sources
Helixi62 – 709Combined sources
Beta strandi76 – 805Combined sources
Helixi81 – 9313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N6JX-ray2.20A/B2-94[»]
1TQEX-ray2.70P/Q/R/S1-93[»]
ProteinModelPortaliQ02080.
SMRiQ02080. Positions 2-94.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02080.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5755MADS-boxPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 3128Lys-rich (basic)Add
BLAST

Sequence similaritiesi

Belongs to the MEF2 family.Curated
Contains 1 MADS-box domain.PROSITE-ProRule annotation
Contains 1 Mef2-type DNA-binding domain.Curated

Phylogenomic databases

eggNOGiCOG5068.
GeneTreeiENSGT00390000011828.
HOGENOMiHOG000230620.
HOVERGENiHBG053944.
InParanoidiQ02080.
KOiK09261.
PhylomeDBiQ02080.
TreeFamiTF314067.

Family and domain databases

InterProiIPR002100. TF_MADSbox.
[Graphical view]
PfamiPF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q02080-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRKKIQISR ILDQRNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS
60 70 80 90 100
ANRLFQYAST DMDRVLLKYT EYSEPHESRT NTDILETLKR RGIGLDGPEL
110 120 130 140 150
EPDEGPEEPG EKFRRLAGEG GDPALPRPRL YPAAPAMPSP DVVYGALPPP
160 170 180 190 200
GCDPSGLGEA LPAQSRPSPF RPAAPKAGPP GLVHPLFSPS HLTSKTPPPL
210 220 230 240 250
YLPTEGRRSD LPGGLAGPRG GLNTSRSLYS GLQNPCSTAT PGPPLGSFPF
260 270 280 290 300
LPGGPPVGAE AWARRVPQPA APPRRPPQSA SSLSASLRPP GAPATFLRPS
310 320 330 340 350
PIPCSSPGPW QSLCGLGPPC AGCPWPTAGP GRRSPGGTSP ERSPGTARAR
360
GDPTSLQASS EKTQQ
Length:365
Mass (Da):38,639
Last modified:January 31, 1996 - v2
Checksum:iB12A19B1F659C7C7
GO
Isoform 2 (identifier: Q02080-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     257-365: VGAEAWARRV...LQASSEKTQQ → EYGLGDPPPP...RLPLADGWPR

Show »
Length:368
Mass (Da):39,144
Checksum:i239CC949587FB544
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951K → R in BAG62689 (PubMed:14702039).Curated
Sequence conflicti195 – 1951K → R in BAH14699 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei257 – 365109VGAEA…EKTQQ → EYGLGDPPPPPGLLQPPTLA PWQPSRGDGPPAVSSQPSGG RSLGEEGPPTRGASPPTPPV SIKSERLSPAPGGPGDFPKT FPYPLLLARSLAEPLRPGPA LRRLPLADGWPR in isoform 2. 1 PublicationVSP_042322Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68502 mRNA. Translation: CAA48515.1.
X63380 mRNA. Translation: CAA44978.1.
AK301086 mRNA. Translation: BAG62689.1.
AK316328 mRNA. Translation: BAH14699.1.
AC002126 Genomic DNA. Translation: AAB86982.1.
CH471106 Genomic DNA. Translation: EAW84788.1.
BC126245 mRNA. Translation: AAI26246.1.
BC136457 mRNA. Translation: AAI36458.1.
BC171767 mRNA. Translation: AAI71767.1.
CCDSiCCDS46024.1. [Q02080-2]
PIRiA39481.
RefSeqiNP_001139257.1. NM_001145785.1. [Q02080-2]
NP_005910.1. NM_005919.3. [Q02080-1]
UniGeneiHs.153629.

Genome annotation databases

EnsembliENST00000424583; ENSP00000402154; ENSG00000213999. [Q02080-2]
GeneIDi100271849.
4207.
KEGGihsa:100271849.
hsa:4207.
UCSCiuc002nll.2. human. [Q02080-2]
uc002nlo.2. human. [Q02080-1]

Polymorphism databases

DMDMi1346514.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68502 mRNA. Translation: CAA48515.1.
X63380 mRNA. Translation: CAA44978.1.
AK301086 mRNA. Translation: BAG62689.1.
AK316328 mRNA. Translation: BAH14699.1.
AC002126 Genomic DNA. Translation: AAB86982.1.
CH471106 Genomic DNA. Translation: EAW84788.1.
BC126245 mRNA. Translation: AAI26246.1.
BC136457 mRNA. Translation: AAI36458.1.
BC171767 mRNA. Translation: AAI71767.1.
CCDSiCCDS46024.1. [Q02080-2]
PIRiA39481.
RefSeqiNP_001139257.1. NM_001145785.1. [Q02080-2]
NP_005910.1. NM_005919.3. [Q02080-1]
UniGeneiHs.153629.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N6JX-ray2.20A/B2-94[»]
1TQEX-ray2.70P/Q/R/S1-93[»]
ProteinModelPortaliQ02080.
SMRiQ02080. Positions 2-94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110371. 4 interactions.
IntActiQ02080. 1 interaction.
STRINGi9606.ENSP00000393159.

PTM databases

PhosphoSiteiQ02080.

Polymorphism databases

DMDMi1346514.

Proteomic databases

MaxQBiQ02080.
PaxDbiQ02080.
PRIDEiQ02080.

Protocols and materials databases

DNASUi4207.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000424583; ENSP00000402154; ENSG00000213999. [Q02080-2]
GeneIDi100271849.
4207.
KEGGihsa:100271849.
hsa:4207.
UCSCiuc002nll.2. human. [Q02080-2]
uc002nlo.2. human. [Q02080-1]

Organism-specific databases

CTDi100271849.
4207.
GeneCardsiGC19M019256.
HGNCiHGNC:6995. MEF2B.
HPAiHPA004734.
MIMi600661. gene.
neXtProtiNX_Q02080.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5068.
GeneTreeiENSGT00390000011828.
HOGENOMiHOG000230620.
HOVERGENiHBG053944.
InParanoidiQ02080.
KOiK09261.
PhylomeDBiQ02080.
TreeFamiTF314067.

Enzyme and pathway databases

ReactomeiREACT_21402. CDO in myogenesis.

Miscellaneous databases

EvolutionaryTraceiQ02080.
GeneWikiiMEF2B.
NextBioi16574.
PROiQ02080.
SOURCEiSearch...

Gene expression databases

BgeeiQ02080.
CleanExiHS_MEF2B.
ExpressionAtlasiQ02080. baseline.
GenevestigatoriQ02080.

Family and domain databases

InterProiIPR002100. TF_MADSbox.
[Graphical view]
PfamiPF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors."
    Yu Y.-T., Breitbart R.E., Smoot L.B., Lee Y., Mahdavi V., Nadal-Ginard B.
    Genes Dev. 6:1783-1798(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Heart and Skeletal muscle.
  2. "Human SRF-related proteins: DNA-binding properties and potential regulatory targets."
    Pollock R., Treisman R.
    Genes Dev. 5:2327-2341(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Spleen.
  4. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain and Testis.
  7. "Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte enhancer factor-2."
    Han A., Pan F., Stroud J.C., Youn H.-D., Liu J.O., Chen L.
    Nature 422:730-734(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 2-94.
  8. "Mechanism of recruitment of class II histone deacetylases by myocyte enhancer factor-2."
    Han A., He J., Wu Y., Liu J.O., Chen L.
    J. Mol. Biol. 345:91-102(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-93 IN COMPLEX WITH HDAC9 AND DNA.

Entry informationi

Entry nameiMEF2B_HUMAN
AccessioniPrimary (citable) accession number: Q02080
Secondary accession number(s): A0AV80
, B4DVH7, B7ZVY1, G5E9M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: January 31, 1996
Last modified: February 3, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.