Q02080 (MEF2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myocyte-specific enhancer factor 2B

Alternative name(s):
RSRFR2
Serum response factor-like protein 2

Gene names

Name:	MEF2B
Synonyms:	XMEF2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.
Subunit structure	Interacts with HDAC7 By similarity. Heterodimer. Interacts with HDAC9.
Subcellular location	Nucleus.
Tissue specificity	Expressed in skeletal and cardiac muscle and brain.
Sequence similarities	Belongs to the MEF2 family. Contains 1 MADS-box domain. Contains 1 Mef2-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	muscle organ development Traceable author statement Ref.1. Source: ProtInc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcription factor complex Inferred from direct assay Ref.8. Source: BHF-UCL
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Non-traceable author statement PubMed 8575763. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q02080-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q02080-2)

The sequence of this isoform differs from the canonical sequence as follows:
     257-365: VGAEAWARRV...LQASSEKTQQ → EYGLGDPPPP...RLPLADGWPR

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 365

365

Myocyte-specific enhancer factor 2B

PRO_0000199431

Regions

Domain

3 – 57

55

MADS-box

DNA binding

58 – 86

29

Mef2-type Potential

Compositional bias

4 – 31

28

Lys-rich (basic)

Natural variations

Alternative sequence

257 – 365

109

VGAEA…EKTQQ → EYGLGDPPPPPGLLQPPTLA PWQPSRGDGPPAVSSQPSGG RSLGEEGPPTRGASPPTPPV SIKSERLSPAPGGPGDFPKT FPYPLLLARSLAEPLRPGPA LRRLPLADGWPR in isoform 2.

VSP_042322

Experimental info

Sequence conflict

195

1

K → R in BAG62689. Ref.3

Sequence conflict

195

1

K → R in BAH14699. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

365

Helix Strand Turn

Details...

Helix

14 – 39

26

Beta strand

42 – 48

7

Beta strand

54 – 60

7

Helix

62 – 70

9

Beta strand

76 – 80

5

Helix

81 – 93

13

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: B12A19B1F659C7C7	FASTA	365	38,639
	10 20 30 40 50 60 MGRKKIQISR ILDQRNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS ANRLFQYAST 70 80 90 100 110 120 DMDRVLLKYT EYSEPHESRT NTDILETLKR RGIGLDGPEL EPDEGPEEPG EKFRRLAGEG 130 140 150 160 170 180 GDPALPRPRL YPAAPAMPSP DVVYGALPPP GCDPSGLGEA LPAQSRPSPF RPAAPKAGPP 190 200 210 220 230 240 GLVHPLFSPS HLTSKTPPPL YLPTEGRRSD LPGGLAGPRG GLNTSRSLYS GLQNPCSTAT 250 260 270 280 290 300 PGPPLGSFPF LPGGPPVGAE AWARRVPQPA APPRRPPQSA SSLSASLRPP GAPATFLRPS 310 320 330 340 350 360 PIPCSSPGPW QSLCGLGPPC AGCPWPTAGP GRRSPGGTSP ERSPGTARAR GDPTSLQASS EKTQQ
	Isoform 2 [UniParc]. Checksum: 239CC949587FB544	FASTA	368	39,144
	10 20 30 40 50 60 MGRKKIQISR ILDQRNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS ANRLFQYAST 70 80 90 100 110 120 DMDRVLLKYT EYSEPHESRT NTDILETLKR RGIGLDGPEL EPDEGPEEPG EKFRRLAGEG 130 140 150 160 170 180 GDPALPRPRL YPAAPAMPSP DVVYGALPPP GCDPSGLGEA LPAQSRPSPF RPAAPKAGPP 190 200 210 220 230 240 GLVHPLFSPS HLTSKTPPPL YLPTEGRRSD LPGGLAGPRG GLNTSRSLYS GLQNPCSTAT 250 260 270 280 290 300 PGPPLGSFPF LPGGPPEYGL GDPPPPPGLL QPPTLAPWQP SRGDGPPAVS SQPSGGRSLG 310 320 330 340 350 360 EEGPPTRGAS PPTPPVSIKS ERLSPAPGGP GDFPKTFPYP LLLARSLAEP LRPGPALRRL PLADGWPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors." Yu Y.-T., Breitbart R.E., Smoot L.B., Lee Y., Mahdavi V., Nadal-Ginard B. Genes Dev. 6:1783-1798(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Heart and Skeletal muscle.
[2]	"Human SRF-related proteins: DNA-binding properties and potential regulatory targets." Pollock R., Treisman R. Genes Dev. 5:2327-2341(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Spleen.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Testis.
[7]	"Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte enhancer factor-2." Han A., Pan F., Stroud J.C., Youn H.-D., Liu J.O., Chen L. Nature 422:730-734(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 2-94.
[8]	"Mechanism of recruitment of class II histone deacetylases by myocyte enhancer factor-2." Han A., He J., Wu Y., Liu J.O., Chen L. J. Mol. Biol. 345:91-102(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-93 IN COMPLEX WITH HDAC9 AND DNA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X68502 mRNA. Translation: CAA48515.1.
X63380 mRNA. Translation: CAA44978.1.
AK301086 mRNA. Translation: BAG62689.1.
AK316328 mRNA. Translation: BAH14699.1.
AC002126 Genomic DNA. Translation: AAB86982.1.
CH471106 Genomic DNA. Translation: EAW84788.1.
BC126245 mRNA. Translation: AAI26246.1.
BC136457 mRNA. Translation: AAI36458.1.
BC171767 mRNA. Translation: AAI71767.1.

IPI

IPI00024082.

PIR

A39481.

RefSeq

NP_001139257.1. NM_001145785.1.
NP_005910.1. NM_005919.3.

UniGene

Hs.153629.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1N6J	X-ray	2.20	A/B	2-94	[»]
1TQE	X-ray	2.70	P/Q/R/S	1-93	[»]

ProteinModelPortal

Q02080.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q02080. 1 interaction.

STRING

9606.ENSP00000393159.

PTM databases

PhosphoSite

Q02080.

Proteomic databases

PaxDb

Q02080.

PRIDE

Q02080.

Protocols and materials databases

DNASU

4207.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000162023; ENSP00000162023; ENSG00000213999.
ENST00000424583; ENSP00000402154; ENSG00000213999.
ENST00000444486; ENSP00000390762; ENSG00000213999.

GeneID

100271849.
4207.

KEGG

hsa:100271849.
hsa:4207.

UCSC

uc002nlo.2. human.

Organism-specific databases

CTD

100271849.
4207.

GeneCards

GC19M019256.

HGNC

HGNC:6995. MEF2B.

HPA

HPA004734.

MIM

600661. gene.

neXtProt

NX_Q02080.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5068.

HOGENOM

HOG000230620.

HOVERGEN

HBG053944.

KO

K09261.

PhylomeDB

Q02080.

Enzyme and pathway databases

Reactome

REACT_111045. Developmental Biology.

Gene expression databases

ArrayExpress

Q02080.

Bgee

Q02080.

CleanEx

HS_MEF2B.

Genevestigator

Q02080.

GermOnline

ENSG00000064489. Homo sapiens.

Family and domain databases

InterPro

IPR002100. TF_MADSbox.
[Graphical view]

Pfam

PF00319. SRF-TF. 1 hit.
[Graphical view]

PRINTS

PR00404. MADSDOMAIN.

SMART

SM00432. MADS. 1 hit.
[Graphical view]

SUPFAM

SSF55455. TF_MADSbox. 1 hit.

PROSITE

PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q02080.

NextBio

16574.

SOURCE

Search...

Entry information

Entry name

MEF2B_HUMAN

Accession

Primary (citable) accession number: Q02080
Secondary accession number(s): A0AV80 , B4DVH7, B7ZVY1, G5E9M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.