ID ASCL1_MOUSE Reviewed; 231 AA. AC Q02067; Q7TNT5; DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot. DT 03-OCT-2012, sequence version 2. DT 27-MAR-2024, entry version 192. DE RecName: Full=Achaete-scute homolog 1 {ECO:0000303|PubMed:8217843, ECO:0000303|PubMed:8424959}; DE Short=ASH-1 {ECO:0000303|PubMed:8217843, ECO:0000303|PubMed:8424959}; DE Short=mASH-1 {ECO:0000303|PubMed:8217843, ECO:0000303|PubMed:8424959}; DE Short=mASH1 {ECO:0000303|PubMed:8217843, ECO:0000303|PubMed:8424959}; GN Name=Ascl1 {ECO:0000312|MGI:MGI:96919}; GN Synonyms=Ash1 {ECO:0000303|PubMed:8217843, GN ECO:0000303|PubMed:8424959}, Mash-1 {ECO:0000303|PubMed:8217843, GN ECO:0000303|PubMed:8424959}, Mash1 {ECO:0000303|PubMed:8217843, GN ECO:0000303|PubMed:8424959}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE. RX PubMed=8424959; DOI=10.1016/0167-4781(93)90076-p; RA del Amo F., Gendron-Maguire M., Gridley T.; RT "Cloning, sequencing and expression of the mouse mammalian achaete-scute RT homolog 1 (MASH1)."; RL Biochim. Biophys. Acta 1171:323-327(1993). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL RP STAGE. RX PubMed=8217843; DOI=10.1016/0925-4773(93)90006-j; RA Guillemot F., Joyner A.L.; RT "Dynamic expression of the murine Achaete-Scute homologue Mash-1 in the RT developing nervous system."; RL Mech. Dev. 42:171-185(1993). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=C57BL/6J; TISSUE=Eye; RX PubMed=16141072; DOI=10.1126/science.1112014; RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.; RT "The transcriptional landscape of the mammalian genome."; RL Science 309:1559-1563(2005). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=C57BL/6J; TISSUE=Brain; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [7] RP SUBCELLULAR LOCATION. RX PubMed=1576967; DOI=10.1242/dev.114.1.75; RA Johnson J.E., Zimmerman K., Saito T., Anderson D.J.; RT "Induction and repression of mammalian achaete-scute homologue (MASH) gene RT expression during neuronal differentiation of P19 embryonal carcinoma RT cells."; RL Development 114:75-87(1992). RN [8] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=8221886; DOI=10.1016/0092-8674(93)90381-y; RA Guillemot F., Lo L.C., Johnson J.E., Auerbach A., Anderson D.J., RA Joyner A.L.; RT "Mammalian achaete-scute homolog 1 is required for the early development of RT olfactory and autonomic neurons."; RL Cell 75:463-476(1993). RN [9] RP FUNCTION IN NEUROGENESIS, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY. RX PubMed=16020526; DOI=10.1073/pnas.0504799102; RA Li S., Misra K., Matise M.P., Xiang M.; RT "Foxn4 acts synergistically with Mash1 to specify subtype identity of V2 RT interneurons in the spinal cord."; RL Proc. Natl. Acad. Sci. U.S.A. 102:10688-10693(2005). RN [10] RP FUNCTION IN NEUROGENESIS. RX PubMed=17728344; DOI=10.1242/dev.005868; RA Del Barrio M.G., Taveira-Marques R., Muroyama Y., Yuk D.I., Li S., RA Wines-Samuelson M., Shen J., Smith H.K., Xiang M., Rowitch D., RA Richardson W.D.; RT "A regulatory network involving Foxn4, Mash1 and delta-like 4/Notch1 RT generates V2a and V2b spinal interneurons from a common progenitor pool."; RL Development 134:3427-3436(2007). RN [11] RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION RP PHENOTYPE. RX PubMed=18173746; DOI=10.1111/j.1365-2443.2007.01146.x; RA Kokubu H., Ohtsuka T., Kageyama R.; RT "Mash1 is required for neuroendocrine cell development in the glandular RT stomach."; RL Genes Cells 13:41-51(2008). RN [12] RP FUNCTION. RX PubMed=20107439; DOI=10.1038/nature08797; RA Vierbuchen T., Ostermeier A., Pang Z.P., Kokubu Y., Suedhof T.C., RA Wernig M.; RT "Direct conversion of fibroblasts to functional neurons by defined RT factors."; RL Nature 463:1035-1041(2010). RN [13] RP FUNCTION, AND DNA-BINDING. RX PubMed=24243019; DOI=10.1016/j.cell.2013.09.028; RA Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., RA Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., RA Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., RA Wernig M.; RT "Hierarchical mechanisms for direct reprogramming of fibroblasts to RT neurons."; RL Cell 155:621-635(2013). RN [14] RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-151, AND IDENTIFICATION BY MASS RP SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Liver; RX PubMed=23576753; DOI=10.1073/pnas.1302961110; RA Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., RA Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.; RT "Label-free quantitative proteomics of the lysine acetylome in mitochondria RT identifies substrates of SIRT3 in metabolic pathways."; RL Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013). RN [15] RP FUNCTION. RX PubMed=27281220; DOI=10.1038/nature18323; RA Treutlein B., Lee Q.Y., Camp J.G., Mall M., Koh W., Shariati S.A., Sim S., RA Neff N.F., Skotheim J.M., Wernig M., Quake S.R.; RT "Dissecting direct reprogramming from fibroblast to neuron using single- RT cell RNA-seq."; RL Nature 534:391-395(2016). CC -!- FUNCTION: Transcription factor that plays a key role in neuronal CC differentiation: acts as a pioneer transcription factor, accessing CC closed chromatin to allow other factors to bind and activate neural CC pathways (PubMed:24243019). Directly binds the E box motif (5'-CANNTG- CC 3') on promoters and promotes transcription of neuronal genes CC (PubMed:20107439, PubMed:24243019, PubMed:27281220). The combination of CC three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is CC sufficient to reprogram fibroblasts and other somatic cells into CC induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, CC PubMed:27281220). Plays a role at early stages of development of CC specific neural lineages in most regions of the CNS, and of several CC lineages in the PNS (PubMed:8217843). Essential for the generation of CC olfactory and autonomic neurons (PubMed:8221886). Acts synergistically CC with FOXN4 to specify the identity of V2b neurons rather than V2a from CC bipotential p2 progenitors during spinal cord neurogenesis, probably CC through DLL4-NOTCH signaling activation (PubMed:16020526, CC PubMed:17728344). Involved in the regulation of neuroendocrine cell CC development in the glandular stomach (PubMed:18173746). CC {ECO:0000269|PubMed:16020526, ECO:0000269|PubMed:17728344, CC ECO:0000269|PubMed:18173746, ECO:0000269|PubMed:20107439, CC ECO:0000269|PubMed:24243019, ECO:0000269|PubMed:27281220, CC ECO:0000269|PubMed:8217843, ECO:0000269|PubMed:8221886}. CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH CC protein. Forms a heterodimer with TCF3. {ECO:0000250|UniProtKB:P50553}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:1576967}. CC -!- TISSUE SPECIFICITY: Developing CNS and PNS at embryonic and postnatal CC stages. Expressed in the epithelium of glandular stomach CC (PubMed:18173746). {ECO:0000269|PubMed:16020526, CC ECO:0000269|PubMed:18173746, ECO:0000269|PubMed:8217843, CC ECO:0000269|PubMed:8424959}. CC -!- DEVELOPMENTAL STAGE: Between 8.5 dpc and 10.5 dpc it is found in the CC neuroepithelium of the midbrain and ventral forebrain, as well as in CC the spinal cord. Between 10.5 dpc and 12.5 dpc its expression pattern CC changes from a restricted to a widespread zone, it is then found at CC variable levels in the ventricular zone in all regions of the brain, CC where is expressed in a subset of p2 progenitors that can give rise to CC either V2a or V2b interneuron subtypes. From 12.5 dpc to postnatal CC stages it is also expressed in cells outside of the ventricular zone CC through the brain, and in addition it is also expressed during CC development of the olfactory epithelium and neural retina. At 12.5 dpc, CC it is highly expressed by differentiating enteric neurons in the CC mesenchyme of the stomach. At 14.5 and 16.5 dpc, it is also expressed CC in the epithelium of the glandular stomach (PubMed:18173746). CC {ECO:0000269|PubMed:16020526, ECO:0000269|PubMed:18173746, CC ECO:0000269|PubMed:8217843, ECO:0000269|PubMed:8424959}. CC -!- DISRUPTION PHENOTYPE: Lethality at birth caused by severe defects in CC neurogenesis. While the brain and spinal cord of the mutants appear CC normal, their olfactory epithelium and sympathetic, parasympathetic and CC enteric ganglia are severely affected. In the olfactory epithelium, CC neuronal progenitors die at an early stage, whereas the non-neuronal CC supporting cells are present. In sympathetic ganglia, the development CC of neuronal precursors is arrested, preventing the generation of CC sympathetic neurons, without affecting glial precursor cells. CC Homozygous MASH1-null mice have smaller stomachs than the control, and CC neuroendocrine cells are mostly missing, while chief, parietal and pit CC cells are formed. However, the wall of the glandular stomach is much CC thicker, has a deeper fold structure, and the forestomach epithelium is CC villous compared to controls (PubMed:18173746). CC {ECO:0000269|PubMed:18173746, ECO:0000269|PubMed:8221886}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M95603; AAA37780.1; -; mRNA. DR EMBL; AK143210; BAE25307.1; -; mRNA. DR EMBL; AC122360; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH466539; EDL21455.1; -; Genomic_DNA. DR EMBL; BC055748; AAH55748.1; -; mRNA. DR CCDS; CCDS24101.1; -. DR PIR; S28186; S28186. DR RefSeq; NP_032579.2; NM_008553.4. DR AlphaFoldDB; Q02067; -. DR SMR; Q02067; -. DR BioGRID; 201314; 9. DR STRING; 10090.ENSMUSP00000020243; -. DR iPTMnet; Q02067; -. DR PhosphoSitePlus; Q02067; -. DR PaxDb; 10090-ENSMUSP00000020243; -. DR ProteomicsDB; 281917; -. DR Antibodypedia; 18050; 601 antibodies from 40 providers. DR DNASU; 17172; -. DR Ensembl; ENSMUST00000020243.10; ENSMUSP00000020243.8; ENSMUSG00000020052.10. DR GeneID; 17172; -. DR KEGG; mmu:17172; -. DR UCSC; uc007gqs.1; mouse. DR AGR; MGI:96919; -. DR CTD; 429; -. DR MGI; MGI:96919; Ascl1. DR VEuPathDB; HostDB:ENSMUSG00000020052; -. DR eggNOG; KOG4029; Eukaryota. DR GeneTree; ENSGT00940000162483; -. DR HOGENOM; CLU_063523_3_0_1; -. DR InParanoid; Q02067; -. DR OMA; QPAACFF; -. DR OrthoDB; 3086567at2759; -. DR PhylomeDB; Q02067; -. DR TreeFam; TF322889; -. DR BioGRID-ORCS; 17172; 3 hits in 78 CRISPR screens. DR ChiTaRS; Ascl1; mouse. DR PRO; PR:Q02067; -. DR Proteomes; UP000000589; Chromosome 10. DR RNAct; Q02067; Protein. DR Bgee; ENSMUSG00000020052; Expressed in medial ganglionic eminence and 125 other cell types or tissues. DR GO; GO:0043025; C:neuronal cell body; IDA:MGI. DR GO; GO:0005634; C:nucleus; IDA:MGI. DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central. DR GO; GO:0043425; F:bHLH transcription factor binding; ISO:MGI. DR GO; GO:0003682; F:chromatin binding; IDA:MGI. DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI. DR GO; GO:0003690; F:double-stranded DNA binding; ISO:MGI. DR GO; GO:0070888; F:E-box binding; ISO:MGI. DR GO; GO:0042802; F:identical protein binding; ISO:MGI. DR GO; GO:0003676; F:nucleic acid binding; ISO:MGI. DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro. DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI. DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central. DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI. DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI. DR GO; GO:0061104; P:adrenal chromaffin cell differentiation; IMP:MGI. DR GO; GO:0061103; P:carotid body glomus cell differentiation; IMP:MGI. DR GO; GO:0048469; P:cell maturation; IMP:MGI. DR GO; GO:0071259; P:cellular response to magnetism; IEA:Ensembl. DR GO; GO:0021954; P:central nervous system neuron development; IMP:MGI. DR GO; GO:0021987; P:cerebral cortex development; IMP:MGI. DR GO; GO:0021892; P:cerebral cortex GABAergic interneuron differentiation; IEA:Ensembl. DR GO; GO:0021902; P:commitment of neuronal cell to specific neuron type in forebrain; IMP:MGI. DR GO; GO:0048484; P:enteric nervous system development; TAS:BHF-UCL. DR GO; GO:0021879; P:forebrain neuron differentiation; ISO:MGI. DR GO; GO:0097154; P:GABAergic neuron differentiation; IGI:MGI. DR GO; GO:0048699; P:generation of neurons; IMP:MGI. DR GO; GO:0007507; P:heart development; IEA:Ensembl. DR GO; GO:0061100; P:lung neuroendocrine cell differentiation; IMP:MGI. DR GO; GO:0097475; P:motor neuron migration; IMP:MGI. DR GO; GO:0050883; P:musculoskeletal movement, spinal reflex action; IMP:MGI. DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI. DR GO; GO:0045892; P:negative regulation of DNA-templated transcription; ISO:MGI. DR GO; GO:0045665; P:negative regulation of neuron differentiation; ISO:MGI. DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI. DR GO; GO:0007399; P:nervous system development; IDA:UniProtKB. DR GO; GO:0061351; P:neural precursor cell proliferation; IMP:MGI. DR GO; GO:0007400; P:neuroblast fate determination; IMP:MGI. DR GO; GO:0007405; P:neuroblast proliferation; IGI:MGI. DR GO; GO:0061101; P:neuroendocrine cell differentiation; IMP:MGI. DR GO; GO:0022008; P:neurogenesis; ISO:MGI. DR GO; GO:0048666; P:neuron development; IDA:UniProtKB. DR GO; GO:0030182; P:neuron differentiation; IDA:UniProtKB. DR GO; GO:0048663; P:neuron fate commitment; IDA:UniProtKB. DR GO; GO:0048665; P:neuron fate specification; IDA:UniProtKB. DR GO; GO:0003358; P:noradrenergic neuron development; IMP:UniProtKB. DR GO; GO:0003359; P:noradrenergic neuron fate commitment; ISO:MGI. DR GO; GO:0007219; P:Notch signaling pathway; IMP:MGI. DR GO; GO:0060166; P:olfactory pit development; IMP:MGI. DR GO; GO:0021779; P:oligodendrocyte cell fate commitment; IMP:MGI. DR GO; GO:0014003; P:oligodendrocyte development; IMP:MGI. DR GO; GO:0048709; P:oligodendrocyte differentiation; IMP:MGI. DR GO; GO:0048486; P:parasympathetic nervous system development; TAS:BHF-UCL. DR GO; GO:0007389; P:pattern specification process; IMP:MGI. DR GO; GO:0048935; P:peripheral nervous system neuron development; IMP:MGI. DR GO; GO:0045787; P:positive regulation of cell cycle; IDA:MGI. DR GO; GO:2000179; P:positive regulation of neural precursor cell proliferation; IMP:MGI. DR GO; GO:0050769; P:positive regulation of neurogenesis; IMP:MGI. DR GO; GO:0043525; P:positive regulation of neuron apoptotic process; IMP:MGI. DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISS:UniProtKB. DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; IMP:MGI. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI. DR GO; GO:0042127; P:regulation of cell population proliferation; IDA:MGI. DR GO; GO:0030856; P:regulation of epithelial cell differentiation; IMP:MGI. DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB. DR GO; GO:0007346; P:regulation of mitotic cell cycle; IMP:MGI. DR GO; GO:0050767; P:regulation of neurogenesis; IMP:MGI. DR GO; GO:0008593; P:regulation of Notch signaling pathway; IMP:MGI. DR GO; GO:0060165; P:regulation of timing of subpallium neuron differentiation; IMP:MGI. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB. DR GO; GO:0070849; P:response to epidermal growth factor; IEA:Ensembl. DR GO; GO:0051593; P:response to folic acid; IEA:Ensembl. DR GO; GO:0032526; P:response to retinoic acid; IEA:Ensembl. DR GO; GO:0007423; P:sensory organ development; IBA:GO_Central. DR GO; GO:0021527; P:spinal cord association neuron differentiation; IMP:MGI. DR GO; GO:0021529; P:spinal cord oligodendrocyte cell differentiation; IGI:MGI. DR GO; GO:0021530; P:spinal cord oligodendrocyte cell fate specification; IMP:MGI. DR GO; GO:0061102; P:stomach neuroendocrine cell differentiation; IMP:MGI. DR GO; GO:0060163; P:subpallium neuron fate commitment; IMP:MGI. DR GO; GO:0061549; P:sympathetic ganglion development; IMP:UniProtKB. DR GO; GO:0048485; P:sympathetic nervous system development; TAS:BHF-UCL. DR GO; GO:0060579; P:ventral spinal cord interneuron fate commitment; IMP:UniProtKB. DR GO; GO:0021750; P:vestibular nucleus development; IMP:MGI. DR CDD; cd19742; bHLH_TS_ASCL1_Mash1; 1. DR Gene3D; 4.10.280.10; Helix-loop-helix DNA-binding domain; 1. DR InterPro; IPR011598; bHLH_dom. DR InterPro; IPR036638; HLH_DNA-bd_sf. DR PANTHER; PTHR23349; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST; 1. DR PANTHER; PTHR23349:SF57; FACTOR IN THE GERMLINE ALPHA; 1. DR Pfam; PF00010; HLH; 1. DR SMART; SM00353; HLH; 1. DR SUPFAM; SSF47459; HLH, helix-loop-helix DNA-binding domain; 1. DR PROSITE; PS50888; BHLH; 1. DR Genevisible; Q02067; MM. PE 1: Evidence at protein level; KW Acetylation; Activator; Developmental protein; Differentiation; KW DNA-binding; Neurogenesis; Nucleus; Reference proteome; Transcription; KW Transcription regulation. FT CHAIN 1..231 FT /note="Achaete-scute homolog 1" FT /id="PRO_0000127127" FT DOMAIN 113..165 FT /note="bHLH" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981" FT REGION 1..24 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 39..92 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 1..15 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 42..69 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 151 FT /note="N6-acetyllysine" FT /evidence="ECO:0007744|PubMed:23576753" FT CONFLICT 81 FT /note="V -> D (in Ref. 1; AAA37780)" FT /evidence="ECO:0000305" FT CONFLICT 160 FT /note="E -> Q (in Ref. 1; AAA37780)" FT /evidence="ECO:0000305" SQ SEQUENCE 231 AA; 24741 MW; 454E6937520BD4CA CRC64; MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAQSAQQQQ PQAPPQQAPQ LSPVADSQPS GGGHKSAAKQ VKRQRSSSPE LMRCKRRLNF SGFGYSLPQQ QPAAVARRNE RERNRVKLVN LGFATLREHV PNGAANKKMS KVETLRSAVE YIRALQQLLD EHDAVSAAFQ AGVLSPTISP NYSNDLNSMA GSPVSSYSSD EGSYDPLSPE EQELLDFTNW F //