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Protein

Achaete-scute homolog 1

Gene

Ascl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5'-CANNTG-3'). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons.4 Publications

GO - Molecular functioni

  1. bHLH transcription factor binding Source: MGI
  2. chromatin binding Source: MGI
  3. DNA binding Source: UniProtKB
  4. double-stranded DNA binding Source: Ensembl
  5. E-box binding Source: MGI
  6. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: MGI
  7. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: MGI
  8. sequence-specific DNA binding Source: MGI
  9. sequence-specific DNA binding transcription factor activity Source: MGI
  10. transcription factor binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. adrenal chromaffin cell differentiation Source: MGI
  2. carotid body glomus cell differentiation Source: MGI
  3. cell differentiation Source: MGI
  4. cell maturation Source: MGI
  5. cellular response to magnetism Source: Ensembl
  6. central nervous system neuron development Source: MGI
  7. cerebral cortex development Source: MGI
  8. cerebral cortex GABAergic interneuron differentiation Source: Ensembl
  9. commitment of neuronal cell to specific neuron type in forebrain Source: MGI
  10. enteric nervous system development Source: BHF-UCL
  11. generation of neurons Source: MGI
  12. glial cell differentiation Source: MGI
  13. lung neuroendocrine cell differentiation Source: MGI
  14. musculoskeletal movement, spinal reflex action Source: MGI
  15. negative regulation of apoptotic process Source: MGI
  16. negative regulation of neuron differentiation Source: MGI
  17. negative regulation of transcription, DNA-templated Source: MGI
  18. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  19. neuroblast fate determination Source: MGI
  20. neuroblast proliferation Source: MGI
  21. neurogenesis Source: MGI
  22. neuron development Source: MGI
  23. neuron differentiation Source: MGI
  24. neuron fate commitment Source: MGI
  25. neuron fate specification Source: MGI
  26. neuron migration Source: MGI
  27. noradrenergic neuron development Source: UniProtKB
  28. noradrenergic neuron fate commitment Source: MGI
  29. Notch signaling pathway Source: MGI
  30. olfactory pit development Source: MGI
  31. oligodendrocyte cell fate commitment Source: MGI
  32. oligodendrocyte development Source: MGI
  33. oligodendrocyte differentiation Source: MGI
  34. parasympathetic nervous system development Source: BHF-UCL
  35. pattern specification process Source: MGI
  36. positive regulation of cell cycle Source: MGI
  37. positive regulation of neural precursor cell proliferation Source: MGI
  38. positive regulation of neurogenesis Source: MGI
  39. positive regulation of neuron apoptotic process Source: MGI
  40. positive regulation of neuron differentiation Source: UniProtKB
  41. positive regulation of Notch signaling pathway Source: MGI
  42. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  43. regulation of cell proliferation Source: MGI
  44. regulation of epithelial cell differentiation Source: MGI
  45. regulation of gene expression Source: UniProtKB
  46. regulation of mitotic cell cycle Source: MGI
  47. regulation of neurogenesis Source: MGI
  48. regulation of Notch signaling pathway Source: MGI
  49. regulation of timing of subpallium neuron differentiation Source: MGI
  50. regulation of transcription from RNA polymerase II promoter Source: MGI
  51. response to epidermal growth factor Source: Ensembl
  52. response to folic acid Source: Ensembl
  53. response to lithium ion Source: Ensembl
  54. response to retinoic acid Source: Ensembl
  55. spinal cord association neuron differentiation Source: MGI
  56. spinal cord oligodendrocyte cell differentiation Source: MGI
  57. spinal cord oligodendrocyte cell fate specification Source: MGI
  58. stomach neuroendocrine cell differentiation Source: MGI
  59. subpallium neuron fate commitment Source: MGI
  60. sympathetic ganglion development Source: UniProtKB
  61. sympathetic nervous system development Source: BHF-UCL
  62. ventral spinal cord interneuron fate commitment Source: UniProtKB
  63. vestibular nucleus development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Achaete-scute homolog 1
Short name:
ASH-1
Short name:
mASH-1
Short name:
mASH1
Gene namesi
Name:Ascl1
Synonyms:Ash1, Mash-1, Mash1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:96919. Ascl1.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. neuronal cell body Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 231231Achaete-scute homolog 1PRO_0000127127Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ02067.

PTM databases

PhosphoSiteiQ02067.

Expressioni

Tissue specificityi

Developing CNS and PNS at embryonic and post-natal stages.3 Publications

Developmental stagei

Between E8.5 and E10.5 it is found in the neuroepithelium of the midbrain and ventral forebrain, as well as in the spinal cord. Between E10.5 and E12.5 its expression pattern changes from a restricted to a widespread zone, it is then found at variable levels in the ventricular zone in all regions of the brain, where is expressed in a subset of p2 progenitors that can give rise to either V2a or V2b interneuron subtypes. From E12.5 to postnatal stages it is also expressed in cells outside of the ventricular zone through the brain, and in addition it is also expressed during development of the olfactory epithelium and neural retina.3 Publications

Gene expression databases

CleanExiMM_ASCL1.
GenevestigatoriQ02067.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3.

Protein-protein interaction databases

MINTiMINT-1520282.
STRINGi10090.ENSMUSP00000020243.

Structurei

3D structure databases

ProteinModelPortaliQ02067.
SMRiQ02067. Positions 117-169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini113 – 16553bHLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi30 – 4314Poly-AlaAdd
BLAST
Compositional biasi47 – 526Poly-Gln

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG243363.
GeneTreeiENSGT00530000063321.
HOGENOMiHOG000013056.
HOVERGENiHBG050590.
InParanoidiQ02067.
KOiK09067.
OMAiPTISPNY.
OrthoDBiEOG7RFTMH.
TreeFamiTF322889.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR015660. ASH.
IPR011598. bHLH_dom.
[Graphical view]
PANTHERiPTHR13935. PTHR13935. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAQSAQQQQ
60 70 80 90 100
PQAPPQQAPQ LSPVADSQPS GGGHKSAAKQ VKRQRSSSPE LMRCKRRLNF
110 120 130 140 150
SGFGYSLPQQ QPAAVARRNE RERNRVKLVN LGFATLREHV PNGAANKKMS
160 170 180 190 200
KVETLRSAVE YIRALQQLLD EHDAVSAAFQ AGVLSPTISP NYSNDLNSMA
210 220 230
GSPVSSYSSD EGSYDPLSPE EQELLDFTNW F
Length:231
Mass (Da):24,741
Last modified:October 3, 2012 - v2
Checksum:i454E6937520BD4CA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti81 – 811V → D in AAA37780 (PubMed:8424959).Curated
Sequence conflicti160 – 1601E → Q in AAA37780 (PubMed:8424959).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95603 mRNA. Translation: AAA37780.1.
AK143210 mRNA. Translation: BAE25307.1.
AC122360 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21455.1.
BC055748 mRNA. Translation: AAH55748.1.
CCDSiCCDS24101.1.
PIRiS28186.
RefSeqiNP_032579.2. NM_008553.4.
UniGeneiMm.136217.

Genome annotation databases

EnsembliENSMUST00000020243; ENSMUSP00000020243; ENSMUSG00000020052.
GeneIDi17172.
KEGGimmu:17172.
UCSCiuc007gqs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95603 mRNA. Translation: AAA37780.1.
AK143210 mRNA. Translation: BAE25307.1.
AC122360 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21455.1.
BC055748 mRNA. Translation: AAH55748.1.
CCDSiCCDS24101.1.
PIRiS28186.
RefSeqiNP_032579.2. NM_008553.4.
UniGeneiMm.136217.

3D structure databases

ProteinModelPortaliQ02067.
SMRiQ02067. Positions 117-169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1520282.
STRINGi10090.ENSMUSP00000020243.

PTM databases

PhosphoSiteiQ02067.

Proteomic databases

PRIDEiQ02067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020243; ENSMUSP00000020243; ENSMUSG00000020052.
GeneIDi17172.
KEGGimmu:17172.
UCSCiuc007gqs.1. mouse.

Organism-specific databases

CTDi429.
MGIiMGI:96919. Ascl1.

Phylogenomic databases

eggNOGiNOG243363.
GeneTreeiENSGT00530000063321.
HOGENOMiHOG000013056.
HOVERGENiHBG050590.
InParanoidiQ02067.
KOiK09067.
OMAiPTISPNY.
OrthoDBiEOG7RFTMH.
TreeFamiTF322889.

Miscellaneous databases

NextBioi291468.
PROiQ02067.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ASCL1.
GenevestigatoriQ02067.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR015660. ASH.
IPR011598. bHLH_dom.
[Graphical view]
PANTHERiPTHR13935. PTHR13935. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and expression of the mouse mammalian achaete-scute homolog 1 (MASH1)."
    del Amo F., Gendron-Maguire M., Gridley T.
    Biochim. Biophys. Acta 1171:323-327(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Dynamic expression of the murine Achaete-Scute homologue Mash-1 in the developing nervous system."
    Guillemot F., Joyner A.L.
    Mech. Dev. 42:171-185(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  7. "Generation of neurons by transient expression of neural bHLH proteins in mammalian cells."
    Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.
    Development 127:693-702(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  8. "Foxn4 acts synergistically with Mash1 to specify subtype identity of V2 interneurons in the spinal cord."
    Li S., Misra K., Matise M.P., Xiang M.
    Proc. Natl. Acad. Sci. U.S.A. 102:10688-10693(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEUROGENESIS, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  9. "A regulatory network involving Foxn4, Mash1 and delta-like 4/Notch1 generates V2a and V2b spinal interneurons from a common progenitor pool."
    Del Barrio M.G., Taveira-Marques R., Muroyama Y., Yuk D.I., Li S., Wines-Samuelson M., Shen J., Smith H.K., Xiang M., Rowitch D., Richardson W.D.
    Development 134:3427-3436(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEUROGENESIS.
  10. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiASCL1_MOUSE
AccessioniPrimary (citable) accession number: Q02067
Secondary accession number(s): Q7TNT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 3, 2012
Last modified: March 4, 2015
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.