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Q02067 (ASCL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Achaete-scute homolog 1

Short name=ASH-1
Short name=mASH-1
Short name=mASH1
Gene names
Name:Ascl1
Synonyms:Ash1, Mash-1, Mash1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length231 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5'-CANNTG-3'). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons. Ref.2 Ref.7 Ref.8 Ref.9

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3.

Subcellular location

Nucleus Probable.

Tissue specificity

Developing CNS and PNS at embryonic and post-natal stages. Ref.1 Ref.2 Ref.8

Developmental stage

Between E8.5 and E10.5 it is found in the neuroepithelium of the midbrain and ventral forebrain, as well as in the spinal cord. Between E10.5 and E12.5 its expression pattern changes from a restricted to a widespread zone, it is then found at variable levels in the ventricular zone in all regions of the brain, where is expressed in a subset of p2 progenitors that can give rise to either V2a or V2b interneuron subtypes. From E12.5 to postnatal stages it is also expressed in cells outside of the ventricular zone through the brain, and in addition it is also expressed during development of the olfactory epithelium and neural retina. Ref.1 Ref.2 Ref.8

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNotch signaling pathway

Inferred from genetic interaction PubMed 15976074. Source: MGI

adrenal chromaffin cell differentiation

Inferred from mutant phenotype PubMed 12361965. Source: MGI

carotid body glomus cell differentiation

Inferred from mutant phenotype PubMed 15878769. Source: MGI

cell differentiation

Inferred from mutant phenotype PubMed 10952889. Source: MGI

cell maturation

Inferred from mutant phenotype PubMed 16766700. Source: MGI

cellular response to magnetism

Inferred from electronic annotation. Source: Ensembl

central nervous system neuron development

Inferred from mutant phenotype PubMed 16469766. Source: MGI

cerebral cortex GABAergic interneuron differentiation

Inferred from electronic annotation. Source: Ensembl

cerebral cortex development

Inferred from mutant phenotype PubMed 17678855. Source: MGI

commitment of neuronal cell to specific neuron type in forebrain

Inferred from mutant phenotype PubMed 22991444. Source: MGI

enteric nervous system development

Traceable author statement PubMed 10360575. Source: BHF-UCL

generation of neurons

Inferred from mutant phenotype PubMed 23616538. Source: MGI

glial cell differentiation

Inferred from genetic interaction PubMed 15976074. Source: MGI

lung neuroendocrine cell differentiation

Inferred from mutant phenotype PubMed 9126746. Source: MGI

musculoskeletal movement, spinal reflex action

Inferred from mutant phenotype PubMed 23616538. Source: MGI

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

neuroblast fate determination

Inferred from mutant phenotype PubMed 11032813PubMed 12397111. Source: MGI

neuroblast proliferation

Inferred from genetic interaction PubMed 15976074. Source: MGI

neurogenesis

Inferred from direct assay PubMed 18184563. Source: MGI

neuron development

Inferred from mutant phenotype PubMed 8845150PubMed 9108377. Source: MGI

neuron differentiation

Inferred from mutant phenotype PubMed 15133515PubMed 16469766. Source: MGI

neuron fate commitment

Inferred from mutant phenotype PubMed 12629181PubMed 16715081PubMed 19191219. Source: MGI

neuron fate specification

Inferred from mutant phenotype PubMed 16469766. Source: MGI

neuron migration

Inferred from mutant phenotype PubMed 15976074. Source: MGI

noradrenergic neuron development

Inferred from mutant phenotype PubMed 18094025. Source: UniProtKB

noradrenergic neuron fate commitment

Inferred from electronic annotation. Source: Ensembl

olfactory pit development

Inferred from mutant phenotype PubMed 9108377. Source: MGI

oligodendrocyte cell fate commitment

Inferred from mutant phenotype PubMed 17678855. Source: MGI

oligodendrocyte development

Inferred from mutant phenotype PubMed 18287202. Source: MGI

oligodendrocyte differentiation

Inferred from mutant phenotype PubMed 18287202. Source: MGI

parasympathetic nervous system development

Traceable author statement PubMed 10360575. Source: BHF-UCL

pattern specification process

Inferred from mutant phenotype PubMed 15930101. Source: MGI

positive regulation of Notch signaling pathway

Inferred from mutant phenotype PubMed 11073877. Source: MGI

positive regulation of cell cycle

Inferred from direct assay PubMed 21536733. Source: MGI

positive regulation of neural precursor cell proliferation

Inferred from mutant phenotype PubMed 21536733. Source: MGI

positive regulation of neurogenesis

Inferred from mutant phenotype PubMed 16469766. Source: MGI

positive regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 16469766. Source: MGI

positive regulation of neuron differentiation

Inferred from direct assay Ref.7. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 17141158PubMed 23434913. Source: MGI

regulation of Notch signaling pathway

Inferred from mutant phenotype PubMed 19191219. Source: MGI

regulation of cell proliferation

Inferred from direct assay PubMed 19793887. Source: MGI

regulation of epithelial cell differentiation

Inferred from mutant phenotype PubMed 23284756. Source: MGI

regulation of gene expression

Inferred from mutant phenotype PubMed 18094025. Source: UniProtKB

regulation of mitotic cell cycle

Inferred from mutant phenotype PubMed 22991444. Source: MGI

regulation of neurogenesis

Inferred from mutant phenotype PubMed 20144606PubMed 22991444. Source: MGI

regulation of timing of subpallium neuron differentiation

Inferred from mutant phenotype PubMed 9876181. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19796622. Source: MGI

response to epidermal growth factor

Inferred from electronic annotation. Source: Ensembl

response to folic acid

Inferred from electronic annotation. Source: Ensembl

response to lithium ion

Inferred from electronic annotation. Source: Ensembl

response to retinoic acid

Inferred from electronic annotation. Source: Ensembl

spinal cord association neuron differentiation

Inferred from mutant phenotype PubMed 16715081. Source: MGI

spinal cord oligodendrocyte cell differentiation

Inferred from genetic interaction PubMed 18287202. Source: MGI

spinal cord oligodendrocyte cell fate specification

Inferred from mutant phenotype PubMed 18287202. Source: MGI

stomach neuroendocrine cell differentiation

Inferred from mutant phenotype PubMed 18173746. Source: MGI

subpallium neuron fate commitment

Inferred from mutant phenotype PubMed 9876181. Source: MGI

sympathetic ganglion development

Inferred from mutant phenotype PubMed 18094025. Source: UniProtKB

sympathetic nervous system development

Traceable author statement PubMed 10360575. Source: BHF-UCL

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

ventral spinal cord interneuron fate commitment

Inferred from mutant phenotype Ref.8. Source: UniProtKB

vestibular nucleus development

Inferred from mutant phenotype PubMed 12885554. Source: MGI

   Cellular_componentneuronal cell body

Inferred from direct assay PubMed 11133151PubMed 15065125. Source: MGI

nucleus

Inferred from direct assay PubMed 10952889PubMed 12397111PubMed 1576967PubMed 19796622. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay Ref.7. Source: UniProtKB

E-box binding

Inferred from electronic annotation. Source: Ensembl

chromatin binding

Inferred from direct assay PubMed 23639443. Source: MGI

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 23639443. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 17141158. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 17141158. Source: MGI

transcription factor binding transcription factor activity

Inferred from direct assay Ref.7. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 231231Achaete-scute homolog 1
PRO_0000127127

Regions

Domain113 – 16553bHLH
Compositional bias30 – 4314Poly-Ala
Compositional bias47 – 526Poly-Gln

Amino acid modifications

Modified residue1511N6-acetyllysine Ref.10

Experimental info

Sequence conflict811V → D in AAA37780. Ref.1
Sequence conflict1601E → Q in AAA37780. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q02067 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 454E6937520BD4CA

FASTA23124,741
        10         20         30         40         50         60 
MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAQSAQQQQ PQAPPQQAPQ 

        70         80         90        100        110        120 
LSPVADSQPS GGGHKSAAKQ VKRQRSSSPE LMRCKRRLNF SGFGYSLPQQ QPAAVARRNE 

       130        140        150        160        170        180 
RERNRVKLVN LGFATLREHV PNGAANKKMS KVETLRSAVE YIRALQQLLD EHDAVSAAFQ 

       190        200        210        220        230 
AGVLSPTISP NYSNDLNSMA GSPVSSYSSD EGSYDPLSPE EQELLDFTNW F 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and expression of the mouse mammalian achaete-scute homolog 1 (MASH1)."
del Amo F., Gendron-Maguire M., Gridley T.
Biochim. Biophys. Acta 1171:323-327(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"Dynamic expression of the murine Achaete-Scute homologue Mash-1 in the developing nervous system."
Guillemot F., Joyner A.L.
Mech. Dev. 42:171-185(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Eye.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[7]"Generation of neurons by transient expression of neural bHLH proteins in mammalian cells."
Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.
Development 127:693-702(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
[8]"Foxn4 acts synergistically with Mash1 to specify subtype identity of V2 interneurons in the spinal cord."
Li S., Misra K., Matise M.P., Xiang M.
Proc. Natl. Acad. Sci. U.S.A. 102:10688-10693(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NEUROGENESIS, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[9]"A regulatory network involving Foxn4, Mash1 and delta-like 4/Notch1 generates V2a and V2b spinal interneurons from a common progenitor pool."
Del Barrio M.G., Taveira-Marques R., Muroyama Y., Yuk D.I., Li S., Wines-Samuelson M., Shen J., Smith H.K., Xiang M., Rowitch D., Richardson W.D.
Development 134:3427-3436(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NEUROGENESIS.
[10]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95603 mRNA. Translation: AAA37780.1.
AK143210 mRNA. Translation: BAE25307.1.
AC122360 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21455.1.
BC055748 mRNA. Translation: AAH55748.1.
CCDSCCDS24101.1.
PIRS28186.
RefSeqNP_032579.2. NM_008553.4.
UniGeneMm.136217.

3D structure databases

ProteinModelPortalQ02067.
SMRQ02067. Positions 117-169.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1520282.
STRING10090.ENSMUSP00000020243.

PTM databases

PhosphoSiteQ02067.

Proteomic databases

PRIDEQ02067.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020243; ENSMUSP00000020243; ENSMUSG00000020052.
GeneID17172.
KEGGmmu:17172.
UCSCuc007gqs.1. mouse.

Organism-specific databases

CTD429.
MGIMGI:96919. Ascl1.

Phylogenomic databases

eggNOGNOG243363.
GeneTreeENSGT00530000063321.
HOGENOMHOG000013056.
HOVERGENHBG050590.
InParanoidQ02067.
KOK09067.
OMARCKRRLN.
OrthoDBEOG7RFTMH.
TreeFamTF322889.

Gene expression databases

CleanExMM_ASCL1.
GenevestigatorQ02067.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR015660. ASH.
IPR011598. bHLH_dom.
[Graphical view]
PANTHERPTHR13935. PTHR13935. 1 hit.
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291468.
PROQ02067.
SOURCESearch...

Entry information

Entry nameASCL1_MOUSE
AccessionPrimary (citable) accession number: Q02067
Secondary accession number(s): Q7TNT5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot