Q02067 (ASCL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Achaete-scute homolog 1
Short name=ASH-1
Short name=mASH-1
Short name=mASH1

Gene names

Name:	Ascl1
Synonyms:	Ash1, Mash-1, Mash1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional regulator. May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Involved in the initiation of neuronal differentiation. Mediates transcription activation by binding to the E box (5'-CANNTG-3'). Ref.2 Ref.7
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3.
Subcellular location	Nucleus Probable.
Tissue specificity	Developing CNS and PNS at embryonic and post-natal stages. Ref.1 Ref.2
Developmental stage	Between embryonic days 8.5 and 10.5 it is found in the neuroepithelium of the midbrain and ventral forebrain, as well as in the spinal cord. Between days 10.5 and 12.5 its expression pattern changes from a restricted to a widespread one, it is then found at variable levels in the ventricular zone in all regions of the brain. From day 12.5 to postnatal stages it is also expressed in cells outside of the ventricular zone through the brain, and in addition it is also expressed during development of the olfactory epithelium and neural retina. Ref.1 Ref.2
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from genetic interaction PubMed 15976074. Source: MGI adrenal chromaffin cell differentiation Inferred from mutant phenotype PubMed 12361965. Source: MGI carotid body glomus cell differentiation Inferred from mutant phenotype PubMed 15878769. Source: MGI cellular response to magnetism Inferred from electronic annotation. Source: Compara central nervous system neuron development Inferred from mutant phenotype PubMed 16469766. Source: MGI cerebral cortex GABAergic interneuron differentiation Inferred from electronic annotation. Source: Compara cerebral cortex development Inferred from mutant phenotype PubMed 17678855. Source: MGI enteric nervous system development Traceable author statement PubMed 10360575. Source: BHF-UCL forebrain neuron differentiation Inferred from electronic annotation. Source: Compara lung neuroendocrine cell differentiation Inferred from mutant phenotype PubMed 9126746. Source: MGI negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of neuron differentiation Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara neuroblast fate determination Inferred from mutant phenotype PubMed 11032813PubMed 12397111. Source: MGI neuroblast proliferation Inferred from genetic interaction PubMed 15976074. Source: MGI neuron fate specification Inferred from mutant phenotype PubMed 16469766. Source: MGI neuron migration Inferred from mutant phenotype PubMed 15976074. Source: MGI noradrenergic neuron fate commitment Inferred from electronic annotation. Source: Compara olfactory pit development Inferred from mutant phenotype PubMed 9108377. Source: MGI oligodendrocyte development Inferred from mutant phenotype PubMed 18287202. Source: MGI parasympathetic nervous system development Traceable author statement PubMed 10360575. Source: BHF-UCL pattern specification process Inferred from mutant phenotype PubMed 15930101. Source: MGI positive regulation of Notch signaling pathway Inferred from mutant phenotype PubMed 11073877. Source: MGI positive regulation of cell cycle Inferred from direct assay PubMed 21536733. Source: MGI positive regulation of neural precursor cell proliferation Inferred from mutant phenotype PubMed 21536733. Source: MGI positive regulation of neurogenesis Inferred from mutant phenotype PubMed 16469766. Source: MGI positive regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 16469766. Source: MGI positive regulation of neuron differentiation Inferred from direct assay Ref.7. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 17141158. Source: MGI regulation of timing of subpallium neuron differentiation Inferred from mutant phenotype PubMed 9876181. Source: MGI response to epidermal growth factor stimulus Inferred from electronic annotation. Source: Compara response to folic acid Inferred from electronic annotation. Source: Compara response to lithium ion Inferred from electronic annotation. Source: Compara response to retinoic acid Inferred from electronic annotation. Source: Compara spinal cord association neuron differentiation Inferred from mutant phenotype PubMed 16715081. Source: MGI spinal cord oligodendrocyte cell fate specification Inferred from mutant phenotype PubMed 18287202. Source: MGI stomach neuroendocrine cell differentiation Inferred from mutant phenotype PubMed 18173746. Source: MGI subpallium neuron fate commitment Inferred from mutant phenotype PubMed 9876181. Source: MGI sympathetic nervous system development Traceable author statement PubMed 10360575. Source: BHF-UCL transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW vestibular nucleus development Inferred from mutant phenotype PubMed 12885554. Source: MGI
Cellular_component	neuronal cell body Inferred from direct assay PubMed 11133151PubMed 15065125. Source: MGI nucleus Inferred from direct assay PubMed 10952889PubMed 12397111PubMed 1576967PubMed 19796622. Source: MGI
Molecular_function	E-box binding Inferred from electronic annotation. Source: Compara double-stranded DNA binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding Inferred from direct assay PubMed 17141158. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 17141158. Source: MGI transcription factor binding transcription factor activity Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Achaete-scute homolog 1		PRO_0000127127
Regions
	Domain	113 – 165	53	bHLH
	Compositional bias	30 – 43	14	Poly-Ala
	Compositional bias	47 – 52	6	Poly-Gln
Experimental info
	Sequence conflict	81	1	V → D in AAA37780. Ref.1
	Sequence conflict	160	1	E → Q in AAA37780. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q02067 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 454E6937520BD4CA	FASTA	231	24,741
	10 20 30 40 50 60 MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAQSAQQQQ PQAPPQQAPQ 70 80 90 100 110 120 LSPVADSQPS GGGHKSAAKQ VKRQRSSSPE LMRCKRRLNF SGFGYSLPQQ QPAAVARRNE 130 140 150 160 170 180 RERNRVKLVN LGFATLREHV PNGAANKKMS KVETLRSAVE YIRALQQLLD EHDAVSAAFQ 190 200 210 220 230 AGVLSPTISP NYSNDLNSMA GSPVSSYSSD EGSYDPLSPE EQELLDFTNW F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and expression of the mouse mammalian achaete-scute homolog 1 (MASH1)." del Amo F., Gendron-Maguire M., Gridley T. Biochim. Biophys. Acta 1171:323-327(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Dynamic expression of the murine Achaete-Scute homologue Mash-1 in the developing nervous system." Guillemot F., Joyner A.L. Mech. Dev. 42:171-185(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Eye.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[7]	"Generation of neurons by transient expression of neural bHLH proteins in mammalian cells." Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L. Development 127:693-702(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DNA-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95603 mRNA. Translation: AAA37780.1. AK143210 mRNA. Translation: BAE25307.1. AC122360 Genomic DNA. No translation available. CH466539 Genomic DNA. Translation: EDL21455.1. BC055748 mRNA. Translation: AAH55748.1.
IPI	IPI00123359. IPI00396801.
PIR	S28186.
RefSeq	NP_032579.2. NM_008553.4.
UniGene	Mm.136217.
3D structure databases
ProteinModelPortal	Q02067.
SMR	Q02067. Positions 117-169.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000020243.
PTM databases
PhosphoSite	Q02067.
Proteomic databases
PRIDE	Q02067.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020243; ENSMUSP00000020243; ENSMUSG00000020052.
GeneID	17172.
KEGG	mmu:17172.
Organism-specific databases
CTD	429.
MGI	MGI:96919. Ascl1.
Phylogenomic databases
eggNOG	NOG243363.
GeneTree	ENSGT00530000063321.
HOGENOM	HOG000013056.
HOVERGEN	HBG050590.
InParanoid	Q02067.
KO	K09067.
OMA	RCKRRLN.
OrthoDB	EOG42BX9T.
Gene expression databases
CleanEx	MM_ASCL1.
Genevestigator	Q02067.
GermOnline	ENSMUSG00000020052. Mus musculus.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR015660. ASH. IPR011598. bHLH_dom. [Graphical view]
PANTHER	PTHR13935. PTHR13935. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291468.
SOURCE	Search...

Entry information

Entry name

ASCL1_MOUSE

Accession

Primary (citable) accession number: Q02067
Secondary accession number(s): Q7TNT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program