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Q02067

- ASCL1_MOUSE

UniProt

Q02067 - ASCL1_MOUSE

Protein

Achaete-scute homolog 1

Gene

Ascl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5'-CANNTG-3'). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons.4 Publications

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: UniProtKB
    3. double-stranded DNA binding Source: Ensembl
    4. E-box binding Source: Ensembl
    5. protein binding Source: MGI
    6. sequence-specific DNA binding Source: MGI
    7. sequence-specific DNA binding transcription factor activity Source: MGI
    8. transcription factor binding transcription factor activity Source: UniProtKB

    GO - Biological processi

    1. adrenal chromaffin cell differentiation Source: MGI
    2. carotid body glomus cell differentiation Source: MGI
    3. cell differentiation Source: MGI
    4. cell maturation Source: MGI
    5. cellular response to magnetism Source: Ensembl
    6. central nervous system neuron development Source: MGI
    7. cerebral cortex development Source: MGI
    8. cerebral cortex GABAergic interneuron differentiation Source: Ensembl
    9. commitment of neuronal cell to specific neuron type in forebrain Source: MGI
    10. enteric nervous system development Source: BHF-UCL
    11. generation of neurons Source: MGI
    12. glial cell differentiation Source: MGI
    13. lung neuroendocrine cell differentiation Source: MGI
    14. musculoskeletal movement, spinal reflex action Source: MGI
    15. negative regulation of apoptotic process Source: Ensembl
    16. negative regulation of neuron differentiation Source: Ensembl
    17. negative regulation of transcription, DNA-templated Source: Ensembl
    18. neuroblast fate determination Source: MGI
    19. neuroblast proliferation Source: MGI
    20. neurogenesis Source: MGI
    21. neuron development Source: MGI
    22. neuron differentiation Source: MGI
    23. neuron fate commitment Source: MGI
    24. neuron fate specification Source: MGI
    25. neuron migration Source: MGI
    26. noradrenergic neuron development Source: UniProtKB
    27. noradrenergic neuron fate commitment Source: Ensembl
    28. Notch signaling pathway Source: MGI
    29. olfactory pit development Source: MGI
    30. oligodendrocyte cell fate commitment Source: MGI
    31. oligodendrocyte development Source: MGI
    32. oligodendrocyte differentiation Source: MGI
    33. parasympathetic nervous system development Source: BHF-UCL
    34. pattern specification process Source: MGI
    35. positive regulation of cell cycle Source: MGI
    36. positive regulation of neural precursor cell proliferation Source: MGI
    37. positive regulation of neurogenesis Source: MGI
    38. positive regulation of neuron apoptotic process Source: MGI
    39. positive regulation of neuron differentiation Source: UniProtKB
    40. positive regulation of Notch signaling pathway Source: MGI
    41. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    42. regulation of cell proliferation Source: MGI
    43. regulation of epithelial cell differentiation Source: MGI
    44. regulation of gene expression Source: UniProtKB
    45. regulation of mitotic cell cycle Source: MGI
    46. regulation of neurogenesis Source: MGI
    47. regulation of Notch signaling pathway Source: MGI
    48. regulation of timing of subpallium neuron differentiation Source: MGI
    49. regulation of transcription from RNA polymerase II promoter Source: MGI
    50. response to epidermal growth factor Source: Ensembl
    51. response to folic acid Source: Ensembl
    52. response to lithium ion Source: Ensembl
    53. response to retinoic acid Source: Ensembl
    54. spinal cord association neuron differentiation Source: MGI
    55. spinal cord oligodendrocyte cell differentiation Source: MGI
    56. spinal cord oligodendrocyte cell fate specification Source: MGI
    57. stomach neuroendocrine cell differentiation Source: MGI
    58. subpallium neuron fate commitment Source: MGI
    59. sympathetic ganglion development Source: UniProtKB
    60. sympathetic nervous system development Source: BHF-UCL
    61. transcription, DNA-templated Source: UniProtKB-KW
    62. ventral spinal cord interneuron fate commitment Source: UniProtKB
    63. vestibular nucleus development Source: MGI

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Achaete-scute homolog 1
    Short name:
    ASH-1
    Short name:
    mASH-1
    Short name:
    mASH1
    Gene namesi
    Name:Ascl1
    Synonyms:Ash1, Mash-1, Mash1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:96919. Ascl1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. neuronal cell body Source: MGI
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 231231Achaete-scute homolog 1PRO_0000127127Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei151 – 1511N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ02067.

    PTM databases

    PhosphoSiteiQ02067.

    Expressioni

    Tissue specificityi

    Developing CNS and PNS at embryonic and post-natal stages.3 Publications

    Developmental stagei

    Between E8.5 and E10.5 it is found in the neuroepithelium of the midbrain and ventral forebrain, as well as in the spinal cord. Between E10.5 and E12.5 its expression pattern changes from a restricted to a widespread zone, it is then found at variable levels in the ventricular zone in all regions of the brain, where is expressed in a subset of p2 progenitors that can give rise to either V2a or V2b interneuron subtypes. From E12.5 to postnatal stages it is also expressed in cells outside of the ventricular zone through the brain, and in addition it is also expressed during development of the olfactory epithelium and neural retina.3 Publications

    Gene expression databases

    CleanExiMM_ASCL1.
    GenevestigatoriQ02067.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3.

    Protein-protein interaction databases

    MINTiMINT-1520282.
    STRINGi10090.ENSMUSP00000020243.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02067.
    SMRiQ02067. Positions 117-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini113 – 16553bHLHPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi30 – 4314Poly-AlaAdd
    BLAST
    Compositional biasi47 – 526Poly-Gln

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG243363.
    GeneTreeiENSGT00530000063321.
    HOGENOMiHOG000013056.
    HOVERGENiHBG050590.
    InParanoidiQ02067.
    KOiK09067.
    OMAiRCKRRLN.
    OrthoDBiEOG7RFTMH.
    TreeFamiTF322889.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR015660. ASH.
    IPR011598. bHLH_dom.
    [Graphical view]
    PANTHERiPTHR13935. PTHR13935. 1 hit.
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02067-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAQSAQQQQ    50
    PQAPPQQAPQ LSPVADSQPS GGGHKSAAKQ VKRQRSSSPE LMRCKRRLNF 100
    SGFGYSLPQQ QPAAVARRNE RERNRVKLVN LGFATLREHV PNGAANKKMS 150
    KVETLRSAVE YIRALQQLLD EHDAVSAAFQ AGVLSPTISP NYSNDLNSMA 200
    GSPVSSYSSD EGSYDPLSPE EQELLDFTNW F 231
    Length:231
    Mass (Da):24,741
    Last modified:October 3, 2012 - v2
    Checksum:i454E6937520BD4CA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti81 – 811V → D in AAA37780. (PubMed:8424959)Curated
    Sequence conflicti160 – 1601E → Q in AAA37780. (PubMed:8424959)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95603 mRNA. Translation: AAA37780.1.
    AK143210 mRNA. Translation: BAE25307.1.
    AC122360 Genomic DNA. No translation available.
    CH466539 Genomic DNA. Translation: EDL21455.1.
    BC055748 mRNA. Translation: AAH55748.1.
    CCDSiCCDS24101.1.
    PIRiS28186.
    RefSeqiNP_032579.2. NM_008553.4.
    UniGeneiMm.136217.

    Genome annotation databases

    EnsembliENSMUST00000020243; ENSMUSP00000020243; ENSMUSG00000020052.
    GeneIDi17172.
    KEGGimmu:17172.
    UCSCiuc007gqs.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95603 mRNA. Translation: AAA37780.1 .
    AK143210 mRNA. Translation: BAE25307.1 .
    AC122360 Genomic DNA. No translation available.
    CH466539 Genomic DNA. Translation: EDL21455.1 .
    BC055748 mRNA. Translation: AAH55748.1 .
    CCDSi CCDS24101.1.
    PIRi S28186.
    RefSeqi NP_032579.2. NM_008553.4.
    UniGenei Mm.136217.

    3D structure databases

    ProteinModelPortali Q02067.
    SMRi Q02067. Positions 117-169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1520282.
    STRINGi 10090.ENSMUSP00000020243.

    PTM databases

    PhosphoSitei Q02067.

    Proteomic databases

    PRIDEi Q02067.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020243 ; ENSMUSP00000020243 ; ENSMUSG00000020052 .
    GeneIDi 17172.
    KEGGi mmu:17172.
    UCSCi uc007gqs.1. mouse.

    Organism-specific databases

    CTDi 429.
    MGIi MGI:96919. Ascl1.

    Phylogenomic databases

    eggNOGi NOG243363.
    GeneTreei ENSGT00530000063321.
    HOGENOMi HOG000013056.
    HOVERGENi HBG050590.
    InParanoidi Q02067.
    KOi K09067.
    OMAi RCKRRLN.
    OrthoDBi EOG7RFTMH.
    TreeFami TF322889.

    Miscellaneous databases

    NextBioi 291468.
    PROi Q02067.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_ASCL1.
    Genevestigatori Q02067.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR015660. ASH.
    IPR011598. bHLH_dom.
    [Graphical view ]
    PANTHERi PTHR13935. PTHR13935. 1 hit.
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and expression of the mouse mammalian achaete-scute homolog 1 (MASH1)."
      del Amo F., Gendron-Maguire M., Gridley T.
      Biochim. Biophys. Acta 1171:323-327(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    2. "Dynamic expression of the murine Achaete-Scute homologue Mash-1 in the developing nervous system."
      Guillemot F., Joyner A.L.
      Mech. Dev. 42:171-185(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    7. "Generation of neurons by transient expression of neural bHLH proteins in mammalian cells."
      Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.
      Development 127:693-702(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.
    8. "Foxn4 acts synergistically with Mash1 to specify subtype identity of V2 interneurons in the spinal cord."
      Li S., Misra K., Matise M.P., Xiang M.
      Proc. Natl. Acad. Sci. U.S.A. 102:10688-10693(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN NEUROGENESIS, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    9. "A regulatory network involving Foxn4, Mash1 and delta-like 4/Notch1 generates V2a and V2b spinal interneurons from a common progenitor pool."
      Del Barrio M.G., Taveira-Marques R., Muroyama Y., Yuk D.I., Li S., Wines-Samuelson M., Shen J., Smith H.K., Xiang M., Rowitch D., Richardson W.D.
      Development 134:3427-3436(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN NEUROGENESIS.
    10. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiASCL1_MOUSE
    AccessioniPrimary (citable) accession number: Q02067
    Secondary accession number(s): Q7TNT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3