Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-directed RNA polymerase II subunit RPB2

Gene

rpb2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).By similarity

Miscellaneous

The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi826Magnesium; shared with RPB1By similarity1
Metal bindingi1152ZincBy similarity1
Metal bindingi1155ZincBy similarity1
Metal bindingi1170ZincBy similarity1
Metal bindingi1173ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1152 – 1173C4-typeAdd BLAST22

GO - Molecular functioni

  • DNA binding Source: PomBase
  • DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ribonucleoside binding Source: InterPro
  • RNA binding Source: PomBase

GO - Biological processi

  • chromatin silencing by small RNA Source: PomBase
  • transcription by RNA polymerase II Source: PomBase

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-113418 Formation of the Early Elongation Complex
R-SPO-5578749 Transcriptional regulation by small RNAs
R-SPO-674695 RNA Polymerase II Pre-transcription Events
R-SPO-6781823 Formation of TC-NER Pre-Incision Complex
R-SPO-6782135 Dual incision in TC-NER
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SPO-72086 mRNA Capping
R-SPO-72165 mRNA Splicing - Minor Pathway
R-SPO-73776 RNA Polymerase II Promoter Escape
R-SPO-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SPO-75953 RNA Polymerase II Transcription Initiation
R-SPO-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SPO-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-SPO-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB2 (EC:2.7.7.6)
Short name:
RNA polymerase II subunit 2
Short name:
RNA polymerase II subunit B2
Alternative name(s):
DNA-directed RNA polymerase II 138 kDa polypeptide
Gene namesi
Name:rpb2
ORF Names:SPAC23G3.01, SPAC521.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23G3.01
PomBaseiSPAC23G3.01 rpb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480901 – 1210DNA-directed RNA polymerase II subunit RPB2Add BLAST1210

Proteomic databases

MaxQBiQ02061
PaxDbiQ02061
PRIDEiQ02061

PTM databases

iPTMnetiQ02061

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.By similarity

Protein-protein interaction databases

BioGridi277951, 19 interactors
IntActiQ02061, 2 interactors
MINTiQ02061
STRINGi4896.SPAC23G3.01.1

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65B/N1-1210[»]
5U0Selectron microscopy7.80b1-1210[»]
ProteinModelPortaliQ02061
SMRiQ02061
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02061

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1152 – 1173C4-typeAdd BLAST22

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000222962
InParanoidiQ02061
KOiK03010
OMAiKNLSLMC
OrthoDBiEOG092C06IY
PhylomeDBiQ02061

Family and domain databases

CDDicd00653 RNA_pol_B_RPB2, 1 hit
Gene3Di2.40.270.10, 3 hits
2.40.50.150, 2 hits
3.90.1110.10, 1 hit
InterProiView protein in InterPro
IPR015712 DNA-dir_RNA_pol_su2
IPR007120 DNA-dir_RNAP_su2_dom
IPR037033 DNA-dir_RNAP_su2_hyb_sf
IPR007121 RNA_pol_bsu_CS
IPR007644 RNA_pol_bsu_protrusion
IPR007642 RNA_pol_Rpb2_2
IPR037034 RNA_pol_Rpb2_2_sf
IPR007645 RNA_pol_Rpb2_3
IPR007646 RNA_pol_Rpb2_4
IPR007647 RNA_pol_Rpb2_5
IPR007641 RNA_pol_Rpb2_7
IPR014724 RNA_pol_RPB2_OB-fold
PANTHERiPTHR20856 PTHR20856, 1 hit
PfamiView protein in Pfam
PF04563 RNA_pol_Rpb2_1, 1 hit
PF04561 RNA_pol_Rpb2_2, 1 hit
PF04565 RNA_pol_Rpb2_3, 1 hit
PF04566 RNA_pol_Rpb2_4, 1 hit
PF04567 RNA_pol_Rpb2_5, 1 hit
PF00562 RNA_pol_Rpb2_6, 1 hit
PF04560 RNA_pol_Rpb2_7, 1 hit
PROSITEiView protein in PROSITE
PS01166 RNA_POL_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

Q02061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYEDYQYNE TLTQEDCWTV ISSFFEETSL ARQQLFSFDE FVQNTMQEIV
60 70 80 90 100
DDDSTLTLDQ YAQHTGAQGD VTRRYEINFG QIYLSRPTMT EADGSTTTMF
110 120 130 140 150
PQEARLRNLT YSSPLYVDMR KKVMVAADSN VPIGEEEWLV EEEDEEPSKV
160 170 180 190 200
FIGKIPIMLR STFCILNGVS DSELYDLNEC PYDQGGYFII NGSEKVIIAQ
210 220 230 240 250
ERSAANIVQV FKKAAPSPIA YVAEIRSALE RGSRLISSMQ IKLMARNTEN
260 270 280 290 300
SGQTIRATLP YIRSDIPIVI VFRALGVVPD RDILEHICYD PNDFQMLEMM
310 320 330 340 350
KPCIEEAFVI QDKDIALDYI GKRGSTTGVT REKRLRYAHD ILQKELLPHI
360 370 380 390 400
TTMEGFETRK AFFLGYMIHR MLLCALERRE PDDRDHFGKK RLDLAGPLLA
410 420 430 440 450
SLFRMLFRKM TRDVYKYMQK CVETNREFNL TLAVKSNIIT NGLRYSLATG
460 470 480 490 500
NWGDQKRSMV NRVGVSQVLN RYTFASTLSH LRRTNTPIGR DGKLAKPRQL
510 520 530 540 550
HNTHWGMVCP AETPEGQACG LVKNLSLMSY VSVGSPSAPI IEFLEEWGLE
560 570 580 590 600
TLEDYNPSAS PNATKVFVNG VWLGVHRDPA HLTETLRSLR RRLDISAEVS
610 620 630 640 650
IVRDIREKEL RLFTDAGRIC RPLFIVDNNP NSERRGELCI RKEHIQQLIE
660 670 680 690 700
DKDRYDIDPE QRFGWTALVS SGLIEYLDAE EEETVMIAMS PEDLEASRQM
710 720 730 740 750
QAGYEVKEEL DPAQRVKPAP NPHVHAWTHC EIHPAMILGI LASIIPFPDH
760 770 780 790 800
NQSPRNTYQS AMGKQAMGVY LTNYQVRMDT MANILYYPQK PLATTRSMEY
810 820 830 840 850
LKFRELPAGQ NAIVAILCYS GYNQEDSIIM NQASIDRGLF RSIFYRTYTD
860 870 880 890 900
QEKKIGMTVM EEFERPVRST TLRMKHGTYD KLEDDGLIAP GTRVSGEDII
910 920 930 940 950
IGKTAPIPLD HEELGQRTQL HAKRDVSTPL RSTESGIVDQ VMVTTNQEGL
960 970 980 990 1000
KFVKVRMRST RIPQIGDKFA SRHGQKGTIG MTYRHEDMPF SAQGIVPDII
1010 1020 1030 1040 1050
INPHAIPSRM TVAHLVECQL SKVSALSGFE GDATPFTDVT VEAVSKLLRS
1060 1070 1080 1090 1100
HGFQSRGFEV MYHGHTGRKL VAQVFLGPTY YQRLKHLVDD KIHARARGPV
1110 1120 1130 1140 1150
QILTRQPVEG RSRDGGLRFG EMERDCQISH GCSSVLRERL FDCSDAYRVI
1160 1170 1180 1190 1200
VCDICGLIAI ASYKKDSYEC RSCQNRTRFS QVYLPYAAKL LFQELMSMNI
1210
APRLFTKNHK
Length:1,210
Mass (Da):137,849
Last modified:August 14, 2001 - v2
Checksum:iC5D3794A743494CC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti727W → R in BAA02600 (PubMed:8441660).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13337 Genomic DNA Translation: BAA02600.1
CU329670 Genomic DNA Translation: CAB86470.1
PIRiS35548
T50175
RefSeqiNP_593101.2, NM_001018498.2

Genome annotation databases

EnsemblFungiiSPAC23G3.01.1; SPAC23G3.01.1:pep; SPAC23G3.01
GeneIDi2541446
KEGGispo:SPAC23G3.01

Similar proteinsi

Entry informationi

Entry nameiRPB2_SCHPO
AccessioniPrimary (citable) accession number: Q02061
Secondary accession number(s): Q9P7B1, Q9P7T2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 14, 2001
Last modified: May 23, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health