Q02059 (KASA_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actinorhodin polyketide putative beta-ketoacyl synthase 1
EC=2.3.1.-

Alternative name(s):
actI ORF1

Gene names

Ordered Locus Names:	SCO5087
ORF Names:	SCBAC28G1.13

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Pathway	Antibiotic biosynthesis; actinorhodin biosynthesis.
Sequence similarities	Belongs to the beta-ketoacyl-ACP synthases family.
Sequence caution	The sequence CAA45043.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 467

467

Actinorhodin polyketide putative beta-ketoacyl synthase 1

PRO_0000180342

Sites

Active site

212

1

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

467

Helix Strand Turn

Details...

Beta strand

48 – 57

10

Beta strand

60 – 62

3

Helix

63 – 72

10

Beta strand

77 – 79

3

Beta strand

92 – 94

3

Helix

100 – 103

4

Helix

107 – 112

6

Helix

115 – 131

17

Turn

135 – 137

3

Helix

140 – 142

3

Beta strand

143 – 148

6

Helix

154 – 165

12

Turn

166 – 169

4

Helix

175 – 177

3

Turn

180 – 182

3

Helix

183 – 186

4

Helix

190 – 199

10

Beta strand

205 – 208

4

Helix

211 – 213

3

Helix

214 – 227

14

Beta strand

232 – 240

9

Helix

245 – 253

9

Helix

264 – 266

3

Beta strand

283 – 291

9

Helix

292 – 297

6

Beta strand

304 – 313

10

Helix

326 – 339

14

Helix

343 – 345

3

Beta strand

348 – 350

3

Helix

357 – 370

14

Helix

372 – 377

6

Helix

384 – 387

4

Turn

391 – 393

3

Helix

394 – 408

15

Beta strand

419 – 421

3

Beta strand

430 – 432

3

Beta strand

439 – 447

9

Turn

448 – 450

3

Beta strand

451 – 458

8

Helix

460 – 465

6

Sequences

	Sequence		Length	Mass (Da)
	Q02059 [UniParc]. Last modified January 17, 2003. Version 2. Checksum: 6B5BEE32B5241C60	FASTA	467	49,504
	10 20 30 40 50 60 MPLDAAPVDP ASRGPVSAFE PPSSHGADDD DDHRTNASKE LFGLKRRVVI TGVGVRAPGG 70 80 90 100 110 120 NGTRQFWELL TSGRTATRRI SFFDPSPYRS QVAAEADFDP VAEGFGPREL DRMDRASQFA 130 140 150 160 170 180 VACAREAFAA SGLDPDTLDP ARVGVSLGSA VAAATSLERE YLLLSDSGRD WEVDAAWLSR 190 200 210 220 230 240 HMFDYLVPSV MPAEVAWAVG AEGPVTMVST GCTSGLDSVG NAVRAIEEGS ADVMFAGAAD 250 260 270 280 290 300 TPITPIVVAC FDAIRATTAR NDDPEHASRP FDGTRDGFVL AEGAAMFVLE DYDSALARGA 310 320 330 340 350 360 RIHAEISGYA TRCNAYHMTG LKADGREMAE TIRVALDESR TDATDIDYIN AHGSGTRQND 370 380 390 400 410 420 RHETAAYKRA LGEHARRTPV SSIKSMVGHS LGAIGSLEIA ACVLALEHGV VPPTANLRTS 430 440 450 460 DPECDLDYVP LEARERKLRS VLTVGSGFGG FQSAMVLRDA ETAGAAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[2]	"Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin." Fernandez-Moreno M.A., Martinez E., Boto L., Hopwood D.A., Malpartida F. J. Biol. Chem. 267:19278-19290(1992) [PubMed: 1527048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-467. Strain: ATCC BAA-471 / A3(2) / M145.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL939122 Genomic DNA. Translation: CAC44200.1.
X63449 Genomic DNA. Translation: CAA45043.1. Different initiation.

PIR

S25840.

RefSeq

NP_629237.1. NC_003888.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QXG	model	-	A	45-461	[»]
1TQY	X-ray	2.00	A/C/E/G	45-467	[»]

ProteinModelPortal

Q02059.

SMR

Q02059. Positions 46-466.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q02059. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1100528.

GenomeReviews

Gene locus SCO5087 in contig AL645882_GR.

KEGG

sco:SCO5087.

NMPDR

fig|100226.1.peg.5042.

PATRIC

23740014. VBIStrCoe124346_5167.

Phylogenomic databases

HOGENOM

HBG757733.

OMA

PITSACF.

PhylomeDB

Q02059.

ProtClustDB

CLSK902850.

Family and domain databases

InterPro

IPR000794. Beta-ketoacyl_synthase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.

KO

K05551.

PANTHER

PTHR11712. Ketoacyl_synth. 1 hit.

Pfam

PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]

SUPFAM

SSF53901. Thiolase-like. 1 hit.

PROSITE

PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KASA_STRCO

Accession

Primary (citable) accession number: Q02059
Secondary accession number(s): Q93IZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 17, 2003
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program