Q02047 (OTC1_PSESH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase 1, phaseolotoxin-sensitive
Short name=OTCase 1
EC=2.1.3.3

Alternative name(s):
SOCT

Gene names

Name:

argF

Organism

Pseudomonas syringae pv. phaseolicola

Taxonomic identifier

319 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subunit structure	Homotrimer.
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Induction	Negatively regulated by ArgR. Ref.4
Miscellaneous	Optimum pH is 8.5-9.5. HAMAP MF_01109 This enzyme is highly inhibited by phaseolotoxin, a potent inhibitor of OTCase. HAMAP MF_01109
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 306	305	Ornithine carbamoyltransferase 1, phaseolotoxin-sensitive HAMAP MF_01109		PRO_0000112991
Regions
	Region	53 – 57	5	Carbamoyl phosphate binding By similarity
	Region	258 – 261	4	Ornithine binding By similarity
Sites
	Binding site	104	1	Carbamoyl phosphate By similarity
	Binding site	131	1	Carbamoyl phosphate By similarity
	Site	28	1	Important for structural integrity By similarity
	Site	144	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02047 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D37B503563D53F50	FASTA	306	34,151
	10 20 30 40 50 60 MNARHFLSMM DYTPDELLGL IRRGVELKDL RIRGELFEPL KNRVLGMIFE KSSTRTRLSF 70 80 90 100 110 120 EAGMIQLGGQ AIFLSHRDTQ LGRGEPIADS AKVMSRMLDA VMIRTYAHSN LTEFAANSRV 130 140 150 160 170 180 PVINGLSDDL HPCQLLADMQ TFLEHRGSIK GKTVAWIGDG NNMCNSYIEA AIQFDFQLRV 190 200 210 220 230 240 ACPAGYEPNP EFLALAGERV TIVRDPKAAV AGAHLVSTDV WTSMGQEEET ARRMALFAPF 250 260 270 280 290 300 QVTRASLDLA EKDVLFMHCL PAHRGEEISV DLLDDSRSVA WDQAENRLHA QKALLEFLVA PSHQRA

References

[1]	"Origin, structure, and regulation of argK, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola, and functional expression of argK in transgenic tobacco." Hatziloukas E., Panopoulos N.J. J. Bacteriol. 174:5895-5909(1992) [PubMed: 1522066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 4419.
[2]	Mosqueda-Cano G. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate Texcoco.
[3]	"Characterization of two ornithine carbamoyltransferases from Pseudomonas syringae pv. phaseolicola, the producer of phaseolotoxin." Jahn O., Sauerstein J., Reuter G. Arch. Microbiol. 147:174-178(1987) [PubMed: 3592910] [Abstract] Cited for: CHARACTERIZATION.
[4]	"The global arginine regulator ArgR controls expression of argF in Pseudomonas syringae pv. phaseolicola but is not required for the synthesis of phaseolotoxin or for the regulated expression of argK." Hernandez-Flores J.L., Lopez-Lopez K., Garciduenas-Pina R., Jofre-Garfias A.E., Alvarez-Morales A. J. Bacteriol. 186:3653-3655(2004) [PubMed: 15150254] [Abstract] Cited for: REGULATION BY ARGR.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M99382 Genomic DNA. Translation: AAA25909.1. X76945 Genomic DNA. Translation: CAA54264.1.
PIR	S41291.
3D structure databases
ProteinModelPortal	Q02047.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC1_PSESH

Accession

Primary (citable) accession number: Q02047

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program