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Protein

Methylenetetrahydrofolate dehydrogenase [NAD(+)]

Gene

MTD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis.

Catalytic activityi

5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei150By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 186NAD1 Publication2
Nucleotide bindingi208 – 209NAD1 Publication2
Nucleotide bindingi274 – 276NAD1 Publication3

GO - Molecular functioni

  • methylenetetrahydrofolate dehydrogenase (NAD+) activity Source: SGD

GO - Biological processi

  • folic acid-containing compound biosynthetic process Source: SGD
  • one-carbon metabolic process Source: SGD
  • purine nucleobase biosynthetic process Source: SGD
  • purine nucleotide biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YKR080W-MONOMER.
SABIO-RKQ02046.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate dehydrogenase [NAD(+)] (EC:1.5.1.15)
Gene namesi
Name:MTD1
Ordered Locus Names:YKR080W
ORF Names:YKR400
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR080W.
SGDiS000001788. MTD1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001993191 – 320Methylenetetrahydrofolate dehydrogenase [NAD(+)]Add BLAST320

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiQ02046.
PRIDEiQ02046.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi34211. 16 interactors.
DIPiDIP-730N.
IntActiQ02046. 1 interactor.
MINTiMINT-4493839.

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 31Combined sources22
Turni32 – 34Combined sources3
Beta strandi38 – 43Combined sources6
Helixi48 – 63Combined sources16
Beta strandi67 – 72Combined sources6
Helixi76 – 78Combined sources3
Helixi79 – 88Combined sources10
Beta strandi94 – 97Combined sources4
Beta strandi101 – 104Combined sources4
Helixi105 – 110Combined sources6
Turni111 – 113Combined sources3
Turni116 – 118Combined sources3
Beta strandi119 – 121Combined sources3
Helixi125 – 132Combined sources8
Beta strandi137 – 142Combined sources6
Helixi150 – 161Combined sources12
Turni174 – 177Combined sources4
Beta strandi179 – 183Combined sources5
Turni187 – 189Combined sources3
Helixi190 – 198Combined sources9
Beta strandi203 – 207Combined sources5
Beta strandi209 – 217Combined sources9
Beta strandi227 – 233Combined sources7
Helixi236 – 245Combined sources10
Beta strandi247 – 251Combined sources5
Turni262 – 264Combined sources3
Beta strandi269 – 273Combined sources5
Beta strandi275 – 277Combined sources3
Helixi282 – 285Combined sources4
Beta strandi288 – 293Combined sources6
Helixi296 – 316Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EDZX-ray2.80A1-320[»]
1EE9X-ray3.00A1-320[»]
ProteinModelPortaliQ02046.
SMRiQ02046.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02046.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000158212.
InParanoidiQ02046.
KOiK00295.
OMAiVYYPIFP.
OrthoDBiEOG092C3E8S.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPGRTILA SKVAETFNTE IINNVEEYKK THNGQGPLLV GFLANNDPAA
60 70 80 90 100
KMYATWTQKT SESMGFRYDL RVIEDKDFLE EAIIQANGDD SVNGIMVYFP
110 120 130 140 150
VFGNAQDQYL QQVVCKEKDV EGLNHVYYQN LYHNVRYLDK ENRLKSILPC
160 170 180 190 200
TPLAIVKILE FLKIYNNLLP EGNRLYGKKC IVINRSEIVG RPLAALLAND
210 220 230 240 250
GATVYSVDVN NIQKFTRGES LKLNKHHVED LGEYSEDLLK KCSLDSDVVI
260 270 280 290 300
TGVPSENYKF PTEYIKEGAV CINFACTKNF SDDVKEKASL YVPMTGKVTI
310 320
AMLLRNMLRL VRNVELSKEK
Length:320
Mass (Da):36,240
Last modified:October 1, 1993 - v1
Checksum:iDA30D9E2CEB590C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02934 Genomic DNA. Translation: AAB00323.1.
Z27116 Genomic DNA. Translation: CAA81631.1.
Z28305 Genomic DNA. Translation: CAA82159.1.
BK006944 Genomic DNA. Translation: DAA09230.1.
PIRiS31254.
RefSeqiNP_013006.3. NM_001179870.3.

Genome annotation databases

EnsemblFungiiYKR080W; YKR080W; YKR080W.
GeneIDi853955.
KEGGisce:YKR080W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02934 Genomic DNA. Translation: AAB00323.1.
Z27116 Genomic DNA. Translation: CAA81631.1.
Z28305 Genomic DNA. Translation: CAA82159.1.
BK006944 Genomic DNA. Translation: DAA09230.1.
PIRiS31254.
RefSeqiNP_013006.3. NM_001179870.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EDZX-ray2.80A1-320[»]
1EE9X-ray3.00A1-320[»]
ProteinModelPortaliQ02046.
SMRiQ02046.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34211. 16 interactors.
DIPiDIP-730N.
IntActiQ02046. 1 interactor.
MINTiMINT-4493839.

Proteomic databases

MaxQBiQ02046.
PRIDEiQ02046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR080W; YKR080W; YKR080W.
GeneIDi853955.
KEGGisce:YKR080W.

Organism-specific databases

EuPathDBiFungiDB:YKR080W.
SGDiS000001788. MTD1.

Phylogenomic databases

HOGENOMiHOG000158212.
InParanoidiQ02046.
KOiK00295.
OMAiVYYPIFP.
OrthoDBiEOG092C3E8S.

Enzyme and pathway databases

BioCyciYEAST:YKR080W-MONOMER.
SABIO-RKQ02046.

Miscellaneous databases

EvolutionaryTraceiQ02046.
PROiQ02046.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTD1_YEAST
AccessioniPrimary (citable) accession number: Q02046
Secondary accession number(s): D6VXE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.