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Protein

Methylenetetrahydrofolate dehydrogenase [NAD(+)]

Gene

MTD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis.

Miscellaneous

Present with 16200 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei150By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 186NAD1 Publication2
Nucleotide bindingi208 – 209NAD1 Publication2
Nucleotide bindingi274 – 276NAD1 Publication3

GO - Molecular functioni

  • methylenetetrahydrofolate dehydrogenase (NAD+) activity Source: SGD

GO - Biological processi

  • folic acid-containing compound biosynthetic process Source: SGD
  • one-carbon metabolic process Source: SGD
  • purine nucleobase biosynthetic process Source: SGD
  • purine nucleotide biosynthetic process Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism, Purine biosynthesis
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YKR080W-MONOMER
SABIO-RKiQ02046

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate dehydrogenase [NAD(+)] (EC:1.5.1.15)
Gene namesi
Name:MTD1
Ordered Locus Names:YKR080W
ORF Names:YKR400
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR080W
SGDiS000001788 MTD1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001993191 – 320Methylenetetrahydrofolate dehydrogenase [NAD(+)]Add BLAST320

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiQ02046
PaxDbiQ02046
PRIDEiQ02046

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi34211, 34 interactors
DIPiDIP-730N
IntActiQ02046, 4 interactors
MINTiQ02046
STRINGi4932.YKR080W

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 31Combined sources22
Turni32 – 34Combined sources3
Beta strandi38 – 43Combined sources6
Helixi48 – 63Combined sources16
Beta strandi67 – 72Combined sources6
Helixi76 – 78Combined sources3
Helixi79 – 88Combined sources10
Beta strandi94 – 97Combined sources4
Beta strandi101 – 104Combined sources4
Helixi105 – 110Combined sources6
Turni111 – 113Combined sources3
Turni116 – 118Combined sources3
Beta strandi119 – 121Combined sources3
Helixi125 – 132Combined sources8
Beta strandi137 – 142Combined sources6
Helixi150 – 161Combined sources12
Turni174 – 177Combined sources4
Beta strandi179 – 183Combined sources5
Turni187 – 189Combined sources3
Helixi190 – 198Combined sources9
Beta strandi203 – 207Combined sources5
Beta strandi209 – 217Combined sources9
Beta strandi227 – 233Combined sources7
Helixi236 – 245Combined sources10
Beta strandi247 – 251Combined sources5
Turni262 – 264Combined sources3
Beta strandi269 – 273Combined sources5
Beta strandi275 – 277Combined sources3
Helixi282 – 285Combined sources4
Beta strandi288 – 293Combined sources6
Helixi296 – 316Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EDZX-ray2.80A1-320[»]
1EE9X-ray3.00A1-320[»]
ProteinModelPortaliQ02046
SMRiQ02046
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02046

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000158212
InParanoidiQ02046
KOiK00295
OMAiPCTPLAM
OrthoDBiEOG092C3E8S

Family and domain databases

CDDicd01079 NAD_bind_m-THF_DH, 1 hit
InterProiView protein in InterPro
IPR035812 m-THF_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Q02046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPGRTILA SKVAETFNTE IINNVEEYKK THNGQGPLLV GFLANNDPAA
60 70 80 90 100
KMYATWTQKT SESMGFRYDL RVIEDKDFLE EAIIQANGDD SVNGIMVYFP
110 120 130 140 150
VFGNAQDQYL QQVVCKEKDV EGLNHVYYQN LYHNVRYLDK ENRLKSILPC
160 170 180 190 200
TPLAIVKILE FLKIYNNLLP EGNRLYGKKC IVINRSEIVG RPLAALLAND
210 220 230 240 250
GATVYSVDVN NIQKFTRGES LKLNKHHVED LGEYSEDLLK KCSLDSDVVI
260 270 280 290 300
TGVPSENYKF PTEYIKEGAV CINFACTKNF SDDVKEKASL YVPMTGKVTI
310 320
AMLLRNMLRL VRNVELSKEK
Length:320
Mass (Da):36,240
Last modified:October 1, 1993 - v1
Checksum:iDA30D9E2CEB590C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02934 Genomic DNA Translation: AAB00323.1
Z27116 Genomic DNA Translation: CAA81631.1
Z28305 Genomic DNA Translation: CAA82159.1
BK006944 Genomic DNA Translation: DAA09230.1
PIRiS31254
RefSeqiNP_013006.3, NM_001179870.3

Genome annotation databases

EnsemblFungiiYKR080W; YKR080W; YKR080W
GeneIDi853955
KEGGisce:YKR080W

Similar proteinsi

Entry informationi

Entry nameiMTD1_YEAST
AccessioniPrimary (citable) accession number: Q02046
Secondary accession number(s): D6VXE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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