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Protein

Methylenetetrahydrofolate dehydrogenase [NAD(+)]

Gene

MTD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis.

Catalytic activityi

5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei150 – 1501By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi185 – 1862NAD1 Publication
Nucleotide bindingi208 – 2092NAD1 Publication
Nucleotide bindingi274 – 2763NAD1 Publication

GO - Molecular functioni

  • methylenetetrahydrofolate dehydrogenase (NAD+) activity Source: SGD

GO - Biological processi

  • folic acid-containing compound biosynthetic process Source: SGD
  • one-carbon metabolic process Source: SGD
  • purine nucleobase biosynthetic process Source: SGD
  • purine nucleotide biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YKR080W-MONOMER.
SABIO-RKQ02046.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate dehydrogenase [NAD(+)] (EC:1.5.1.15)
Gene namesi
Name:MTD1
Ordered Locus Names:YKR080W
ORF Names:YKR400
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR080W.
SGDiS000001788. MTD1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Methylenetetrahydrofolate dehydrogenase [NAD(+)]PRO_0000199319Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiQ02046.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi34211. 16 interactions.
DIPiDIP-730N.
IntActiQ02046. 1 interaction.
MINTiMINT-4493839.

Structurei

Secondary structure

1
320
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 3122Combined sources
Turni32 – 343Combined sources
Beta strandi38 – 436Combined sources
Helixi48 – 6316Combined sources
Beta strandi67 – 726Combined sources
Helixi76 – 783Combined sources
Helixi79 – 8810Combined sources
Beta strandi94 – 974Combined sources
Beta strandi101 – 1044Combined sources
Helixi105 – 1106Combined sources
Turni111 – 1133Combined sources
Turni116 – 1183Combined sources
Beta strandi119 – 1213Combined sources
Helixi125 – 1328Combined sources
Beta strandi137 – 1426Combined sources
Helixi150 – 16112Combined sources
Turni174 – 1774Combined sources
Beta strandi179 – 1835Combined sources
Turni187 – 1893Combined sources
Helixi190 – 1989Combined sources
Beta strandi203 – 2075Combined sources
Beta strandi209 – 2179Combined sources
Beta strandi227 – 2337Combined sources
Helixi236 – 24510Combined sources
Beta strandi247 – 2515Combined sources
Turni262 – 2643Combined sources
Beta strandi269 – 2735Combined sources
Beta strandi275 – 2773Combined sources
Helixi282 – 2854Combined sources
Beta strandi288 – 2936Combined sources
Helixi296 – 31621Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EDZX-ray2.80A1-320[»]
1EE9X-ray3.00A1-320[»]
ProteinModelPortaliQ02046.
SMRiQ02046. Positions 3-319.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02046.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000158212.
InParanoidiQ02046.
KOiK00295.
OMAiQIYNPIL.
OrthoDBiEOG7K6Q51.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPGRTILA SKVAETFNTE IINNVEEYKK THNGQGPLLV GFLANNDPAA
60 70 80 90 100
KMYATWTQKT SESMGFRYDL RVIEDKDFLE EAIIQANGDD SVNGIMVYFP
110 120 130 140 150
VFGNAQDQYL QQVVCKEKDV EGLNHVYYQN LYHNVRYLDK ENRLKSILPC
160 170 180 190 200
TPLAIVKILE FLKIYNNLLP EGNRLYGKKC IVINRSEIVG RPLAALLAND
210 220 230 240 250
GATVYSVDVN NIQKFTRGES LKLNKHHVED LGEYSEDLLK KCSLDSDVVI
260 270 280 290 300
TGVPSENYKF PTEYIKEGAV CINFACTKNF SDDVKEKASL YVPMTGKVTI
310 320
AMLLRNMLRL VRNVELSKEK
Length:320
Mass (Da):36,240
Last modified:October 1, 1993 - v1
Checksum:iDA30D9E2CEB590C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02934 Genomic DNA. Translation: AAB00323.1.
Z27116 Genomic DNA. Translation: CAA81631.1.
Z28305 Genomic DNA. Translation: CAA82159.1.
BK006944 Genomic DNA. Translation: DAA09230.1.
PIRiS31254.
RefSeqiNP_013006.3. NM_001179870.3.

Genome annotation databases

EnsemblFungiiYKR080W; YKR080W; YKR080W.
GeneIDi853955.
KEGGisce:YKR080W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02934 Genomic DNA. Translation: AAB00323.1.
Z27116 Genomic DNA. Translation: CAA81631.1.
Z28305 Genomic DNA. Translation: CAA82159.1.
BK006944 Genomic DNA. Translation: DAA09230.1.
PIRiS31254.
RefSeqiNP_013006.3. NM_001179870.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EDZX-ray2.80A1-320[»]
1EE9X-ray3.00A1-320[»]
ProteinModelPortaliQ02046.
SMRiQ02046. Positions 3-319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34211. 16 interactions.
DIPiDIP-730N.
IntActiQ02046. 1 interaction.
MINTiMINT-4493839.

Proteomic databases

MaxQBiQ02046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR080W; YKR080W; YKR080W.
GeneIDi853955.
KEGGisce:YKR080W.

Organism-specific databases

EuPathDBiFungiDB:YKR080W.
SGDiS000001788. MTD1.

Phylogenomic databases

HOGENOMiHOG000158212.
InParanoidiQ02046.
KOiK00295.
OMAiQIYNPIL.
OrthoDBiEOG7K6Q51.

Enzyme and pathway databases

BioCyciYEAST:YKR080W-MONOMER.
SABIO-RKQ02046.

Miscellaneous databases

EvolutionaryTraceiQ02046.
PROiQ02046.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the Saccharomyces cerevisiae gene encoding NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase."
    West M.G., Barlowe C.K., Appling D.R.
    J. Biol. Chem. 268:153-160(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 72-86 AND 146-160.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
    Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
    Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-161.
  5. "Isolation and characterization of a novel eukaryotic monofunctional NAD(+)-dependent 5,10-methylenetetrahydrofolate dehydrogenase."
    Barlowe C.K., Appling D.R.
    Biochemistry 29:7089-7094(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROTEIN.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The X-ray structure of the NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae."
    Monzingo A.F., Breksa A., Ernst S., Appling D.R., Robertus J.D.
    Protein Sci. 9:1374-1381(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NAD, SUBUNIT.

Entry informationi

Entry nameiMTD1_YEAST
AccessioniPrimary (citable) accession number: Q02046
Secondary accession number(s): D6VXE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 6, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.