Reviewed, UniProtKB/Swiss-Prot Q02046 (MTD1_YEAST)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate dehydrogenase [NAD+]
    EC=1.5.1.15

Gene names

Name:	MTD1
Ordered Locus Names:	YKR080W
ORF Names:	YKR400

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis.
Catalytic activity	5,10-methylenetetrahydrofolate + NAD(+) = 5,10-methenyltetrahydrofolate + NADH.
Cofactor	NAD.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm. Nucleus.
Post-translational modification	The N-terminus is blocked.
Miscellaneous	Present with 16200 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	One-carbon metabolism Purine biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base biosynthetic process Inferred from direct assay. Source: SGD purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro methylenetetrahydrofolate dehydrogenase (NAD+) activity Ref.1 Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 320

320

Methylenetetrahydrofolate dehydrogenase [NAD+]

PRO_0000199319

Regions

Nucleotide binding

185 – 186

2

NAD

Nucleotide binding

208 – 209

2

NAD

Nucleotide binding

274 – 276

3

NAD

Sites

Active site

150

1

By similarity

Secondary structure

1

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320

Helix Strand Turn

Details...

Helix

10 – 31

22

Turn

32 – 34

3

Beta strand

38 – 43

6

Helix

48 – 63

16

Beta strand

67 – 72

6

Helix

76 – 78

3

Helix

79 – 88

10

Beta strand

94 – 97

4

Beta strand

101 – 104

4

Helix

105 – 110

6

Turn

111 – 113

3

Turn

116 – 118

3

Helix

125 – 132

8

Beta strand

137 – 142

6

Helix

150 – 161

12

Turn

174 – 177

4

Beta strand

179 – 183

5

Turn

187 – 189

3

Helix

190 – 198

9

Beta strand

203 – 207

5

Beta strand

209 – 217

9

Beta strand

227 – 233

7

Helix

236 – 245

10

Beta strand

247 – 251

5

Turn

262 – 264

3

Beta strand

269 – 273

5

Beta strand

275 – 277

3

Helix

282 – 285

4

Beta strand

288 – 293

6

Helix

296 – 316

21

Sequences

	Sequence		Length	Mass (Da)
	Q02046-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: DA30D9E2CEB590C6	FASTA	320	36,240
	10 20 30 40 50 60 MSKPGRTILA SKVAETFNTE IINNVEEYKK THNGQGPLLV GFLANNDPAA KMYATWTQKT 70 80 90 100 110 120 SESMGFRYDL RVIEDKDFLE EAIIQANGDD SVNGIMVYFP VFGNAQDQYL QQVVCKEKDV 130 140 150 160 170 180 EGLNHVYYQN LYHNVRYLDK ENRLKSILPC TPLAIVKILE FLKIYNNLLP EGNRLYGKKC 190 200 210 220 230 240 IVINRSEIVG RPLAALLAND GATVYSVDVN NIQKFTRGES LKLNKHHVED LGEYSEDLLK 250 260 270 280 290 300 KCSLDSDVVI TGVPSENYKF PTEYIKEGAV CINFACTKNF SDDVKEKASL YVPMTGKVTI 310 320 AMLLRNMLRL VRNVELSKEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Saccharomyces cerevisiae gene encoding NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase." West M.G., Barlowe C.K., Appling D.R. J. Biol. Chem. 268:153-160(1993) [PubMed: 8416923] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 72-86 AND 146-160.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames." Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F. Yeast 10:231-245(1994) [PubMed: 8203164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-161.
[4]	"Isolation and characterization of a novel eukaryotic monofunctional NAD(+)-dependent 5,10-methylenetetrahydrofolate dehydrogenase." Barlowe C.K., Appling D.R. Biochemistry 29:7089-7094(1990) [PubMed: 2223762] [Abstract] Cited for: IDENTIFICATION OF PROTEIN.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The X-ray structure of the NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae." Monzingo A.F., Breksa A., Ernst S., Appling D.R., Robertus J.D. Protein Sci. 9:1374-1381(2000) [PubMed: 10933503] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NAD, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L02934 Genomic DNA. Translation: AAB00323.1.
Z27116 Genomic DNA. Translation: CAA81631.1.
Z28305 Genomic DNA. Translation: CAA82159.1.

PIR

S31254.

RefSeq

NP_013006.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EDZ	X-ray	2.80	A	1-320	[»]
1EE9	X-ray	3.00	A	1-320	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:730N.

Proteomic databases

PeptideAtlas

Q02046.

Genome annotation databases

Ensembl

YKR080W. Saccharomyces cerevisiae. [Contig view]

GeneID

853955.

GenomeReviews

Gene locus YKR080W in contig Y13137_GR.

KEGG

sce:YKR080W.

NMPDR

fig|4932.3.peg.3993.

Organism-specific databases

CYGD

YKR080w.

SGD

S000001788. MTD1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

Q02046.

Gene expression databases

ArrayExpress

Q02046.

GermOnline

YKR080W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016040. NAD(P)-bd.
IPR000672. THF_DHase/CycOHase.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]

PRINTS

PR00085. THFDHDRGNASE.

ProDom

PD002300. THFDhg/Cyc_hydro. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other Resources

LinkHub

Q02046.

NextBio

975368.

Entry information

Entry name

MTD1_YEAST

Accession

Primary (citable) accession number: Q02046

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 25, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)