ID   MYL5_HUMAN              Reviewed;         173 AA.
AC   Q02045; Q8IXL8;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   24-JAN-2024, entry version 176.
DE   RecName: Full=Myosin light chain 5;
DE   AltName: Full=Myosin regulatory light chain 5;
DE   AltName: Full=Superfast myosin regulatory light chain 2;
DE            Short=MYLC2;
DE            Short=MyLC-2;
GN   Name=MYL5;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=1284596;
RA   Collins C.C., Schappert K.T., Hayden M.R.;
RT   "The genomic organization of a novel regulatory myosin light chain gene
RT   (MYL5) that maps to chromosome 4p16.3 and shows different patterns of
RT   expression between primates.";
RL   Hum. Mol. Genet. 1:727-733(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q02045-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q02045-2; Sequence=VSP_026448;
CC   -!- TISSUE SPECIFICITY: Expressed in fetal skeletal muscle and retina.
CC   -!- MISCELLANEOUS: This chain binds calcium. {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L03785; AAA59890.1; -; mRNA.
DR   EMBL; BC040050; AAH40050.1; -; mRNA.
DR   CCDS; CCDS43197.1; -. [Q02045-1]
DR   CCDS; CCDS87196.1; -. [Q02045-2]
DR   PIR; I54328; I54328.
DR   RefSeq; NP_002468.1; NM_002477.1. [Q02045-1]
DR   RefSeq; XP_016863734.1; XM_017008245.1.
DR   RefSeq; XP_016863735.1; XM_017008246.1.
DR   RefSeq; XP_016863736.1; XM_017008247.1.
DR   RefSeq; XP_016863737.1; XM_017008248.1.
DR   AlphaFoldDB; Q02045; -.
DR   SMR; Q02045; -.
DR   BioGRID; 110720; 5.
DR   IntAct; Q02045; 2.
DR   STRING; 9606.ENSP00000383023; -.
DR   iPTMnet; Q02045; -.
DR   PhosphoSitePlus; Q02045; -.
DR   BioMuta; MYL5; -.
DR   DMDM; 400266; -.
DR   MassIVE; Q02045; -.
DR   PaxDb; 9606-ENSP00000383023; -.
DR   PeptideAtlas; Q02045; -.
DR   ProteomicsDB; 58033; -. [Q02045-1]
DR   ProteomicsDB; 58034; -. [Q02045-2]
DR   Antibodypedia; 22136; 79 antibodies from 23 providers.
DR   DNASU; 4636; -.
DR   Ensembl; ENST00000400159.7; ENSP00000383023.2; ENSG00000215375.7. [Q02045-1]
DR   Ensembl; ENST00000505477.5; ENSP00000423118.1; ENSG00000215375.7. [Q02045-2]
DR   Ensembl; ENST00000506838.5; ENSP00000427153.1; ENSG00000215375.7. [Q02045-2]
DR   Ensembl; ENST00000511290.5; ENSP00000425162.1; ENSG00000215375.7. [Q02045-2]
DR   GeneID; 4636; -.
DR   KEGG; hsa:4636; -.
DR   MANE-Select; ENST00000400159.7; ENSP00000383023.2; NM_002477.2; NP_002468.1.
DR   UCSC; uc003gav.4; human. [Q02045-1]
DR   AGR; HGNC:7586; -.
DR   CTD; 4636; -.
DR   DisGeNET; 4636; -.
DR   GeneCards; MYL5; -.
DR   HGNC; HGNC:7586; MYL5.
DR   HPA; ENSG00000215375; Low tissue specificity.
DR   MIM; 160782; gene.
DR   neXtProt; NX_Q02045; -.
DR   OpenTargets; ENSG00000215375; -.
DR   PharmGKB; PA31383; -.
DR   VEuPathDB; HostDB:ENSG00000215375; -.
DR   eggNOG; KOG0031; Eukaryota.
DR   GeneTree; ENSGT00940000163023; -.
DR   HOGENOM; CLU_061288_9_4_1; -.
DR   InParanoid; Q02045; -.
DR   OMA; RLMMSQG; -.
DR   OrthoDB; 312751at2759; -.
DR   PhylomeDB; Q02045; -.
DR   TreeFam; TF314218; -.
DR   PathwayCommons; Q02045; -.
DR   Reactome; R-HSA-445355; Smooth Muscle Contraction.
DR   SignaLink; Q02045; -.
DR   BioGRID-ORCS; 4636; 12 hits in 1155 CRISPR screens.
DR   ChiTaRS; MYL5; human.
DR   GenomeRNAi; 4636; -.
DR   Pharos; Q02045; Tbio.
DR   PRO; PR:Q02045; -.
DR   Proteomes; UP000005640; Chromosome 4.
DR   RNAct; Q02045; Protein.
DR   Bgee; ENSG00000215375; Expressed in mucosa of transverse colon and 141 other cell types or tissues.
DR   ExpressionAtlas; Q02045; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0005859; C:muscle myosin complex; TAS:ProtInc.
DR   GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR   GO; GO:0008307; F:structural constituent of muscle; TAS:ProtInc.
DR   GO; GO:0006937; P:regulation of muscle contraction; TAS:ProtInc.
DR   Gene3D; 1.10.238.10; EF-hand; 2.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   PANTHER; PTHR23049:SF8; MYOSIN LIGHT CHAIN 5; 1.
DR   PANTHER; PTHR23049; MYOSIN REGULATORY LIGHT CHAIN 2; 1.
DR   Pfam; PF13499; EF-hand_7; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; EF-hand; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 3.
DR   Genevisible; Q02045; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Calcium; Metal-binding; Motor protein;
KW   Muscle protein; Myosin; Reference proteome; Repeat.
FT   CHAIN           1..173
FT                   /note="Myosin light chain 5"
FT                   /id="PRO_0000198745"
FT   DOMAIN          30..65
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          100..135
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          136..171
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         43
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         45
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         47
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         54
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   VAR_SEQ         1..41
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_026448"
FT   VARIANT         88
FT                   /note="F -> S (in dbSNP:rs2228354)"
FT                   /id="VAR_050459"
SQ   SEQUENCE   173 AA;  19534 MW;  79615F658A68157F CRC64;
     MASRKTKKKE GGALRAQRAS SNVFSNFEQT QIQEFKEAFT LMDQNRDGFI DKEDLKDTYA
     SLGKTNVKDD ELDAMLKEAS GPINFTMFLN LFGEKLSGTD AEETILNAFK MLDPDGKGKI
     NKEYIKRLLM SQADKMTAEE VDQMFQFASI DVAGNLDYKA LSYVITHGEE KEE
//