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Protein

Aquaporin-1

Gene

Aqp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei56 – 561Substrate discriminationBy similarity
Sitei180 – 1801Substrate discriminationBy similarity
Sitei189 – 1891Hg(2+)-sensitive residueBy similarity
Sitei195 – 1951Substrate discriminationBy similarity

GO - Molecular functioni

  1. ammonium transmembrane transporter activity Source: UniProtKB
  2. carbon dioxide transmembrane transporter activity Source: UniProtKB
  3. ephrin receptor binding Source: UniProtKB
  4. glycerol channel activity Source: GO_Central
  5. glycerol transmembrane transporter activity Source: UniProtKB
  6. intracellular cGMP activated cation channel activity Source: UniProtKB
  7. nitric oxide transmembrane transporter activity Source: UniProtKB
  8. potassium channel activity Source: UniProtKB
  9. potassium ion transmembrane transporter activity Source: UniProtKB
  10. transmembrane transporter activity Source: MGI
  11. water channel activity Source: UniProtKB
  12. water transmembrane transporter activity Source: MGI

GO - Biological processi

  1. ammonium transmembrane transport Source: MGI
  2. ammonium transport Source: UniProtKB
  3. camera-type eye morphogenesis Source: MGI
  4. carbon dioxide transmembrane transport Source: UniProtKB
  5. carbon dioxide transport Source: UniProtKB
  6. cation transmembrane transport Source: MGI
  7. cellular homeostasis Source: UniProtKB
  8. cellular hyperosmotic response Source: UniProtKB
  9. cellular response to cAMP Source: UniProtKB
  10. cellular response to copper ion Source: UniProtKB
  11. cellular response to dexamethasone stimulus Source: UniProtKB
  12. cellular response to hydrogen peroxide Source: UniProtKB
  13. cellular response to hypoxia Source: UniProtKB
  14. cellular response to inorganic substance Source: UniProtKB
  15. cellular response to mechanical stimulus Source: UniProtKB
  16. cellular response to mercury ion Source: UniProtKB
  17. cellular response to nitric oxide Source: MGI
  18. cellular response to retinoic acid Source: UniProtKB
  19. cellular response to salt stress Source: UniProtKB
  20. cellular response to stress Source: UniProtKB
  21. cellular response to UV Source: UniProtKB
  22. cellular water homeostasis Source: GO_Central
  23. cell volume homeostasis Source: UniProtKB
  24. cerebrospinal fluid secretion Source: Ensembl
  25. cGMP biosynthetic process Source: UniProtKB
  26. corticotropin secretion Source: MGI
  27. establishment or maintenance of actin cytoskeleton polarity Source: UniProtKB
  28. glomerular filtration Source: MGI
  29. glycerol transport Source: UniProtKB
  30. hyperosmotic salinity response Source: Ensembl
  31. lateral ventricle development Source: UniProtKB
  32. lipid digestion Source: MGI
  33. maintenance of symbiont-containing vacuole by host Source: UniProtKB
  34. metanephric descending thin limb development Source: UniProtKB
  35. metanephric glomerulus vasculature development Source: UniProtKB
  36. metanephric proximal convoluted tubule segment 2 development Source: UniProtKB
  37. metanephric proximal straight tubule development Source: UniProtKB
  38. negative regulation of apoptotic process Source: UniProtKB
  39. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  40. nitric oxide transport Source: UniProtKB
  41. odontogenesis Source: Ensembl
  42. pancreatic juice secretion Source: Ensembl
  43. positive regulation of angiogenesis Source: UniProtKB
  44. positive regulation of cell migration Source: MGI
  45. positive regulation of epithelial cell migration Source: Ensembl
  46. positive regulation of fibroblast proliferation Source: UniProtKB
  47. positive regulation of lamellipodium assembly Source: Ensembl
  48. positive regulation of saliva secretion Source: UniProtKB
  49. potassium ion transmembrane transport Source: MGI
  50. potassium ion transport Source: UniProtKB
  51. renal water absorption Source: MGI
  52. renal water transport Source: MGI
  53. response to drug Source: UniProtKB
  54. response to estrogen Source: Ensembl
  55. secretion by cell Source: MGI
  56. secretory granule organization Source: MGI
  57. sensory perception of pain Source: MGI
  58. transepithelial water transport Source: UniProtKB
  59. water homeostasis Source: MGI
  60. water transport Source: MGI
  61. wound healing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_309678. Erythrocytes take up oxygen and release carbon dioxide.
REACT_318918. Erythrocytes take up carbon dioxide and release oxygen.
REACT_327080. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-1
Short name:
AQP-1
Alternative name(s):
Aquaporin-CHIP
Delayed early response protein 2
Short name:
DER2
Water channel protein for red blood cells and kidney proximal tubule
Gene namesi
Name:Aqp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:103201. Aqp1.

Subcellular locationi

  1. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 76CytoplasmicBy similarity
Transmembranei8 – 3629Helical; Name=Helix 1By similarityAdd
BLAST
Topological domaini37 – 4812ExtracellularBy similarityAdd
BLAST
Transmembranei49 – 6618Helical; Name=Helix 2By similarityAdd
BLAST
Topological domaini67 – 704CytoplasmicBy similarity
Intramembranei71 – 766By similarity
Intramembranei77 – 848Helical; Name=Helix BBy similarity
Topological domaini85 – 9410CytoplasmicBy similarity
Transmembranei95 – 11521Helical; Name=Helix 3By similarityAdd
BLAST
Topological domaini116 – 13621ExtracellularBy similarityAdd
BLAST
Transmembranei137 – 15519Helical; Name=Helix 4By similarityAdd
BLAST
Topological domaini156 – 16611CytoplasmicBy similarityAdd
BLAST
Transmembranei167 – 18317Helical; Name=Helix 5By similarityAdd
BLAST
Topological domaini184 – 1863ExtracellularBy similarity
Intramembranei187 – 1926By similarity
Intramembranei193 – 2008Helical; Name=Helix EBy similarity
Topological domaini201 – 2077ExtracellularBy similarity
Transmembranei208 – 22821Helical; Name=Helix 6By similarityAdd
BLAST
Topological domaini229 – 26941CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. apical part of cell Source: MGI
  2. apical plasma membrane Source: UniProtKB
  3. axon Source: MGI
  4. axon terminus Source: Ensembl
  5. basal plasma membrane Source: UniProtKB
  6. basolateral plasma membrane Source: UniProtKB
  7. brush border Source: UniProtKB
  8. brush border membrane Source: UniProtKB
  9. cytoplasm Source: UniProtKB
  10. dense core granule membrane Source: MGI
  11. extracellular vesicular exosome Source: MGI
  12. integral component of membrane Source: MGI
  13. integral component of plasma membrane Source: MGI
  14. membrane Source: MGI
  15. neuronal cell body membrane Source: Ensembl
  16. nuclear membrane Source: UniProtKB
  17. nucleus Source: UniProtKB
  18. plasma membrane Source: MGI
  19. sarcolemma Source: UniProtKB
  20. symbiont-containing vacuole Source: UniProtKB
  21. symbiont-containing vacuole membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice are born at the expected Mendelian rate and appear healthy and normal, but display strongly reduced urine osmolality. Besides, their erythrocytes show reduced water permeability. Mice lacking both Aqp1 and Slc14a1 are born at the expected Mendelian ratio, but do not thrive; half of them die within ten days after birth and none are alive after two weeks. Urine osmolality is somewhat lower than that observed with mice lacking only Aqp1. Besides, erythrocyte water permeability is significantly lower than in mice lacking only Aqp1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 269268Aquaporin-1PRO_0000063921Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis
Modified residuei262 – 2621Phosphoserine1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ02013.
PaxDbiQ02013.
PRIDEiQ02013.

PTM databases

PhosphoSiteiQ02013.

Expressioni

Tissue specificityi

Detected in erythrocytes (at protein level). Erythrocytes and renal tubules.1 Publication

Gene expression databases

BgeeiQ02013.
CleanExiMM_AQP1.
GenevestigatoriQ02013.

Interactioni

Subunit structurei

Homotetramer. Identified in a complex with STOM (By similarity). Interacts with EPHB2; involved in endolymph production in the inner ear.By similarity1 Publication

Protein-protein interaction databases

BioGridi198170. 1 interaction.
IntActiQ02013. 2 interactions.
MINTiMINT-4088080.

Structurei

3D structure databases

ProteinModelPortaliQ02013.
SMRiQ02013. Positions 9-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 783NPA 1
Motifi192 – 1943NPA 2

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi159 – 1624Poly-Arg

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0580.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiQ02013.
KOiK09864.
OMAiFNNHWIF.
OrthoDBiEOG7N8ZWD.
PhylomeDBiQ02013.
TreeFamiTF312940.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023274. Aquaporin_1.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02013. AQUAPORIN1.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEIKKKLF WRAVVAEFLA MTLFVFISIG SALGFNYPLE RNQTLVQDNV
60 70 80 90 100
KVSLAFGLSI ATLAQSVGHI SGAHLNPAVT LGLLLSCQIS ILRAVMYIIA
110 120 130 140 150
QCVGAIVATA ILSGITSSLV DNSLGRNDLA HGVNSGQGLG IEIIGTLQLV
160 170 180 190 200
LCVLATTDRR RRDLGGSAPL AIGLSVALGH LLAIDYTGCG INPARSFGSA
210 220 230 240 250
VLTRNFSNHW IFWVGPFIGG ALAVLIYDFI LAPRSSDFTD RMKVWTSGQV
260
EEYDLDADDI NSRVEMKPK
Length:269
Mass (Da):28,793
Last modified:January 23, 2007 - v3
Checksum:iF0499724AD4AB5F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901G → S in AAH07125 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02914 mRNA. Translation: AAB53928.1.
AK081886 mRNA. Translation: BAC38360.1.
AK086688 mRNA. Translation: BAC39719.1.
AK157333 mRNA. Translation: BAE34051.1.
AK158226 mRNA. Translation: BAE34412.1.
AK158389 mRNA. Translation: BAE34482.1.
AK171627 mRNA. Translation: BAE42573.1.
AK172361 mRNA. Translation: BAE42966.1.
BC007125 mRNA. Translation: AAH07125.1.
CCDSiCCDS20164.1.
PIRiB44499.
RefSeqiNP_031498.1. NM_007472.2.
UniGeneiMm.18625.

Genome annotation databases

EnsembliENSMUST00000004774; ENSMUSP00000004774; ENSMUSG00000004655.
GeneIDi11826.
KEGGimmu:11826.
UCSCiuc009caq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02914 mRNA. Translation: AAB53928.1.
AK081886 mRNA. Translation: BAC38360.1.
AK086688 mRNA. Translation: BAC39719.1.
AK157333 mRNA. Translation: BAE34051.1.
AK158226 mRNA. Translation: BAE34412.1.
AK158389 mRNA. Translation: BAE34482.1.
AK171627 mRNA. Translation: BAE42573.1.
AK172361 mRNA. Translation: BAE42966.1.
BC007125 mRNA. Translation: AAH07125.1.
CCDSiCCDS20164.1.
PIRiB44499.
RefSeqiNP_031498.1. NM_007472.2.
UniGeneiMm.18625.

3D structure databases

ProteinModelPortaliQ02013.
SMRiQ02013. Positions 9-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198170. 1 interaction.
IntActiQ02013. 2 interactions.
MINTiMINT-4088080.

PTM databases

PhosphoSiteiQ02013.

Proteomic databases

MaxQBiQ02013.
PaxDbiQ02013.
PRIDEiQ02013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004774; ENSMUSP00000004774; ENSMUSG00000004655.
GeneIDi11826.
KEGGimmu:11826.
UCSCiuc009caq.1. mouse.

Organism-specific databases

CTDi358.
MGIiMGI:103201. Aqp1.

Phylogenomic databases

eggNOGiCOG0580.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiQ02013.
KOiK09864.
OMAiFNNHWIF.
OrthoDBiEOG7N8ZWD.
PhylomeDBiQ02013.
TreeFamiTF312940.

Enzyme and pathway databases

ReactomeiREACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_309678. Erythrocytes take up oxygen and release carbon dioxide.
REACT_318918. Erythrocytes take up carbon dioxide and release oxygen.
REACT_327080. Passive transport by Aquaporins.

Miscellaneous databases

NextBioi279727.
PROiQ02013.
SOURCEiSearch...

Gene expression databases

BgeeiQ02013.
CleanExiMM_AQP1.
GenevestigatoriQ02013.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023274. Aquaporin_1.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02013. AQUAPORIN1.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Growth factor-induced delayed early response genes."
    Lanahan A.A., Williams J.B., Sanders L.K., Nathans D.
    Mol. Cell. Biol. 12:3919-3929(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Head, Inner ear and Spleen.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. "EphB2 guides axons at the midline and is necessary for normal vestibular function."
    Cowan C.A., Yokoyama N., Bianchi L.M., Henkemeyer M., Fritzsch B.
    Neuron 26:417-430(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EPHB2.
  5. "Analysis of double knockout mice lacking aquaporin-1 and urea transporter UT-B. Evidence for UT-B-facilitated water transport in erythrocytes."
    Yang B., Verkman A.S.
    J. Biol. Chem. 277:36782-36786(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiAQP1_MOUSE
AccessioniPrimary (citable) accession number: Q02013
Secondary accession number(s): Q542P1, Q91VY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pharmacologically inhibited by submillimolar concentrations of mercury.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.