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Protein

Aquaporin-1

Gene

Aqp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Substrate discriminationBy similarity1
Sitei180Substrate discriminationBy similarity1
Sitei189Hg(2+)-sensitive residueBy similarity1
Sitei195Substrate discriminationBy similarity1

GO - Molecular functioni

GO - Biological processi

  • ammonium transmembrane transport Source: GO_Central
  • ammonium transport Source: UniProtKB
  • camera-type eye morphogenesis Source: MGI
  • carbon dioxide transmembrane transport Source: UniProtKB
  • carbon dioxide transport Source: UniProtKB
  • cellular homeostasis Source: UniProtKB
  • cellular hyperosmotic response Source: UniProtKB
  • cellular response to cAMP Source: UniProtKB
  • cellular response to copper ion Source: UniProtKB
  • cellular response to dexamethasone stimulus Source: UniProtKB
  • cellular response to hydrogen peroxide Source: UniProtKB
  • cellular response to hypoxia Source: UniProtKB
  • cellular response to inorganic substance Source: UniProtKB
  • cellular response to mechanical stimulus Source: UniProtKB
  • cellular response to mercury ion Source: UniProtKB
  • cellular response to nitric oxide Source: MGI
  • cellular response to retinoic acid Source: UniProtKB
  • cellular response to salt stress Source: UniProtKB
  • cellular response to stress Source: UniProtKB
  • cellular response to UV Source: UniProtKB
  • cellular water homeostasis Source: GO_Central
  • cell volume homeostasis Source: UniProtKB
  • cGMP biosynthetic process Source: UniProtKB
  • corticotropin secretion Source: MGI
  • establishment or maintenance of actin cytoskeleton polarity Source: UniProtKB
  • glomerular filtration Source: MGI
  • glycerol transport Source: UniProtKB
  • hyperosmotic salinity response Source: Ensembl
  • lateral ventricle development Source: UniProtKB
  • lipid digestion Source: MGI
  • maintenance of symbiont-containing vacuole by host Source: UniProtKB
  • metanephric descending thin limb development Source: UniProtKB
  • metanephric glomerulus vasculature development Source: UniProtKB
  • metanephric proximal convoluted tubule segment 2 development Source: UniProtKB
  • metanephric proximal straight tubule development Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • nitric oxide transport Source: UniProtKB
  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of cell migration Source: MGI
  • positive regulation of epithelial cell migration Source: Ensembl
  • positive regulation of fibroblast proliferation Source: UniProtKB
  • positive regulation of lamellipodium assembly Source: Ensembl
  • positive regulation of saliva secretion Source: UniProtKB
  • potassium ion transport Source: UniProtKB
  • renal water absorption Source: MGI
  • renal water transport Source: MGI
  • response to drug Source: UniProtKB
  • response to estrogen Source: Ensembl
  • secretion by cell Source: MGI
  • secretory granule organization Source: MGI
  • sensory perception of pain Source: MGI
  • transepithelial water transport Source: UniProtKB
  • water homeostasis Source: MGI
  • water transport Source: MGI
  • wound healing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-MMU-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-MMU-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-MMU-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-1
Short name:
AQP-1
Alternative name(s):
Aquaporin-CHIP
Delayed early response protein 2
Short name:
DER2
Water channel protein for red blood cells and kidney proximal tubule
Gene namesi
Name:Aqp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:103201. Aqp1.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 7CytoplasmicBy similarity6
Transmembranei8 – 36Helical; Name=Helix 1By similarityAdd BLAST29
Topological domaini37 – 48ExtracellularBy similarityAdd BLAST12
Transmembranei49 – 66Helical; Name=Helix 2By similarityAdd BLAST18
Topological domaini67 – 70CytoplasmicBy similarity4
Intramembranei71 – 76By similarity6
Intramembranei77 – 84Helical; Name=Helix BBy similarity8
Topological domaini85 – 94CytoplasmicBy similarity10
Transmembranei95 – 115Helical; Name=Helix 3By similarityAdd BLAST21
Topological domaini116 – 136ExtracellularBy similarityAdd BLAST21
Transmembranei137 – 155Helical; Name=Helix 4By similarityAdd BLAST19
Topological domaini156 – 166CytoplasmicBy similarityAdd BLAST11
Transmembranei167 – 183Helical; Name=Helix 5By similarityAdd BLAST17
Topological domaini184 – 186ExtracellularBy similarity3
Intramembranei187 – 192By similarity6
Intramembranei193 – 200Helical; Name=Helix EBy similarity8
Topological domaini201 – 207ExtracellularBy similarity7
Transmembranei208 – 228Helical; Name=Helix 6By similarityAdd BLAST21
Topological domaini229 – 269CytoplasmicBy similarityAdd BLAST41

GO - Cellular componenti

  • apical part of cell Source: MGI
  • apical plasma membrane Source: UniProtKB
  • axon Source: MGI
  • axon terminus Source: Ensembl
  • basal plasma membrane Source: UniProtKB
  • basolateral plasma membrane Source: UniProtKB
  • brush border Source: UniProtKB
  • brush border membrane Source: UniProtKB
  • caveola Source: BHF-UCL
  • cytoplasm Source: UniProtKB
  • dense core granule membrane Source: MGI
  • extracellular exosome Source: MGI
  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: MGI
  • membrane Source: MGI
  • neuronal cell body membrane Source: Ensembl
  • nuclear membrane Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: MGI
  • sarcolemma Source: UniProtKB
  • symbiont-containing vacuole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice are born at the expected Mendelian rate and appear healthy and normal, but display strongly reduced urine osmolality. Besides, their erythrocytes show reduced water permeability. Mice lacking both Aqp1 and Slc14a1 are born at the expected Mendelian ratio, but do not thrive; half of them die within ten days after birth and none are alive after two weeks. Urine osmolality is somewhat lower than that observed with mice lacking only Aqp1. Besides, erythrocyte water permeability is significantly lower than in mice lacking only Aqp1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000639212 – 269Aquaporin-1Add BLAST268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi205N-linked (GlcNAc...)Sequence analysis1
Modified residuei247PhosphoserineCombined sources1
Modified residuei253PhosphotyrosineCombined sources1
Modified residuei262PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ02013.
PaxDbiQ02013.
PeptideAtlasiQ02013.
PRIDEiQ02013.

PTM databases

iPTMnetiQ02013.
PhosphoSitePlusiQ02013.
SwissPalmiQ02013.

Expressioni

Tissue specificityi

Detected in erythrocytes (at protein level). Erythrocytes and renal tubules.1 Publication

Gene expression databases

BgeeiENSMUSG00000004655.
CleanExiMM_AQP1.
GenevisibleiQ02013. MM.

Interactioni

Subunit structurei

Homotetramer. Identified in a complex with STOM (By similarity). Interacts with EPHB2; involved in endolymph production in the inner ear.By similarity1 Publication

GO - Molecular functioni

  • ephrin receptor binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ02013. 2 interactors.
MINTiMINT-4088080.
STRINGi10090.ENSMUSP00000004774.

Structurei

3D structure databases

ProteinModelPortaliQ02013.
SMRiQ02013.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 78NPA 13
Motifi192 – 194NPA 23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi159 – 162Poly-Arg4

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiQ02013.
KOiK09864.
OMAiMLICKLA.
OrthoDBiEOG091G166T.
PhylomeDBiQ02013.
TreeFamiTF312940.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023274. Aquaporin_1.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02013. AQUAPORIN1.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEIKKKLF WRAVVAEFLA MTLFVFISIG SALGFNYPLE RNQTLVQDNV
60 70 80 90 100
KVSLAFGLSI ATLAQSVGHI SGAHLNPAVT LGLLLSCQIS ILRAVMYIIA
110 120 130 140 150
QCVGAIVATA ILSGITSSLV DNSLGRNDLA HGVNSGQGLG IEIIGTLQLV
160 170 180 190 200
LCVLATTDRR RRDLGGSAPL AIGLSVALGH LLAIDYTGCG INPARSFGSA
210 220 230 240 250
VLTRNFSNHW IFWVGPFIGG ALAVLIYDFI LAPRSSDFTD RMKVWTSGQV
260
EEYDLDADDI NSRVEMKPK
Length:269
Mass (Da):28,793
Last modified:January 23, 2007 - v3
Checksum:iF0499724AD4AB5F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti190G → S in AAH07125 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02914 mRNA. Translation: AAB53928.1.
AK081886 mRNA. Translation: BAC38360.1.
AK086688 mRNA. Translation: BAC39719.1.
AK157333 mRNA. Translation: BAE34051.1.
AK158226 mRNA. Translation: BAE34412.1.
AK158389 mRNA. Translation: BAE34482.1.
AK171627 mRNA. Translation: BAE42573.1.
AK172361 mRNA. Translation: BAE42966.1.
BC007125 mRNA. Translation: AAH07125.1.
CCDSiCCDS20164.1.
PIRiB44499.
RefSeqiNP_031498.1. NM_007472.2.
UniGeneiMm.18625.

Genome annotation databases

EnsembliENSMUST00000004774; ENSMUSP00000004774; ENSMUSG00000004655.
GeneIDi11826.
KEGGimmu:11826.
UCSCiuc009caq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02914 mRNA. Translation: AAB53928.1.
AK081886 mRNA. Translation: BAC38360.1.
AK086688 mRNA. Translation: BAC39719.1.
AK157333 mRNA. Translation: BAE34051.1.
AK158226 mRNA. Translation: BAE34412.1.
AK158389 mRNA. Translation: BAE34482.1.
AK171627 mRNA. Translation: BAE42573.1.
AK172361 mRNA. Translation: BAE42966.1.
BC007125 mRNA. Translation: AAH07125.1.
CCDSiCCDS20164.1.
PIRiB44499.
RefSeqiNP_031498.1. NM_007472.2.
UniGeneiMm.18625.

3D structure databases

ProteinModelPortaliQ02013.
SMRiQ02013.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ02013. 2 interactors.
MINTiMINT-4088080.
STRINGi10090.ENSMUSP00000004774.

PTM databases

iPTMnetiQ02013.
PhosphoSitePlusiQ02013.
SwissPalmiQ02013.

Proteomic databases

MaxQBiQ02013.
PaxDbiQ02013.
PeptideAtlasiQ02013.
PRIDEiQ02013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004774; ENSMUSP00000004774; ENSMUSG00000004655.
GeneIDi11826.
KEGGimmu:11826.
UCSCiuc009caq.1. mouse.

Organism-specific databases

CTDi358.
MGIiMGI:103201. Aqp1.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiQ02013.
KOiK09864.
OMAiMLICKLA.
OrthoDBiEOG091G166T.
PhylomeDBiQ02013.
TreeFamiTF312940.

Enzyme and pathway databases

ReactomeiR-MMU-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-MMU-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-MMU-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-MMU-432047. Passive transport by Aquaporins.

Miscellaneous databases

PROiQ02013.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004655.
CleanExiMM_AQP1.
GenevisibleiQ02013. MM.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023274. Aquaporin_1.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02013. AQUAPORIN1.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAQP1_MOUSE
AccessioniPrimary (citable) accession number: Q02013
Secondary accession number(s): Q542P1, Q91VY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pharmacologically inhibited by submillimolar concentrations of mercury.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.