Q01V13 (GLPK_SOLUE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Acid_5555

Organism

Solibacter usitatus (strain Ellin6076) [Complete proteome] [HAMAP]

Taxonomic identifier

234267 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Solibacteres › Solibacterales › Solibacteraceae › Candidatus Solibacter

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Glycerol kinase HAMAP MF_00186		PRO_1000020789
Regions
	Nucleotide binding	408 – 412	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity
	Binding site	263	1	ATP By similarity
	Binding site	307	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01V13 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: A4277307549472BF	FASTA	494	54,310
	10 20 30 40 50 60 MSNYIGAIDQ GTTSTRFIVF DHGGRIVSVA QKEHEQIFPQ PGWVEHDANE IWRRTREVIA 70 80 90 100 110 120 EALEKRALSA SDLAAIGITN QRETTVVWDR LSGEPVYNAL VWQDTRTAAA VAELARDGGA 130 140 150 160 170 180 DRFRAQTGLP LATYFSALKI GWILDNVPAV RARAEAGDIL FGNIDTFLLW NLTGGLHLTD 190 200 210 220 230 240 CTNASRTHLM NLQTLDWDDE LLAAFRIPRA MLPRIVSSSE VYGNATLASV AGVPIAGILG 250 260 270 280 290 300 DQQAALVGQT CFHAGEAKNT YGTGCFLLMN TGTKPTPSKH GMLTTVAYRF ATQPAVYALE 310 320 330 340 350 360 GSVAITGALV QWVRDNFGLI EKSSDIEVLA RTVKDNGGVY FVPAFSGLYA PYWKDNARGV 370 380 390 400 410 420 IAGLTRYTNK GHLARAVLEA TAFQTREVVE AMEKDSAIAL ETLRTDGGMV SNDLLMQFQS 430 440 450 460 470 480 DILDRPVVRP AVQETTALGA AYAAGLAVGF FQDTDDLRSR WSVDHTWKPQ MEPERRQELY 490 SFWKKAVTRS FDWL

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ellin6076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000473 Genomic DNA. Translation: ABJ86502.1.
RefSeq	YP_826787.1. NC_008536.1.
3D structure databases
ProteinModelPortal	Q01V13.
SMR	Q01V13. Positions 2-494.
ModBase	Search...
Protein-protein interaction databases
STRING	Q01V13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4426210.
GenomeReviews	Gene locus Acid_5555 in contig CP000473_GR.
KEGG	sus:Acid_5555.
NMPDR	fig|234267.9.peg.5283.
PATRIC	32013713. VBICanSol30224_5814.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	ATYFSGT.
PhylomeDB	Q01V13.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_SOLUE

Accession

Primary (citable) accession number: Q01V13

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program