Q01UL9 (Q01UL9_SOLUE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
2-isopropylmalate synthase EMBL ABJ86651.1
EC=2.3.3.13 EMBL ABJ86651.1

Gene names

Ordered Locus Names:

Acid_5704

Organism

Solibacter usitatus (strain Ellin6076) [Complete proteome] [HAMAP] EMBL ABJ86651.1

Taxonomic identifier

234267 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fibrobacteres/Acidobacteria group › Acidobacteria › Solibacteres › Solibacterales › Solibacteraceae › Candidatus Solibacter › Solibacter usitatus

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. SAAS SAAS000891
Catalytic activity	Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. SAAS SAAS005671
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. SAAS SAAS000891
Subunit structure	Homotetramer By similarity. SAAS SAAS000891

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis SAAS SAAS005671
Molecular function	Acyltransferase EMBL ABJ86651.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2-isopropylmalate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q01UL9 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 9E1E06EBC16D37E0	FASTA	509	54,439
	10 20 30 40 50 60 MRDGEQAAGT RLGSREKLII ARQLALLNVD VIEAGYPNSS PEDFDAVQRI AAEIQGPAIC 70 80 90 100 110 120 ALSRAVAADI EACGKALAGC PRPRIHTGIG ASDIHIAGKF QDDRYGKSLA EKKRKILQMA 130 140 150 160 170 180 IDAVTLARHY VAEVEFYAED AGRADPVYLY EMIAAVIDAG ASVVNIPDTT GYTVPEQYGA 190 200 210 220 230 240 LIAGIRTHVP GIDRAVISVH CHDDLGMAVA NTLHGVLNGA RQVEGTINGL GERAGNAALE 250 260 270 280 290 300 EIVMALATRA DYFGVHTGVN TRELHRTSRL VADLLGVQVP PNKAVIGANA FSHSSGIHVD 310 320 330 340 350 360 GVLKKRETYE IMRPEDVGMT ESRVVLTART GRHGLLDRLR KLGYELSDLE LDQAYRRFLS 370 380 390 400 410 420 VADKKQEVFD EDLIAILRDE IHPLPAVYEL QYLHIYSGTS AIPTATVRLL VSGDTREGAA 430 440 450 460 470 480 IGDGPVDAVC RAISQVTATA AKLQRYEIRG VTSGTEAMGE VTIQLEEAGR KVIGRGASTD 490 500 IIEASAKAYL DGLNKLANIA AASHRSAGA

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ellin6076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000473 Genomic DNA. Translation: ABJ86651.1.
RefSeq	YP_826936.1. NC_008536.1.
3D structure databases
ProteinModelPortal	Q01UL9.
ModBase	Search...
Protein-protein interaction databases
STRING	234267.Acid_5704.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ86651; ABJ86651; Acid_5704.
GeneID	4426079.
KEGG	sus:Acid_5704.
PATRIC	32014035. VBICanSol30224_5975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0119.
HOGENOM	HOG000046859.
KO	K01649.
OMA	RTHEEVS.
Enzyme and pathway databases
BioCyc	SUSI234267:GHSK-5747-MONOMER.
UniPathway	UPA00048; UER00070.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR005671. LeuA_bact_synth. IPR000891. PYR_CT. [Graphical view]
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR00973. leuA_bact. 1 hit.
PROSITE	PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q01UL9_SOLUE

Accession

Primary (citable) accession number: Q01UL9

Entry history

Integrated into UniProtKB/TrEMBL:	November 14, 2006
Last sequence update:	November 14, 2006
Last modified:	May 29, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)