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Protein

2-isopropylmalate synthase

Gene

Acid_5704

Organism
Solibacter usitatus (strain Ellin6076)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).SAAS annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.SAAS annotation

Pathwayi

GO - Molecular functioni

  1. 2-isopropylmalate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

AcyltransferaseImported, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesisSAAS annotation

Enzyme and pathway databases

BioCyciSUSI234267:GHSK-5747-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseSAAS annotation (EC:2.3.3.13SAAS annotation)
Gene namesi
Ordered Locus Names:Acid_5704Imported
OrganismiSolibacter usitatus (strain Ellin6076)Imported
Taxonomic identifieri234267 [NCBI]
Taxonomic lineageiBacteriaAcidobacteriaSolibacteresSolibacteralesSolibacteraceaeCandidatus Solibacter
ProteomesiUP000000671 Componenti: Chromosome

Interactioni

Subunit structurei

Homotetramer.SAAS annotation

Protein-protein interaction databases

STRINGi234267.Acid_5704.

Structurei

3D structure databases

ProteinModelPortaliQ01UL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiCETICKL.
OrthoDBiEOG6CGCF3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01UL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDGEQAAGT RLGSREKLII ARQLALLNVD VIEAGYPNSS PEDFDAVQRI
60 70 80 90 100
AAEIQGPAIC ALSRAVAADI EACGKALAGC PRPRIHTGIG ASDIHIAGKF
110 120 130 140 150
QDDRYGKSLA EKKRKILQMA IDAVTLARHY VAEVEFYAED AGRADPVYLY
160 170 180 190 200
EMIAAVIDAG ASVVNIPDTT GYTVPEQYGA LIAGIRTHVP GIDRAVISVH
210 220 230 240 250
CHDDLGMAVA NTLHGVLNGA RQVEGTINGL GERAGNAALE EIVMALATRA
260 270 280 290 300
DYFGVHTGVN TRELHRTSRL VADLLGVQVP PNKAVIGANA FSHSSGIHVD
310 320 330 340 350
GVLKKRETYE IMRPEDVGMT ESRVVLTART GRHGLLDRLR KLGYELSDLE
360 370 380 390 400
LDQAYRRFLS VADKKQEVFD EDLIAILRDE IHPLPAVYEL QYLHIYSGTS
410 420 430 440 450
AIPTATVRLL VSGDTREGAA IGDGPVDAVC RAISQVTATA AKLQRYEIRG
460 470 480 490 500
VTSGTEAMGE VTIQLEEAGR KVIGRGASTD IIEASAKAYL DGLNKLANIA

AASHRSAGA
Length:509
Mass (Da):54,439
Last modified:November 14, 2006 - v1
Checksum:i9E1E06EBC16D37E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000473 Genomic DNA. Translation: ABJ86651.1.
RefSeqiYP_826936.1. NC_008536.1.

Genome annotation databases

EnsemblBacteriaiABJ86651; ABJ86651; Acid_5704.
KEGGisus:Acid_5704.
PATRICi32014035. VBICanSol30224_5975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000473 Genomic DNA. Translation: ABJ86651.1.
RefSeqiYP_826936.1. NC_008536.1.

3D structure databases

ProteinModelPortaliQ01UL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi234267.Acid_5704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ86651; ABJ86651; Acid_5704.
KEGGisus:Acid_5704.
PATRICi32014035. VBICanSol30224_5975.

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiCETICKL.
OrthoDBiEOG6CGCF3.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.
BioCyciSUSI234267:GHSK-5747-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ellin6076Imported.

Entry informationi

Entry nameiQ01UL9_SOLUE
AccessioniPrimary (citable) accession number: Q01UL9
Entry historyi
Integrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: April 1, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.