Q01NZ9 (RNPH_SOLUE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Acid_7723

Organism

Solibacter usitatus (strain Ellin6076) [Complete proteome] [HAMAP]

Taxonomic identifier

234267 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Solibacteres › Solibacterales › Solibacteraceae › Candidatus Solibacter

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP MF_00564		PRO_1000024897

Sequences

	Sequence		Length	Mass (Da)
	Q01NZ9 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 3F6F7C0288283BCC	FASTA	238	25,739
	10 20 30 40 50 60 MRTDNRTAGQ LRPVQITTGC LMTAEGSALI KVGNTHVLCA ATIEDTVPPF LRNTGKGWVT 70 80 90 100 110 120 AEYSMLPRAT AKRTPREVTK GRPSGRTHEI QRLIGRSMRS AVDMSSFGER TLIIDCDVIQ 130 140 150 160 170 180 ADGGTRTASI TGAFVAMALA FRQLIEYRAL KANPIRDYVA ATSVGLVGGI PMLDLCYEED 190 200 210 220 230 SQADVDMNVV MTGGGKFVEV QATAEHSAFD DAQMAELTEL ARTGIAQLVR MQKEIIEG

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ellin6076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000473 Genomic DNA. Translation: ABJ88621.1.
RefSeq	YP_828906.1. NC_008536.1.
3D structure databases
ProteinModelPortal	Q01NZ9.
SMR	Q01NZ9. Positions 2-236.
ModBase	Search...
Protein-protein interaction databases
STRING	Q01NZ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4431339.
GenomeReviews	Gene locus Acid_7723 in contig CP000473_GR.
KEGG	sus:Acid_7723.
NMPDR	fig|234267.9.peg.7326.
PATRIC	32018226. VBICanSol30224_8060.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q01NZ9.
ProtClustDB	PRK00173.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_SOLUE

Accession

Primary (citable) accession number: Q01NZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program