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Q01N68 (Q01N68_SOLUE) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Ordered Locus Names:Acid_2399
OrganismSolibacter usitatus (strain Ellin6076) [Complete proteome] [HAMAP]
Taxonomic identifier234267 [NCBI]
Taxonomic lineageBacteria › Acidobacteria › Solibacteres › Solibacterales › Solibacteraceae › Candidatus Solibacter

Protein attributes

Sequence length934 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q01N68 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: B4D3F2802CB635E9

FASTA934106,398
        10         20         30         40         50         60 
MPKALVIVES PAKAKTINKY LGKQYVVKAS LGHIKDLPKR DLAVDVEHGF EPRYEIIEGK 

        70         80         90        100        110        120 
KKLMGELRSA AKGVDQIYLA ADPDREGEAI CFHLQEELRS KKDGPAFFRV MFNEITKKAV 

       130        140        150        160        170        180 
EKAFEKPLQV NTNLVDAQQA RRVLDRLVGY KISPLLWDKV RRGLSAGRVQ TVALRVIVER 

       190        200        210        220        230        240 
EREIRAFQKK EYWTVDVDLL ARTAPVLSTR LIKANDETPE LPNQQTTDSL VAQLSGVDYI 

       250        260        270        280        290        300 
VKSVATREKR RNPVAPFITS TLQQDSSRKL RFSVKRTMML AQRLYEGIEI GRDGAVGLIT 

       310        320        330        340        350        360 
YMRTDSTRTS DDAISDVRHY IGERYGAPFL PPAPNVYKSK KEAQDAHEAI RPTSMAYSPE 

       370        380        390        400        410        420 
VVEQYLPEDE MKLYRLIWNR FVASQMMPAL FDQTTIDVSA KAANGVEYTF RATGSVPKFD 

       430        440        450        460        470        480 
GFLKVYQEGK DQTDEDDEEL KHRLPLVTEG EVLRLEAIRP EQHFTEPPPR YNEATLVKRL 

       490        500        510        520        530        540 
EADGVGRPST YATILSTIQE REYVNKEAGR FTPTELGMVV TDLLLESFDD IFDVKYTARM 

       550        560        570        580        590        600 
EEELDEIEEG KLDWRAAMSE FYGRFDKDLK HAEEHMTDIK RMEKPTDLIC EKCGRPLVIK 

       610        620        630        640        650        660 
WGKHGSFIAC TGYPDFSSKS IESDLEKVQK DIEVRRQRAL KKIELSLEKL RAKYPDRTIT 

       670        680        690        700        710        720 
WGQDGEFLVI PSAPKIPKPM DAALKKLEKE IAVKRSEDRG KIEARLEKLQ ARYPQMKGFY 

       730        740        750        760        770        780 
DLDLRDTAEG VALTVRRKEE FCTYTRELTV DLPDLEGADL SEQGEEESCE NCGRPMVLKK 

       790        800        810        820        830        840 
GRFGTFFACS EYPDCKTTRQ IGGTQKKDVP LDEKCPQCDN NLVMKYGRFG EFTACSNYPT 

       850        860        870        880        890        900 
CKYVKQKTIG VKCPECSEGD IIERRSKKGK TFYGCNRYPD CTFVAWGKPV AEKCPECGSP 

       910        920        930 
YMIEKFLKSG AFWQCPNAEC KHQVEAPAPV EVTS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000473 Genomic DNA. Translation: ABJ83388.1.
RefSeqYP_823673.1. NC_008536.1.

3D structure databases

ProteinModelPortalQ01N68.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ01N68.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4429417.
GenomeReviewsGene locus Acid_2399 in contig CP000473_GR.
KEGGsus:Acid_2399.
NMPDRfig|234267.9.peg.2296.
PATRIC32007075. VBICanSol30224_2512.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0550.
HOGENOMHBG512935.
OMATLIWKRF.
PhylomeDBQ01N68.
ProtClustDBPRK07561.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 4 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ01N68_SOLUE
AccessionPrimary (citable) accession number: Q01N68
Entry history
Integrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info