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Protein

Probable L-ascorbate peroxidase 3

Gene

APX3

Organism
Oryza sativa subsp. indica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei37Transition state stabilizerPROSITE-ProRule annotation1
Active sitei41Proton acceptorPROSITE-ProRule annotation1
Metal bindingi161Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi162Potassium or calciumBy similarity1
Metal bindingi178Potassium or calciumBy similarity1
Metal bindingi185Potassium or calciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L-ascorbate peroxidase 3 (EC:1.11.1.11)
Alternative name(s):
OsAPx03
Gene namesi
Name:APX3
ORF Names:H0515C11.2, OsI_014375
OrganismiOryza sativa subsp. indica (Rice)
Taxonomic identifieri39946 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000007015 Componenti: Chromosome 4

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003002581 – 291Probable L-ascorbate peroxidase 3Add BLAST291

Interactioni

Protein-protein interaction databases

STRINGi39946.BGIOSGA015546-PA.

Structurei

3D structure databases

ProteinModelPortaliQ01MI9.
SMRiQ01MI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF5T. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
OMAiRDSQDEF.
OrthoDBiEOG09360K6Y.

Family and domain databases

InterProiView protein in InterPro
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR002207. Peroxidase_I.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
PfamiView protein in Pfam
PF00141. peroxidase. 1 hit.
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiView protein in PROSITE
PS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01MI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAPVVDAE YMAEVERARR DLRALIASKS CAPIMLRLAW HDAGTYDKAT
60 70 80 90 100
KTGGPNGSIR FPQEYSHAAN AGIKIAIDLL EPMKQRHPKI TYADLYQLAG
110 120 130 140 150
VVAVEVTGGP TIDYVPGRRD SSDSPEEGRL PDAKKGAAHL REVFYRMGLS
160 170 180 190 200
DKDIVALSGG HTLGKARPER SGFDGAWTKD PLKFDNSYFI ELLKENSEGL
210 220 230 240 250
LKLPTDKALV EDPTFRRYVE LYAKDEDAFF RDYAESHKKL SELGFTPPRS
260 270 280 290
AFIYKSCQKP KSLLMQTAAG VAVAAAVVAW AYLCESNKRL G
Length:291
Mass (Da):32,076
Last modified:November 14, 2006 - v1
Checksum:i9D2E57C3DBC277F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY382617 mRNA. Translation: AAQ88105.1.
CR855043 Genomic DNA. Translation: CAH66026.1.
CM000129 Genomic DNA. Translation: EAY93142.1.

Genome annotation databases

EnsemblPlantsiBGIOSGA015546-TA; BGIOSGA015546-PA; BGIOSGA015546.
GrameneiBGIOSGA015546-TA; BGIOSGA015546-PA; BGIOSGA015546.

Similar proteinsi

Entry informationi

Entry nameiAPX3_ORYSI
AccessioniPrimary (citable) accession number: Q01MI9
Secondary accession number(s): A2XQN2, Q6TY83, Q7XWZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 14, 2006
Last modified: October 25, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

According to PubMed:15599508, it may be peroxisomal. There is however no experimental evidence to prove this.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families