Q01IS8 (INV3_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 3
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 3
Invertase 3
OsCIN3
Sucrose hydrolase 3

Gene names

Name:	CIN3
Synonyms:	INV4
ORF Names:	OSIGBa0134P10.8, OsI_015274

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Sequence caution	The sequence CAH67362.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 586	560	Beta-fructofuranosidase, insoluble isoenzyme 3		PRO_0000303012
Sites
	Active site	61	1	By similarity
Amino acid modifications
	Glycosylation	154	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	341	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	479	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	89	1	E → K in AAQ24870. Ref.2
	Sequence conflict	152	1	P → L in AAQ24870. Ref.2
	Sequence conflict	164	1	Y → H in AAQ24870. Ref.2
	Sequence conflict	172	1	I → V in AAQ24870. Ref.2
	Sequence conflict	375	1	Q → R in AAQ24870. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q01IS8 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: C7D262407F51245F	FASTA	586	66,208
	10 20 30 40 50 60 MATARARAAL VFVALLQMAA VVVVRASHVV YPELQSLEAK HVDGKLRTGY HFQPPKHWIN 70 80 90 100 110 120 DPNGPMYYKG LYHLFYQYNP KGAVWGNIEW AHSVSTDLID WTALEPGIYP SKTFDEKGCW 130 140 150 160 170 180 SGSATVLPSG VPVIMYTGID PDERQVQNVA YPVNLSDPYL REWYKPDYNP IINPDGGINA 190 200 210 220 230 240 SAFRDPTTAW YGPDGHWRLL VGSKVNMKGL AVLYRSRDFK KWVKAHHPLH SAHTGMWECP 250 260 270 280 290 300 DFFPVAVAGG SRHYRRGVDT AELHDAAVAE EVKYVLKVSL DLTRYEYYTV GWYDHATDRY 310 320 330 340 350 360 VPDAAFPDND YGLRYDYGDF YASKSFYDPA KRRRIVWGWA NESDTVPDDR RKGWAGIQAI 370 380 390 400 410 420 PRKLWLSADG KQLVQWPVEE LKALRAKHVN VTDKVIKKGN YFEVTGFKSV QSDVDMAFAI 430 440 450 460 470 480 KDLSKAEEFD PAWRTDAEAL CKKLGSDVDG GVGPFGLWAL ASGDLKERTA VFFRVFKAND 490 500 510 520 530 540 SSHVVLMCND PTRSSYESKI YRPTFAGFVD VDIAKNKQIA LRTLIDHSVV ESFGARGKTC 550 560 570 580 ILTRVYPRKA VGDDAHLFVF NNGESDVKVT NLDAWEMKTP KMNAEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of four invertase genes with expression in the rice anther." Oliver S.N., Fernandes S.Q., Dennis E.S., Dolferus R. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. IR36.
[2]	"Characterization of three rice cell wall invertase genes." Wang Y.-Q., Zhu Z. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Minghui 86.
[3]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Guang-Lu-Ai No.4.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY220486 Genomic DNA. Translation: AAO63553.1. AY342320 mRNA. Translation: AAQ24870.1. CR855194 Genomic DNA. Translation: CAH67362.1. Sequence problems. CM000129 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	Q01IS8.
SMR	Q01IS8. Positions 44-583.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Proteomic databases
PRIDE	Q01IS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os04g33720.1; LOC_Os04g33720.1; LOC_Os04g33720.
Organism-specific databases
Gramene	Q56UD3.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV3_ORYSI

Accession

Primary (citable) accession number: Q01IS8
Secondary accession number(s): A2XT81 , Q56UD3, Q6VEF3, Q7XVJ3, Q84TL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program