Q01C49 (Q01C49_OSTTA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Cobalamin-dependent methionine synthase (ISS) EMBL CAL53105.1

Gene names

Ordered Locus Names:

Ot03g04540 EMBL CAL53105.1

Organism

Ostreococcus tauri [Complete proteome] EMBL CAL53105.1

Taxonomic identifier

70448 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Chlorophyta › Mamiellophyceae › Mamiellales › Ostreococcus

Protein attributes

Sequence length	1324 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit By similarity. PIRSR PIRSR000381-1 Cobalamin By similarity. PIRSR PIRSR000381-2
Caution	The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data. EMBL CAL53105.1

Ontologies

Keywords
Ligand	Cobalamin PIRSR PIRSR000381-2 Cobalt Metal-binding PIRSR PIRSR000381-1 S-adenosyl-L-methionine PIRSR PIRSR000381-2 Zinc PIRSR PIRSR000381-1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pteridine-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro methionine synthase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	924 – 925	2	Cobalamin-binding By similarity PIRSR PIRSR000381-2
	Region	1287 – 1288	2	S-adenosyl-L-methionine binding By similarity PIRSR PIRSR000381-2
Sites
	Metal binding	322	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	385	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	386	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	849	1	Cobalt (cobalamin axial ligand) By similarity PIRSR PIRSR000381-1
	Binding site	1038	1	S-adenosyl-L-methionine By similarity PIRSR PIRSR000381-2
	Binding site	1232	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity PIRSR PIRSR000381-2
	Binding site	1236	1	Cobalamin; via carbonyl oxygen By similarity PIRSR PIRSR000381-2
Experimental info
	Non-terminal residue	1324	1	EMBL CAL53105.1

Sequences

	Sequence		Length	Mass (Da)
	Q01C49 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 5A01F66A6FAB5326	FASTA	1,324	145,764
	10 20 30 40 50 60 XXADATERML EFAATLDPKS KPTQLRRLGA EPPAPKFEAQ PKQNYSSENK IAPKKASELL 70 80 90 100 110 120 PVPTYKEFVD PMKKSPAFDV IEGIMAKRVM VIDGAMGTAV QRYKLTEDDF RGERYKEHTH 130 140 150 160 170 180 DLKGNNDILV LTRPDVIGEI HEAYLAAGAD IIETNTFSST MIAQADYELD KKNEVRDMNI 190 200 210 220 230 240 AAARLAKAAC EKFTKQNPDK PRFAAGAIGP TNRTLSVSPS VENPAFRACT FDEIVDGYYD 250 260 270 280 290 300 QIEALVEGGV DMLLVETIFD TLNAKAAIFA IEKFFDAYGT RLPLFVSGTI VDNSGRTLSG 310 320 330 340 350 360 QTNEAFWNSV MHAKPMAIGL NCALGAKDMV PYIENLSKCA DCWVFAYPNA GLPNAMGGYD 370 380 390 400 410 420 QKGAEMAEDC RVFLEKGLLN GIGGCCGTTD EHIGALADMV APYTPRKKHG IPDIMRLSGL 430 440 450 460 470 480 EPFNYSPDEK DYRKTFINLG ERCNVAGSTI FKKAIVDGDF EKALAIALKQ VENGAHVIDI 490 500 510 520 530 540 NMDDGLIDGE SAMTRFVNLL VSEPDASKVP FMIDSSKFHV VEAGLKCSQG KCIMNSISLK 550 560 570 580 590 600 GGEEEFLKHA RIVKRHGAAV VVMAFDEAGQ AATEAEKIRI CCRAYKLLVE EVGFNPQDII 610 620 630 640 650 660 FDPNILTIGT GMEEHNNYAV DFINATREIK RLCPGCKISG GVSNLAFSFR GNEPVRRAFH 670 680 690 700 710 720 SAFLYHACKA GMDMGIVNAA QELLEYVEDV LLNRCTNATE RMLEFAATLD PKSKPTELRK 730 740 750 760 770 780 KGVAATSKKT AGSGKEWRNQ PAEKRLEYAL IKGIDEFCIA DTEECRIGGK YSSCLEVIEG 790 800 810 820 830 840 PLMDGMNIVG DLFGAGKMFL PQVIKSARVM KKAVGHLIPF MDAEKAATGS TEASTAGTFL 850 860 870 880 890 900 IATVKGDVHD IGKNIVAVVL GCNNFKVVDI GVMCPCEKIL DAVVEHKADI LGLSGLITPS 910 920 930 940 950 960 LDEMVTVAKE MEKRGMKLPL LIGGATTSKM HTAVKLTPNY SGGVIHVLDA SRAVPVAQTL 970 980 990 1000 1010 1020 LDDRKNVDFL DDINETYAEM REEFYAGLED RKYLTLEKAR ATVQPVDFTV APNIPVTPKF 1030 1040 1050 1060 1070 1080 CGAKSVEVSI DDVLPYIDWN PFFQVWQLRG RYPNRGYPKI FNDETVGSEA KKLFNEANAM 1090 1100 1110 1120 1130 1140 IQKIKENKRL TLKGIYGFYP ANSVGDDIVV YTDETRSTPQ HTFFTLRQQA EKDTDEPYMA 1150 1160 1170 1180 1190 1200 LSDFIAPKSS GVKDYLGMFV CSAGFGLDEL TAEYKAANDD YSYIMAEALA DRLAEAFAEL 1210 1220 1230 1240 1250 1260 LHEQVRKDHW GYAKDEAFSC DDLLKVKYQG IRPAPGYPSQ PDHTEKATMW DLMKVKEEIG 1270 1280 1290 1300 1310 1320 VELTETFAML PSASVSGLYF AGACSQYFNV GKITADQVTE YADRKKMDLS EAQRWLGPNL SYEP

References

[1]

"Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features." Derelle E., Ferraz C., Rombauts S., Rouze P., Worden A.Z., Robbens S., Partensky F., Degroeve S., Echeynie S., Cooke R., Saeys Y., Wuyts J., Jabbari K., Bowler C., Panaud O., Piegu B., Ball S.G., Ral J.-P. , Bouget F.-Y., Piganeau G., De Baets B., Picard A., Delseny M., Demaille J., Van de Peer Y., Moreau H. Proc. Natl. Acad. Sci. U.S.A. 103:11647-11652(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OTTH0595.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CAID01000003 Genomic DNA. Translation: CAL53105.1.
RefSeq	XP_003078364.1. XM_003078316.1.
3D structure databases
ProteinModelPortal	Q01C49.
ModBase	Search...
Protein-protein interaction databases
STRING	70448.Q01C49. Q01C49.
Proteomic databases
PRIDE	Q01C49.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9833122.
KEGG	ota:Ot03g04540.
Phylogenomic databases
eggNOG	COG1410.
HOGENOM	HOG000251409.
KO	K00548.
OMA	VRKEFWG.
Family and domain databases
Gene3D	1.10.1240.10. 1 hit. 3.10.196.10. 1 hit. 3.20.20.20. 1 hit. 3.20.20.330. 1 hit. 3.40.50.280. 1 hit.
InterPro	IPR003759. Cbl-bd_cap. IPR006158. Cobalamin-bd. IPR011005. Dihydropteroate_synth-like. IPR011822. MetH. IPR000489. Pterin-binding. IPR003726. S_MeTrfase. IPR004223. VitB12-dep_Met_synth_activ_dom. [Graphical view]
PANTHER	PTHR21091:SF9. PTHR21091:SF9. 1 hit.
Pfam	PF02310. B12-binding. 1 hit. PF02607. B12-binding_2. 1 hit. PF02965. Met_synt_B12. 1 hit. PF00809. Pterin_bind. 1 hit. PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF000381. MetH. 1 hit.
SMART	SM01018. B12-binding_2. 1 hit. [Graphical view]
SUPFAM	SSF52242. Cbl-bd. 1 hit. SSF51717. DHP_synth_like. 1 hit. SSF56507. Met_synth_B12. 1 hit. SSF47644. Met_synth_Cbl-bd. 1 hit. SSF82282. S_methyl_trans. 1 hit.
TIGRFAMs	TIGR02082. metH. 1 hit.
PROSITE	PS50974. ADOMET_ACTIVATION. 1 hit. PS51332. B12_BINDING. 1 hit. PS51337. B12_BINDING_NTER. 1 hit. PS50970. HCY. 1 hit. PS50972. PTERIN_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q01C49_OSTTA

Accession

Primary (citable) accession number: Q01C49

Entry history

Integrated into UniProtKB/TrEMBL:	November 14, 2006
Last sequence update:	November 14, 2006
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)