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Q01995 (TAGL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transgelin
Alternative name(s):
22 kDa actin-binding protein
Protein WS3-10
Smooth muscle protein 22-alpha
Short name=SM22-alpha
Gene names
Name:TAGLN
Synonyms:SM22, WS3-10
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length201 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Actin cross-linking/gelling protein By similarity. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

Subcellular location

Cytoplasm Probable.

Induction

Overexpressed in senescent human fibroblasts.

Sequence similarities

Belongs to the calponin family.

Contains 1 calponin-like repeat.

Contains 1 CH (calponin-homology) domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandActin-binding
   Molecular functionMuscle protein
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processepithelial cell differentiation

Inferred from direct assay PubMed 21492153. Source: UniProt

muscle organ development

Traceable author statement Ref.3. Source: ProtInc

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

IGF1RP080692EBI-1054248,EBI-475981

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 201200Transgelin
PRO_0000204781

Regions

Domain24 – 137114CH
Repeat175 – 20026Calponin-like

Amino acid modifications

Modified residue21N-acetylalanine Ref.8
Modified residue1661Phosphoserine Ref.7
Modified residue1721N6-acetyllysine By similarity

Natural variations

Natural variant1821N → S.
Corresponds to variant rs12284316 [ dbSNP | Ensembl ].
VAR_048670

Experimental info

Sequence conflict491R → P in AAA58351. Ref.1
Sequence conflict721D → E in AAA58375. Ref.2
Sequence conflict1041Y → C in AAA58375. Ref.2
Sequence conflict1041Y → S in AAA58351. Ref.1
Sequence conflict1791M → V in AAA58375. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q01995 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 7AE9E7A9A45602C3

FASTA20122,611
        10         20         30         40         50         60 
MANKGPSYGM SREVQSKIEK KYDEELEERL VEWIIVQCGP DVGRPDRGRL GFQVWLKNGV 

        70         80         90        100        110        120 
ILSKLVNSLY PDGSKPVKVP ENPPSMVFKQ MEQVAQFLKA AEDYGVIKTD MFQTVDLFEG 

       130        140        150        160        170        180 
KDMAAVQRTL MALGSLAVTK NDGHYRGDPN WFMKKAQEHK REFTESQLQE GKHVIGLQMG 

       190        200 
SNRGASQAGM TGYGRPRQII S 

« Hide

References

« Hide 'large scale' references
[1]"A novel gene encoding a smooth muscle protein is overexpressed in senescent human fibroblasts."
Thweatt R., Lumpkin C.K. Jr., Goldstein S.
Biochem. Biophys. Res. Commun. 187:1-7(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fibroblast.
[2]"Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle."
Nishida W., Kitami Y., Abe M., Kiwada K.
Biochem. Int. 23:663-668(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Expression and cytogenetic localization of the human SM22 gene (TAGLN)."
Camoretti-Mercado B., Forsythe S.M., Lebeau M.M., Espinosa R.D. III, Vieira J.E., Halayko A.J., Willadsen S., Kurtz B., Ober C., Evans G.A., Thweatt R., Shapiro S., Niu Q., Qin Y., Padrid P.A., Solway J.
Genomics 49:452-457(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Structure and expression of the human SM22alpha gene, assignment of the gene to chromosome 11, and repression of the promoter activity by cytosine DNA methylation."
Yamamura H., Masuda H., Ikeda W., Tokuyama T., Takagi M., Shibata N., Tatsuta M., Takahashi K.
J. Biochem. 122:157-167(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Aorta and Peripheral blood.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Uterus.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95787 mRNA. Translation: AAA58351.1.
M83106 mRNA. Translation: AAA58375.1.
AF013711 Genomic DNA. Translation: AAC21582.1.
D17409 mRNA. Translation: BAA21811.1.
D84342 Genomic DNA. Translation: BAA21839.1.
BC004927 mRNA. Translation: AAH04927.1.
BC065829 mRNA. Translation: AAH65829.1.
PIRJS0774. JC5577.
RefSeqNP_001001522.1. NM_001001522.1.
NP_003177.2. NM_003186.3.
UniGeneHs.410977.

3D structure databases

ProteinModelPortalQ01995.
SMRQ01995. Positions 24-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112739. 18 interactions.
IntActQ01995. 2 interactions.
MINTMINT-2805284.
STRING9606.ENSP00000278968.

PTM databases

PhosphoSiteQ01995.

Polymorphism databases

DMDM3123283.

2D gel databases

REPRODUCTION-2DPAGEIPI00216138.
UCD-2DPAGEQ01995.

Proteomic databases

PaxDbQ01995.
PeptideAtlasQ01995.
PRIDEQ01995.

Protocols and materials databases

DNASU6876.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000278968; ENSP00000278968; ENSG00000149591.
ENST00000392951; ENSP00000376678; ENSG00000149591.
ENST00000525531; ENSP00000432054; ENSG00000149591.
ENST00000530649; ENSP00000431941; ENSG00000149591.
ENST00000532870; ENSP00000432282; ENSG00000149591.
GeneID6876.
KEGGhsa:6876.
UCSCuc001pqm.3. human.

Organism-specific databases

CTD6876.
GeneCardsGC11P117070.
HGNCHGNC:11553. TAGLN.
HPACAB001447.
HPA019467.
MIM600818. gene.
neXtProtNX_Q01995.
PharmGKBPA36324.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5199.
HOGENOMHOG000232113.
HOVERGENHBG005186.
InParanoidQ01995.
OMAVEWIVVQ.
OrthoDBEOG718KD9.
PhylomeDBQ01995.
TreeFamTF313921.

Gene expression databases

ArrayExpressQ01995.
BgeeQ01995.
CleanExHS_TAGLN.
GenevestigatorQ01995.

Family and domain databases

Gene3D1.10.418.10. 1 hit.
InterProIPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamPF00402. Calponin. 1 hit.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSPR00888. SM22CALPONIN.
SMARTSM00033. CH. 1 hit.
[Graphical view]
SUPFAMSSF47576. SSF47576. 1 hit.
PROSITEPS01052. CALPONIN_1. 1 hit.
PS51122. CALPONIN_2. 1 hit.
PS50021. CH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTAGLN. human.
GeneWikiTAGLN.
GenomeRNAi6876.
NextBio26849.
PROQ01995.
SOURCESearch...

Entry information

Entry nameTAGL_HUMAN
AccessionPrimary (citable) accession number: Q01995
Secondary accession number(s): O15542
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 140 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM