Q01995 (TAGL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transgelin

Alternative name(s):
22 kDa actin-binding protein
Protein WS3-10
Smooth muscle protein 22-alpha
Short name=SM22-alpha

Gene names

Name:	TAGLN
Synonyms:	SM22, WS3-10

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Actin cross-linking/gelling protein By similarity. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Subcellular location	Cytoplasm Probable.
Induction	Overexpressed in senescent human fibroblasts.
Sequence similarities	Belongs to the calponin family. Contains 1 calponin-like repeat. Contains 1 CH (calponin-homology) domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	Actin-binding
Molecular function	Muscle protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	muscle organ development Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 201	200	Transgelin		PRO_0000204781
Regions
	Domain	24 – 137	114	CH
	Repeat	175 – 200	26	Calponin-like
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Potential
	Modified residue	17	1	N6-acetyllysine Ref.7
	Modified residue	193	1	Phosphotyrosine Ref.6
Natural variations
	Natural variant	182	1	N → S. Corresponds to variant rs12284316 [ dbSNP | Ensembl ].		VAR_048670
Experimental info
	Sequence conflict	49	1	R → P in AAA58351. Ref.1
	Sequence conflict	72	1	D → E in AAA58375. Ref.2
	Sequence conflict	104	1	Y → C in AAA58375. Ref.2
	Sequence conflict	104	1	Y → S in AAA58351. Ref.1
	Sequence conflict	179	1	M → V in AAA58375. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q01995 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 7AE9E7A9A45602C3	FASTA	201	22,611
	10 20 30 40 50 60 MANKGPSYGM SREVQSKIEK KYDEELEERL VEWIIVQCGP DVGRPDRGRL GFQVWLKNGV 70 80 90 100 110 120 ILSKLVNSLY PDGSKPVKVP ENPPSMVFKQ MEQVAQFLKA AEDYGVIKTD MFQTVDLFEG 130 140 150 160 170 180 KDMAAVQRTL MALGSLAVTK NDGHYRGDPN WFMKKAQEHK REFTESQLQE GKHVIGLQMG 190 200 SNRGASQAGM TGYGRPRQII S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gene encoding a smooth muscle protein is overexpressed in senescent human fibroblasts." Thweatt R., Lumpkin C.K. Jr., Goldstein S. Biochem. Biophys. Res. Commun. 187:1-7(1992) [PubMed: 1520290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fibroblast.
[2]	"Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle." Nishida W., Kitami Y., Abe M., Kiwada K. Biochem. Int. 23:663-668(1991) [PubMed: 1872880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Expression and cytogenetic localization of the human SM22 gene (TAGLN)." Camoretti-Mercado B., Forsythe S.M., Lebeau M.M., Espinosa R.D. III, Vieira J.E., Halayko A.J., Willadsen S., Kurtz B., Ober C., Evans G.A., Thweatt R., Shapiro S., Niu Q., Qin Y., Padrid P.A., Solway J. Genomics 49:452-457(1998) [PubMed: 9615232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Structure and expression of the human SM22alpha gene, assignment of the gene to chromosome 11, and repression of the promoter activity by cytosine DNA methylation." Yamamura H., Masuda H., Ikeda W., Tokuyama T., Takagi M., Shibata N., Tatsuta M., Takahashi K. J. Biochem. 122:157-167(1997) [PubMed: 9276683] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Aorta and Peripheral blood.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-193, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-17, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95787 mRNA. Translation: AAA58351.1. M83106 mRNA. Translation: AAA58375.1. AF013711 Genomic DNA. Translation: AAC21582.1. D17409 mRNA. Translation: BAA21811.1. D84342 Genomic DNA. Translation: BAA21839.1. BC004927 mRNA. Translation: AAH04927.1. BC065829 mRNA. Translation: AAH65829.1.
IPI	IPI00216138.
PIR	JS0774. JC5577.
RefSeq	NP_001001522.1. NM_001001522.1. NP_003177.2. NM_003186.3.
UniGene	Hs.410977. Hs.666842.
3D structure databases
ProteinModelPortal	Q01995.
SMR	Q01995. Positions 24-154.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q01995. 1 interaction.
STRING	Q01995.
PTM databases
PhosphoSite	Q01995.
Polymorphism databases
DMDM	3123283.
2D gel databases
PMMA-2DPAGE	Q01995.
REPRODUCTION-2DPAGE	IPI00216138.
UCD-2DPAGE	Q01995.
Proteomic databases
PeptideAtlas	Q01995.
PRIDE	Q01995.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000278968; ENSP00000278968; ENSG00000149591. ENST00000392951; ENSP00000376678; ENSG00000149591.
GeneID	6876.
KEGG	hsa:6876.
UCSC	uc001pqm.1. human.
Organism-specific databases
CTD	6876.
GeneCards	GC11P117070.
H-InvDB	HIX0010162.
HGNC	HGNC:11553. TAGLN.
HPA	CAB001447. HPA019467.
MIM	600818. gene.
neXtProt	NX_Q01995.
PharmGKB	PA36324.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09514.
HOGENOM	HBG396331.
HOVERGEN	HBG005186.
InParanoid	Q01995.
OMA	RLVEWIV.
OrthoDB	EOG4DV5N5.
PhylomeDB	Q01995.
Gene expression databases
ArrayExpress	Q01995.
Bgee	Q01995.
CleanEx	HS_TAGLN.
Genevestigator	Q01995.
GermOnline	ENSG00000149591. Homo sapiens.
Family and domain databases
InterPro	IPR000557. Calponin_repeat. IPR001715. CH-domain. IPR003096. SM22_calponin. IPR001061. Transgelin. [Graphical view]
Gene3D	G3DSA:1.10.418.10. Calponin-homology. 1 hit.
PANTHER	PTHR18959:SF5. Transgelin. 1 hit.
Pfam	PF00402. Calponin. 1 hit. PF00307. CH. 1 hit. [Graphical view]
PRINTS	PR00888. SM22CALPONIN. PR00890. TRANSGELIN.
SMART	SM00033. CH. 1 hit. [Graphical view]
SUPFAM	SSF47576. Calponin-homology. 1 hit.
PROSITE	PS01052. CALPONIN_1. 1 hit. PS51122. CALPONIN_2. 1 hit. PS50021. CH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	26849.
SOURCE	Search...

Entry information

Entry name

TAGL_HUMAN

Accession

Primary (citable) accession number: Q01995
Secondary accession number(s): O15542

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 121 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.