Reviewed, UniProtKB/Swiss-Prot Q01994 (RIB1_PHOLE)

Names and origin

Protein names

Recommended name:
    6,7-dimethyl-8-ribityllumazine synthase
      Short name=DMRL synthase
      Short name=Lumazine synthase
    EC=2.5.1.9
Alternative name(s):
    Riboflavin synthase beta chain

Gene names

Name:

ribH

Organism

Photobacterium leiognathi

Taxonomic identifier

553611 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	144 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›144	›144	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_0000134779
Experimental info
	Non-terminal residue	144	1

Sequences

	Sequence		Length	Mass (Da)
	Q01994-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 4A4E4820CD960359	FASTA	144	15,181
	10 20 30 40 50 60 MKLLKGVDCT SCCIAIVIAR FNSFINENLL SGAINALQRK GQVKAENITV IRCPGAYELP 70 80 90 100 110 120 LAAQQIAKQG NYDAIIAIGA VIRGGTPHFD FVAGECNKGL AQVALEYQTP VAFGVLTVDS 130 140 IEQAIERAGT KMGNKGEEAA LSAL

References

[1]

"The lux genes in Photobacterium leiognathi are closely linked with genes corresponding in sequence to riboflavin synthesis genes." Lee C.Y., Meighen E.A. Biochem. Biophys. Res. Commun. 186:690-697(1992) [PubMed: 1339274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25521 / L1 / CIP 665.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M90094 Genomic DNA. Translation: AAA73230.1.
PIR	PC1110.
3D structure databases
HSSP	HSSP built from PDB template 1RVV based on UniProtKB P11998.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.9. 349.
Family and domain databases
HAMAP	MF_00178. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
ProDom	PD003664. DMRL_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00114. lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIB1_PHOLE

Accession

Primary (citable) accession number: Q01994

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	September 22, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)