Q01993 (RISA_PHOLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase alpha chain
EC=2.5.1.9

Gene names

Name:	ribE
Synonyms:	ribB

Organism

Photobacterium leiognathi

Taxonomic identifier

553611 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Riboflavin synthase alpha chain		PRO_0000068170
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 194	97	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	Q01993 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: DD0F8996FCC6173E	FASTA	218	24,095
	10 20 30 40 50 60 MFTGIIESIG NIGAIIRHNE DLSIVVNTNN LDISDVNIGD SIATNGVCLT VSKLLPSGYT 70 80 90 100 110 120 ADLSLETYKR TAFHSYRIGQ EVNLEKAMLP TTRLGGHLVS GHVDGVGEVI EFKRNGRAIN 130 140 150 160 170 180 IWVAVPVQLK KYLSEKGSVT IDGISLTINA VYQNVIKLTI VPHTLAETNL VNINIDKKVN 190 200 210 VEIDMMARYL EKLIKVDRYE SEKTSNVSMD LERYGFIS

References

[1]	"The lux genes in Photobacterium leiognathi are closely linked with genes corresponding in sequence to riboflavin synthesis genes." Lee C.Y., Meighen E.A. Biochem. Biophys. Res. Commun. 186:690-697(1992) [PubMed: 1339274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25521 / L1 / CIP 665.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90094 Genomic DNA. Translation: AAA73228.1.
PIR	JC1187.
3D structure databases
ProteinModelPortal	Q01993.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR023366. ATPase_F1/A1-cplx_a_su_N. IPR001783. Lumazine-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:2.40.30.20. G3DSA:2.40.30.20. 2 hits.
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 2 hits.
TIGRFAMs	TIGR00187. RibE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_PHOLE

Accession

Primary (citable) accession number: Q01993

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	October 19, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program