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Q01992

- MIPEP_RAT

UniProt

Q01992 - MIPEP_RAT

Protein

Mitochondrial intermediate peptidase

Gene

Mipep

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Cleaves proteins, imported into the mitochondrion, to their mature size.

    Catalytic activityi

    Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

    Cofactori

    Binds 1 zinc ion.By similarity

    Enzyme regulationi

    Activity is divalent cation-dependent. It is stimulated by manganese, magnesium or calcium ions and reversibly inhibited by zinc, cobalt and iron.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi492 – 4921Zinc; catalyticPROSITE-ProRule annotation
    Active sitei493 – 4931PROSITE-ProRule annotation
    Metal bindingi496 – 4961Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi499 – 4991Zinc; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Cobalt, Iron, Magnesium, Manganese, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM03.A05.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial intermediate peptidase (EC:3.4.24.59)
    Short name:
    MIP
    Gene namesi
    Name:Mipep
    Synonyms:Mip
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621680. Mipep.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333Mitochondrion1 PublicationAdd
    BLAST
    Chaini34 – 710677Mitochondrial intermediate peptidasePRO_0000028580Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei124 – 1241N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ01992.
    PRIDEiQ01992.

    PTM databases

    PhosphoSiteiQ01992.

    Expressioni

    Gene expression databases

    GenevestigatoriQ01992.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000062641.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01992.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M3 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0339.
    HOGENOMiHOG000230535.
    HOVERGENiHBG008215.
    InParanoidiQ01992.
    KOiK01410.

    Family and domain databases

    Gene3Di1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProiIPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view]
    PfamiPF01432. Peptidase_M3. 1 hit.
    [Graphical view]
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01992-1 [UniParc]FASTAAdd to Basket

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    MLLAAGTRYA YRLCGRRAAA ALQGRAGRSC ARSVSTSWSP VGAAFNVKPQ    50
    GHLWDLLGER RGLFGVPELS TPEGFQVAQE EALRKTEWLV ERACSTPPGP 100
    QTVLIFDELS DCLCRVADLA DFVKIGHPEQ AFREAAQEAC RSIGTMVEKL 150
    NTNVELYQSL QKLLDDKKLM DSLDAETRRV AELFMFDFEI SGIHLDEEKR 200
    RRAVDLNVKI LDLSSAFLMG TNFPIKIQKH LLPEHIQHHF ARDGRHLVID 250
    GLHAEASDDL VREAAYKIFL YPNADQLKCL EELLSSRDLL ANLVGYLPFP 300
    TGPPGTIAQT PETVMQFLEK LSEKLCERTR KDFEMMQGMK TKLNPQNSEL 350
    MPWDPPYYSG VIRAERYNIE PSLYCPFLSL GACMEGLNVL FNRLLGVTLY 400
    AEQPFKGEVW CIDVRKLAVV HESEGLLGYI YCDFFQRANK PQQDCHFTIR 450
    GGRLKEDGSY QLPVVVLMLN LPHASRDFPT LLTPGMMENL FHEMGHAMHS 500
    MLGRTRYQHV TGTRCPTDFA EVPSILMEYF SNDYRVVSQF AKHYQTGQPL 550
    PKAMVSRLCE SKKVCAAAEM QLQVFYAALD QIYHGQHPLK KSTTDILMET 600
    QEQFYGLPYV PDTAWQLRFS HLVGYGAKYY SYLMSRAVAS MVWKECFLQD 650
    PFNRAAGERY RREMLAHGGG KEPMLMIQGM LQKCPSIDDF VDALVSDLNL 700
    DFETFFMDSK 710
    Length:710
    Mass (Da):80,674
    Last modified:October 1, 1993 - v1
    Checksum:i4FC2E6743EA43558
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96633 mRNA. Translation: AAA41899.1.
    PIRiA46273.
    RefSeqiNP_112314.1. NM_031052.1.
    UniGeneiRn.2266.

    Genome annotation databases

    GeneIDi81684.
    KEGGirno:81684.
    UCSCiRGD:621680. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96633 mRNA. Translation: AAA41899.1 .
    PIRi A46273.
    RefSeqi NP_112314.1. NM_031052.1.
    UniGenei Rn.2266.

    3D structure databases

    ProteinModelPortali Q01992.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000062641.

    Protein family/group databases

    MEROPSi M03.A05.

    PTM databases

    PhosphoSitei Q01992.

    Proteomic databases

    PaxDbi Q01992.
    PRIDEi Q01992.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81684.
    KEGGi rno:81684.
    UCSCi RGD:621680. rat.

    Organism-specific databases

    CTDi 4285.
    RGDi 621680. Mipep.

    Phylogenomic databases

    eggNOGi COG0339.
    HOGENOMi HOG000230535.
    HOVERGENi HBG008215.
    InParanoidi Q01992.
    KOi K01410.

    Miscellaneous databases

    NextBioi 615312.
    PROi Q01992.

    Gene expression databases

    Genevestigatori Q01992.

    Family and domain databases

    Gene3Di 1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProi IPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view ]
    Pfami PF01432. Peptidase_M3. 1 hit.
    [Graphical view ]
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of rat mitochondrial intermediate peptidase: similarity to zinc metallopeptidases and to a putative yeast homologue."
      Isaya G., Kalousek F., Rosenberg L.E.
      Proc. Natl. Acad. Sci. U.S.A. 89:8317-8321(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Rat liver mitochondrial intermediate peptidase (MIP): purification and initial characterization."
      Kalousek F., Isaya G., Rosenberg L.E.
      EMBO J. 11:2803-2809(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 34-50 AND 528-542.
      Tissue: Liver.

    Entry informationi

    Entry nameiMIPEP_RAT
    AccessioniPrimary (citable) accession number: Q01992
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3