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Q01992

- MIPEP_RAT

UniProt

Q01992 - MIPEP_RAT

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Protein

Mitochondrial intermediate peptidase

Gene

Mipep

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size.

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Enzyme regulationi

Activity is divalent cation-dependent. It is stimulated by manganese, magnesium or calcium ions and reversibly inhibited by zinc, cobalt and iron.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi492 – 4921Zinc; catalyticPROSITE-ProRule annotation
Active sitei493 – 4931PROSITE-ProRule annotation
Metal bindingi496 – 4961Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi499 – 4991Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Cobalt, Iron, Magnesium, Manganese, Metal-binding, Zinc

Protein family/group databases

MEROPSiM03.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Gene namesi
Name:Mipep
Synonyms:Mip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621680. Mipep.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion1 PublicationAdd
BLAST
Chaini34 – 710677Mitochondrial intermediate peptidasePRO_0000028580Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei124 – 1241N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ01992.
PRIDEiQ01992.

PTM databases

PhosphoSiteiQ01992.

Expressioni

Gene expression databases

GenevestigatoriQ01992.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062641.

Structurei

3D structure databases

ProteinModelPortaliQ01992.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0339.
HOGENOMiHOG000230535.
HOVERGENiHBG008215.
InParanoidiQ01992.
KOiK01410.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01992-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLAAGTRYA YRLCGRRAAA ALQGRAGRSC ARSVSTSWSP VGAAFNVKPQ
60 70 80 90 100
GHLWDLLGER RGLFGVPELS TPEGFQVAQE EALRKTEWLV ERACSTPPGP
110 120 130 140 150
QTVLIFDELS DCLCRVADLA DFVKIGHPEQ AFREAAQEAC RSIGTMVEKL
160 170 180 190 200
NTNVELYQSL QKLLDDKKLM DSLDAETRRV AELFMFDFEI SGIHLDEEKR
210 220 230 240 250
RRAVDLNVKI LDLSSAFLMG TNFPIKIQKH LLPEHIQHHF ARDGRHLVID
260 270 280 290 300
GLHAEASDDL VREAAYKIFL YPNADQLKCL EELLSSRDLL ANLVGYLPFP
310 320 330 340 350
TGPPGTIAQT PETVMQFLEK LSEKLCERTR KDFEMMQGMK TKLNPQNSEL
360 370 380 390 400
MPWDPPYYSG VIRAERYNIE PSLYCPFLSL GACMEGLNVL FNRLLGVTLY
410 420 430 440 450
AEQPFKGEVW CIDVRKLAVV HESEGLLGYI YCDFFQRANK PQQDCHFTIR
460 470 480 490 500
GGRLKEDGSY QLPVVVLMLN LPHASRDFPT LLTPGMMENL FHEMGHAMHS
510 520 530 540 550
MLGRTRYQHV TGTRCPTDFA EVPSILMEYF SNDYRVVSQF AKHYQTGQPL
560 570 580 590 600
PKAMVSRLCE SKKVCAAAEM QLQVFYAALD QIYHGQHPLK KSTTDILMET
610 620 630 640 650
QEQFYGLPYV PDTAWQLRFS HLVGYGAKYY SYLMSRAVAS MVWKECFLQD
660 670 680 690 700
PFNRAAGERY RREMLAHGGG KEPMLMIQGM LQKCPSIDDF VDALVSDLNL
710
DFETFFMDSK
Length:710
Mass (Da):80,674
Last modified:October 1, 1993 - v1
Checksum:i4FC2E6743EA43558
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96633 mRNA. Translation: AAA41899.1.
PIRiA46273.
RefSeqiNP_112314.1. NM_031052.1.
UniGeneiRn.2266.

Genome annotation databases

GeneIDi81684.
KEGGirno:81684.
UCSCiRGD:621680. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96633 mRNA. Translation: AAA41899.1 .
PIRi A46273.
RefSeqi NP_112314.1. NM_031052.1.
UniGenei Rn.2266.

3D structure databases

ProteinModelPortali Q01992.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000062641.

Protein family/group databases

MEROPSi M03.006.

PTM databases

PhosphoSitei Q01992.

Proteomic databases

PaxDbi Q01992.
PRIDEi Q01992.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 81684.
KEGGi rno:81684.
UCSCi RGD:621680. rat.

Organism-specific databases

CTDi 4285.
RGDi 621680. Mipep.

Phylogenomic databases

eggNOGi COG0339.
HOGENOMi HOG000230535.
HOVERGENi HBG008215.
InParanoidi Q01992.
KOi K01410.

Miscellaneous databases

NextBioi 615312.
PROi Q01992.

Gene expression databases

Genevestigatori Q01992.

Family and domain databases

Gene3Di 1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view ]
Pfami PF01432. Peptidase_M3. 1 hit.
[Graphical view ]
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of rat mitochondrial intermediate peptidase: similarity to zinc metallopeptidases and to a putative yeast homologue."
    Isaya G., Kalousek F., Rosenberg L.E.
    Proc. Natl. Acad. Sci. U.S.A. 89:8317-8321(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Rat liver mitochondrial intermediate peptidase (MIP): purification and initial characterization."
    Kalousek F., Isaya G., Rosenberg L.E.
    EMBO J. 11:2803-2809(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 34-50 AND 528-542.
    Tissue: Liver.

Entry informationi

Entry nameiMIPEP_RAT
AccessioniPrimary (citable) accession number: Q01992
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3