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Protein

Mitochondrial intermediate peptidase

Gene

Mipep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size.

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Enzyme regulationi

Activity is divalent cation-dependent. It is stimulated by manganese, magnesium or calcium ions and reversibly inhibited by zinc, cobalt and iron.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi492Zinc; catalyticPROSITE-ProRule annotation1
Active sitei493PROSITE-ProRule annotation1
Metal bindingi496Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi499Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • endopeptidase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandCalcium, Cobalt, Iron, Magnesium, Manganese, Metal-binding, Zinc

Protein family/group databases

MEROPSiM03.006

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Gene namesi
Name:Mipep
Synonyms:Mip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621680 Mipep

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33Mitochondrion1 PublicationAdd BLAST33
ChainiPRO_000002858034 – 710Mitochondrial intermediate peptidaseAdd BLAST677

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei124N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ01992
PRIDEiQ01992

PTM databases

iPTMnetiQ01992
PhosphoSitePlusiQ01992

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018845

Structurei

3D structure databases

ProteinModelPortaliQ01992
SMRiQ01992
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2090 Eukaryota
COG0339 LUCA
HOGENOMiHOG000230535
HOVERGENiHBG008215
InParanoidiQ01992

Family and domain databases

CDDicd06457 M3A_MIP, 1 hit
Gene3Di1.10.1370.10, 2 hits
InterProiView protein in InterPro
IPR033851 M3A_MIP
IPR024077 Neurolysin/TOP_dom2
IPR001567 Pept_M3A_M3B
PANTHERiPTHR11804:SF5 PTHR11804:SF5, 1 hit
PfamiView protein in Pfam
PF01432 Peptidase_M3, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLAAGTRYA YRLCGRRAAA ALQGRAGRSC ARSVSTSWSP VGAAFNVKPQ
60 70 80 90 100
GHLWDLLGER RGLFGVPELS TPEGFQVAQE EALRKTEWLV ERACSTPPGP
110 120 130 140 150
QTVLIFDELS DCLCRVADLA DFVKIGHPEQ AFREAAQEAC RSIGTMVEKL
160 170 180 190 200
NTNVELYQSL QKLLDDKKLM DSLDAETRRV AELFMFDFEI SGIHLDEEKR
210 220 230 240 250
RRAVDLNVKI LDLSSAFLMG TNFPIKIQKH LLPEHIQHHF ARDGRHLVID
260 270 280 290 300
GLHAEASDDL VREAAYKIFL YPNADQLKCL EELLSSRDLL ANLVGYLPFP
310 320 330 340 350
TGPPGTIAQT PETVMQFLEK LSEKLCERTR KDFEMMQGMK TKLNPQNSEL
360 370 380 390 400
MPWDPPYYSG VIRAERYNIE PSLYCPFLSL GACMEGLNVL FNRLLGVTLY
410 420 430 440 450
AEQPFKGEVW CIDVRKLAVV HESEGLLGYI YCDFFQRANK PQQDCHFTIR
460 470 480 490 500
GGRLKEDGSY QLPVVVLMLN LPHASRDFPT LLTPGMMENL FHEMGHAMHS
510 520 530 540 550
MLGRTRYQHV TGTRCPTDFA EVPSILMEYF SNDYRVVSQF AKHYQTGQPL
560 570 580 590 600
PKAMVSRLCE SKKVCAAAEM QLQVFYAALD QIYHGQHPLK KSTTDILMET
610 620 630 640 650
QEQFYGLPYV PDTAWQLRFS HLVGYGAKYY SYLMSRAVAS MVWKECFLQD
660 670 680 690 700
PFNRAAGERY RREMLAHGGG KEPMLMIQGM LQKCPSIDDF VDALVSDLNL
710
DFETFFMDSK
Length:710
Mass (Da):80,674
Last modified:October 1, 1993 - v1
Checksum:i4FC2E6743EA43558
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96633 mRNA Translation: AAA41899.1
PIRiA46273
UniGeneiRn.2266

Genome annotation databases

UCSCiRGD:621680 rat

Similar proteinsi

Entry informationi

Entry nameiMIPEP_RAT
AccessioniPrimary (citable) accession number: Q01992
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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