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Q01992 (MIPEP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial intermediate peptidase

Short name=MIP
EC=3.4.24.59
Gene names
Name:Mipep
Synonyms:Mip
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length710 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves proteins, imported into the mitochondrion, to their mature size.

Catalytic activity

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactor

Binds 1 zinc ion By similarity.

Enzyme regulation

Activity is divalent cation-dependent. It is stimulated by manganese, magnesium or calcium ions and reversibly inhibited by zinc, cobalt and iron.

Subunit structure

Monomer.

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the peptidase M3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3333Mitochondrion Ref.2
Chain34 – 710677Mitochondrial intermediate peptidase
PRO_0000028580

Sites

Active site4931 By similarity
Metal binding4921Zinc; catalytic By similarity
Metal binding4961Zinc; catalytic By similarity
Metal binding4991Zinc; catalytic By similarity

Amino acid modifications

Modified residue1241N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q01992 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 4FC2E6743EA43558

FASTA71080,674
        10         20         30         40         50         60 
MLLAAGTRYA YRLCGRRAAA ALQGRAGRSC ARSVSTSWSP VGAAFNVKPQ GHLWDLLGER 

        70         80         90        100        110        120 
RGLFGVPELS TPEGFQVAQE EALRKTEWLV ERACSTPPGP QTVLIFDELS DCLCRVADLA 

       130        140        150        160        170        180 
DFVKIGHPEQ AFREAAQEAC RSIGTMVEKL NTNVELYQSL QKLLDDKKLM DSLDAETRRV 

       190        200        210        220        230        240 
AELFMFDFEI SGIHLDEEKR RRAVDLNVKI LDLSSAFLMG TNFPIKIQKH LLPEHIQHHF 

       250        260        270        280        290        300 
ARDGRHLVID GLHAEASDDL VREAAYKIFL YPNADQLKCL EELLSSRDLL ANLVGYLPFP 

       310        320        330        340        350        360 
TGPPGTIAQT PETVMQFLEK LSEKLCERTR KDFEMMQGMK TKLNPQNSEL MPWDPPYYSG 

       370        380        390        400        410        420 
VIRAERYNIE PSLYCPFLSL GACMEGLNVL FNRLLGVTLY AEQPFKGEVW CIDVRKLAVV 

       430        440        450        460        470        480 
HESEGLLGYI YCDFFQRANK PQQDCHFTIR GGRLKEDGSY QLPVVVLMLN LPHASRDFPT 

       490        500        510        520        530        540 
LLTPGMMENL FHEMGHAMHS MLGRTRYQHV TGTRCPTDFA EVPSILMEYF SNDYRVVSQF 

       550        560        570        580        590        600 
AKHYQTGQPL PKAMVSRLCE SKKVCAAAEM QLQVFYAALD QIYHGQHPLK KSTTDILMET 

       610        620        630        640        650        660 
QEQFYGLPYV PDTAWQLRFS HLVGYGAKYY SYLMSRAVAS MVWKECFLQD PFNRAAGERY 

       670        680        690        700        710 
RREMLAHGGG KEPMLMIQGM LQKCPSIDDF VDALVSDLNL DFETFFMDSK 

« Hide

References

[1]"Sequence analysis of rat mitochondrial intermediate peptidase: similarity to zinc metallopeptidases and to a putative yeast homologue."
Isaya G., Kalousek F., Rosenberg L.E.
Proc. Natl. Acad. Sci. U.S.A. 89:8317-8321(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Rat liver mitochondrial intermediate peptidase (MIP): purification and initial characterization."
Kalousek F., Isaya G., Rosenberg L.E.
EMBO J. 11:2803-2809(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 34-50 AND 528-542.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96633 mRNA. Translation: AAA41899.1.
PIRA46273.
RefSeqNP_112314.1. NM_031052.1.
UniGeneRn.2266.

3D structure databases

ProteinModelPortalQ01992.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000062641.

Protein family/group databases

MEROPSM03.A05.

PTM databases

PhosphoSiteQ01992.

Proteomic databases

PaxDbQ01992.
PRIDEQ01992.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID81684.
KEGGrno:81684.
UCSCRGD:621680. rat.

Organism-specific databases

CTD4285.
RGD621680. Mipep.

Phylogenomic databases

eggNOGCOG0339.
HOGENOMHOG000230535.
HOVERGENHBG008215.
InParanoidQ01992.
KOK01410.

Gene expression databases

GenevestigatorQ01992.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615312.
PROQ01992.

Entry information

Entry nameMIPEP_RAT
AccessionPrimary (citable) accession number: Q01992
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries