Q01992 (MIPEP_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial intermediate peptidase
Short name=MIP
EC=3.4.24.59

Gene names

Name:	Mipep
Synonyms:	Mip

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	710 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Enzyme regulation	Activity is divalent cation-dependent. It is stimulated by manganese, magnesium or calcium ions and reversibly inhibited by zinc, cobalt and iron.
Subunit structure	Monomer.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Calcium Cobalt Iron Magnesium Manganese Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion Ref.2
	Chain	34 – 710	677	Mitochondrial intermediate peptidase		PRO_0000028580
Sites
	Active site	493	1	By similarity
	Metal binding	492	1	Zinc; catalytic By similarity
	Metal binding	496	1	Zinc; catalytic By similarity
	Metal binding	499	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	124	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01992 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 4FC2E6743EA43558	FASTA	710	80,674
	10 20 30 40 50 60 MLLAAGTRYA YRLCGRRAAA ALQGRAGRSC ARSVSTSWSP VGAAFNVKPQ GHLWDLLGER 70 80 90 100 110 120 RGLFGVPELS TPEGFQVAQE EALRKTEWLV ERACSTPPGP QTVLIFDELS DCLCRVADLA 130 140 150 160 170 180 DFVKIGHPEQ AFREAAQEAC RSIGTMVEKL NTNVELYQSL QKLLDDKKLM DSLDAETRRV 190 200 210 220 230 240 AELFMFDFEI SGIHLDEEKR RRAVDLNVKI LDLSSAFLMG TNFPIKIQKH LLPEHIQHHF 250 260 270 280 290 300 ARDGRHLVID GLHAEASDDL VREAAYKIFL YPNADQLKCL EELLSSRDLL ANLVGYLPFP 310 320 330 340 350 360 TGPPGTIAQT PETVMQFLEK LSEKLCERTR KDFEMMQGMK TKLNPQNSEL MPWDPPYYSG 370 380 390 400 410 420 VIRAERYNIE PSLYCPFLSL GACMEGLNVL FNRLLGVTLY AEQPFKGEVW CIDVRKLAVV 430 440 450 460 470 480 HESEGLLGYI YCDFFQRANK PQQDCHFTIR GGRLKEDGSY QLPVVVLMLN LPHASRDFPT 490 500 510 520 530 540 LLTPGMMENL FHEMGHAMHS MLGRTRYQHV TGTRCPTDFA EVPSILMEYF SNDYRVVSQF 550 560 570 580 590 600 AKHYQTGQPL PKAMVSRLCE SKKVCAAAEM QLQVFYAALD QIYHGQHPLK KSTTDILMET 610 620 630 640 650 660 QEQFYGLPYV PDTAWQLRFS HLVGYGAKYY SYLMSRAVAS MVWKECFLQD PFNRAAGERY 670 680 690 700 710 RREMLAHGGG KEPMLMIQGM LQKCPSIDDF VDALVSDLNL DFETFFMDSK

References

[1]	"Sequence analysis of rat mitochondrial intermediate peptidase: similarity to zinc metallopeptidases and to a putative yeast homologue." Isaya G., Kalousek F., Rosenberg L.E. Proc. Natl. Acad. Sci. U.S.A. 89:8317-8321(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Rat liver mitochondrial intermediate peptidase (MIP): purification and initial characterization." Kalousek F., Isaya G., Rosenberg L.E. EMBO J. 11:2803-2809(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 34-50 AND 528-542. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96633 mRNA. Translation: AAA41899.1.
IPI	IPI00326901.
PIR	A46273.
RefSeq	NP_112314.1. NM_031052.1.
UniGene	Rn.2266.
3D structure databases
ProteinModelPortal	Q01992.
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
PTM databases
PhosphoSite	Q01992.
Proteomic databases
PaxDb	Q01992.
PRIDE	Q01992.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81684.
KEGG	rno:81684.
UCSC	RGD:621680. rat.
Organism-specific databases
CTD	4285.
RGD	621680. Mipep.
Phylogenomic databases
eggNOG	COG0339.
HOGENOM	HOG000230535.
HOVERGEN	HBG008215.
InParanoid	Q01992.
KO	K01410.
Gene expression databases
ArrayExpress	Q01992.
Genevestigator	Q01992.
GermOnline	ENSRNOG00000013876. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.1370.10. 2 hits. 1.20.1050.40. 1 hit. 3.40.390.10. 1 hit.
InterPro	IPR024079. MetalloPept_cat_dom. IPR024077. Neurolysin/TOP_dom2. IPR024080. Neurolysin/TOP_N. IPR001567. Pept_M3A_M3B. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615312.

Entry information

Entry name

MIPEP_RAT

Accession

Primary (citable) accession number: Q01992

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program