Histamine N-methyltransferase - Rattus norvegicus (Rat)

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Q01984 (HNMT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Histamine N-methyltransferase
Short name=HMT
EC=2.1.1.8

Gene names

Name:

Hnmt

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
Catalytic activity	S-adenosyl-L-methionine + histamine = S-adenosyl-L-homocysteine + N(tau)-methylhistamine.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the methyltransferase superfamily. HNMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from expression pattern PubMed 971743. Source: RGD hyperosmotic response Inferred from expression pattern PubMed 2429527. Source: RGD response to amine stimulus Inferred from expression pattern PubMed 3952132. Source: RGD response to cocaine Inferred from expression pattern PubMed 9231750. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern PubMed 2717067. Source: RGD response to interleukin-1 Inferred from expression pattern PubMed 8594930. Source: RGD response to tumor cell Inferred from expression pattern PubMed 3111198. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell neuron projection Inferred from direct assay PubMed 2717067. Source: RGD nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	histamine N-methyltransferase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

Histamine N-methyltransferase

PRO_0000084024

Sites

Binding site

Substrate By similarity

Binding site

S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Binding site

S-adenosyl-L-methionine By similarity

Binding site

S-adenosyl-L-methionine By similarity

Binding site

120

S-adenosyl-L-methionine; via amide nitrogen By similarity

Binding site

143

S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Binding site

284

Substrate By similarity

Amino acid modifications

Modified residue

Blocked amino end (Ala)

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q01984 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A54539BA3AE1032E
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FASTA

295

33,942

        10         20         30         40         50         60 
MASFMRSLFS DHSRYVESFR RFLNNSTEHQ CMQEFMDKKL PGIIARIGET KAEIKILSIG 

        70         80         90        100        110        120 
GGAGEIDLQI LSKVQAQYPG ICINNEVVEP NAEQIVKYKE LVAKTSNMEN IKFAWHKETS 

       130        140        150        160        170        180 
SEYQKRVVEE DEEPPKWDFI HMIQMLYYVK DIPATLKFFH GLLAANAKIL IILVSGTSGW 

       190        200        210        220        230        240 
EKLWKKYGFR LPRDDLCQYV TSSDLAQILD DLGIKYECYD LLSTMDITDC FIDGNENGDL 

       250        260        270        280        290 
LWDFLTETCN FIKTAPLDLK EEIMKDLQEP EFSVKKEGKV LFNNNLSFIV VEANV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histamine N-methyltransferase from rat kidney. Cloning, nucleotide sequence, and expression in Escherichia coli cells." Takemura M., Tanaka T., Taguchi Y., Imamura I., Mizuguchi H., Kuroda M., Fukui H., Yamatodani A., Wada H. J. Biol. Chem. 267:15687-15691(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Kidney.
[2]	"Genomic structure of the rat and mouse histamine N-methyltransferase gene." Kitanaka N., Kitanaka J., Oue T., Tada Y., Tanaka T., Takemura M. Jpn. J. Pharmacol. 88:85-92(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10693 mRNA. Translation: BAA01535.1. AB007834 Genomic DNA. Translation: BAB84319.1. BC087635 mRNA. Translation: AAH87635.1.
IPI	IPI00230903.
PIR	A42851.
RefSeq	NP_112306.1. NM_031044.3.
UniGene	Rn.13145.
3D structure databases
ProteinModelPortal	Q01984.
SMR	Q01984. Positions 5-293.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000007471.
Proteomic databases
PaxDb	Q01984.
PRIDE	Q01984.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000007471; ENSRNOP00000007471; ENSRNOG00000005223.
GeneID	81676.
KEGG	rno:81676.
UCSC	RGD:71049. rat.
Organism-specific databases
CTD	3176.
RGD	71049. Hnmt.
Phylogenomic databases
eggNOG	NOG39680.
GeneTree	ENSGT00390000002862.
HOGENOM	HOG000231790.
HOVERGEN	HBG051914.
InParanoid	Q01984.
KO	K00546.
OMA	WKKYGSR.
OrthoDB	EOG4NCMDJ.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14646.
Gene expression databases
Genevestigator	Q01984.
GermOnline	ENSRNOG00000005223. Rattus norvegicus.
Family and domain databases
InterPro	IPR016673. Histamine_N-methyltransferase. [Graphical view]
PANTHER	PTHR32330. PTHR32330. 1 hit.
PIRSF	PIRSF016616. HHMT. 1 hit.
ProtoNet	Search...
Other
BindingDB	Q01984.
ChEMBL	CHEMBL3241.
NextBio	615280.

Entry information

Entry name

HNMT_RAT

Accession

Primary (citable) accession number: Q01984
Secondary accession number(s): Q59JM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents