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Protein

Histamine N-methyltransferase

Gene

Hnmt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + histamine = S-adenosyl-L-homocysteine + N(tau)-methylhistamine.PROSITE-ProRule annotation1 Publication

Kineticsi

  1. KM=7.1 µM for histamine (at pH 7.5)1 Publication
  2. KM=6.3 µM for AdoMet (at pH 7.5)1 Publication
  1. Vmax=1.6 µmol/min/mg enzyme (at pH 8.5)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28SubstratePROSITE-ProRule annotation1
Binding sitei60S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei89S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei94S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei120S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei143S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei284SubstratePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • brain development Source: RGD
  • histamine catabolic process Source: UniProtKB
  • hyperosmotic response Source: RGD
  • positive regulation of protein targeting to mitochondrion Source: Ensembl
  • response to amine Source: RGD
  • response to cocaine Source: RGD
  • response to glucocorticoid Source: RGD
  • response to immobilization stress Source: RGD
  • response to interleukin-1 Source: RGD
  • response to tumor cell Source: RGD
  • selenium compound metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14646.
ReactomeiR-RNO-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.
R-RNO-70921. Histidine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Histamine N-methyltransferase (EC:2.1.1.81 Publication)
Short name:
HMT
Gene namesi
Name:Hnmt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi71049. Hnmt.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: Ensembl
  • neuron projection Source: RGD
  • nucleoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3241.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000840241 – 295Histamine N-methyltransferaseAdd BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Blocked amino end (Ala)1

Proteomic databases

PaxDbiQ01984.
PRIDEiQ01984.

PTM databases

iPTMnetiQ01984.
PhosphoSitePlusiQ01984.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005223.
GenevisibleiQ01984. RN.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007471.

Chemistry databases

BindingDBiQ01984.

Structurei

3D structure databases

ProteinModelPortaliQ01984.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. HNMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IGS0. Eukaryota.
ENOG410ZU1D. LUCA.
GeneTreeiENSGT00390000002862.
HOGENOMiHOG000231790.
HOVERGENiHBG051914.
InParanoidiQ01984.
KOiK00546.
OMAiMDISDCF.
OrthoDBiEOG091G0DY6.
PhylomeDBiQ01984.
TreeFamiTF331080.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016673. HHMT-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF016616. HHMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51597. SAM_HNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01984-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFMRSLFS DHSRYVESFR RFLNNSTEHQ CMQEFMDKKL PGIIARIGET
60 70 80 90 100
KAEIKILSIG GGAGEIDLQI LSKVQAQYPG ICINNEVVEP NAEQIVKYKE
110 120 130 140 150
LVAKTSNMEN IKFAWHKETS SEYQKRVVEE DEEPPKWDFI HMIQMLYYVK
160 170 180 190 200
DIPATLKFFH GLLAANAKIL IILVSGTSGW EKLWKKYGFR LPRDDLCQYV
210 220 230 240 250
TSSDLAQILD DLGIKYECYD LLSTMDITDC FIDGNENGDL LWDFLTETCN
260 270 280 290
FIKTAPLDLK EEIMKDLQEP EFSVKKEGKV LFNNNLSFIV VEANV
Length:295
Mass (Da):33,942
Last modified:January 23, 2007 - v3
Checksum:iA54539BA3AE1032E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10693 mRNA. Translation: BAA01535.1.
AB007834 Genomic DNA. Translation: BAB84319.1.
BC087635 mRNA. Translation: AAH87635.1.
PIRiA42851.
RefSeqiNP_112306.1. NM_031044.3.
UniGeneiRn.13145.

Genome annotation databases

EnsembliENSRNOT00000007471; ENSRNOP00000007471; ENSRNOG00000005223.
GeneIDi81676.
KEGGirno:81676.
UCSCiRGD:71049. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10693 mRNA. Translation: BAA01535.1.
AB007834 Genomic DNA. Translation: BAB84319.1.
BC087635 mRNA. Translation: AAH87635.1.
PIRiA42851.
RefSeqiNP_112306.1. NM_031044.3.
UniGeneiRn.13145.

3D structure databases

ProteinModelPortaliQ01984.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007471.

Chemistry databases

BindingDBiQ01984.
ChEMBLiCHEMBL3241.

PTM databases

iPTMnetiQ01984.
PhosphoSitePlusiQ01984.

Proteomic databases

PaxDbiQ01984.
PRIDEiQ01984.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007471; ENSRNOP00000007471; ENSRNOG00000005223.
GeneIDi81676.
KEGGirno:81676.
UCSCiRGD:71049. rat.

Organism-specific databases

CTDi3176.
RGDi71049. Hnmt.

Phylogenomic databases

eggNOGiENOG410IGS0. Eukaryota.
ENOG410ZU1D. LUCA.
GeneTreeiENSGT00390000002862.
HOGENOMiHOG000231790.
HOVERGENiHBG051914.
InParanoidiQ01984.
KOiK00546.
OMAiMDISDCF.
OrthoDBiEOG091G0DY6.
PhylomeDBiQ01984.
TreeFamiTF331080.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14646.
ReactomeiR-RNO-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.
R-RNO-70921. Histidine catabolism.

Miscellaneous databases

PROiQ01984.

Gene expression databases

BgeeiENSRNOG00000005223.
GenevisibleiQ01984. RN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016673. HHMT-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF016616. HHMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51597. SAM_HNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNMT_RAT
AccessioniPrimary (citable) accession number: Q01984
Secondary accession number(s): Q59JM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.