Q01976 (ADPP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribose pyrophosphatase
EC=3.6.1.13

Alternative name(s):
ADP-ribose diphosphatase
ADP-ribose phosphohydrolase
Adenosine diphosphoribose pyrophosphatase
Short name=ADPR-PPase

Gene names

Name:	YSA1
Ordered Locus Names:	YBR111C
ORF Names:	YBR0907

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate.
Cofactor	Binds 3 magnesium ions per subunit. Can also accept manganese ions By similarity.
Miscellaneous	Present with 7330 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the Nudix hydrolase family. NudF subfamily. Contains 1 nudix hydrolase domain.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ribose phosphate metabolic process Inferred from direct assay Ref.6. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ADP-ribose diphosphatase activity Inferred from direct assay Ref.6. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	ADP-ribose pyrophosphatase		PRO_0000057050
Regions
	Domain	75 – 214	140	Nudix hydrolase
	Region	64 – 65	2	Substrate binding; shared with dimeric partner By similarity
	Motif	116 – 137	22	Nudix box
Sites
	Metal binding	115	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	131	1	Magnesium 2 By similarity
	Metal binding	131	1	Magnesium 3 By similarity
	Metal binding	135	1	Magnesium 1 By similarity
	Metal binding	135	1	Magnesium 3 By similarity
	Metal binding	185	1	Magnesium 3 By similarity
	Binding site	46	1	Substrate By similarity
	Binding site	69	1	Substrate; shared with dimeric partner By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	117	1	Substrate; via amide nitrogen By similarity
	Binding site	152	1	Substrate By similarity
Amino acid modifications
	Modified residue	74	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q01976 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 512DE7446A39EFBA	FASTA	231	26,087
	10 20 30 40 50 60 MFLRNVRVIS LNSRRLFRTM STVKGKPEDA KIIEARHVKE TSDCKWIGLQ KIIYKDPNGK 70 80 90 100 110 120 EREWDSAVRT TRSSGGVDGI GILTILKYKD GKPDEILLQK QFRPPVEGVC IEMPAGLIDA 130 140 150 160 170 180 GEDIDTAALR ELKEETGYSG KIISKSPTVF NDPGFTNTNL CLVTVEVDMS LPENQKPVTQ 190 200 210 220 230 LEDNEFIECF SVELHKFPDE MVKLDQQGYK LDARVQNVAQ GILMAKQYHI K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Molecular analysis of yeast chromosome II between CMD1 and LYS2: the excision repair gene RAD16 located in this region belongs to a novel group of double-finger proteins." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 8:397-408(1992) [PubMed: 1626431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-47. Strain: ATCC 204508 / S288c.
[6]	"Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance." Dunn C.A., O'Handley S.F., Frick D.N., Bessman M.J. J. Biol. Chem. 274:32318-32324(1999) [PubMed: 10542272] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78993 Genomic DNA. Translation: CAA55614.1. Z35980 Genomic DNA. Translation: CAA85068.1. AY558185 Genomic DNA. Translation: AAS56511.1. X66247 Genomic DNA. Translation: CAA46972.1. BK006936 Genomic DNA. Translation: DAA07230.1.
PIR	S48276.
RefSeq	NP_009669.1. NM_001178459.1.
3D structure databases
ProteinModelPortal	Q01976.
SMR	Q01976. Positions 29-230.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q01976. 2 interactions.
STRING	Q01976.
Proteomic databases
PeptideAtlas	Q01976.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR111C; YBR111C; YBR111C.
GeneID	852408.
KEGG	sce:YBR111C.
NMPDR	fig|4932.3.peg.369.
Organism-specific databases
CYGD	YBR111c.
SGD	S000000315. YSA1.
Phylogenomic databases
eggNOG	fuNOG05093.
GeneTree	EFGT00050000006072.
HOGENOM	HBG446368.
OMA	GVCIEMP.
OrthoDB	EOG4J9R84.
Gene expression databases
ArrayExpress	Q01976.
Genevestigator	Q01976.
GermOnline	YBR111C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020084. NUDIX_hydrolase_CS. IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
KO	K01515.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
SUPFAM	SSF55811. NUDIX_hydrolase. 1 hit.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971255.

Entry information

Entry name

ADPP_YEAST

Accession

Primary (citable) accession number: Q01976
Secondary accession number(s): D6VQB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program