Reviewed, UniProtKB/Swiss-Prot Q01976 (ADPP_YEAST)

Names and origin

Protein names

Recommended name:
    ADP-ribose pyrophosphatase
    EC=3.6.1.13
Alternative name(s):
    ADP-ribose diphosphatase
    Adenosine diphosphoribose pyrophosphatase
      Short name=ADPR-PPase
    ADP-ribose phosphohydrolase

Gene names

Name:	YSA1
Ordered Locus Names:	YBR111C
ORF Names:	YBR0907

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate.
Cofactor	Divalent ions. Magnesium or manganese.
Miscellaneous	Present with 7330 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the Nudix hydrolase family. NudF subfamily.

Ontologies

Keywords
Ligand	Magnesium Manganese
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ribose phosphate metabolic process Ref.5 Inferred from direct assay. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ADP-ribose diphosphatase activity Ref.5 Inferred from direct assay. Source: SGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	ADP-ribose pyrophosphatase		PRO_0000057050
Regions
	Motif	116 – 137	22	Nudix box
Amino acid modifications
	Modified residue	74	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q01976-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 512DE7446A39EFBA	FASTA	231	26,087
	10 20 30 40 50 60 MFLRNVRVIS LNSRRLFRTM STVKGKPEDA KIIEARHVKE TSDCKWIGLQ KIIYKDPNGK 70 80 90 100 110 120 EREWDSAVRT TRSSGGVDGI GILTILKYKD GKPDEILLQK QFRPPVEGVC IEMPAGLIDA 130 140 150 160 170 180 GEDIDTAALR ELKEETGYSG KIISKSPTVF NDPGFTNTNL CLVTVEVDMS LPENQKPVTQ 190 200 210 220 230 LEDNEFIECF SVELHKFPDE MVKLDQQGYK LDARVQNVAQ GILMAKQYHI K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Molecular analysis of yeast chromosome II between CMD1 and LYS2: the excision repair gene RAD16 located in this region belongs to a novel group of double-finger proteins." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 8:397-408(1992) [PubMed: 1626431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-47. Strain: ATCC 204508 / S288c.
[5]	"Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance." Dunn C.A., O'Handley S.F., Frick D.N., Bessman M.J. J. Biol. Chem. 274:32318-32324(1999) [PubMed: 10542272] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78993 Genomic DNA. Translation: CAA55614.1. Z35980 Genomic DNA. Translation: CAA85068.1. AY558185 Genomic DNA. Translation: AAS56511.1. X66247 Genomic DNA. Translation: CAA46972.1.
PIR	S48276.
RefSeq	NP_009669.1.
3D structure databases
ModBase	Search...
Proteomic databases
PeptideAtlas	Q01976.
Genome annotation databases
Ensembl	YBR111C. Saccharomyces cerevisiae. [Contig view]
GeneID	852408.
GenomeReviews	Gene locus YBR111C in contig Y13134_GR.
KEGG	sce:YBR111C.
NMPDR	fig|4932.3.peg.369.
Organism-specific databases
CYGD	YBR111c.
SGD	S000000315. YSA1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q01976.
OMA	Q01976. VCIEMPA.
Enzyme and pathway databases
BRENDA	3.6.1.13. 250.
Gene expression databases
GermOnline	YBR111C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971255.

Entry information

Entry name

ADPP_YEAST

Accession

Primary (citable) accession number: Q01976

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)