Q01973 (ROR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase transmembrane receptor ROR1
EC=2.7.10.1

Alternative name(s):
Neurotrophic tyrosine kinase, receptor-related 1

Gene names

Name:	ROR1
Synonyms:	NTRKR1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	937 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tyrosine-protein kinase receptor whose role is not yet clear.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.
Developmental stage	Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. Contains 1 FZ (frizzled) domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 kringle domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Kringle Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transmembrane receptor protein tyrosine kinase signaling pathway Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Traceable author statement Ref.2. Source: ProtInc integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane receptor protein tyrosine kinase activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EGFR	P00533	8	EBI-6082337,EBI-297353
SRC	P12931	9	EBI-6082337,EBI-621482
Src	P05480	3	EBI-6082337,EBI-298680	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q01973-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q01973-2)

Also known as: T-ROR1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-549: Missing.

Isoform 3 (identifier: Q01973-3)

The sequence of this isoform differs from the canonical sequence as follows:
     392-393: DS → GK
     394-937: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 937	908	Tyrosine-protein kinase transmembrane receptor ROR1		PRO_0000024458
Regions
	Topological domain	30 – 406	377	Extracellular Potential
	Transmembrane	407 – 427	21	Helical; Potential
	Topological domain	428 – 937	510	Cytoplasmic Potential
	Domain	42 – 147	106	Ig-like C2-type
	Domain	165 – 299	135	FZ
	Domain	312 – 391	80	Kringle
	Domain	473 – 746	274	Protein kinase
	Nucleotide binding	479 – 487	9	ATP By similarity
	Compositional bias	753 – 782	30	Ser/Thr-rich
	Compositional bias	784 – 851	68	Pro-rich
	Compositional bias	853 – 876	24	Ser/Thr-rich
Sites
	Active site	615	1	Proton acceptor By similarity
	Binding site	506	1	ATP By similarity
Amino acid modifications
	Modified residue	645	1	Phosphotyrosine; by autocatalysis By similarity
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Disulfide bond	79 ↔ 131		By similarity
	Disulfide bond	170 ↔ 235		By similarity
	Disulfide bond	178 ↔ 228		By similarity
	Disulfide bond	219 ↔ 260		By similarity
	Disulfide bond	248 ↔ 296		By similarity
	Disulfide bond	252 ↔ 282		By similarity
	Disulfide bond	313 ↔ 391		By similarity
	Disulfide bond	334 ↔ 374		By similarity
	Disulfide bond	362 ↔ 386		By similarity
Natural variations
	Alternative sequence	1 – 549	549	Missing in isoform Short.		VSP_005008
	Alternative sequence	392 – 393	2	DS → GK in isoform 3.		VSP_043663
	Alternative sequence	394 – 937	544	Missing in isoform 3.		VSP_043664
	Natural variant	144	1	G → E in a metastatic melanoma sample; somatic mutation. Ref.7		VAR_041779
	Natural variant	150	1	F → L in an ovarian mucinous carcinoma sample; somatic mutation. Ref.7		VAR_041780
	Natural variant	301	1	I → V in a renal clear cell carcinoma sample; somatic mutation. Ref.7		VAR_041781
	Natural variant	518	1	T → M. Ref.1 Ref.7 Corresponds to variant rs7527017 [ dbSNP | Ensembl ].		VAR_041782
	Natural variant	562	1	E → D in a breast cancer sample; somatic mutation. Ref.6		VAR_035713
	Natural variant	567	1	R → I in a colorectal adenocarcinoma sample; somatic mutation. Ref.7		VAR_041783
	Natural variant	624	1	G → R. Ref.7 Corresponds to variant rs55832740 [ dbSNP | Ensembl ].		VAR_041784
	Natural variant	646	1	Y → C. Ref.7 Corresponds to variant rs34109134 [ dbSNP | Ensembl ].		VAR_041785
	Natural variant	776	1	S → N in a colorectal adenocarcinoma sample; somatic mutation. Ref.7		VAR_041786

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 0EC0E4762A90C6C7	FASTA	937	104,283
	10 20 30 40 50 60 MHRPRRRGTR PPLLALLAAL LLAARGAAAQ ETELSVSAEL VPTSSWNISS ELNKDSYLTL 70 80 90 100 110 120 DEPMNNITTS LGQTAELHCK VSGNPPPTIR WFKNDAPVVQ EPRRLSFRST IYGSRLRIRN 130 140 150 160 170 180 LDTTDTGYFQ CVATNGKEVV SSTGVLFVKF GPPPTASPGY SDEYEEDGFC QPYRGIACAR 190 200 210 220 230 240 FIGNRTVYME SLHMQGEIEN QITAAFTMIG TSSHLSDKCS QFAIPSLCHY AFPYCDETSS 250 260 270 280 290 300 VPKPRDLCRD ECEILENVLC QTEYIFARSN PMILMRLKLP NCEDLPQPES PEAANCIRIG 310 320 330 340 350 360 IPMADPINKN HKCYNSTGVD YRGTVSVTKS GRQCQPWNSQ YPHTHTFTAL RFPELNGGHS 370 380 390 400 410 420 YCRNPGNQKE APWCFTLDEN FKSDLCDIPA CDSKDSKEKN KMEILYILVP SVAIPLAIAL 430 440 450 460 470 480 LFFFICVCRN NQKSSSAPVQ RQPKHVRGQN VEMSMLNAYK PKSKAKELPL SAVRFMEELG 490 500 510 520 530 540 ECAFGKIYKG HLYLPGMDHA QLVAIKTLKD YNNPQQWTEF QQEASLMAEL HHPNIVCLLG 550 560 570 580 590 600 AVTQEQPVCM LFEYINQGDL HEFLIMRSPH SDVGCSSDED GTVKSSLDHG DFLHIAIQIA 610 620 630 640 650 660 AGMEYLSSHF FVHKDLAARN ILIGEQLHVK ISDLGLSREI YSADYYRVQS KSLLPIRWMP 670 680 690 700 710 720 PEAIMYGKFS SDSDIWSFGV VLWEIFSFGL QPYYGFSNQE VIEMVRKRQL LPCSEDCPPR 730 740 750 760 770 780 MYSLMTECWN EIPSRRPRFK DIHVRLRSWE GLSSHTSSTT PSGGNATTQT TSLSASPVSN 790 800 810 820 830 840 LSNPRYPNYM FPSQGITPQG QIAGFIGPPI PQNQRFIPIN GYPIPPGYAA FPAAHYQPTG 850 860 870 880 890 900 PPRVIQHCPP PKSRSPSSAS GSTSTGHVTS LPSSGSNQEA NIPLLPHMSI PNHPGGMGIT 910 920 930 VFGNKSQKPY KIDSKQASLL GDANIHGHTE SMISAEL
	Isoform Short (T-ROR1) [UniParc]. Checksum: 8DC1D0BA14085BD2	FASTA	388	42,833
	10 20 30 40 50 60 MLFEYINQGD LHEFLIMRSP HSDVGCSSDE DGTVKSSLDH GDFLHIAIQI AAGMEYLSSH 70 80 90 100 110 120 FFVHKDLAAR NILIGEQLHV KISDLGLSRE IYSADYYRVQ SKSLLPIRWM PPEAIMYGKF 130 140 150 160 170 180 SSDSDIWSFG VVLWEIFSFG LQPYYGFSNQ EVIEMVRKRQ LLPCSEDCPP RMYSLMTECW 190 200 210 220 230 240 NEIPSRRPRF KDIHVRLRSW EGLSSHTSST TPSGGNATTQ TTSLSASPVS NLSNPRYPNY 250 260 270 280 290 300 MFPSQGITPQ GQIAGFIGPP IPQNQRFIPI NGYPIPPGYA AFPAAHYQPT GPPRVIQHCP 310 320 330 340 350 360 PPKSRSPSSA SGSTSTGHVT SLPSSGSNQE ANIPLLPHMS IPNHPGGMGI TVFGNKSQKP 370 380 YKIDSKQASL LGDANIHGHT ESMISAEL
	Isoform 3 [UniParc]. Checksum: 1F93DCBBBBF53855	FASTA	393	43,826
	10 20 30 40 50 60 MHRPRRRGTR PPLLALLAAL LLAARGAAAQ ETELSVSAEL VPTSSWNISS ELNKDSYLTL 70 80 90 100 110 120 DEPMNNITTS LGQTAELHCK VSGNPPPTIR WFKNDAPVVQ EPRRLSFRST IYGSRLRIRN 130 140 150 160 170 180 LDTTDTGYFQ CVATNGKEVV SSTGVLFVKF GPPPTASPGY SDEYEEDGFC QPYRGIACAR 190 200 210 220 230 240 FIGNRTVYME SLHMQGEIEN QITAAFTMIG TSSHLSDKCS QFAIPSLCHY AFPYCDETSS 250 260 270 280 290 300 VPKPRDLCRD ECEILENVLC QTEYIFARSN PMILMRLKLP NCEDLPQPES PEAANCIRIG 310 320 330 340 350 360 IPMADPINKN HKCYNSTGVD YRGTVSVTKS GRQCQPWNSQ YPHTHTFTAL RFPELNGGHS 370 380 390 YCRNPGNQKE APWCFTLDEN FKSDLCDIPA CGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel family of cell surface receptors with tyrosine kinase-like domain." Masiakowski P., Carroll R.D. J. Biol. Chem. 267:26181-26190(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), VARIANT MET-518.
[2]	"Human neural tissues express a truncated Ror1 receptor tyrosine kinase, lacking both extracellular and transmembrane domains." Reddy U.R., Phatak S., Pleasure D. Oncogene 13:1555-1559(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ASP-562.
[7]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLU-144; LEU-150; VAL-301; MET-518; ILE-567; ARG-624; CYS-646 AND ASN-776.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M97675 mRNA. Translation: AAA60275.1. U38894 mRNA. Translation: AAC50714.1. AL137859, AL161742, AL445205 Genomic DNA. Translation: CAC17591.2. AL445205, AL137859, AL161742 Genomic DNA. Translation: CAH71705.1. AL445205, AL137859, AL161742 Genomic DNA. Translation: CAH71706.1. AL137859, AL445205, AL161742 Genomic DNA. Translation: CAI21732.1. AL161742, AL137859, AL445205 Genomic DNA. Translation: CAI21931.1. AL161742, AL445205, AL137859 Genomic DNA. Translation: CAI21932.1. AL138793 Genomic DNA. No translation available. AL353713 Genomic DNA. No translation available. AL808029 Genomic DNA. No translation available. AL808030 Genomic DNA. No translation available. CH471059 Genomic DNA. Translation: EAX06556.1. BC080541 mRNA. Translation: AAH80541.1.
IPI	IPI00010405. IPI00472950. IPI00644416.
PIR	A45082.
RefSeq	NP_001077061.1. NM_001083592.1. NP_005003.2. NM_005012.3.
UniGene	Hs.128753.
3D structure databases
ProteinModelPortal	Q01973.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29734N. DIP-5713N.
IntAct	Q01973. 8 interactions.
STRING	9606.ENSP00000360120.
PTM databases
PhosphoSite	Q01973.
Polymorphism databases
DMDM	118572711.
Proteomic databases
PaxDb	Q01973.
PRIDE	Q01973.
Protocols and materials databases
DNASU	4919.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371079; ENSP00000360120; ENSG00000185483. ENST00000371080; ENSP00000360121; ENSG00000185483. ENST00000545203; ENSP00000441637; ENSG00000185483.
GeneID	4919.
KEGG	hsa:4919.
UCSC	uc001dbj.2. human.
Organism-specific databases
CTD	4919.
GeneCards	GC01P064239.
HGNC	HGNC:10256. ROR1.
MIM	602336. gene.
neXtProt	NX_Q01973.
PharmGKB	PA34628.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOVERGEN	HBG017736.
InParanoid	Q01973.
KO	K05122.
OrthoDB	EOG4XGZZC.
PhylomeDB	Q01973.
Enzyme and pathway databases
BRENDA	2.7.10.1. 2681.
Gene expression databases
Bgee	Q01973.
CleanEx	HS_ROR1.
Genevestigator	Q01973.
GermOnline	ENSG00000185483. Homo sapiens.
Family and domain databases
Gene3D	2.40.20.10. 1 hit. 2.60.40.10. 1 hit.
InterPro	IPR020067. Frizzled_dom. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003598. Ig_sub2. IPR011009. Kinase-like_dom. IPR000001. Kringle. IPR013806. Kringle-like. IPR018056. Kringle_CS. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. IPR016247. Tyr_kinase_rcpt_ROR. [Graphical view]
Pfam	PF01392. Fz. 1 hit. PF07679. I-set. 1 hit. PF00051. Kringle. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PIRSF	PIRSF000624. TyrPK_TMrec_ROR. 1 hit.
PRINTS	PR00109. TYRKINASE.
SMART	SM00408. IGc2. 1 hit. SM00130. KR. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF57440. Kringle-like. 1 hit.
PROSITE	PS50038. FZ. 1 hit. PS50835. IG_LIKE. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ROR1. human.
GenomeRNAi	4919.
NextBio	18939.
SOURCE	Search...

Entry information

Entry name

ROR1_HUMAN

Accession

Primary (citable) accession number: Q01973
Secondary accession number(s): Q5VVX6, Q66K77, Q92776

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 28, 2006
Last modified:	May 1, 2013
This is version 135 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.