Reviewed,
UniProtKB/Swiss-Prot Q01970 (PLCB3_HUMAN)
Last modified
November 3, 2009.
Version 93.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3 EC=3.1.4.11 Alternative name(s): Phosphoinositide phospholipase C Phospholipase C-beta-3 Short name=PLC-beta-3 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1234 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. |
| Catalytic activity | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. |
| Cofactor | Calcium. |
| Subunit structure | Interacts with SHANK2 By similarity. Interacts with LPAR2. |
| Subcellular location | |
| Sequence similarities | Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid degradation |
| Cellular component | Membrane |
| Coding sequence diversity | Polymorphism |
| Ligand | Calcium |
| Molecular function | Hydrolase Transducer |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | intracellular signaling cascade Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytosol Inferred from Experiment. Source: Reactome extrinsic to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activityInferred from electronic annotation. Source: EC signal transducer activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1234 | 1234 | 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3 | PRO_0000088491 | |||||
Regions | |||||||||
| Domain | 318 – 468 | 151 | PI-PLC X-box | ||||||
| Domain | 590 – 706 | 117 | PI-PLC Y-box | ||||||
| Domain | 713 – 810 | 98 | C2 | ||||||
| Region | 1231 – 1234 | 4 | Interaction with SHANK2 By similarity | ||||||
Sites | |||||||||
| Active site | 332 | 1 | By similarity | ||||||
| Active site | 379 | 1 | By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 537 | 1 | Phosphoserine Ref.5 Ref.7 | ||||||
| Modified residue | 926 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 931 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 934 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 1122 | 1 | Phosphothreonine Ref.6 | ||||||
Natural variations | |||||||||
| Natural variant | 483 | 1 | R → H: dbSNP rs12146487. | VAR_029229 | |||||
Experimental info | |||||||||
| Sequence conflict | 845 | 1 | S → L in CAA78903. Ref.3 | ||||||
| Sequence conflict | 881 | 1 | G → E in CAA85776. Ref.2 | ||||||
| Sequence conflict | 1089 – 1119 | 31 | REKKE…HKKEA → SWPSWPRSVRSSGRGSPRRS AGACWARCRRG in CAA85776. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structural organization and expression of the human phosphatidylinositol-specific phospholipase C beta-3 gene." Mazuruk K., Schoen T.J., Chader G.J., Rodriguez I.R. Biochem. Biophys. Res. Commun. 212:190-195(1995) [PubMed: 7612006] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Genomic organization and complete cDNA sequence of the human phosphoinositide-specific phospholipase C beta 3 gene (PLCB3)." Lagercrantz J., Carson E., Phelan C., Grimmond S., Rosen A., Dare E., Nordenskjoeld M., Hayward N.K., Larsson C., Weber G. Genomics 26:467-472(1995) [PubMed: 7607669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Identification, purification and characterization of a novel phosphatidylinositol-specific phospholipase C, a third member of the beta subfamily." Carozzi A.J., Kriz R.W., Webster C., Parker P.J. Eur. J. Biochem. 210:521-529(1992) [PubMed: 1333955] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 184-1234. |
| [4] | "NHERF2 specifically interacts with LPA2 receptor and defines the specificity and efficiency of receptor-mediated phospholipase C-beta3 activation." Oh Y.-S., Jo N.W., Choi J.W., Kim H.S., Seo S.-W., Kang K.-O., Hwang J.-I., Heo K., Kim S.-H., Kim Y.-H., Kim I.-H., Kim J.H., Banno Y., Ryu S.H., Suh P.-G. Mol. Cell. Biol. 24:5069-5079(2004) [PubMed: 15143197] [Abstract] Cited for: INTERACTION WITH LPAR2. |
| [5] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1122, MASS SPECTROMETRY. Tissue: Epithelium. |
| [7] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537; SER-926; SER-931 AND SER-934, MASS SPECTROMETRY. |
| [8] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| U26425 Genomic DNA. Translation: AAA77683.1. Z37544 Z37574 Genomic DNA. Translation: CAA85776.1. Z16411 mRNA. Translation: CAA78903.1. | |
| IPI | IPI00010400. |
| PIR | I38994. S27002. S52099. |
| RefSeq | NP_000923.1. |
| UniGene | Hs.591953 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DJX based on UniProtKB P10688. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q01970. |
PTM databases | |
| PhosphoSite | Q01970. |
Proteomic databases | |
| PRIDE | Q01970. |
Genome annotation databases | |
| Ensembl | ENST00000279230; ENSP00000279230; ENSG00000149782; Homo sapiens. [Genome view] ENST00000325234; ENSP00000324660; ENSG00000149782; Homo sapiens. [Genome view] |
| GeneID | 5331. |
| KEGG | hsa:5331. |
| UCSC | uc001nzb.2. human. |
Organism-specific databases | |
| CTD | 5331. |
| GeneCards | GC11P063775. |
| H-InvDB | HIX0009758. |
| HGNC | HGNC:9056. PLCB3. |
| HPA | CAB009257. |
| MIM | 600230. gene. |
| PharmGKB | PA33386. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q01970. |
| HOVERGEN | Q01970. |
| OMA | KEMNERE. |
Enzyme and pathway databases | |
| BRENDA | 3.1.4.11. 247. |
| Pathway_Interaction_DB | lysophospholipid_pathway. LPA receptor mediated events. |
| Reactome | REACT_14797. Signaling by GPCR. REACT_15295. Opioid Signalling. REACT_15380. Diabetes pathways. REACT_19140. ADP signalling through P2Y purinoceptor 1. REACT_604. Hemostasis. |
Gene expression databases | |
| ArrayExpress | Q01970. |
| Bgee | Q01970. |
| CleanEx | HS_PLCB3. |
| Genevestigator | Q01970. |
| GermOnline | ENSG00000149782. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR011992. EF-Hand_type. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR000909. Phospholipase_C_Pinositol-sp_X. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR016280. PLC-beta. IPR014815. PLC-beta_C. IPR017946. PLC-like_Pdiesterase_TIM-brl. [Graphical view] |
| Gene3D | G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit. |
| Pfam | PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. PF08703. PLC-beta_C. 1 hit. [Graphical view] |
| PIRSF | PIRSF000956. PLC-beta. 1 hit. |
| PRINTS | PR00390. PHPHLIPASEC. |
| ProDom | PD001202. PI_PLC_Y. 2 hits. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view] |
| PROSITE | PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 20644. |
| SOURCE | Search... |
Entry information
| Entry name | PLCB3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q01970 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 11 Human chromosome 11: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


