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Protein

Flagellar biosynthesis protein FlhF

Gene

flhF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi182 – 1898GTPBy similarity
Nucleotide bindingi259 – 2635GTPBy similarity
Nucleotide bindingi317 – 3204GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Bacterial flagellum biogenesis, Bacterial flagellum protein export, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU16400-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar biosynthesis protein FlhF
Alternative name(s):
Flagella-associated GTP-binding protein
Gene namesi
Name:flhF
Ordered Locus Names:BSU16400
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Flagellar biosynthesis protein FlhFPRO_0000101220Add
BLAST

Proteomic databases

PaxDbiQ01960.

Interactioni

Protein-protein interaction databases

DIPiDIP-46493N.
STRINGi224308.Bsubs1_010100009046.

Structurei

Secondary structure

1
366
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi111 – 12111Combined sources
Helixi126 – 13813Combined sources
Beta strandi141 – 1444Combined sources
Turni148 – 1503Combined sources
Helixi151 – 16010Combined sources
Helixi166 – 1683Combined sources
Beta strandi175 – 1839Combined sources
Helixi188 – 20114Combined sources
Beta strandi207 – 2115Combined sources
Helixi219 – 2279Combined sources
Turni228 – 2314Combined sources
Beta strandi234 – 2363Combined sources
Helixi240 – 24910Combined sources
Helixi250 – 2523Combined sources
Beta strandi253 – 2597Combined sources
Helixi268 – 27710Combined sources
Beta strandi284 – 2918Combined sources
Helixi296 – 3027Combined sources
Turni303 – 3064Combined sources
Beta strandi307 – 3093Combined sources
Beta strandi313 – 3175Combined sources
Turni319 – 3213Combined sources
Helixi326 – 3338Combined sources
Beta strandi340 – 3423Combined sources
Turni348 – 3503Combined sources
Beta strandi351 – 3533Combined sources
Helixi357 – 3648Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PX0X-ray3.00A/B/C/D/E/F/G/H79-366[»]
2PX3X-ray3.20A79-366[»]
3SYNX-ray3.06A/B/C/D79-366[»]
ProteinModelPortaliQ01960.
SMRiQ01960. Positions 109-366.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01960.

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP-binding SRP family.Curated

Phylogenomic databases

eggNOGiENOG4105FZW. Bacteria.
COG1419. LUCA.
HOGENOMiHOG000254735.
InParanoidiQ01960.
KOiK02404.
OMAiWYEVDEY.
OrthoDBiEOG618QQ6.
PhylomeDBiQ01960.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR020006. FlhF.
IPR027417. P-loop_NTPase.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PANTHERiPTHR11564:SF3. PTHR11564:SF3. 1 hit.
PfamiPF00448. SRP54. 1 hit.
[Graphical view]
SMARTiSM00962. SRP54. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03499. FlhF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKKFTAAS MQEAALLIRK ELGNEAVILN SKKIKKRKWF GLVNKPAVEV
60 70 80 90 100
IAVLDQDFLE KKTPQKAAEP KQTLKTPVSS PKIEERTYPP QIPAQQELGD
110 120 130 140 150
FSAYQSVLPE PLRKAEKLLQ ETGIKESTKT NTLKKLLRFS VEAGGLTEEN
160 170 180 190 200
VVGKLQEILC DMLPSADKWQ EPIHSKYIVL FGSTGAGKTT TLAKLAAISM
210 220 230 240 250
LEKHKKIAFI TTDTYRIAAV EQLKTYAELL QAPLEVCYTK EEFQQAKELF
260 270 280 290 300
SEYDHVFVDT AGRNFKDPQY IDELKETIPF ESSIQSFLVL SATAKYEDMK
310 320 330 340 350
HIVKRFSSVP VNQYIFTKID ETTSLGSVFN ILAESKIGVG FMTNGQNVPE
360
DIQTVSPLGF VRMLCR
Length:366
Mass (Da):41,150
Last modified:June 1, 1994 - v1
Checksum:iE5A3577E49D16F1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66445 Genomic DNA. Translation: CAA47062.1.
AL009126 Genomic DNA. Translation: CAB13513.1.
PIRiS25277.
RefSeqiNP_389522.1. NC_000964.3.
WP_003231945.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13513; CAB13513; BSU16400.
GeneIDi940021.
KEGGibsu:BSU16400.
PATRICi18975087. VBIBacSub10457_1735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66445 Genomic DNA. Translation: CAA47062.1.
AL009126 Genomic DNA. Translation: CAB13513.1.
PIRiS25277.
RefSeqiNP_389522.1. NC_000964.3.
WP_003231945.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PX0X-ray3.00A/B/C/D/E/F/G/H79-366[»]
2PX3X-ray3.20A79-366[»]
3SYNX-ray3.06A/B/C/D79-366[»]
ProteinModelPortaliQ01960.
SMRiQ01960. Positions 109-366.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46493N.
STRINGi224308.Bsubs1_010100009046.

Proteomic databases

PaxDbiQ01960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13513; CAB13513; BSU16400.
GeneIDi940021.
KEGGibsu:BSU16400.
PATRICi18975087. VBIBacSub10457_1735.

Phylogenomic databases

eggNOGiENOG4105FZW. Bacteria.
COG1419. LUCA.
HOGENOMiHOG000254735.
InParanoidiQ01960.
KOiK02404.
OMAiWYEVDEY.
OrthoDBiEOG618QQ6.
PhylomeDBiQ01960.

Enzyme and pathway databases

BioCyciBSUB:BSU16400-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ01960.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR020006. FlhF.
IPR027417. P-loop_NTPase.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PANTHERiPTHR11564:SF3. PTHR11564:SF3. 1 hit.
PfamiPF00448. SRP54. 1 hit.
[Graphical view]
SMARTiSM00962. SRP54. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03499. FlhF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "flhF, a Bacillus subtilis flagellar gene that encodes a putative GTP-binding protein."
    Carpenter P.B., Hanlon D.W., Ordal G.W.
    Mol. Microbiol. 6:2705-2713(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiFLHF_BACSU
AccessioniPrimary (citable) accession number: Q01960
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.