Q01956 (KCNC3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily C member 3

Alternative name(s):
KSHIIID
Voltage-gated potassium channel subunit Kv3.3

Gene names

Name:

Kcnc3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	889 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Subunit structure	Heterotetramer of potassium channel proteins By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
Sequence similarities	Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.3/KCNC3 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein homooligomerization Inferred from electronic annotation. Source: InterPro
Cellular_component	voltage-gated potassium channel complex Inferred from electronic annotation. Source: InterPro
Molecular_function	delayed rectifier potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform KSHIIID.1 (identifier: Q01956-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform KSHIIID.2 (identifier: Q01956-2)

The sequence of this isoform differs from the canonical sequence as follows:
     662-679: NDLGVLEEGDPRPNGDPA → EAGARTGGVGRPGGWGSG
     680-889: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 889	889	Potassium voltage-gated channel subfamily C member 3		PRO_0000054057
Regions
	Topological domain	1 – 291	291	Cytoplasmic Potential
	Transmembrane	292 – 310	19	Helical; Name=Segment S1; Potential
	Transmembrane	352 – 371	20	Helical; Name=Segment S2; Potential
	Topological domain	372 – 380	9	Cytoplasmic Potential
	Transmembrane	381 – 399	19	Helical; Name=Segment S3; Potential
	Transmembrane	413 – 435	23	Helical; Voltage-sensor; Name=Segment S4; Potential
	Topological domain	436 – 448	13	Cytoplasmic Potential
	Transmembrane	449 – 470	22	Helical; Name=Segment S5; Potential
	Transmembrane	519 – 540	22	Helical; Name=Segment S6; Potential
	Topological domain	541 – 889	349	Cytoplasmic Potential
	Motif	504 – 509	6	Selectivity filter By similarity
Amino acid modifications
	Glycosylation	321	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	662 – 679	18	NDLGV…NGDPA → EAGARTGGVGRPGGWGSG in isoform KSHIIID.2.		VSP_001022
	Alternative sequence	680 – 889	210	Missing in isoform KSHIIID.2.		VSP_001023

Sequences

	Sequence		Length	Mass (Da)
	Isoform KSHIIID.1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 51424C047B4FC367	FASTA	889	94,393
	10 20 30 40 50 60 MLSSVCVWSF SGRQGTRKQH SQPAPTPQPP ESSPPPLLPP PQQQCAQPGT AASPAGAPLS 70 80 90 100 110 120 CGPGGRRAEP CSGLPAVAMG RHGGGGGDSG KIVINVGGVR HETYRSTLRT LPGTRLAGLT 130 140 150 160 170 180 EPEAAARFDY DPGTDEFFFD RHPGVFAYVL NYYRTGKLHC PADVCGPLFE EELGFWGIDE 190 200 210 220 230 240 TDVEACCWMT YRQHRDAEEA LDSFEAPDSS GNANANAGGA HDAGLDDEAG AGGGGLDGAG 250 260 270 280 290 300 GELKRLCFQD AGGGAGGPAG GPGGAGGTWW RRWQPRVWAL FEDPYSSRAA RYVAFASLFF 310 320 330 340 350 360 ILISITTFCL ETHEGFIHIS NKTVTQASPI PGAPPENITN VEVETEPFLT YVEGVCVVWF 370 380 390 400 410 420 TFEFLMRVTF CPDKVEFLKS SLNIIDCVAI LPFYLEVGLS GLSSKAAKDV LGFLRVVRFV 430 440 450 460 470 480 RILRIFKLTR HFVGLRVLGH TLRASTNEFL LLIIFLALGV LIFATMIYYA ERIGADPDDI 490 500 510 520 530 540 LGSNHTYFKN IPIGFWWAVV TMTTLGYGDM YPKTWSGMLV GALCALAGVL TIAMPVPVIV 550 560 570 580 590 600 NNFGMYYSLA MAKQKLPKKK NKHIPRPPQP GSPNYCKPDP PPPPPPHPHH GSGGISPPPP 610 620 630 640 650 660 ITPPSMGVTV AGAYPPGPHT HPGLLRGGAG GLGIMGLPPL PAPGEPCPLA QEEVIETNRA 670 680 690 700 710 720 GNDLGVLEEG DPRPNGDPAA AALAHEDCPA IDQPAMSPED KSPITPGSRG RYSRDRACFL 730 740 750 760 770 780 VTDYAPSPDG SIRKATGAPP LPPHAGVSQA PPASCPTSTP TQQPGYPPSG RAPSPPQATP 790 800 810 820 830 840 EAPAIFDVWL PPFHRSHQPP GKHQRGGRHP GVSPSPQQRA CVGEPPSASH PQSLTLCISV 850 860 870 880 PSSCHRLRPR ETLGFPLSLP PRLATGNGGR ECPRDPGLPF PSRHSSPAV
	Isoform KSHIIID.2 [UniParc]. Checksum: 077D2D9EE373D0F8	FASTA	679	72,170
	10 20 30 40 50 60 MLSSVCVWSF SGRQGTRKQH SQPAPTPQPP ESSPPPLLPP PQQQCAQPGT AASPAGAPLS 70 80 90 100 110 120 CGPGGRRAEP CSGLPAVAMG RHGGGGGDSG KIVINVGGVR HETYRSTLRT LPGTRLAGLT 130 140 150 160 170 180 EPEAAARFDY DPGTDEFFFD RHPGVFAYVL NYYRTGKLHC PADVCGPLFE EELGFWGIDE 190 200 210 220 230 240 TDVEACCWMT YRQHRDAEEA LDSFEAPDSS GNANANAGGA HDAGLDDEAG AGGGGLDGAG 250 260 270 280 290 300 GELKRLCFQD AGGGAGGPAG GPGGAGGTWW RRWQPRVWAL FEDPYSSRAA RYVAFASLFF 310 320 330 340 350 360 ILISITTFCL ETHEGFIHIS NKTVTQASPI PGAPPENITN VEVETEPFLT YVEGVCVVWF 370 380 390 400 410 420 TFEFLMRVTF CPDKVEFLKS SLNIIDCVAI LPFYLEVGLS GLSSKAAKDV LGFLRVVRFV 430 440 450 460 470 480 RILRIFKLTR HFVGLRVLGH TLRASTNEFL LLIIFLALGV LIFATMIYYA ERIGADPDDI 490 500 510 520 530 540 LGSNHTYFKN IPIGFWWAVV TMTTLGYGDM YPKTWSGMLV GALCALAGVL TIAMPVPVIV 550 560 570 580 590 600 NNFGMYYSLA MAKQKLPKKK NKHIPRPPQP GSPNYCKPDP PPPPPPHPHH GSGGISPPPP 610 620 630 640 650 660 ITPPSMGVTV AGAYPPGPHT HPGLLRGGAG GLGIMGLPPL PAPGEPCPLA QEEVIETNRA 670 GEAGARTGGV GRPGGWGSG

References

[1]	"Cloning of ShIII (Shaw-like) cDNAs encoding a novel high-voltage-activating, TEA-sensitive, type-A K+ channel." de Miera E.V.-S., Moreno H., Fruhling D., Kentros C., Rudy B. Proc. R. Soc. B 248:9-18(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS KSHIIID.1 AND KSHIIID.2). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84210 mRNA. Translation: AAA73182.1. M84211 mRNA. Translation: AAA41470.1.
IPI	IPI00197989. IPI00197991.
UniGene	Rn.9885.
3D structure databases
ProteinModelPortal	Q01956.
SMR	Q01956. Positions 91-195.
ModBase	Search...
Proteomic databases
PaxDb	Q01956.
PRIDE	Q01956.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:621358. rat.
Organism-specific databases
RGD	621358. Kcnc3.
Phylogenomic databases
eggNOG	NOG280627.
HOGENOM	HOG000231012.
HOVERGEN	HBG105862.
InParanoid	Q01956.
Gene expression databases
ArrayExpress	Q01956.
Genevestigator	Q01956.
GermOnline	ENSRNOG00000019959. Rattus norvegicus.
Family and domain databases
Gene3D	3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR005821. Ion_trans_dom. IPR003091. K_chnl. IPR003968. K_chnl_volt-dep_Kv. IPR003974. K_chnl_volt-dep_Kv3. IPR005404. K_chnl_volt-dep_Kv3.3. IPR003131. T1-type_BTB. [Graphical view]
PANTHER	PTHR11537. PTHR11537. 1 hit.
Pfam	PF00520. Ion_trans. 1 hit. PF02214. K_tetra. 1 hit. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01582. KV33CHANNEL. PR01491. KVCHANNEL. PR01498. SHAWCHANNEL.
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KCNC3_RAT

Accession

Primary (citable) accession number: Q01956

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program