Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium voltage-gated channel subfamily C member 3

Gene

Kcnc3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient. The channel displays rapid activation and inactivation kinetics (PubMed:1381835). It plays a role in the regulation of the frequency, shape and duration of action potentials in Purkinje cells. Required for normal survival of cerebellar neurons, probably via its role in regulating the duration and frequency of action potentials that in turn regulate the activity of voltage-gated Ca2+ channels and cellular Ca2+ homeostasis. Required for normal motor function (By similarity). Plays a role in the reorganization of the cortical actin cytoskeleton and the formation of actin veil structures in neuronal growth cones via its interaction with HAX1 and the Arp2/3 complex (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily C member 3
Alternative name(s):
KSHIIID1 Publication
Voltage-gated potassium channel subunit Kv3.31 Publication
Gene namesi
Name:Kcnc3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621358. Kcnc3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 291CytoplasmicSequence analysisAdd BLAST291
Transmembranei292 – 310Helical; Name=Segment S1Sequence analysisAdd BLAST19
Transmembranei352 – 371Helical; Name=Segment S2Sequence analysisAdd BLAST20
Topological domaini372 – 380CytoplasmicSequence analysis9
Transmembranei381 – 399Helical; Name=Segment S3Sequence analysisAdd BLAST19
Transmembranei413 – 435Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST23
Topological domaini436 – 448CytoplasmicSequence analysisAdd BLAST13
Transmembranei449 – 470Helical; Name=Segment S5Sequence analysisAdd BLAST22
Transmembranei519 – 540Helical; Name=Segment S6Sequence analysisAdd BLAST22
Topological domaini541 – 889CytoplasmicSequence analysisAdd BLAST349

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Synapse

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi550.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000540571 – 889Potassium voltage-gated channel subfamily C member 3Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Modified residuei626Omega-N-methylarginineBy similarity1
Modified residuei697PhosphoserineBy similarity1
Modified residuei702PhosphoserineBy similarity1
Modified residuei759PhosphothreonineBy similarity1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ01956.
PRIDEiQ01956.

Expressioni

Tissue specificityi

Detected on Purkinje cells in the cerebellum molecular layer (at protein level).1 Publication

Interactioni

Subunit structurei

Homotetramer. Heterotetramer with KCNC1. Interacts (via C-terminus) with HAX1. Identified in a complex with ACTR3, a subunit of the Arp2/3 complex; this interaction is indirect and depends on the presence of HAX1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027062.

Structurei

3D structure databases

ProteinModelPortaliQ01956.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 80Important for normal N-type inactivationBy similarityAdd BLAST80

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi504 – 509Selectivity filterBy similarity6

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.Curated
The cytoplasmic N-terminus mediates N-type inactivation.By similarity
The C-terminal cytoplasmic tail contributes to the regulation of channel inactivation and to the interaction with HAX1 and the Arp2/3 complex.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
HOGENOMiHOG000231012.
HOVERGENiHBG105862.
InParanoidiQ01956.
PhylomeDBiQ01956.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003974. K_chnl_volt-dep_Kv3.
IPR005404. K_chnl_volt-dep_Kv3.3.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 3 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01582. KV33CHANNEL.
PR01491. KVCHANNEL.
PR01498. SHAWCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform KSHIIID.1 (identifier: Q01956-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSSVCVWSF SGRQGTRKQH SQPAPTPQPP ESSPPPLLPP PQQQCAQPGT
60 70 80 90 100
AASPAGAPLS CGPGGRRAEP CSGLPAVAMG RHGGGGGDSG KIVINVGGVR
110 120 130 140 150
HETYRSTLRT LPGTRLAGLT EPEAAARFDY DPGTDEFFFD RHPGVFAYVL
160 170 180 190 200
NYYRTGKLHC PADVCGPLFE EELGFWGIDE TDVEACCWMT YRQHRDAEEA
210 220 230 240 250
LDSFEAPDSS GNANANAGGA HDAGLDDEAG AGGGGLDGAG GELKRLCFQD
260 270 280 290 300
AGGGAGGPAG GPGGAGGTWW RRWQPRVWAL FEDPYSSRAA RYVAFASLFF
310 320 330 340 350
ILISITTFCL ETHEGFIHIS NKTVTQASPI PGAPPENITN VEVETEPFLT
360 370 380 390 400
YVEGVCVVWF TFEFLMRVTF CPDKVEFLKS SLNIIDCVAI LPFYLEVGLS
410 420 430 440 450
GLSSKAAKDV LGFLRVVRFV RILRIFKLTR HFVGLRVLGH TLRASTNEFL
460 470 480 490 500
LLIIFLALGV LIFATMIYYA ERIGADPDDI LGSNHTYFKN IPIGFWWAVV
510 520 530 540 550
TMTTLGYGDM YPKTWSGMLV GALCALAGVL TIAMPVPVIV NNFGMYYSLA
560 570 580 590 600
MAKQKLPKKK NKHIPRPPQP GSPNYCKPDP PPPPPPHPHH GSGGISPPPP
610 620 630 640 650
ITPPSMGVTV AGAYPPGPHT HPGLLRGGAG GLGIMGLPPL PAPGEPCPLA
660 670 680 690 700
QEEVIETNRA GNDLGVLEEG DPRPNGDPAA AALAHEDCPA IDQPAMSPED
710 720 730 740 750
KSPITPGSRG RYSRDRACFL VTDYAPSPDG SIRKATGAPP LPPHAGVSQA
760 770 780 790 800
PPASCPTSTP TQQPGYPPSG RAPSPPQATP EAPAIFDVWL PPFHRSHQPP
810 820 830 840 850
GKHQRGGRHP GVSPSPQQRA CVGEPPSASH PQSLTLCISV PSSCHRLRPR
860 870 880
ETLGFPLSLP PRLATGNGGR ECPRDPGLPF PSRHSSPAV
Length:889
Mass (Da):94,393
Last modified:November 1, 1996 - v1
Checksum:i51424C047B4FC367
GO
Isoform KSHIIID.2 (identifier: Q01956-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     662-679: NDLGVLEEGDPRPNGDPA → EAGARTGGVGRPGGWGSG
     680-889: Missing.

Show »
Length:679
Mass (Da):72,170
Checksum:i077D2D9EE373D0F8
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001022662 – 679NDLGV…NGDPA → EAGARTGGVGRPGGWGSG in isoform KSHIIID.2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_001023680 – 889Missing in isoform KSHIIID.2. 1 PublicationAdd BLAST210

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84210 mRNA. Translation: AAA73182.1.
M84211 mRNA. Translation: AAA41470.1.
UniGeneiRn.9885.

Genome annotation databases

UCSCiRGD:621358. rat. [Q01956-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84210 mRNA. Translation: AAA73182.1.
M84211 mRNA. Translation: AAA41470.1.
UniGeneiRn.9885.

3D structure databases

ProteinModelPortaliQ01956.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027062.

Chemistry databases

GuidetoPHARMACOLOGYi550.

Proteomic databases

PaxDbiQ01956.
PRIDEiQ01956.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621358. rat. [Q01956-1]

Organism-specific databases

RGDi621358. Kcnc3.

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
HOGENOMiHOG000231012.
HOVERGENiHBG105862.
InParanoidiQ01956.
PhylomeDBiQ01956.

Miscellaneous databases

PROiQ01956.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003974. K_chnl_volt-dep_Kv3.
IPR005404. K_chnl_volt-dep_Kv3.3.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 3 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01582. KV33CHANNEL.
PR01491. KVCHANNEL.
PR01498. SHAWCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNC3_RAT
AccessioniPrimary (citable) accession number: Q01956
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.