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Q01954

- BNC1_HUMAN

UniProt

Q01954 - BNC1_HUMAN

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Protein

Zinc finger protein basonuclin-1

Gene

BNC1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri357 – 38024C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri385 – 41430C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri720 – 74324C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri748 – 77528C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri928 – 95124C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri956 – 98328C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. chromosome organization Source: Ensembl
  2. epidermis development Source: ProtInc
  3. positive regulation of cell proliferation Source: ProtInc
  4. positive regulation of epithelial cell proliferation Source: Ensembl
  5. regulation of transcription from RNA polymerase II promoter Source: Ensembl
  6. regulation of transcription from RNA polymerase I promoter Source: Ensembl
  7. transcription, DNA-templated Source: UniProtKB-KW
  8. wound healing Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein basonuclin-1
Gene namesi
Name:BNC1
Synonyms:BNC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:1081. BNC1.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity
Note: Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi537 – 5371S → A: No effect on phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-541. 1 Publication
Mutagenesisi537 – 5371S → D: Reduces phosphorylation and induces partial relocation into the cytoplasm. 1 Publication
Mutagenesisi540 – 5401S → D: No effect on phosphorylation, no effect on subcellular location. 1 Publication
Mutagenesisi541 – 5411S → A: Strongly reduces phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-537. 1 Publication
Mutagenesisi541 – 5411S → D: Strongly reduces phosphorylation and induces partial relocation into the cytoplasm. 1 Publication

Organism-specific databases

PharmGKBiPA25391.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 994994Zinc finger protein basonuclin-1PRO_0000046932Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei537 – 5371Phosphoserine1 Publication
Modified residuei541 – 5411Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01954.
PaxDbiQ01954.
PRIDEiQ01954.

PTM databases

PhosphoSiteiQ01954.

Expressioni

Tissue specificityi

Expressed in epidermis, testis and foreskin, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart.1 Publication

Gene expression databases

BgeeiQ01954.
CleanExiHS_BNC1.
ExpressionAtlasiQ01954. baseline and differential.
GenevestigatoriQ01954.

Interactioni

Protein-protein interaction databases

BioGridi107115. 1 interaction.
STRINGi9606.ENSP00000307041.

Structurei

3D structure databases

ProteinModelPortaliQ01954.
SMRiQ01954. Positions 351-419, 716-782, 854-881, 918-959.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni240 – 24910Hydrophobic

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi533 – 5397Nuclear localization signal1 Publication

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi859 – 87618Ser-richAdd
BLAST

Sequence similaritiesi

Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri357 – 38024C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri385 – 41430C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri720 – 74324C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri748 – 77528C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri928 – 95124C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri956 – 98328C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG82846.
GeneTreeiENSGT00390000005844.
HOGENOMiHOG000049098.
HOVERGENiHBG050706.
InParanoidiQ01954.
OMAiTLNCSCQ.
OrthoDBiEOG7SV0TM.
PhylomeDBiQ01954.
TreeFamiTF350399.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01954-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRRPPSRGG RGAARARETR RQPRHRSGRR MAEAISCTLN CSCQSFKPGK
60 70 80 90 100
INHRQCDQCK HGWVAHALSK LRIPPMYPTS QVEIVQSNVV FDISSLMLYG
110 120 130 140 150
TQAIPVRLKI LLDRLFSVLK QDEVLQILHA LDWTLQDYIR GYVLQDASGK
160 170 180 190 200
VLDHWSIMTS EEEVATLQQF LRFGETKSIV ELMAIQEKEE QSIIIPPSTA
210 220 230 240 250
NVDIRAFIES CSHRSSSLPT PVDKGNPSSI HPFENLISNM TFMLPFQFFN
260 270 280 290 300
PLPPALIGSL PEQYMLEQGH DQSQDPKQEV HGPFPDSSFL TSSSTPFQVE
310 320 330 340 350
KDQCLNCPDA ITKKEDSTHL SDSSSYNIVT KFERTQLSPE AKVKPERNSL
360 370 380 390 400
GTKKGRVFCT ACEKTFYDKG TLKIHYNAVH LKIKHKCTIE GCNMVFSSLR
410 420 430 440 450
SRNRHSANPN PRLHMPMNRN NRDKDLRNSL NLASSENYKC PGFTVTSPDC
460 470 480 490 500
RPPPSYPGSG EDSKGQPAFP NIGQNGVLFP NLKTVQPVLP FYRSPATPAE
510 520 530 540 550
VANTPGILPS LPLLSSSIPE QLISNEMPFD ALPKKKSRKS SMPIKIEKEA
560 570 580 590 600
VEIANEKRHN LSSDEDMPLQ VVSEDEQEAC SPQSHRVSEE QHVQSGGLGK
610 620 630 640 650
PFPEGERPCH RESVIESSGA ISQTPEQATH NSERETEQTP ALIMVPREVE
660 670 680 690 700
DGGHEHYFTP GMEPQVPFSD YMELQQRLLA GGLFSALSNR GMAFPCLEDS
710 720 730 740 750
KELEHVGQHA LARQIEENRF QCDICKKTFK NACSVKIHHK NMHVKEMHTC
760 770 780 790 800
TVEGCNATFP SRRSRDRHSS NLNLHQKALS QEALESSEDH FRAAYLLKDV
810 820 830 840 850
AKEAYQDVAF TQQASQTSVI FKGTSRMGSL VYPITQVHSA SLESYNSGPL
860 870 880 890 900
SEGTILDLST TSSMKSESSS HSSWDSDGVS EEGTVLMEDS DGNCEGSSLV
910 920 930 940 950
PGEDEYPICV LMEKADQSLA SLPSGLPITC HLCQKTYSNK GTFRAHYKTV
960 970 980 990
HLRQLHKCKV PGCNTMFSSV RSRNRHSQNP NLHKSLASSP SHLQ
Length:994
Mass (Da):110,972
Last modified:December 1, 2000 - v2
Checksum:i7DF13EE782E20ED5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 149PSRGGRGAA → EPGRTRGG in AAA35584. (PubMed:1332044)Curated
Sequence conflicti156 – 1561S → C in AAA35584. (PubMed:1332044)Curated
Sequence conflicti284 – 2841F → N in AAA35584. (PubMed:1332044)Curated
Sequence conflicti401 – 4011S → T in AAA35584. (PubMed:1332044)Curated
Sequence conflicti638 – 6381Q → H in AAA35584. (PubMed:1332044)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L03427 mRNA. Translation: AAA35584.1.
U59694, U59692, U59693 Genomic DNA. Translation: AAB53028.1.
CCDSiCCDS10324.1.
PIRiA46415.
RefSeqiNP_001708.3. NM_001717.3.
UniGeneiHs.459153.

Genome annotation databases

EnsembliENST00000345382; ENSP00000307041; ENSG00000169594.
GeneIDi646.
KEGGihsa:646.
UCSCiuc002bjt.1. human.

Polymorphism databases

DMDMi12644377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L03427 mRNA. Translation: AAA35584.1 .
U59694 , U59692 , U59693 Genomic DNA. Translation: AAB53028.1 .
CCDSi CCDS10324.1.
PIRi A46415.
RefSeqi NP_001708.3. NM_001717.3.
UniGenei Hs.459153.

3D structure databases

ProteinModelPortali Q01954.
SMRi Q01954. Positions 351-419, 716-782, 854-881, 918-959.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107115. 1 interaction.
STRINGi 9606.ENSP00000307041.

PTM databases

PhosphoSitei Q01954.

Polymorphism databases

DMDMi 12644377.

Proteomic databases

MaxQBi Q01954.
PaxDbi Q01954.
PRIDEi Q01954.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000345382 ; ENSP00000307041 ; ENSG00000169594 .
GeneIDi 646.
KEGGi hsa:646.
UCSCi uc002bjt.1. human.

Organism-specific databases

CTDi 646.
GeneCardsi GC15M083924.
HGNCi HGNC:1081. BNC1.
MIMi 601930. gene.
neXtProti NX_Q01954.
PharmGKBi PA25391.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG82846.
GeneTreei ENSGT00390000005844.
HOGENOMi HOG000049098.
HOVERGENi HBG050706.
InParanoidi Q01954.
OMAi TLNCSCQ.
OrthoDBi EOG7SV0TM.
PhylomeDBi Q01954.
TreeFami TF350399.

Miscellaneous databases

GeneWikii BNC1.
GenomeRNAii 646.
NextBioi 2622.
PROi Q01954.
SOURCEi Search...

Gene expression databases

Bgeei Q01954.
CleanExi HS_BNC1.
ExpressionAtlasi Q01954. baseline and differential.
Genevestigatori Q01954.

Family and domain databases

InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF00096. zf-C2H2. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 6 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Basonuclin: a keratinocyte protein with multiple paired zinc fingers."
    Tseng H., Green H.
    Proc. Natl. Acad. Sci. U.S.A. 89:10311-10315(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Keratinocyte.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Nuclear localization of basonuclin in human keratinocytes and the role of phosphorylation."
    Iuchi S., Green H.
    Proc. Natl. Acad. Sci. U.S.A. 94:7948-7953(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, PHOSPHORYLATION AT SER-537 AND SER-541, MUTAGENESIS OF SER-537; SER-540 AND SER-541.
  4. "Translocation of the zinc finger protein basonuclin from the mouse germ cell nucleus to the midpiece of the spermatozoon during spermiogenesis."
    Mahoney M.G., Tang W., Xiang M.M., Moss S.B., Gerton G.L., Stanley J.R., Tseng H.
    Biol. Reprod. 59:388-394(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiBNC1_HUMAN
AccessioniPrimary (citable) accession number: Q01954
Secondary accession number(s): Q15840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3