Q01954 (BNC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein basonuclin-1

Gene names

Name:	BNC1
Synonyms:	BNC

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	994 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids By similarity. Ref.3
Tissue specificity	Expressed in epidermis, testis and foreskin, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart. Ref.4
Post-translational modification	Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.
Sequence similarities	Contains 6 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromosome organization Inferred from electronic annotation. Source: Compara epidermis development Traceable author statement Ref.1. Source: ProtInc positive regulation of cell proliferation Traceable author statement PubMed 8034748. Source: ProtInc positive regulation of epithelial cell proliferation Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase I promoter Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Traceable author statement PubMed 8034748. Source: ProtInc
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Traceable author statement Ref.2. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 994	994	Zinc finger protein basonuclin-1		PRO_0000046932
Regions
	Zinc finger	357 – 380	24	C2H2-type 1
	Zinc finger	385 – 414	30	C2H2-type 2
	Zinc finger	720 – 743	24	C2H2-type 3
	Zinc finger	748 – 775	28	C2H2-type 4
	Zinc finger	928 – 951	24	C2H2-type 5
	Zinc finger	956 – 983	28	C2H2-type 6
	Region	240 – 249	10	Hydrophobic
	Motif	533 – 539	7	Nuclear localization signal Ref.3
	Compositional bias	859 – 876	18	Ser-rich
Amino acid modifications
	Modified residue	537	1	Phosphoserine Ref.3
	Modified residue	541	1	Phosphoserine Ref.3
Experimental info
	Mutagenesis	537	1	S → A: No effect on phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-541. Ref.3
	Mutagenesis	537	1	S → D: Reduces phosphorylation and induces partial relocation into the cytoplasm. Ref.3
	Mutagenesis	540	1	S → D: No effect on phosphorylation, no effect on subcellular location. Ref.3
	Mutagenesis	541	1	S → A: Strongly reduces phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-537. Ref.3
	Mutagenesis	541	1	S → D: Strongly reduces phosphorylation and induces partial relocation into the cytoplasm. Ref.3
	Sequence conflict	6 – 14	9	PSRGGRGAA → EPGRTRGG in AAA35584. Ref.1
	Sequence conflict	156	1	S → C in AAA35584. Ref.1
	Sequence conflict	284	1	F → N in AAA35584. Ref.1
	Sequence conflict	401	1	S → T in AAA35584. Ref.1
	Sequence conflict	638	1	Q → H in AAA35584. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01954 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 7DF13EE782E20ED5	FASTA	994	110,972
	10 20 30 40 50 60 MRRRPPSRGG RGAARARETR RQPRHRSGRR MAEAISCTLN CSCQSFKPGK INHRQCDQCK 70 80 90 100 110 120 HGWVAHALSK LRIPPMYPTS QVEIVQSNVV FDISSLMLYG TQAIPVRLKI LLDRLFSVLK 130 140 150 160 170 180 QDEVLQILHA LDWTLQDYIR GYVLQDASGK VLDHWSIMTS EEEVATLQQF LRFGETKSIV 190 200 210 220 230 240 ELMAIQEKEE QSIIIPPSTA NVDIRAFIES CSHRSSSLPT PVDKGNPSSI HPFENLISNM 250 260 270 280 290 300 TFMLPFQFFN PLPPALIGSL PEQYMLEQGH DQSQDPKQEV HGPFPDSSFL TSSSTPFQVE 310 320 330 340 350 360 KDQCLNCPDA ITKKEDSTHL SDSSSYNIVT KFERTQLSPE AKVKPERNSL GTKKGRVFCT 370 380 390 400 410 420 ACEKTFYDKG TLKIHYNAVH LKIKHKCTIE GCNMVFSSLR SRNRHSANPN PRLHMPMNRN 430 440 450 460 470 480 NRDKDLRNSL NLASSENYKC PGFTVTSPDC RPPPSYPGSG EDSKGQPAFP NIGQNGVLFP 490 500 510 520 530 540 NLKTVQPVLP FYRSPATPAE VANTPGILPS LPLLSSSIPE QLISNEMPFD ALPKKKSRKS 550 560 570 580 590 600 SMPIKIEKEA VEIANEKRHN LSSDEDMPLQ VVSEDEQEAC SPQSHRVSEE QHVQSGGLGK 610 620 630 640 650 660 PFPEGERPCH RESVIESSGA ISQTPEQATH NSERETEQTP ALIMVPREVE DGGHEHYFTP 670 680 690 700 710 720 GMEPQVPFSD YMELQQRLLA GGLFSALSNR GMAFPCLEDS KELEHVGQHA LARQIEENRF 730 740 750 760 770 780 QCDICKKTFK NACSVKIHHK NMHVKEMHTC TVEGCNATFP SRRSRDRHSS NLNLHQKALS 790 800 810 820 830 840 QEALESSEDH FRAAYLLKDV AKEAYQDVAF TQQASQTSVI FKGTSRMGSL VYPITQVHSA 850 860 870 880 890 900 SLESYNSGPL SEGTILDLST TSSMKSESSS HSSWDSDGVS EEGTVLMEDS DGNCEGSSLV 910 920 930 940 950 960 PGEDEYPICV LMEKADQSLA SLPSGLPITC HLCQKTYSNK GTFRAHYKTV HLRQLHKCKV 970 980 990 PGCNTMFSSV RSRNRHSQNP NLHKSLASSP SHLQ

References

[1]	"Basonuclin: a keratinocyte protein with multiple paired zinc fingers." Tseng H., Green H. Proc. Natl. Acad. Sci. U.S.A. 89:10311-10315(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Keratinocyte.
[2]	"The human basonuclin gene." Teumer J., Tseng H., Green H. Gene 188:1-7(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nuclear localization of basonuclin in human keratinocytes and the role of phosphorylation." Iuchi S., Green H. Proc. Natl. Acad. Sci. U.S.A. 94:7948-7953(1997) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, PHOSPHORYLATION AT SER-537 AND SER-541, MUTAGENESIS OF SER-537; SER-540 AND SER-541.
[4]	"Translocation of the zinc finger protein basonuclin from the mouse germ cell nucleus to the midpiece of the spermatozoon during spermiogenesis." Mahoney M.G., Tang W., Xiang M.M., Moss S.B., Gerton G.L., Stanley J.R., Tseng H. Biol. Reprod. 59:388-394(1998) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03427 mRNA. Translation: AAA35584.1. U59694, U59692, U59693 Genomic DNA. Translation: AAB53028.1.
IPI	IPI00304972.
PIR	A46415.
RefSeq	NP_001708.3. NM_001717.3.
UniGene	Hs.459153.
3D structure databases
ProteinModelPortal	Q01954.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000307041.
PTM databases
PhosphoSite	Q01954.
Polymorphism databases
DMDM	12644377.
Proteomic databases
PaxDb	Q01954.
PRIDE	Q01954.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000345382; ENSP00000307041; ENSG00000169594.
GeneID	646.
KEGG	hsa:646.
UCSC	uc002bjt.1. human.
Organism-specific databases
CTD	646.
GeneCards	GC15M083924.
HGNC	HGNC:1081. BNC1.
MIM	601930. gene.
neXtProt	NX_Q01954.
PharmGKB	PA25391.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG82846.
HOGENOM	HOG000049098.
HOVERGEN	HBG050706.
InParanoid	Q01954.
OMA	MEKADQS.
OrthoDB	EOG4255S9.
PhylomeDB	Q01954.
Gene expression databases
ArrayExpress	Q01954.
Bgee	Q01954.
CleanEx	HS_BNC1.
Genevestigator	Q01954.
GermOnline	ENSG00000169594. Homo sapiens.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 6 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	646.
NextBio	2622.
SOURCE	Search...

Entry information

Entry name

BNC1_HUMAN

Accession

Primary (citable) accession number: Q01954
Secondary accession number(s): Q15840

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.