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Protein

Zinc finger protein basonuclin-1

Gene

BNC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator (By similarity). Likely specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation (PubMed:8034748). Required for the maintenance of spermatogenesis (By similarity). May also play a role in the differentiation of oocytes and the early development of embryos (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri357 – 380C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri385 – 414C2H2-type 2PROSITE-ProRule annotationAdd BLAST30
Zinc fingeri720 – 743C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri748 – 775C2H2-type 4PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri928 – 951C2H2-type 5PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri956 – 983C2H2-type 6PROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • epidermis development Source: ProtInc
  • positive regulation of cell proliferation Source: ProtInc
  • spermatogenesis Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169594-MONOMER.
SIGNORiQ01954.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein basonuclin-1
Gene namesi
Name:BNC1
Synonyms:BNC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:1081. BNC1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi537S → A: No effect on phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-541. 1 Publication1
Mutagenesisi537S → D: Reduces phosphorylation and induces partial relocation into the cytoplasm. 1 Publication1
Mutagenesisi540S → D: No effect on phosphorylation, no effect on subcellular location. 1 Publication1
Mutagenesisi541S → A: Strongly reduces phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-537. 1 Publication1
Mutagenesisi541S → D: Strongly reduces phosphorylation and induces partial relocation into the cytoplasm. 1 Publication1

Organism-specific databases

DisGeNETi646.
OpenTargetsiENSG00000169594.
PharmGKBiPA25391.

Polymorphism and mutation databases

BioMutaiBNC1.
DMDMi12644377.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000469321 – 994Zinc finger protein basonuclin-1Add BLAST994

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei537Phosphoserine1 Publication1
Modified residuei541Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ01954.
MaxQBiQ01954.
PaxDbiQ01954.
PeptideAtlasiQ01954.
PRIDEiQ01954.

PTM databases

iPTMnetiQ01954.
PhosphoSitePlusiQ01954.

Expressioni

Tissue specificityi

In epidermis, primarily detected in cells of the basal or immediately suprabasal layers (at protein level) (PubMed:16891417). In hair follicles, mainly expressed in the outer root sheath (at protein level) (PubMed:8034748). Expressed in epidermis, testis and foreskin, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart (PubMed:9687312).3 Publications

Gene expression databases

BgeeiENSG00000169594.
CleanExiHS_BNC1.
ExpressionAtlasiQ01954. baseline and differential.
GenevisibleiQ01954. HS.

Organism-specific databases

HPAiCAB037339.

Interactioni

Subunit structurei

Interacts with HSF2BP (via C-terminus).By similarity

Protein-protein interaction databases

BioGridi107115. 1 interactor.
STRINGi9606.ENSP00000307041.

Structurei

3D structure databases

ProteinModelPortaliQ01954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni240 – 249Hydrophobic10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi533 – 539Nuclear localization signal1 Publication7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi859 – 876Ser-richAdd BLAST18

Sequence similaritiesi

Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri357 – 380C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri385 – 414C2H2-type 2PROSITE-ProRule annotationAdd BLAST30
Zinc fingeri720 – 743C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri748 – 775C2H2-type 4PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri928 – 951C2H2-type 5PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri956 – 983C2H2-type 6PROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IE3V. Eukaryota.
ENOG410YN2D. LUCA.
GeneTreeiENSGT00390000005844.
HOGENOMiHOG000049098.
HOVERGENiHBG050706.
InParanoidiQ01954.
OMAiEYPICVL.
OrthoDBiEOG091G01CA.
PhylomeDBiQ01954.
TreeFamiTF350399.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRRPPSRGG RGAARARETR RQPRHRSGRR MAEAISCTLN CSCQSFKPGK
60 70 80 90 100
INHRQCDQCK HGWVAHALSK LRIPPMYPTS QVEIVQSNVV FDISSLMLYG
110 120 130 140 150
TQAIPVRLKI LLDRLFSVLK QDEVLQILHA LDWTLQDYIR GYVLQDASGK
160 170 180 190 200
VLDHWSIMTS EEEVATLQQF LRFGETKSIV ELMAIQEKEE QSIIIPPSTA
210 220 230 240 250
NVDIRAFIES CSHRSSSLPT PVDKGNPSSI HPFENLISNM TFMLPFQFFN
260 270 280 290 300
PLPPALIGSL PEQYMLEQGH DQSQDPKQEV HGPFPDSSFL TSSSTPFQVE
310 320 330 340 350
KDQCLNCPDA ITKKEDSTHL SDSSSYNIVT KFERTQLSPE AKVKPERNSL
360 370 380 390 400
GTKKGRVFCT ACEKTFYDKG TLKIHYNAVH LKIKHKCTIE GCNMVFSSLR
410 420 430 440 450
SRNRHSANPN PRLHMPMNRN NRDKDLRNSL NLASSENYKC PGFTVTSPDC
460 470 480 490 500
RPPPSYPGSG EDSKGQPAFP NIGQNGVLFP NLKTVQPVLP FYRSPATPAE
510 520 530 540 550
VANTPGILPS LPLLSSSIPE QLISNEMPFD ALPKKKSRKS SMPIKIEKEA
560 570 580 590 600
VEIANEKRHN LSSDEDMPLQ VVSEDEQEAC SPQSHRVSEE QHVQSGGLGK
610 620 630 640 650
PFPEGERPCH RESVIESSGA ISQTPEQATH NSERETEQTP ALIMVPREVE
660 670 680 690 700
DGGHEHYFTP GMEPQVPFSD YMELQQRLLA GGLFSALSNR GMAFPCLEDS
710 720 730 740 750
KELEHVGQHA LARQIEENRF QCDICKKTFK NACSVKIHHK NMHVKEMHTC
760 770 780 790 800
TVEGCNATFP SRRSRDRHSS NLNLHQKALS QEALESSEDH FRAAYLLKDV
810 820 830 840 850
AKEAYQDVAF TQQASQTSVI FKGTSRMGSL VYPITQVHSA SLESYNSGPL
860 870 880 890 900
SEGTILDLST TSSMKSESSS HSSWDSDGVS EEGTVLMEDS DGNCEGSSLV
910 920 930 940 950
PGEDEYPICV LMEKADQSLA SLPSGLPITC HLCQKTYSNK GTFRAHYKTV
960 970 980 990
HLRQLHKCKV PGCNTMFSSV RSRNRHSQNP NLHKSLASSP SHLQ
Length:994
Mass (Da):110,972
Last modified:December 1, 2000 - v2
Checksum:i7DF13EE782E20ED5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 14PSRGGRGAA → EPGRTRGG in AAA35584 (PubMed:1332044).Curated9
Sequence conflicti156S → C in AAA35584 (PubMed:1332044).Curated1
Sequence conflicti284F → N in AAA35584 (PubMed:1332044).Curated1
Sequence conflicti401S → T in AAA35584 (PubMed:1332044).Curated1
Sequence conflicti638Q → H in AAA35584 (PubMed:1332044).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03427 mRNA. Translation: AAA35584.1.
U59694, U59692, U59693 Genomic DNA. Translation: AAB53028.1.
CCDSiCCDS10324.1.
PIRiA46415.
RefSeqiNP_001288135.1. NM_001301206.1.
NP_001708.3. NM_001717.3.
UniGeneiHs.459153.

Genome annotation databases

EnsembliENST00000345382; ENSP00000307041; ENSG00000169594.
GeneIDi646.
KEGGihsa:646.
UCSCiuc002bjt.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03427 mRNA. Translation: AAA35584.1.
U59694, U59692, U59693 Genomic DNA. Translation: AAB53028.1.
CCDSiCCDS10324.1.
PIRiA46415.
RefSeqiNP_001288135.1. NM_001301206.1.
NP_001708.3. NM_001717.3.
UniGeneiHs.459153.

3D structure databases

ProteinModelPortaliQ01954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107115. 1 interactor.
STRINGi9606.ENSP00000307041.

PTM databases

iPTMnetiQ01954.
PhosphoSitePlusiQ01954.

Polymorphism and mutation databases

BioMutaiBNC1.
DMDMi12644377.

Proteomic databases

EPDiQ01954.
MaxQBiQ01954.
PaxDbiQ01954.
PeptideAtlasiQ01954.
PRIDEiQ01954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000345382; ENSP00000307041; ENSG00000169594.
GeneIDi646.
KEGGihsa:646.
UCSCiuc002bjt.2. human.

Organism-specific databases

CTDi646.
DisGeNETi646.
GeneCardsiBNC1.
HGNCiHGNC:1081. BNC1.
HPAiCAB037339.
MIMi601930. gene.
neXtProtiNX_Q01954.
OpenTargetsiENSG00000169594.
PharmGKBiPA25391.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IE3V. Eukaryota.
ENOG410YN2D. LUCA.
GeneTreeiENSGT00390000005844.
HOGENOMiHOG000049098.
HOVERGENiHBG050706.
InParanoidiQ01954.
OMAiEYPICVL.
OrthoDBiEOG091G01CA.
PhylomeDBiQ01954.
TreeFamiTF350399.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169594-MONOMER.
SIGNORiQ01954.

Miscellaneous databases

GeneWikiiBNC1.
GenomeRNAii646.
PROiQ01954.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169594.
CleanExiHS_BNC1.
ExpressionAtlasiQ01954. baseline and differential.
GenevisibleiQ01954. HS.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBNC1_HUMAN
AccessioniPrimary (citable) accession number: Q01954
Secondary accession number(s): Q15840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.