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Q01954

- BNC1_HUMAN

UniProt

Q01954 - BNC1_HUMAN

Protein

Zinc finger protein basonuclin-1

Gene

BNC1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri357 – 38024C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri385 – 41430C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri720 – 74324C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri748 – 77528C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri928 – 95124C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri956 – 98328C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. chromosome organization Source: Ensembl
    2. epidermis development Source: ProtInc
    3. positive regulation of cell proliferation Source: ProtInc
    4. positive regulation of epithelial cell proliferation Source: Ensembl
    5. regulation of transcription from RNA polymerase II promoter Source: Ensembl
    6. regulation of transcription from RNA polymerase I promoter Source: Ensembl
    7. transcription, DNA-templated Source: UniProtKB-KW
    8. wound healing Source: Ensembl

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein basonuclin-1
    Gene namesi
    Name:BNC1
    Synonyms:BNC
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:1081. BNC1.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity
    Note: Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: ProtInc

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi537 – 5371S → A: No effect on phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-541. 1 Publication
    Mutagenesisi537 – 5371S → D: Reduces phosphorylation and induces partial relocation into the cytoplasm. 1 Publication
    Mutagenesisi540 – 5401S → D: No effect on phosphorylation, no effect on subcellular location. 1 Publication
    Mutagenesisi541 – 5411S → A: Strongly reduces phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-537. 1 Publication
    Mutagenesisi541 – 5411S → D: Strongly reduces phosphorylation and induces partial relocation into the cytoplasm. 1 Publication

    Organism-specific databases

    PharmGKBiPA25391.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 994994Zinc finger protein basonuclin-1PRO_0000046932Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei537 – 5371Phosphoserine1 Publication
    Modified residuei541 – 5411Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ01954.
    PaxDbiQ01954.
    PRIDEiQ01954.

    PTM databases

    PhosphoSiteiQ01954.

    Expressioni

    Tissue specificityi

    Expressed in epidermis, testis and foreskin, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart.1 Publication

    Gene expression databases

    ArrayExpressiQ01954.
    BgeeiQ01954.
    CleanExiHS_BNC1.
    GenevestigatoriQ01954.

    Interactioni

    Protein-protein interaction databases

    BioGridi107115. 1 interaction.
    STRINGi9606.ENSP00000307041.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01954.
    SMRiQ01954. Positions 347-420, 716-782, 918-959.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni240 – 24910Hydrophobic

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi533 – 5397Nuclear localization signal1 Publication

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi859 – 87618Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri357 – 38024C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri385 – 41430C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri720 – 74324C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri748 – 77528C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri928 – 95124C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri956 – 98328C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG82846.
    HOGENOMiHOG000049098.
    HOVERGENiHBG050706.
    InParanoidiQ01954.
    OMAiTLNCSCQ.
    OrthoDBiEOG7SV0TM.
    PhylomeDBiQ01954.
    TreeFamiTF350399.

    Family and domain databases

    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 6 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01954-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRRPPSRGG RGAARARETR RQPRHRSGRR MAEAISCTLN CSCQSFKPGK    50
    INHRQCDQCK HGWVAHALSK LRIPPMYPTS QVEIVQSNVV FDISSLMLYG 100
    TQAIPVRLKI LLDRLFSVLK QDEVLQILHA LDWTLQDYIR GYVLQDASGK 150
    VLDHWSIMTS EEEVATLQQF LRFGETKSIV ELMAIQEKEE QSIIIPPSTA 200
    NVDIRAFIES CSHRSSSLPT PVDKGNPSSI HPFENLISNM TFMLPFQFFN 250
    PLPPALIGSL PEQYMLEQGH DQSQDPKQEV HGPFPDSSFL TSSSTPFQVE 300
    KDQCLNCPDA ITKKEDSTHL SDSSSYNIVT KFERTQLSPE AKVKPERNSL 350
    GTKKGRVFCT ACEKTFYDKG TLKIHYNAVH LKIKHKCTIE GCNMVFSSLR 400
    SRNRHSANPN PRLHMPMNRN NRDKDLRNSL NLASSENYKC PGFTVTSPDC 450
    RPPPSYPGSG EDSKGQPAFP NIGQNGVLFP NLKTVQPVLP FYRSPATPAE 500
    VANTPGILPS LPLLSSSIPE QLISNEMPFD ALPKKKSRKS SMPIKIEKEA 550
    VEIANEKRHN LSSDEDMPLQ VVSEDEQEAC SPQSHRVSEE QHVQSGGLGK 600
    PFPEGERPCH RESVIESSGA ISQTPEQATH NSERETEQTP ALIMVPREVE 650
    DGGHEHYFTP GMEPQVPFSD YMELQQRLLA GGLFSALSNR GMAFPCLEDS 700
    KELEHVGQHA LARQIEENRF QCDICKKTFK NACSVKIHHK NMHVKEMHTC 750
    TVEGCNATFP SRRSRDRHSS NLNLHQKALS QEALESSEDH FRAAYLLKDV 800
    AKEAYQDVAF TQQASQTSVI FKGTSRMGSL VYPITQVHSA SLESYNSGPL 850
    SEGTILDLST TSSMKSESSS HSSWDSDGVS EEGTVLMEDS DGNCEGSSLV 900
    PGEDEYPICV LMEKADQSLA SLPSGLPITC HLCQKTYSNK GTFRAHYKTV 950
    HLRQLHKCKV PGCNTMFSSV RSRNRHSQNP NLHKSLASSP SHLQ 994
    Length:994
    Mass (Da):110,972
    Last modified:December 1, 2000 - v2
    Checksum:i7DF13EE782E20ED5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 149PSRGGRGAA → EPGRTRGG in AAA35584. (PubMed:1332044)Curated
    Sequence conflicti156 – 1561S → C in AAA35584. (PubMed:1332044)Curated
    Sequence conflicti284 – 2841F → N in AAA35584. (PubMed:1332044)Curated
    Sequence conflicti401 – 4011S → T in AAA35584. (PubMed:1332044)Curated
    Sequence conflicti638 – 6381Q → H in AAA35584. (PubMed:1332044)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03427 mRNA. Translation: AAA35584.1.
    U59694, U59692, U59693 Genomic DNA. Translation: AAB53028.1.
    CCDSiCCDS10324.1.
    PIRiA46415.
    RefSeqiNP_001708.3. NM_001717.3.
    UniGeneiHs.459153.

    Genome annotation databases

    EnsembliENST00000345382; ENSP00000307041; ENSG00000169594.
    GeneIDi646.
    KEGGihsa:646.
    UCSCiuc002bjt.1. human.

    Polymorphism databases

    DMDMi12644377.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03427 mRNA. Translation: AAA35584.1 .
    U59694 , U59692 , U59693 Genomic DNA. Translation: AAB53028.1 .
    CCDSi CCDS10324.1.
    PIRi A46415.
    RefSeqi NP_001708.3. NM_001717.3.
    UniGenei Hs.459153.

    3D structure databases

    ProteinModelPortali Q01954.
    SMRi Q01954. Positions 347-420, 716-782, 918-959.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107115. 1 interaction.
    STRINGi 9606.ENSP00000307041.

    PTM databases

    PhosphoSitei Q01954.

    Polymorphism databases

    DMDMi 12644377.

    Proteomic databases

    MaxQBi Q01954.
    PaxDbi Q01954.
    PRIDEi Q01954.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000345382 ; ENSP00000307041 ; ENSG00000169594 .
    GeneIDi 646.
    KEGGi hsa:646.
    UCSCi uc002bjt.1. human.

    Organism-specific databases

    CTDi 646.
    GeneCardsi GC15M083924.
    HGNCi HGNC:1081. BNC1.
    MIMi 601930. gene.
    neXtProti NX_Q01954.
    PharmGKBi PA25391.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG82846.
    HOGENOMi HOG000049098.
    HOVERGENi HBG050706.
    InParanoidi Q01954.
    OMAi TLNCSCQ.
    OrthoDBi EOG7SV0TM.
    PhylomeDBi Q01954.
    TreeFami TF350399.

    Miscellaneous databases

    GeneWikii BNC1.
    GenomeRNAii 646.
    NextBioi 2622.
    PROi Q01954.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q01954.
    Bgeei Q01954.
    CleanExi HS_BNC1.
    Genevestigatori Q01954.

    Family and domain databases

    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 6 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Basonuclin: a keratinocyte protein with multiple paired zinc fingers."
      Tseng H., Green H.
      Proc. Natl. Acad. Sci. U.S.A. 89:10311-10315(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Keratinocyte.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Nuclear localization of basonuclin in human keratinocytes and the role of phosphorylation."
      Iuchi S., Green H.
      Proc. Natl. Acad. Sci. U.S.A. 94:7948-7953(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, PHOSPHORYLATION AT SER-537 AND SER-541, MUTAGENESIS OF SER-537; SER-540 AND SER-541.
    4. "Translocation of the zinc finger protein basonuclin from the mouse germ cell nucleus to the midpiece of the spermatozoon during spermiogenesis."
      Mahoney M.G., Tang W., Xiang M.M., Moss S.B., Gerton G.L., Stanley J.R., Tseng H.
      Biol. Reprod. 59:388-394(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiBNC1_HUMAN
    AccessioniPrimary (citable) accession number: Q01954
    Secondary accession number(s): Q15840
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3