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Q01954 (BNC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein basonuclin-1
Gene names
Name:BNC1
Synonyms:BNC
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length994 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Note: Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids By similarity. Ref.3

Tissue specificity

Expressed in epidermis, testis and foreskin, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart. Ref.4

Post-translational modification

Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.

Sequence similarities

Contains 6 C2H2-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 994994Zinc finger protein basonuclin-1
PRO_0000046932

Regions

Zinc finger357 – 38024C2H2-type 1
Zinc finger385 – 41430C2H2-type 2
Zinc finger720 – 74324C2H2-type 3
Zinc finger748 – 77528C2H2-type 4
Zinc finger928 – 95124C2H2-type 5
Zinc finger956 – 98328C2H2-type 6
Region240 – 24910Hydrophobic
Motif533 – 5397Nuclear localization signal Ref.3
Compositional bias859 – 87618Ser-rich

Amino acid modifications

Modified residue5371Phosphoserine Ref.3
Modified residue5411Phosphoserine Ref.3

Experimental info

Mutagenesis5371S → A: No effect on phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-541. Ref.3
Mutagenesis5371S → D: Reduces phosphorylation and induces partial relocation into the cytoplasm. Ref.3
Mutagenesis5401S → D: No effect on phosphorylation, no effect on subcellular location. Ref.3
Mutagenesis5411S → A: Strongly reduces phosphorylation. Abolishes phosphorylation and induces nuclear restriction; when associated with A-537. Ref.3
Mutagenesis5411S → D: Strongly reduces phosphorylation and induces partial relocation into the cytoplasm. Ref.3
Sequence conflict6 – 149PSRGGRGAA → EPGRTRGG in AAA35584. Ref.1
Sequence conflict1561S → C in AAA35584. Ref.1
Sequence conflict2841F → N in AAA35584. Ref.1
Sequence conflict4011S → T in AAA35584. Ref.1
Sequence conflict6381Q → H in AAA35584. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q01954 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 7DF13EE782E20ED5

FASTA994110,972
        10         20         30         40         50         60 
MRRRPPSRGG RGAARARETR RQPRHRSGRR MAEAISCTLN CSCQSFKPGK INHRQCDQCK 

        70         80         90        100        110        120 
HGWVAHALSK LRIPPMYPTS QVEIVQSNVV FDISSLMLYG TQAIPVRLKI LLDRLFSVLK 

       130        140        150        160        170        180 
QDEVLQILHA LDWTLQDYIR GYVLQDASGK VLDHWSIMTS EEEVATLQQF LRFGETKSIV 

       190        200        210        220        230        240 
ELMAIQEKEE QSIIIPPSTA NVDIRAFIES CSHRSSSLPT PVDKGNPSSI HPFENLISNM 

       250        260        270        280        290        300 
TFMLPFQFFN PLPPALIGSL PEQYMLEQGH DQSQDPKQEV HGPFPDSSFL TSSSTPFQVE 

       310        320        330        340        350        360 
KDQCLNCPDA ITKKEDSTHL SDSSSYNIVT KFERTQLSPE AKVKPERNSL GTKKGRVFCT 

       370        380        390        400        410        420 
ACEKTFYDKG TLKIHYNAVH LKIKHKCTIE GCNMVFSSLR SRNRHSANPN PRLHMPMNRN 

       430        440        450        460        470        480 
NRDKDLRNSL NLASSENYKC PGFTVTSPDC RPPPSYPGSG EDSKGQPAFP NIGQNGVLFP 

       490        500        510        520        530        540 
NLKTVQPVLP FYRSPATPAE VANTPGILPS LPLLSSSIPE QLISNEMPFD ALPKKKSRKS 

       550        560        570        580        590        600 
SMPIKIEKEA VEIANEKRHN LSSDEDMPLQ VVSEDEQEAC SPQSHRVSEE QHVQSGGLGK 

       610        620        630        640        650        660 
PFPEGERPCH RESVIESSGA ISQTPEQATH NSERETEQTP ALIMVPREVE DGGHEHYFTP 

       670        680        690        700        710        720 
GMEPQVPFSD YMELQQRLLA GGLFSALSNR GMAFPCLEDS KELEHVGQHA LARQIEENRF 

       730        740        750        760        770        780 
QCDICKKTFK NACSVKIHHK NMHVKEMHTC TVEGCNATFP SRRSRDRHSS NLNLHQKALS 

       790        800        810        820        830        840 
QEALESSEDH FRAAYLLKDV AKEAYQDVAF TQQASQTSVI FKGTSRMGSL VYPITQVHSA 

       850        860        870        880        890        900 
SLESYNSGPL SEGTILDLST TSSMKSESSS HSSWDSDGVS EEGTVLMEDS DGNCEGSSLV 

       910        920        930        940        950        960 
PGEDEYPICV LMEKADQSLA SLPSGLPITC HLCQKTYSNK GTFRAHYKTV HLRQLHKCKV 

       970        980        990 
PGCNTMFSSV RSRNRHSQNP NLHKSLASSP SHLQ 

« Hide

References

[1]"Basonuclin: a keratinocyte protein with multiple paired zinc fingers."
Tseng H., Green H.
Proc. Natl. Acad. Sci. U.S.A. 89:10311-10315(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Keratinocyte.
[2]"The human basonuclin gene."
Teumer J., Tseng H., Green H.
Gene 188:1-7(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Nuclear localization of basonuclin in human keratinocytes and the role of phosphorylation."
Iuchi S., Green H.
Proc. Natl. Acad. Sci. U.S.A. 94:7948-7953(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, PHOSPHORYLATION AT SER-537 AND SER-541, MUTAGENESIS OF SER-537; SER-540 AND SER-541.
[4]"Translocation of the zinc finger protein basonuclin from the mouse germ cell nucleus to the midpiece of the spermatozoon during spermiogenesis."
Mahoney M.G., Tang W., Xiang M.M., Moss S.B., Gerton G.L., Stanley J.R., Tseng H.
Biol. Reprod. 59:388-394(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L03427 mRNA. Translation: AAA35584.1.
U59694, U59692, U59693 Genomic DNA. Translation: AAB53028.1.
PIRA46415.
RefSeqNP_001708.3. NM_001717.3.
UniGeneHs.459153.

3D structure databases

ProteinModelPortalQ01954.
SMRQ01954. Positions 352-406, 717-768, 918-959.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107115. 1 interaction.
STRING9606.ENSP00000307041.

PTM databases

PhosphoSiteQ01954.

Polymorphism databases

DMDM12644377.

Proteomic databases

PaxDbQ01954.
PRIDEQ01954.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000345382; ENSP00000307041; ENSG00000169594.
GeneID646.
KEGGhsa:646.
UCSCuc002bjt.1. human.

Organism-specific databases

CTD646.
GeneCardsGC15M083924.
HGNCHGNC:1081. BNC1.
MIM601930. gene.
neXtProtNX_Q01954.
PharmGKBPA25391.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG82846.
HOGENOMHOG000049098.
HOVERGENHBG050706.
InParanoidQ01954.
OMAMEKADQS.
OrthoDBEOG7SV0TM.
PhylomeDBQ01954.
TreeFamTF350399.

Gene expression databases

ArrayExpressQ01954.
BgeeQ01954.
CleanExHS_BNC1.
GenevestigatorQ01954.

Family and domain databases

InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiBNC1.
GenomeRNAi646.
NextBio2622.
PROQ01954.
SOURCESearch...

Entry information

Entry nameBNC1_HUMAN
AccessionPrimary (citable) accession number: Q01954
Secondary accession number(s): Q15840
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 1, 2000
Last modified: March 19, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM