Reviewed, UniProtKB/Swiss-Prot Q01930 (PUR6_PICME)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:

ADE1

Organism

Pichia methanolica (Yeast)

Taxonomic identifier

33166 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Sequence similarities	In the C-terminal section; belongs to the AIR carboxylase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Phosphoribosylaminoimidazole carboxylase		PRO_0000075025
Regions
	Domain	110 – 297	188	ATP-grasp
	Nucleotide binding	137 – 192	56	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01930-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 852AADBB8B60EFA3	FASTA	543	58,544
	10 20 30 40 50 60 MDSQTVGILG GGQLGRMIVE AAHRLNIKTV ILENGDQAPA KQINALDDHI DGSFNDPKAI 70 80 90 100 110 120 AELAAKCDVL TVEIEHVDTD ALVEVQKATG IKIFPSPETI SLIKDKYLQK EHLIKNGIAV 130 140 150 160 170 180 AESCSVESSA ASLEEVGAKY GFPYMLKSRT MAYDGRGNFV VKDKSYIPEA LKVLDDRPLY 190 200 210 220 230 240 AEKWAPFSKE LAVMVVRSID GQVYSYPTVE TIHQNNICHT VFAPARVNDT VQKKAQILAD 250 260 270 280 290 300 NAVKSFPGAG IFGVEMFLLQ NGDLLVNEIA PRPHNSGHYT IDACVTSQFE AHVRAITGLP 310 320 330 340 350 360 MPKNFTCLST PSTQAIMLNV LGGDEQNGEF KMCKRALETP HASVYLYGKT TRPGRKMGHI 370 380 390 400 410 420 NIVSQSMTDC ERRLHYIEGT TNSIPLEEQY TTDSIPGTSS KPLVGVIMGS DSDLPVMSLG 430 440 450 460 470 480 CNILKQFNVP FEVTIVSAHR TPQRMAKYAI DAPKRGLKCI IAGAGGAAHL PGMVAAMTPL 490 500 510 520 530 540 PVIGVPVKGS TLDGVDSLHS IVQMPRGIPV ATVAINNATN AALLAITILG AGDPNTCLQW KFI

References

[1]

"The 5-aminoimidazole ribonucleotide-carboxylase structural gene of the methylotrophic yeast Pichia methanolica: cloning, sequencing and homology analysis." Hiep T.T., Kulikov V.N., Noskov V.N., Sizonenko G.I., Chernoff Y.O., Pavlov Y.I. Yeast 9:1251-1258(1993) [PubMed: 8109174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76529 Genomic DNA. Translation: CAA54041.1.
PIR	S39112.
3D structure databases
HSSP	HSSP built from PDB template 1O4V based on UniProtKB Q9WYS7.
SMR	Q01930. Positions 402-534.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.1.1.21. 275792.
Family and domain databases
InterPro	IPR016301. AIR_COase. IPR005875. AIR_COase_ATPase-su. IPR000031. AIR_COase_core. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF00731. AIRC. 1 hit. PF02222. ATP-grasp. 1 hit. [Graphical view]
PIRSF	PIRSF001340. AIR_carboxylase. 1 hit.
ProDom	PD002193. AIR_carboxyl. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01162. purE. 1 hit. TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR6_PICME

Accession

Primary (citable) accession number: Q01930

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 1, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)