Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q01926 (MRS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Magnesium transporter MRS2, mitochondrial
Alternative name(s):
RNA-splicing protein MRS2
Gene names
Name:MRS2
Ordered Locus Names:YOR334W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes. Ref.6 Ref.8 Ref.11 Ref.12

Subunit structure

Forms homooligomers. Interacts with MFM1. Ref.12

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Ref.9.

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family. [View classification]

Caution

Was originally (Ref.1 and Ref.2) identified in genetic screens for bona fide RNA splicing factors, but it has later been shown that not the MRS2 protein per se but certain magnesium concentrations are essential for group II intron splicing (Ref.6).

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MFM1Q027832EBI-11283,EBI-33672

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3232Mitochondrion Potential
Chain33 – 470438Magnesium transporter MRS2, mitochondrial
PRO_0000021753

Regions

Topological domain33 – 314282Mitochondrial matrix Potential
Transmembrane315 – 33319Helical; Potential
Topological domain334 – 34411Mitochondrial intermembrane Potential
Transmembrane345 – 36117Helical; Potential
Topological domain362 – 470109Mitochondrial matrix Potential

Experimental info

Sequence conflict3711S → F in AAA34795. Ref.1
Sequence conflict3711S → F Ref.2
Sequence conflict4481E → D in AAA34795. Ref.1
Sequence conflict4481E → D Ref.2

Secondary structure

........................... 470
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q01926 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: EFEBA11765AB8B31

FASTA47054,203
        10         20         30         40         50         60 
MNRRLLVRSI SCFQPLSRIT FGRPNTPFLR KYADTSTAAN TNSTILRKQL LSLKPISASD 

        70         80         90        100        110        120 
SLFISCTVFN SKGNIISMSE KFPKWSFLTE HSLFPRDLRK IDNSSIDIIP TIMCKPNCIV 

       130        140        150        160        170        180 
INLLHIKALI ERDKVYVFDT TNPSAAAKLS VLMYDLESKL SSTKNNSQFY EHRALESIFI 

       190        200        210        220        230        240 
NVMSALETDF KLHSQICIQI LNDLENEVNR LKLRHLLIKS KDLTLFYQKT LLIRDLLDEL 

       250        260        270        280        290        300 
LENDDDLANM YLTVKKSPKD NFSDLEMLIE TYYTQCDEYV QQSESLIQDI KSTEEIVNII 

       310        320        330        340        350        360 
LDANRNSLML LELKVTIYTL GFTVASVLPA FYGMNLKNFI EESEWGFTSV AVFSIVSALY 

       370        380        390        400        410        420 
ITKKNFNSLR SVTKMTMYPN SPANSSVYPK TSASIALTNK LKRRRKWWKS TKQRLGVLLY 

       430        440        450        460        470 
GSSYTNKANL SNNKINKGFS KVKKFNMEND IKNKQNRDMI WKWLIEDKKN 

« Hide

References

« Hide 'large scale' references
[1]"The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo."
Wiesenberger G., Waldherr M., Schweyen R.J.
J. Biol. Chem. 267:6963-6969(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 44774 / DBY747.
[2]"A multitude of suppressors of group II intron-splicing defects in yeast."
Waldherr M., Ragnini A., Jank B., Teply R., Wiesenberger G., Schweyen R.J.
Curr. Genet. 24:301-306(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I."
Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H.
Yeast 12:999-1004(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo."
Gregan J., Kolisek M., Schweyen R.J.
Genes Dev. 15:2229-2237(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Insertion of bitopic membrane proteins into the inner membrane of mitochondria involves an export step from the matrix."
Baumann F., Neupert W., Herrmann J.M.
J. Biol. Chem. 277:21405-21413(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
[8]"Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria."
Kolisek M., Zsurka G., Samaj J., Weghuber J., Schweyen R.J., Schweigel M.
EMBO J. 22:1235-1244(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Mrs2p forms a high conductance Mg2+ selective channel in mitochondria."
Schindl R., Weghuber J., Romanin C., Schweyen R.J.
Biophys. J. 93:3872-3883(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+ channel."
Sponder G., Svidova S., Schindl R., Wieser S., Schweyen R.J., Romanin C., Froschauer E.M., Weghuber J.
FEBS J. 277:3514-3525(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MFM1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M82916 Genomic DNA. Translation: AAA34795.1.
Z49821 Genomic DNA. Translation: CAA89979.1.
Z75241 Genomic DNA. Translation: CAA99656.1.
BK006948 Genomic DNA. Translation: DAA11095.1.
PIRS62064.
RefSeqNP_014979.1. NM_001183754.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RKGX-ray1.28A48-308[»]
ProteinModelPortalQ01926.
SMRQ01926. Positions 48-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34717. 59 interactions.
DIPDIP-5325N.
IntActQ01926. 1 interaction.
MINTMINT-553075.
STRING4932.YOR334W.

Protein family/group databases

TCDB1.A.35.5.1. the cora metal ion transporter (mit) family.

Proteomic databases

MaxQBQ01926.
PaxDbQ01926.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR334W; YOR334W; YOR334W.
GeneID854511.
KEGGsce:YOR334W.

Organism-specific databases

CYGDYOR334w.
SGDS000005861. MRS2.

Phylogenomic databases

eggNOGNOG284534.
GeneTreeENSGT00390000009988.
HOGENOMHOG000248323.
KOK16075.
OMACDEYVQQ.
OrthoDBEOG7SXWCP.

Enzyme and pathway databases

BioCycYEAST:G3O-33809-MONOMER.

Gene expression databases

GenevestigatorQ01926.

Family and domain databases

InterProIPR026573. MRS2/LPE10.
[Graphical view]
PANTHERPTHR13890. PTHR13890. 1 hit.
ProtoNetSearch...

Other

NextBio976867.

Entry information

Entry nameMRS2_YEAST
AccessionPrimary (citable) accession number: Q01926
Secondary accession number(s): D6W329
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references