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Protein

Magnesium transporter MRS2, mitochondrial

Gene

MRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.4 Publications

GO - Molecular functioni

  • magnesium ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • mitochondrial magnesium ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciYEAST:G3O-33809-MONOMER.
ReactomeiREACT_350279. Miscellaneous transport and binding events.

Protein family/group databases

TCDBi1.A.35.5.1. the cora metal ion transporter (mit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium transporter MRS2, mitochondrial
Alternative name(s):
RNA-splicing protein MRS2
Gene namesi
Name:MRS2
Ordered Locus Names:YOR334W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR334w.
EuPathDBiFungiDB:YOR334W.
SGDiS000005861. MRS2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 314282Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei315 – 33319HelicalSequence AnalysisAdd
BLAST
Topological domaini334 – 34411Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei345 – 36117HelicalSequence AnalysisAdd
BLAST
Topological domaini362 – 470109Mitochondrial matrixSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232MitochondrionSequence AnalysisAdd
BLAST
Chaini33 – 470438Magnesium transporter MRS2, mitochondrialPRO_0000021753Add
BLAST

Proteomic databases

MaxQBiQ01926.
PaxDbiQ01926.

Interactioni

Subunit structurei

Forms homooligomers. Interacts with MFM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MFM1Q027832EBI-11283,EBI-33672

Protein-protein interaction databases

BioGridi34717. 61 interactions.
DIPiDIP-5325N.
IntActiQ01926. 1 interaction.
MINTiMINT-553075.

Structurei

Secondary structure

1
470
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi59 – 624Combined sources
Beta strandi63 – 697Combined sources
Beta strandi75 – 839Combined sources
Helixi84 – 907Combined sources
Helixi95 – 1006Combined sources
Beta strandi111 – 1144Combined sources
Beta strandi119 – 1235Combined sources
Beta strandi126 – 1305Combined sources
Beta strandi135 – 1384Combined sources
Helixi143 – 16119Combined sources
Helixi170 – 20637Combined sources
Helixi210 – 24132Combined sources
Helixi244 – 2496Combined sources
Helixi263 – 30341Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RKGX-ray1.28A48-308[»]
ProteinModelPortaliQ01926.
SMRiQ01926. Positions 48-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG284534.
GeneTreeiENSGT00390000009988.
HOGENOMiHOG000248323.
InParanoidiQ01926.
KOiK16075.
OMAiWRMINDD.
OrthoDBiEOG7SXWCP.

Family and domain databases

InterProiIPR026573. MRS2/LPE10.
[Graphical view]
PANTHERiPTHR13890. PTHR13890. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01926-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRLLVRSI SCFQPLSRIT FGRPNTPFLR KYADTSTAAN TNSTILRKQL
60 70 80 90 100
LSLKPISASD SLFISCTVFN SKGNIISMSE KFPKWSFLTE HSLFPRDLRK
110 120 130 140 150
IDNSSIDIIP TIMCKPNCIV INLLHIKALI ERDKVYVFDT TNPSAAAKLS
160 170 180 190 200
VLMYDLESKL SSTKNNSQFY EHRALESIFI NVMSALETDF KLHSQICIQI
210 220 230 240 250
LNDLENEVNR LKLRHLLIKS KDLTLFYQKT LLIRDLLDEL LENDDDLANM
260 270 280 290 300
YLTVKKSPKD NFSDLEMLIE TYYTQCDEYV QQSESLIQDI KSTEEIVNII
310 320 330 340 350
LDANRNSLML LELKVTIYTL GFTVASVLPA FYGMNLKNFI EESEWGFTSV
360 370 380 390 400
AVFSIVSALY ITKKNFNSLR SVTKMTMYPN SPANSSVYPK TSASIALTNK
410 420 430 440 450
LKRRRKWWKS TKQRLGVLLY GSSYTNKANL SNNKINKGFS KVKKFNMEND
460 470
IKNKQNRDMI WKWLIEDKKN
Length:470
Mass (Da):54,203
Last modified:October 1, 1996 - v2
Checksum:iEFEBA11765AB8B31
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti371 – 3711S → F in AAA34795 (PubMed:1551905).Curated
Sequence conflicti371 – 3711S → F (PubMed:8252639).Curated
Sequence conflicti448 – 4481E → D in AAA34795 (PubMed:1551905).Curated
Sequence conflicti448 – 4481E → D (PubMed:8252639).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82916 Genomic DNA. Translation: AAA34795.1.
Z49821 Genomic DNA. Translation: CAA89979.1.
Z75241 Genomic DNA. Translation: CAA99656.1.
BK006948 Genomic DNA. Translation: DAA11095.1.
PIRiS62064.
RefSeqiNP_014979.1. NM_001183754.1.

Genome annotation databases

EnsemblFungiiYOR334W; YOR334W; YOR334W.
GeneIDi854511.
KEGGisce:YOR334W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82916 Genomic DNA. Translation: AAA34795.1.
Z49821 Genomic DNA. Translation: CAA89979.1.
Z75241 Genomic DNA. Translation: CAA99656.1.
BK006948 Genomic DNA. Translation: DAA11095.1.
PIRiS62064.
RefSeqiNP_014979.1. NM_001183754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RKGX-ray1.28A48-308[»]
ProteinModelPortaliQ01926.
SMRiQ01926. Positions 48-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34717. 61 interactions.
DIPiDIP-5325N.
IntActiQ01926. 1 interaction.
MINTiMINT-553075.

Protein family/group databases

TCDBi1.A.35.5.1. the cora metal ion transporter (mit) family.

Proteomic databases

MaxQBiQ01926.
PaxDbiQ01926.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR334W; YOR334W; YOR334W.
GeneIDi854511.
KEGGisce:YOR334W.

Organism-specific databases

CYGDiYOR334w.
EuPathDBiFungiDB:YOR334W.
SGDiS000005861. MRS2.

Phylogenomic databases

eggNOGiNOG284534.
GeneTreeiENSGT00390000009988.
HOGENOMiHOG000248323.
InParanoidiQ01926.
KOiK16075.
OMAiWRMINDD.
OrthoDBiEOG7SXWCP.

Enzyme and pathway databases

BioCyciYEAST:G3O-33809-MONOMER.
ReactomeiREACT_350279. Miscellaneous transport and binding events.

Miscellaneous databases

NextBioi976867.
PROiQ01926.

Family and domain databases

InterProiIPR026573. MRS2/LPE10.
[Graphical view]
PANTHERiPTHR13890. PTHR13890. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo."
    Wiesenberger G., Waldherr M., Schweyen R.J.
    J. Biol. Chem. 267:6963-6969(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 44774 / DBY747.
  2. "A multitude of suppressors of group II intron-splicing defects in yeast."
    Waldherr M., Ragnini A., Jank B., Teply R., Wiesenberger G., Schweyen R.J.
    Curr. Genet. 24:301-306(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I."
    Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H.
    Yeast 12:999-1004(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo."
    Gregan J., Kolisek M., Schweyen R.J.
    Genes Dev. 15:2229-2237(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Insertion of bitopic membrane proteins into the inner membrane of mitochondria involves an export step from the matrix."
    Baumann F., Neupert W., Herrmann J.M.
    J. Biol. Chem. 277:21405-21413(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  8. "Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria."
    Kolisek M., Zsurka G., Samaj J., Weghuber J., Schweyen R.J., Schweigel M.
    EMBO J. 22:1235-1244(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Mrs2p forms a high conductance Mg2+ selective channel in mitochondria."
    Schindl R., Weghuber J., Romanin C., Schweyen R.J.
    Biophys. J. 93:3872-3883(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+ channel."
    Sponder G., Svidova S., Schindl R., Wieser S., Schweyen R.J., Romanin C., Froschauer E.M., Weghuber J.
    FEBS J. 277:3514-3525(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MFM1.

Entry informationi

Entry nameiMRS2_YEAST
AccessioniPrimary (citable) accession number: Q01926
Secondary accession number(s): D6W329
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally (PubMed:1551905 and PubMed:8252639) identified in genetic screens for bona fide RNA splicing factors, but it has later been shown that not the MRS2 protein per se but certain magnesium concentrations are essential for group II intron splicing.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.