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Protein

Magnesium transporter MRS2, mitochondrial

Gene

MRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix (PubMed:12628916, PubMed:17827224, PubMed:20653776, PubMed:23999289). Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes (PubMed:11544180).5 Publications

GO - Molecular functioni

  • magnesium ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • mitochondrial magnesium ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciYEAST:G3O-33809-MONOMER.
ReactomeiR-SCE-5223345. Miscellaneous transport and binding events.

Protein family/group databases

TCDBi1.A.35.5.1. the cora metal ion transporter (mit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium transporter MRS2, mitochondrial
Alternative name(s):
RNA-splicing protein MRS2
Gene namesi
Name:MRS2
Ordered Locus Names:YOR334W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR334W.
SGDiS000005861. MRS2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 314Mitochondrial matrixSequence analysisAdd BLAST282
Transmembranei315 – 333HelicalSequence analysisAdd BLAST19
Topological domaini334 – 344Mitochondrial intermembraneSequence analysisAdd BLAST11
Transmembranei345 – 361HelicalSequence analysisAdd BLAST17
Topological domaini362 – 470Mitochondrial matrixSequence analysisAdd BLAST109

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97D → A, F or W: No effect on Mg(2+) import. 1 Publication1
Mutagenesisi309M → E or G: Increases Mg(2+) import into mitochondria. 1 Publication1
Mutagenesisi309M → F: Decreases Mg(2+) import into mitochondria. 1 Publication1
Mutagenesisi315V → E, F or G: No effect on Mg(2+) import. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionSequence analysisAdd BLAST32
ChainiPRO_000002175333 – 470Magnesium transporter MRS2, mitochondrialAdd BLAST438

Proteomic databases

MaxQBiQ01926.
PRIDEiQ01926.

Interactioni

Subunit structurei

Homopentamer (PubMed:23999289). Forms homooligomers. Interacts with MFM1 (PubMed:20653776).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MFM1Q027832EBI-11283,EBI-33672

Protein-protein interaction databases

BioGridi34717. 62 interactors.
DIPiDIP-5325N.
IntActiQ01926. 1 interactor.
MINTiMINT-553075.

Structurei

Secondary structure

1470
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi59 – 62Combined sources4
Beta strandi63 – 69Combined sources7
Beta strandi75 – 83Combined sources9
Helixi84 – 90Combined sources7
Helixi95 – 100Combined sources6
Beta strandi111 – 114Combined sources4
Beta strandi119 – 123Combined sources5
Beta strandi126 – 130Combined sources5
Beta strandi135 – 138Combined sources4
Helixi143 – 161Combined sources19
Helixi170 – 206Combined sources37
Helixi210 – 241Combined sources32
Helixi244 – 249Combined sources6
Helixi263 – 303Combined sources41

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RKGX-ray1.28A48-308[»]
ProteinModelPortaliQ01926.
SMRiQ01926.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi332 – 335YGMNCurated4

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000009988.
HOGENOMiHOG000248323.
InParanoidiQ01926.
KOiK16075.
OMAiRDMIWKW.
OrthoDBiEOG092C23OZ.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01926-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRLLVRSI SCFQPLSRIT FGRPNTPFLR KYADTSTAAN TNSTILRKQL
60 70 80 90 100
LSLKPISASD SLFISCTVFN SKGNIISMSE KFPKWSFLTE HSLFPRDLRK
110 120 130 140 150
IDNSSIDIIP TIMCKPNCIV INLLHIKALI ERDKVYVFDT TNPSAAAKLS
160 170 180 190 200
VLMYDLESKL SSTKNNSQFY EHRALESIFI NVMSALETDF KLHSQICIQI
210 220 230 240 250
LNDLENEVNR LKLRHLLIKS KDLTLFYQKT LLIRDLLDEL LENDDDLANM
260 270 280 290 300
YLTVKKSPKD NFSDLEMLIE TYYTQCDEYV QQSESLIQDI KSTEEIVNII
310 320 330 340 350
LDANRNSLML LELKVTIYTL GFTVASVLPA FYGMNLKNFI EESEWGFTSV
360 370 380 390 400
AVFSIVSALY ITKKNFNSLR SVTKMTMYPN SPANSSVYPK TSASIALTNK
410 420 430 440 450
LKRRRKWWKS TKQRLGVLLY GSSYTNKANL SNNKINKGFS KVKKFNMEND
460 470
IKNKQNRDMI WKWLIEDKKN
Length:470
Mass (Da):54,203
Last modified:October 1, 1996 - v2
Checksum:iEFEBA11765AB8B31
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti371S → F in AAA34795 (PubMed:1551905).Curated1
Sequence conflicti371S → F (PubMed:8252639).Curated1
Sequence conflicti448E → D in AAA34795 (PubMed:1551905).Curated1
Sequence conflicti448E → D (PubMed:8252639).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82916 Genomic DNA. Translation: AAA34795.1.
Z49821 Genomic DNA. Translation: CAA89979.1.
Z75241 Genomic DNA. Translation: CAA99656.1.
BK006948 Genomic DNA. Translation: DAA11095.1.
PIRiS62064.
RefSeqiNP_014979.1. NM_001183754.1.

Genome annotation databases

EnsemblFungiiYOR334W; YOR334W; YOR334W.
GeneIDi854511.
KEGGisce:YOR334W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82916 Genomic DNA. Translation: AAA34795.1.
Z49821 Genomic DNA. Translation: CAA89979.1.
Z75241 Genomic DNA. Translation: CAA99656.1.
BK006948 Genomic DNA. Translation: DAA11095.1.
PIRiS62064.
RefSeqiNP_014979.1. NM_001183754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RKGX-ray1.28A48-308[»]
ProteinModelPortaliQ01926.
SMRiQ01926.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34717. 62 interactors.
DIPiDIP-5325N.
IntActiQ01926. 1 interactor.
MINTiMINT-553075.

Protein family/group databases

TCDBi1.A.35.5.1. the cora metal ion transporter (mit) family.

Proteomic databases

MaxQBiQ01926.
PRIDEiQ01926.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR334W; YOR334W; YOR334W.
GeneIDi854511.
KEGGisce:YOR334W.

Organism-specific databases

EuPathDBiFungiDB:YOR334W.
SGDiS000005861. MRS2.

Phylogenomic databases

GeneTreeiENSGT00390000009988.
HOGENOMiHOG000248323.
InParanoidiQ01926.
KOiK16075.
OMAiRDMIWKW.
OrthoDBiEOG092C23OZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-33809-MONOMER.
ReactomeiR-SCE-5223345. Miscellaneous transport and binding events.

Miscellaneous databases

PROiQ01926.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiMRS2_YEAST
AccessioniPrimary (citable) accession number: Q01926
Secondary accession number(s): D6W329
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally (PubMed:1551905 and PubMed:8252639) identified in genetic screens for bona fide RNA splicing factors, but it has later been shown that not the MRS2 protein per se but certain magnesium concentrations are essential for group II intron splicing.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.