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Q01926

- MRS2_YEAST

UniProt

Q01926 - MRS2_YEAST

Protein

Magnesium transporter MRS2, mitochondrial

Gene

MRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.4 Publications

    GO - Molecular functioni

    1. magnesium ion transmembrane transporter activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. mitochondrial magnesium ion transport Source: SGD

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33809-MONOMER.

    Protein family/group databases

    TCDBi1.A.35.5.1. the cora metal ion transporter (mit) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Magnesium transporter MRS2, mitochondrial
    Alternative name(s):
    RNA-splicing protein MRS2
    Gene namesi
    Name:MRS2
    Ordered Locus Names:YOR334W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR334w.
    SGDiS000005861. MRS2.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232MitochondrionSequence AnalysisAdd
    BLAST
    Chaini33 – 470438Magnesium transporter MRS2, mitochondrialPRO_0000021753Add
    BLAST

    Proteomic databases

    MaxQBiQ01926.
    PaxDbiQ01926.

    Expressioni

    Gene expression databases

    GenevestigatoriQ01926.

    Interactioni

    Subunit structurei

    Forms homooligomers. Interacts with MFM1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MFM1Q027832EBI-11283,EBI-33672

    Protein-protein interaction databases

    BioGridi34717. 59 interactions.
    DIPiDIP-5325N.
    IntActiQ01926. 1 interaction.
    MINTiMINT-553075.
    STRINGi4932.YOR334W.

    Structurei

    Secondary structure

    1
    470
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi59 – 624
    Beta strandi63 – 697
    Beta strandi75 – 839
    Helixi84 – 907
    Helixi95 – 1006
    Beta strandi111 – 1144
    Beta strandi119 – 1235
    Beta strandi126 – 1305
    Beta strandi135 – 1384
    Helixi143 – 16119
    Helixi170 – 20637
    Helixi210 – 24132
    Helixi244 – 2496
    Helixi263 – 30341

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RKGX-ray1.28A48-308[»]
    ProteinModelPortaliQ01926.
    SMRiQ01926. Positions 48-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 314282Mitochondrial matrixSequence AnalysisAdd
    BLAST
    Topological domaini334 – 34411Mitochondrial intermembraneSequence AnalysisAdd
    BLAST
    Topological domaini362 – 470109Mitochondrial matrixSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei315 – 33319HelicalSequence AnalysisAdd
    BLAST
    Transmembranei345 – 36117HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG284534.
    GeneTreeiENSGT00390000009988.
    HOGENOMiHOG000248323.
    KOiK16075.
    OMAiCDEYVQQ.
    OrthoDBiEOG7SXWCP.

    Family and domain databases

    InterProiIPR026573. MRS2/LPE10.
    [Graphical view]
    PANTHERiPTHR13890. PTHR13890. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01926-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRRLLVRSI SCFQPLSRIT FGRPNTPFLR KYADTSTAAN TNSTILRKQL    50
    LSLKPISASD SLFISCTVFN SKGNIISMSE KFPKWSFLTE HSLFPRDLRK 100
    IDNSSIDIIP TIMCKPNCIV INLLHIKALI ERDKVYVFDT TNPSAAAKLS 150
    VLMYDLESKL SSTKNNSQFY EHRALESIFI NVMSALETDF KLHSQICIQI 200
    LNDLENEVNR LKLRHLLIKS KDLTLFYQKT LLIRDLLDEL LENDDDLANM 250
    YLTVKKSPKD NFSDLEMLIE TYYTQCDEYV QQSESLIQDI KSTEEIVNII 300
    LDANRNSLML LELKVTIYTL GFTVASVLPA FYGMNLKNFI EESEWGFTSV 350
    AVFSIVSALY ITKKNFNSLR SVTKMTMYPN SPANSSVYPK TSASIALTNK 400
    LKRRRKWWKS TKQRLGVLLY GSSYTNKANL SNNKINKGFS KVKKFNMEND 450
    IKNKQNRDMI WKWLIEDKKN 470
    Length:470
    Mass (Da):54,203
    Last modified:October 1, 1996 - v2
    Checksum:iEFEBA11765AB8B31
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti371 – 3711S → F in AAA34795. (PubMed:1551905)Curated
    Sequence conflicti371 – 3711S → F(PubMed:8252639)Curated
    Sequence conflicti448 – 4481E → D in AAA34795. (PubMed:1551905)Curated
    Sequence conflicti448 – 4481E → D(PubMed:8252639)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82916 Genomic DNA. Translation: AAA34795.1.
    Z49821 Genomic DNA. Translation: CAA89979.1.
    Z75241 Genomic DNA. Translation: CAA99656.1.
    BK006948 Genomic DNA. Translation: DAA11095.1.
    PIRiS62064.
    RefSeqiNP_014979.1. NM_001183754.1.

    Genome annotation databases

    EnsemblFungiiYOR334W; YOR334W; YOR334W.
    GeneIDi854511.
    KEGGisce:YOR334W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82916 Genomic DNA. Translation: AAA34795.1 .
    Z49821 Genomic DNA. Translation: CAA89979.1 .
    Z75241 Genomic DNA. Translation: CAA99656.1 .
    BK006948 Genomic DNA. Translation: DAA11095.1 .
    PIRi S62064.
    RefSeqi NP_014979.1. NM_001183754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RKG X-ray 1.28 A 48-308 [» ]
    ProteinModelPortali Q01926.
    SMRi Q01926. Positions 48-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34717. 59 interactions.
    DIPi DIP-5325N.
    IntActi Q01926. 1 interaction.
    MINTi MINT-553075.
    STRINGi 4932.YOR334W.

    Protein family/group databases

    TCDBi 1.A.35.5.1. the cora metal ion transporter (mit) family.

    Proteomic databases

    MaxQBi Q01926.
    PaxDbi Q01926.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR334W ; YOR334W ; YOR334W .
    GeneIDi 854511.
    KEGGi sce:YOR334W.

    Organism-specific databases

    CYGDi YOR334w.
    SGDi S000005861. MRS2.

    Phylogenomic databases

    eggNOGi NOG284534.
    GeneTreei ENSGT00390000009988.
    HOGENOMi HOG000248323.
    KOi K16075.
    OMAi CDEYVQQ.
    OrthoDBi EOG7SXWCP.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33809-MONOMER.

    Miscellaneous databases

    NextBioi 976867.

    Gene expression databases

    Genevestigatori Q01926.

    Family and domain databases

    InterProi IPR026573. MRS2/LPE10.
    [Graphical view ]
    PANTHERi PTHR13890. PTHR13890. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo."
      Wiesenberger G., Waldherr M., Schweyen R.J.
      J. Biol. Chem. 267:6963-6969(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 44774 / DBY747.
    2. "A multitude of suppressors of group II intron-splicing defects in yeast."
      Waldherr M., Ragnini A., Jank B., Teply R., Wiesenberger G., Schweyen R.J.
      Curr. Genet. 24:301-306(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I."
      Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H.
      Yeast 12:999-1004(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo."
      Gregan J., Kolisek M., Schweyen R.J.
      Genes Dev. 15:2229-2237(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Insertion of bitopic membrane proteins into the inner membrane of mitochondria involves an export step from the matrix."
      Baumann F., Neupert W., Herrmann J.M.
      J. Biol. Chem. 277:21405-21413(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
    8. "Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria."
      Kolisek M., Zsurka G., Samaj J., Weghuber J., Schweyen R.J., Schweigel M.
      EMBO J. 22:1235-1244(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Mrs2p forms a high conductance Mg2+ selective channel in mitochondria."
      Schindl R., Weghuber J., Romanin C., Schweyen R.J.
      Biophys. J. 93:3872-3883(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+ channel."
      Sponder G., Svidova S., Schindl R., Wieser S., Schweyen R.J., Romanin C., Froschauer E.M., Weghuber J.
      FEBS J. 277:3514-3525(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MFM1.

    Entry informationi

    Entry nameiMRS2_YEAST
    AccessioniPrimary (citable) accession number: Q01926
    Secondary accession number(s): D6W329
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 768 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was originally (PubMed:1551905 and PubMed:8252639) identified in genetic screens for bona fide RNA splicing factors, but it has later been shown that not the MRS2 protein per se but certain magnesium concentrations are essential for group II intron splicing.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3