Q01926 (MRS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Magnesium transporter MRS2, mitochondrial

Alternative name(s):
RNA-splicing protein MRS2

Gene names

Name:	MRS2
Ordered Locus Names:	YOR334W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes. Ref.6 Ref.8 Ref.11 Ref.12
Subunit structure	Forms homooligomers. Interacts with MFM1. Ref.12
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Ref.9.
Miscellaneous	Present with 768 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family. [View classification]
Caution	Was originally (Ref.1 and Ref.2) identified in genetic screens for bona fide RNA splicing factors, but it has later been shown that not the MRS2 protein per se but certain magnesium concentrations are essential for group II intron splicing (Ref.6).

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Ligand	Magnesium
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial magnesium ion transport Inferred from direct assay Ref.8. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial inner membrane Inferred from direct assay PubMed 10400670. Source: SGD
Molecular_function	magnesium ion transmembrane transporter activity Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 32

32

Mitochondrion Potential

Chain

33 – 470

438

Magnesium transporter MRS2, mitochondrial

PRO_0000021753

Regions

Topological domain

33 – 314

282

Mitochondrial matrix Potential

Transmembrane

315 – 333

19

Helical; Potential

Topological domain

334 – 344

11

Mitochondrial intermembrane Potential

Transmembrane

345 – 361

17

Helical; Potential

Topological domain

362 – 470

109

Mitochondrial matrix Potential

Experimental info

Sequence conflict

371

1

S → F in AAA34795. Ref.1

Sequence conflict

371

1

S → F Ref.2

Sequence conflict

448

1

E → D in AAA34795. Ref.1

Sequence conflict

448

1

E → D Ref.2

Secondary structure

1

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470

Helix Strand Turn

Details...

Helix

59 – 62

4

Beta strand

63 – 69

7

Beta strand

75 – 83

9

Helix

84 – 90

7

Helix

95 – 100

6

Beta strand

111 – 114

4

Beta strand

119 – 123

5

Beta strand

126 – 130

5

Beta strand

135 – 138

4

Helix

143 – 161

19

Helix

170 – 206

37

Helix

210 – 241

32

Helix

244 – 249

6

Helix

263 – 303

41

Sequences

	Sequence		Length	Mass (Da)
	Q01926 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: EFEBA11765AB8B31	FASTA	470	54,203
	10 20 30 40 50 60 MNRRLLVRSI SCFQPLSRIT FGRPNTPFLR KYADTSTAAN TNSTILRKQL LSLKPISASD 70 80 90 100 110 120 SLFISCTVFN SKGNIISMSE KFPKWSFLTE HSLFPRDLRK IDNSSIDIIP TIMCKPNCIV 130 140 150 160 170 180 INLLHIKALI ERDKVYVFDT TNPSAAAKLS VLMYDLESKL SSTKNNSQFY EHRALESIFI 190 200 210 220 230 240 NVMSALETDF KLHSQICIQI LNDLENEVNR LKLRHLLIKS KDLTLFYQKT LLIRDLLDEL 250 260 270 280 290 300 LENDDDLANM YLTVKKSPKD NFSDLEMLIE TYYTQCDEYV QQSESLIQDI KSTEEIVNII 310 320 330 340 350 360 LDANRNSLML LELKVTIYTL GFTVASVLPA FYGMNLKNFI EESEWGFTSV AVFSIVSALY 370 380 390 400 410 420 ITKKNFNSLR SVTKMTMYPN SPANSSVYPK TSASIALTNK LKRRRKWWKS TKQRLGVLLY 430 440 450 460 470 GSSYTNKANL SNNKINKGFS KVKKFNMEND IKNKQNRDMI WKWLIEDKKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo." Wiesenberger G., Waldherr M., Schweyen R.J. J. Biol. Chem. 267:6963-6969(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 44774 / DBY747.
[2]	"A multitude of suppressors of group II intron-splicing defects in yeast." Waldherr M., Ragnini A., Jank B., Teply R., Wiesenberger G., Schweyen R.J. Curr. Genet. 24:301-306(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I." Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H. Yeast 12:999-1004(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo." Gregan J., Kolisek M., Schweyen R.J. Genes Dev. 15:2229-2237(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Insertion of bitopic membrane proteins into the inner membrane of mitochondria involves an export step from the matrix." Baumann F., Neupert W., Herrmann J.M. J. Biol. Chem. 277:21405-21413(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
[8]	"Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria." Kolisek M., Zsurka G., Samaj J., Weghuber J., Schweyen R.J., Schweigel M. EMBO J. 22:1235-1244(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Mrs2p forms a high conductance Mg2+ selective channel in mitochondria." Schindl R., Weghuber J., Romanin C., Schweyen R.J. Biophys. J. 93:3872-3883(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+ channel." Sponder G., Svidova S., Schindl R., Wieser S., Schweyen R.J., Romanin C., Froschauer E.M., Weghuber J. FEBS J. 277:3514-3525(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MFM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M82916 Genomic DNA. Translation: AAA34795.1.
Z49821 Genomic DNA. Translation: CAA89979.1.
Z75241 Genomic DNA. Translation: CAA99656.1.
BK006948 Genomic DNA. Translation: DAA11095.1.

PIR

S62064.

RefSeq

NP_014979.1. NM_001183754.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RKG	X-ray	1.28	A	48-308	[»]

ProteinModelPortal

Q01926.

SMR

Q01926. Positions 48-305.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5325N.

MINT

MINT-553075.

STRING

4932.YOR334W.

Protein family/group databases

TCDB

1.A.35.5.1. CorA metal ion transporter (MIT) family.

Proteomic databases

PaxDb

Q01926.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOR334W; YOR334W; YOR334W.

GeneID

854511.

KEGG

sce:YOR334W.

Organism-specific databases

CYGD

YOR334w.

SGD

S000005861. MRS2.

Phylogenomic databases

eggNOG

NOG284534.

GeneTree

ENSGT00390000009988.

HOGENOM

HOG000248323.

KO

K16075.

OMA

LMYDLES.

OrthoDB

EOG432474.

Gene expression databases

Genevestigator

Q01926.

GermOnline

YOR334W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR026573. MRS2/LPE10.
[Graphical view]

PANTHER

PTHR13890. PTHR13890. 1 hit.

ProtoNet

Search...

Other

NextBio

976867.

Entry information

Entry name

MRS2_YEAST

Accession

Primary (citable) accession number: Q01926
Secondary accession number(s): D6W329

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program