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Q01926

- MRS2_YEAST

UniProt

Q01926 - MRS2_YEAST

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Protein

Magnesium transporter MRS2, mitochondrial

Gene
MRS2, YOR334W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.4 Publications

GO - Molecular functioni

  1. magnesium ion transmembrane transporter activity Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. mitochondrial magnesium ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciYEAST:G3O-33809-MONOMER.

Protein family/group databases

TCDBi1.A.35.5.1. the cora metal ion transporter (mit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium transporter MRS2, mitochondrial
Alternative name(s):
RNA-splicing protein MRS2
Gene namesi
Name:MRS2
Ordered Locus Names:YOR334W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR334w.
SGDiS000005861. MRS2.

Subcellular locationi

Mitochondrion inner membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 314282Mitochondrial matrix Reviewed predictionAdd
BLAST
Transmembranei315 – 33319Helical; Reviewed predictionAdd
BLAST
Topological domaini334 – 34411Mitochondrial intermembrane Reviewed predictionAdd
BLAST
Transmembranei345 – 36117Helical; Reviewed predictionAdd
BLAST
Topological domaini362 – 470109Mitochondrial matrix Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232Mitochondrion Reviewed predictionAdd
BLAST
Chaini33 – 470438Magnesium transporter MRS2, mitochondrialPRO_0000021753Add
BLAST

Proteomic databases

MaxQBiQ01926.
PaxDbiQ01926.

Expressioni

Gene expression databases

GenevestigatoriQ01926.

Interactioni

Subunit structurei

Forms homooligomers. Interacts with MFM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MFM1Q027832EBI-11283,EBI-33672

Protein-protein interaction databases

BioGridi34717. 59 interactions.
DIPiDIP-5325N.
IntActiQ01926. 1 interaction.
MINTiMINT-553075.
STRINGi4932.YOR334W.

Structurei

Secondary structure

1
470
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi59 – 624
Beta strandi63 – 697
Beta strandi75 – 839
Helixi84 – 907
Helixi95 – 1006
Beta strandi111 – 1144
Beta strandi119 – 1235
Beta strandi126 – 1305
Beta strandi135 – 1384
Helixi143 – 16119
Helixi170 – 20637
Helixi210 – 24132
Helixi244 – 2496
Helixi263 – 30341

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RKGX-ray1.28A48-308[»]
ProteinModelPortaliQ01926.
SMRiQ01926. Positions 48-305.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG284534.
GeneTreeiENSGT00390000009988.
HOGENOMiHOG000248323.
KOiK16075.
OMAiCDEYVQQ.
OrthoDBiEOG7SXWCP.

Family and domain databases

InterProiIPR026573. MRS2/LPE10.
[Graphical view]
PANTHERiPTHR13890. PTHR13890. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01926-1 [UniParc]FASTAAdd to Basket

« Hide

MNRRLLVRSI SCFQPLSRIT FGRPNTPFLR KYADTSTAAN TNSTILRKQL    50
LSLKPISASD SLFISCTVFN SKGNIISMSE KFPKWSFLTE HSLFPRDLRK 100
IDNSSIDIIP TIMCKPNCIV INLLHIKALI ERDKVYVFDT TNPSAAAKLS 150
VLMYDLESKL SSTKNNSQFY EHRALESIFI NVMSALETDF KLHSQICIQI 200
LNDLENEVNR LKLRHLLIKS KDLTLFYQKT LLIRDLLDEL LENDDDLANM 250
YLTVKKSPKD NFSDLEMLIE TYYTQCDEYV QQSESLIQDI KSTEEIVNII 300
LDANRNSLML LELKVTIYTL GFTVASVLPA FYGMNLKNFI EESEWGFTSV 350
AVFSIVSALY ITKKNFNSLR SVTKMTMYPN SPANSSVYPK TSASIALTNK 400
LKRRRKWWKS TKQRLGVLLY GSSYTNKANL SNNKINKGFS KVKKFNMEND 450
IKNKQNRDMI WKWLIEDKKN 470
Length:470
Mass (Da):54,203
Last modified:October 1, 1996 - v2
Checksum:iEFEBA11765AB8B31
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti371 – 3711S → F in AAA34795. 1 Publication
Sequence conflicti371 – 3711S → F1 Publication
Sequence conflicti448 – 4481E → D in AAA34795. 1 Publication
Sequence conflicti448 – 4481E → D1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82916 Genomic DNA. Translation: AAA34795.1.
Z49821 Genomic DNA. Translation: CAA89979.1.
Z75241 Genomic DNA. Translation: CAA99656.1.
BK006948 Genomic DNA. Translation: DAA11095.1.
PIRiS62064.
RefSeqiNP_014979.1. NM_001183754.1.

Genome annotation databases

EnsemblFungiiYOR334W; YOR334W; YOR334W.
GeneIDi854511.
KEGGisce:YOR334W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82916 Genomic DNA. Translation: AAA34795.1 .
Z49821 Genomic DNA. Translation: CAA89979.1 .
Z75241 Genomic DNA. Translation: CAA99656.1 .
BK006948 Genomic DNA. Translation: DAA11095.1 .
PIRi S62064.
RefSeqi NP_014979.1. NM_001183754.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3RKG X-ray 1.28 A 48-308 [» ]
ProteinModelPortali Q01926.
SMRi Q01926. Positions 48-305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34717. 59 interactions.
DIPi DIP-5325N.
IntActi Q01926. 1 interaction.
MINTi MINT-553075.
STRINGi 4932.YOR334W.

Protein family/group databases

TCDBi 1.A.35.5.1. the cora metal ion transporter (mit) family.

Proteomic databases

MaxQBi Q01926.
PaxDbi Q01926.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR334W ; YOR334W ; YOR334W .
GeneIDi 854511.
KEGGi sce:YOR334W.

Organism-specific databases

CYGDi YOR334w.
SGDi S000005861. MRS2.

Phylogenomic databases

eggNOGi NOG284534.
GeneTreei ENSGT00390000009988.
HOGENOMi HOG000248323.
KOi K16075.
OMAi CDEYVQQ.
OrthoDBi EOG7SXWCP.

Enzyme and pathway databases

BioCyci YEAST:G3O-33809-MONOMER.

Miscellaneous databases

NextBioi 976867.

Gene expression databases

Genevestigatori Q01926.

Family and domain databases

InterProi IPR026573. MRS2/LPE10.
[Graphical view ]
PANTHERi PTHR13890. PTHR13890. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo."
    Wiesenberger G., Waldherr M., Schweyen R.J.
    J. Biol. Chem. 267:6963-6969(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 44774 / DBY747.
  2. "A multitude of suppressors of group II intron-splicing defects in yeast."
    Waldherr M., Ragnini A., Jank B., Teply R., Wiesenberger G., Schweyen R.J.
    Curr. Genet. 24:301-306(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I."
    Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H.
    Yeast 12:999-1004(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo."
    Gregan J., Kolisek M., Schweyen R.J.
    Genes Dev. 15:2229-2237(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Insertion of bitopic membrane proteins into the inner membrane of mitochondria involves an export step from the matrix."
    Baumann F., Neupert W., Herrmann J.M.
    J. Biol. Chem. 277:21405-21413(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  8. "Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria."
    Kolisek M., Zsurka G., Samaj J., Weghuber J., Schweyen R.J., Schweigel M.
    EMBO J. 22:1235-1244(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Mrs2p forms a high conductance Mg2+ selective channel in mitochondria."
    Schindl R., Weghuber J., Romanin C., Schweyen R.J.
    Biophys. J. 93:3872-3883(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+ channel."
    Sponder G., Svidova S., Schindl R., Wieser S., Schweyen R.J., Romanin C., Froschauer E.M., Weghuber J.
    FEBS J. 277:3514-3525(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MFM1.

Entry informationi

Entry nameiMRS2_YEAST
AccessioniPrimary (citable) accession number: Q01926
Secondary accession number(s): D6W329
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.

Caution

Was originally (1 Publication and 1 Publication) identified in genetic screens for bona fide RNA splicing factors, but it has later been shown that not the MRS2 protein per se but certain magnesium concentrations are essential for group II intron splicing (1 Publication).

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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