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Protein

Transcription factor Spi-B

Gene

SPIB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi169 – 252ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: GO_Central
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • regulation of transcription by RNA polymerase II Source: ProtInc

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SignaLinkiQ01892
SIGNORiQ01892

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Spi-B
Gene namesi
Name:SPIB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000269404.6
HGNCiHGNC:11242 SPIB
MIMi606802 gene
neXtProtiNX_Q01892

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi144S → A: Reduces interaction with IRF4 and transcriptional activation. 1 Publication1
Mutagenesisi242K → G: Abrogates DNA-binding. 1 Publication1

Organism-specific databases

DisGeNETi6689
MalaCardsiSPIB
OpenTargetsiENSG00000269404
Orphaneti186 Primary biliary cirrhosis
PharmGKBiPA36072

Polymorphism and mutation databases

BioMutaiSPIB
DMDMi548971

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041361 – 262Transcription factor Spi-BAdd BLAST262

Proteomic databases

MaxQBiQ01892
PaxDbiQ01892
PeptideAtlasiQ01892
PRIDEiQ01892

PTM databases

iPTMnetiQ01892

Expressioni

Tissue specificityi

Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.2 Publications

Gene expression databases

BgeeiENSG00000269404
CleanExiHS_SPIB
ExpressionAtlasiQ01892 baseline and differential
GenevisibleiQ01892 HS

Organism-specific databases

HPAiHPA018523

Interactioni

Subunit structurei

Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP.2 Publications

Protein-protein interaction databases

BioGridi112567, 16 interactors
IntActiQ01892, 3 interactors
STRINGi9606.ENSP00000471921

Structurei

3D structure databases

ProteinModelPortaliQ01892
SMRiQ01892
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 31TAD1 (Acidic)Add BLAST31
Regioni41 – 61TAD2Add BLAST21

Domaini

The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes.1 Publication

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3805 Eukaryota
ENOG410XSXU LUCA
GeneTreeiENSGT00390000015212
HOGENOMiHOG000095520
HOVERGENiHBG002474
InParanoidiQ01892
KOiK09439
OrthoDBiEOG091G0DTY
PhylomeDBiQ01892
TreeFamiTF352494

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q01892-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLALEAAQLD GPHFSCLYPD GVFYDLDSCK HSSYPDSEGA PDSLWDWTVA
60 70 80 90 100
PPVPATPYEA FDPAAAAFSH PQAAQLCYEP PTYSPAGNLE LAPSLEAPGP
110 120 130 140 150
GLPAYPTENF ASQTLVPPAY APYPSPVLSE EEDLPLDSPA LEVSDSESDE
160 170 180 190 200
ALVAGPEGKG SEAGTRKKLR LYQFLLGLLT RGDMRECVWW VEPGAGVFQF
210 220 230 240 250
SSKHKELLAR RWGQQKGNRK RMTYQKLARA LRNYAKTGEI RKVKRKLTYQ
260
FDSALLPAVR RA
Length:262
Mass (Da):28,819
Last modified:June 1, 1994 - v1
Checksum:iA6C21DA1BBF61B6F
GO
Isoform 2 (identifier: Q01892-2) [UniParc]FASTAAdd to basket
Also known as: DeltaSpi-B

The sequence of this isoform differs from the canonical sequence as follows:
     163-177: AGTRKKLRLYQFLLG → GLARSCACTSSCWGY
     178-262: Missing.

Show »
Length:177
Mass (Da):18,585
Checksum:iB138A7F7706542E7
GO
Isoform 3 (identifier: Q01892-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     92-113: APSLEAPGPGLPAYPTENFASQ → MASSMTWTAASIPATLIQRGLL

Show »
Length:171
Mass (Da):19,137
Checksum:i4F0E45F5CABCB71E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061150104A → P1 PublicationCorresponds to variant dbSNP:rs11546996Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0451241 – 91Missing in isoform 3. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_04512592 – 113APSLE…NFASQ → MASSMTWTAASIPATLIQRG LL in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_001479163 – 177AGTRK…QFLLG → GLARSCACTSSCWGY in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_001480178 – 262Missing in isoform 2. 1 PublicationAdd BLAST85

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66079 mRNA Translation: CAA46878.1
X96998 mRNA Translation: CAA65726.1
AK292644 mRNA Translation: BAF85333.1
AK300639 mRNA Translation: BAG62328.1
AC020909 Genomic DNA No translation available.
CH471135 Genomic DNA Translation: EAW71858.1
BC007921 mRNA Translation: AAH07921.1
CCDSiCCDS33080.1 [Q01892-1]
CCDS58674.1 [Q01892-3]
CCDS59412.1 [Q01892-2]
PIRiJC4839
S25655
RefSeqiNP_001230927.1, NM_001243998.1 [Q01892-3]
NP_001230928.1, NM_001243999.1 [Q01892-2]
NP_001230929.1, NM_001244000.1
NP_003112.2, NM_003121.4 [Q01892-1]
UniGeneiHs.437905

Genome annotation databases

EnsembliENST00000270632; ENSP00000270632; ENSG00000269404 [Q01892-2]
ENST00000439922; ENSP00000391877; ENSG00000269404 [Q01892-3]
ENST00000595883; ENSP00000471921; ENSG00000269404 [Q01892-1]
GeneIDi6689
KEGGihsa:6689
UCSCiuc002psd.4 human [Q01892-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSPIB_HUMAN
AccessioniPrimary (citable) accession number: Q01892
Secondary accession number(s): A8K9C9, B4DUG6, Q15359
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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