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Q01892

- SPIB_HUMAN

UniProt

Q01892 - SPIB_HUMAN

Protein

Transcription factor Spi-B

Gene

SPIB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi169 – 25284ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome

    GO - Biological processi

    1. cell differentiation Source: RefGenome
    2. regulation of transcription from RNA polymerase II promoter Source: RefGenome
    3. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    SignaLinkiQ01892.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor Spi-B
    Gene namesi
    Name:SPIB
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:11242. SPIB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi144 – 1441S → A: Reduces interaction with IRF4 and transcriptional activation. 1 Publication
    Mutagenesisi242 – 2421K → G: Abrogates DNA-binding. 1 Publication

    Organism-specific databases

    Orphaneti186. Primary biliary cirrhosis.
    PharmGKBiPA36072.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 262262Transcription factor Spi-BPRO_0000204136Add
    BLAST

    Proteomic databases

    MaxQBiQ01892.
    PaxDbiQ01892.
    PRIDEiQ01892.

    Expressioni

    Tissue specificityi

    Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.2 Publications

    Gene expression databases

    ArrayExpressiQ01892.
    BgeeiQ01892.
    CleanExiHS_SPIB.
    GenevestigatoriQ01892.

    Organism-specific databases

    HPAiHPA018523.

    Interactioni

    Subunit structurei

    Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP.2 Publications

    Protein-protein interaction databases

    BioGridi112567. 14 interactions.
    IntActiQ01892. 3 interactions.
    STRINGi9606.ENSP00000270632.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01892.
    SMRiQ01892. Positions 130-251.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3131TAD1 (Acidic)Add
    BLAST
    Regioni41 – 6121TAD2Add
    BLAST

    Domaini

    The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes.1 Publication

    Sequence similaritiesi

    Belongs to the ETS family.Curated
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG241814.
    HOGENOMiHOG000095520.
    HOVERGENiHBG002474.
    InParanoidiQ01892.
    KOiK09439.
    PhylomeDBiQ01892.
    TreeFamiTF352494.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    [Graphical view]
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    [Graphical view]
    PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q01892-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLALEAAQLD GPHFSCLYPD GVFYDLDSCK HSSYPDSEGA PDSLWDWTVA    50
    PPVPATPYEA FDPAAAAFSH PQAAQLCYEP PTYSPAGNLE LAPSLEAPGP 100
    GLPAYPTENF ASQTLVPPAY APYPSPVLSE EEDLPLDSPA LEVSDSESDE 150
    ALVAGPEGKG SEAGTRKKLR LYQFLLGLLT RGDMRECVWW VEPGAGVFQF 200
    SSKHKELLAR RWGQQKGNRK RMTYQKLARA LRNYAKTGEI RKVKRKLTYQ 250
    FDSALLPAVR RA 262
    Length:262
    Mass (Da):28,819
    Last modified:June 1, 1994 - v1
    Checksum:iA6C21DA1BBF61B6F
    GO
    Isoform 2 (identifier: Q01892-2) [UniParc]FASTAAdd to Basket

    Also known as: DeltaSpi-B

    The sequence of this isoform differs from the canonical sequence as follows:
         163-177: AGTRKKLRLYQFLLG → GLARSCACTSSCWGY
         178-262: Missing.

    Show »
    Length:177
    Mass (Da):18,585
    Checksum:iB138A7F7706542E7
    GO
    Isoform 3 (identifier: Q01892-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-91: Missing.
         92-113: APSLEAPGPGLPAYPTENFASQ → MASSMTWTAASIPATLIQRGLL

    Show »
    Length:171
    Mass (Da):19,137
    Checksum:i4F0E45F5CABCB71E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti104 – 1041A → P.1 Publication
    Corresponds to variant rs11546996 [ dbSNP | Ensembl ].
    VAR_061150

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9191Missing in isoform 3. 1 PublicationVSP_045124Add
    BLAST
    Alternative sequencei92 – 11322APSLE…NFASQ → MASSMTWTAASIPATLIQRG LL in isoform 3. 1 PublicationVSP_045125Add
    BLAST
    Alternative sequencei163 – 17715AGTRK…QFLLG → GLARSCACTSSCWGY in isoform 2. 1 PublicationVSP_001479Add
    BLAST
    Alternative sequencei178 – 26285Missing in isoform 2. 1 PublicationVSP_001480Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66079 mRNA. Translation: CAA46878.1.
    X96998 mRNA. Translation: CAA65726.1.
    AK292644 mRNA. Translation: BAF85333.1.
    AK300639 mRNA. Translation: BAG62328.1.
    AC020909 Genomic DNA. No translation available.
    CH471135 Genomic DNA. Translation: EAW71858.1.
    BC007921 mRNA. Translation: AAH07921.1.
    CCDSiCCDS33080.1. [Q01892-1]
    CCDS58674.1. [Q01892-3]
    CCDS59412.1. [Q01892-2]
    PIRiJC4839.
    S25655.
    RefSeqiNP_001230927.1. NM_001243998.1. [Q01892-3]
    NP_001230928.1. NM_001243999.1. [Q01892-2]
    NP_001230929.1. NM_001244000.1.
    NP_003112.2. NM_003121.4. [Q01892-1]
    UniGeneiHs.437905.

    Genome annotation databases

    GeneIDi6689.
    KEGGihsa:6689.
    UCSCiuc002psd.3. human. [Q01892-1]
    uc002pse.3. human. [Q01892-2]
    uc010ycc.2. human.

    Polymorphism databases

    DMDMi548971.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66079 mRNA. Translation: CAA46878.1 .
    X96998 mRNA. Translation: CAA65726.1 .
    AK292644 mRNA. Translation: BAF85333.1 .
    AK300639 mRNA. Translation: BAG62328.1 .
    AC020909 Genomic DNA. No translation available.
    CH471135 Genomic DNA. Translation: EAW71858.1 .
    BC007921 mRNA. Translation: AAH07921.1 .
    CCDSi CCDS33080.1. [Q01892-1 ]
    CCDS58674.1. [Q01892-3 ]
    CCDS59412.1. [Q01892-2 ]
    PIRi JC4839.
    S25655.
    RefSeqi NP_001230927.1. NM_001243998.1. [Q01892-3 ]
    NP_001230928.1. NM_001243999.1. [Q01892-2 ]
    NP_001230929.1. NM_001244000.1.
    NP_003112.2. NM_003121.4. [Q01892-1 ]
    UniGenei Hs.437905.

    3D structure databases

    ProteinModelPortali Q01892.
    SMRi Q01892. Positions 130-251.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112567. 14 interactions.
    IntActi Q01892. 3 interactions.
    STRINGi 9606.ENSP00000270632.

    Polymorphism databases

    DMDMi 548971.

    Proteomic databases

    MaxQBi Q01892.
    PaxDbi Q01892.
    PRIDEi Q01892.

    Protocols and materials databases

    DNASUi 6689.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6689.
    KEGGi hsa:6689.
    UCSCi uc002psd.3. human. [Q01892-1 ]
    uc002pse.3. human. [Q01892-2 ]
    uc010ycc.2. human.

    Organism-specific databases

    CTDi 6689.
    GeneCardsi GC19P050922.
    HGNCi HGNC:11242. SPIB.
    HPAi HPA018523.
    MIMi 606802. gene.
    neXtProti NX_Q01892.
    Orphaneti 186. Primary biliary cirrhosis.
    PharmGKBi PA36072.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG241814.
    HOGENOMi HOG000095520.
    HOVERGENi HBG002474.
    InParanoidi Q01892.
    KOi K09439.
    PhylomeDBi Q01892.
    TreeFami TF352494.

    Enzyme and pathway databases

    SignaLinki Q01892.

    Miscellaneous databases

    GeneWikii SPIB.
    GenomeRNAii 6689.
    NextBioi 26069.
    PROi Q01892.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q01892.
    Bgeei Q01892.
    CleanExi HS_SPIB.
    Genevestigatori Q01892.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    [Graphical view ]
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    [Graphical view ]
    PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1."
      Ray D., Bosselut R., Ghysdael J., Mattei M.-G., Tavitian A., Moreau-Gachelin F.
      Mol. Cell. Biol. 12:4297-4304(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    2. "An alternatively spliced isoform of the Spi-B transcription factor."
      Ray-Gallet D., Tavitian A., Moreau-Gachelin F.
      Biochem. Biophys. Res. Commun. 223:257-263(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT PRO-104.
      Tissue: Rectum and Thymus.
    4. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: B-cell.
    7. "SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1."
      Rao S., Matsumura A., Yoon J., Simon M.C.
      J. Biol. Chem. 274:11115-11124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH IRF4; JUN; SPIB; SPI1 AND TBP, DOMAINS PEST; TAD1 AND TAD2, MUTAGENESIS OF SER-144 AND LYS-242.
    8. "Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells."
      Nagy M., Chapuis B., Matthes T.
      Br. J. Haematol. 116:429-435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    9. "Interaction between the hematopoietic Ets transcription factor Spi-B and the coactivator CREB-binding protein associated with negative cross-talk with c-Myb."
      Yamamoto H., Kihara-Negishi F., Yamada T., Suzuki M., Nakano T., Oikawa T.
      Cell Growth Differ. 13:69-75(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CREBBP AND EP300.
    10. "The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development."
      Schotte R., Rissoan M.-C., Bendriss-Vermare N., Bridon J.-M., Duhen T., Weijer K., Briere F., Spits H.
      Blood 101:1015-1023(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    11. "The ETS transcription factor Spi-B is required for human plasmacytoid dendritic cell development."
      Schotte R., Nagasawa M., Weijer K., Spits H., Blom B.
      J. Exp. Med. 200:1503-1509(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSPIB_HUMAN
    AccessioniPrimary (citable) accession number: Q01892
    Secondary accession number(s): A8K9C9, B4DUG6, Q15359
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3