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Q01892 (SPIB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor Spi-B
Gene names
Name:SPIB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. Ref.1 Ref.7 Ref.10 Ref.11

Subunit structure

Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP. Ref.7 Ref.9

Subcellular location

Isoform 1: Nucleus Ref.10.

Isoform 2: Cytoplasm Ref.10.

Tissue specificity

Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver. Ref.8 Ref.10

Domain

The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes. Ref.7

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q01892-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q01892-2)

Also known as: DeltaSpi-B;

The sequence of this isoform differs from the canonical sequence as follows:
     163-177: AGTRKKLRLYQFLLG → GLARSCACTSSCWGY
     178-262: Missing.
Isoform 3 (identifier: Q01892-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     92-113: APSLEAPGPGLPAYPTENFASQ → MASSMTWTAASIPATLIQRGLL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Transcription factor Spi-B
PRO_0000204136

Regions

DNA binding169 – 25284ETS
Region1 – 3131TAD1 (Acidic)
Region41 – 6121TAD2

Natural variations

Alternative sequence1 – 9191Missing in isoform 3.
VSP_045124
Alternative sequence92 – 11322APSLE…NFASQ → MASSMTWTAASIPATLIQRG LL in isoform 3.
VSP_045125
Alternative sequence163 – 17715AGTRK…QFLLG → GLARSCACTSSCWGY in isoform 2.
VSP_001479
Alternative sequence178 – 26285Missing in isoform 2.
VSP_001480
Natural variant1041A → P. Ref.3
Corresponds to variant rs11546996 [ dbSNP | Ensembl ].
VAR_061150

Experimental info

Mutagenesis1441S → A: Reduces interaction with IRF4 and transcriptional activation. Ref.7
Mutagenesis2421K → G: Abrogates DNA-binding. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: A6C21DA1BBF61B6F

FASTA26228,819
        10         20         30         40         50         60 
MLALEAAQLD GPHFSCLYPD GVFYDLDSCK HSSYPDSEGA PDSLWDWTVA PPVPATPYEA 

        70         80         90        100        110        120 
FDPAAAAFSH PQAAQLCYEP PTYSPAGNLE LAPSLEAPGP GLPAYPTENF ASQTLVPPAY 

       130        140        150        160        170        180 
APYPSPVLSE EEDLPLDSPA LEVSDSESDE ALVAGPEGKG SEAGTRKKLR LYQFLLGLLT 

       190        200        210        220        230        240 
RGDMRECVWW VEPGAGVFQF SSKHKELLAR RWGQQKGNRK RMTYQKLARA LRNYAKTGEI 

       250        260 
RKVKRKLTYQ FDSALLPAVR RA 

« Hide

Isoform 2 (DeltaSpi-B) [UniParc].

Checksum: B138A7F7706542E7
Show »

FASTA17718,585
Isoform 3 [UniParc].

Checksum: 4F0E45F5CABCB71E
Show »

FASTA17119,137

References

« Hide 'large scale' references
[1]"Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1."
Ray D., Bosselut R., Ghysdael J., Mattei M.-G., Tavitian A., Moreau-Gachelin F.
Mol. Cell. Biol. 12:4297-4304(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]"An alternatively spliced isoform of the Spi-B transcription factor."
Ray-Gallet D., Tavitian A., Moreau-Gachelin F.
Biochem. Biophys. Res. Commun. 223:257-263(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT PRO-104.
Tissue: Rectum and Thymus.
[4]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: B-cell.
[7]"SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1."
Rao S., Matsumura A., Yoon J., Simon M.C.
J. Biol. Chem. 274:11115-11124(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH IRF4; JUN; SPIB; SPI1 AND TBP, DOMAINS PEST; TAD1 AND TAD2, MUTAGENESIS OF SER-144 AND LYS-242.
[8]"Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells."
Nagy M., Chapuis B., Matthes T.
Br. J. Haematol. 116:429-435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"Interaction between the hematopoietic Ets transcription factor Spi-B and the coactivator CREB-binding protein associated with negative cross-talk with c-Myb."
Yamamoto H., Kihara-Negishi F., Yamada T., Suzuki M., Nakano T., Oikawa T.
Cell Growth Differ. 13:69-75(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CREBBP AND EP300.
[10]"The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development."
Schotte R., Rissoan M.-C., Bendriss-Vermare N., Bridon J.-M., Duhen T., Weijer K., Briere F., Spits H.
Blood 101:1015-1023(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[11]"The ETS transcription factor Spi-B is required for human plasmacytoid dendritic cell development."
Schotte R., Nagasawa M., Weijer K., Spits H., Blom B.
J. Exp. Med. 200:1503-1509(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X66079 mRNA. Translation: CAA46878.1.
X96998 mRNA. Translation: CAA65726.1.
AK292644 mRNA. Translation: BAF85333.1.
AK300639 mRNA. Translation: BAG62328.1.
AC020909 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW71858.1.
BC007921 mRNA. Translation: AAH07921.1.
PIRJC4839.
S25655.
RefSeqNP_001230927.1. NM_001243998.1.
NP_001230928.1. NM_001243999.1.
NP_001230929.1. NM_001244000.1.
NP_003112.2. NM_003121.4.
UniGeneHs.437905.

3D structure databases

ProteinModelPortalQ01892.
SMRQ01892. Positions 130-251.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112567. 14 interactions.
IntActQ01892. 3 interactions.
STRING9606.ENSP00000270632.

Polymorphism databases

DMDM548971.

Proteomic databases

PaxDbQ01892.
PRIDEQ01892.

Protocols and materials databases

DNASU6689.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000270632; ENSP00000270632; ENSG00000269404. [Q01892-2]
ENST00000439922; ENSP00000391877; ENSG00000269404. [Q01892-3]
ENST00000595883; ENSP00000471921; ENSG00000269404. [Q01892-1]
GeneID6689.
KEGGhsa:6689.
UCSCuc002psd.3. human. [Q01892-1]
uc002pse.3. human. [Q01892-2]
uc010ycc.2. human.

Organism-specific databases

CTD6689.
GeneCardsGC19P050922.
HGNCHGNC:11242. SPIB.
HPAHPA018523.
MIM606802. gene.
neXtProtNX_Q01892.
Orphanet186. Primary biliary cirrhosis.
PharmGKBPA36072.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG241814.
HOGENOMHOG000095520.
HOVERGENHBG002474.
InParanoidQ01892.
KOK09439.
PhylomeDBQ01892.
TreeFamTF352494.

Enzyme and pathway databases

SignaLinkQ01892.

Gene expression databases

ArrayExpressQ01892.
BgeeQ01892.
CleanExHS_SPIB.
GenevestigatorQ01892.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00178. Ets. 1 hit.
[Graphical view]
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
[Graphical view]
PROSITEPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSPIB.
GenomeRNAi6689.
NextBio26069.
PROQ01892.
SOURCESearch...

Entry information

Entry nameSPIB_HUMAN
AccessionPrimary (citable) accession number: Q01892
Secondary accession number(s): A8K9C9, B4DUG6, Q15359
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM