Q01892 (SPIB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor Spi-B

Gene names

Name:

SPIB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. Ref.1 Ref.7 Ref.10 Ref.11
Subunit structure	Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP. Ref.7 Ref.9
Subcellular location	Isoform 1: Nucleus Ref.10. Isoform 2: Cytoplasm Ref.10.
Tissue specificity	Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver. Ref.8 Ref.10
Domain	The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes. Ref.7
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Traceable author statement Ref.2. Source: UniProtKB microtubule cytoskeleton Inferred from direct assay. Source: HPA nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q01892-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q01892-2)

Also known as: DeltaSpi-B;

The sequence of this isoform differs from the canonical sequence as follows:
     163-177: AGTRKKLRLYQFLLG → GLARSCACTSSCWGY
     178-262: Missing.

Isoform 3 (identifier: Q01892-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     92-113: APSLEAPGPGLPAYPTENFASQ → MASSMTWTAASIPATLIQRGLL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Transcription factor Spi-B		PRO_0000204136
Regions
	DNA binding	169 – 252	84	ETS
	Region	1 – 31	31	TAD1 (Acidic)
	Region	41 – 61	21	TAD2
Natural variations
	Alternative sequence	1 – 91	91	Missing in isoform 3.		VSP_045124
	Alternative sequence	92 – 113	22	APSLE…NFASQ → MASSMTWTAASIPATLIQRG LL in isoform 3.		VSP_045125
	Alternative sequence	163 – 177	15	AGTRK…QFLLG → GLARSCACTSSCWGY in isoform 2.		VSP_001479
	Alternative sequence	178 – 262	85	Missing in isoform 2.		VSP_001480
	Natural variant	104	1	A → P. Ref.3 Corresponds to variant rs11546996 [ dbSNP | Ensembl ].		VAR_061150
Experimental info
	Mutagenesis	144	1	S → A: Reduces interaction with IRF4 and transcriptional activation. Ref.7
	Mutagenesis	242	1	K → G: Abrogates DNA-binding. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: A6C21DA1BBF61B6F	FASTA	262	28,819
	10 20 30 40 50 60 MLALEAAQLD GPHFSCLYPD GVFYDLDSCK HSSYPDSEGA PDSLWDWTVA PPVPATPYEA 70 80 90 100 110 120 FDPAAAAFSH PQAAQLCYEP PTYSPAGNLE LAPSLEAPGP GLPAYPTENF ASQTLVPPAY 130 140 150 160 170 180 APYPSPVLSE EEDLPLDSPA LEVSDSESDE ALVAGPEGKG SEAGTRKKLR LYQFLLGLLT 190 200 210 220 230 240 RGDMRECVWW VEPGAGVFQF SSKHKELLAR RWGQQKGNRK RMTYQKLARA LRNYAKTGEI 250 260 RKVKRKLTYQ FDSALLPAVR RA
	Isoform 2 (DeltaSpi-B) [UniParc]. Checksum: B138A7F7706542E7	FASTA	177	18,585
	10 20 30 40 50 60 MLALEAAQLD GPHFSCLYPD GVFYDLDSCK HSSYPDSEGA PDSLWDWTVA PPVPATPYEA 70 80 90 100 110 120 FDPAAAAFSH PQAAQLCYEP PTYSPAGNLE LAPSLEAPGP GLPAYPTENF ASQTLVPPAY 130 140 150 160 170 APYPSPVLSE EEDLPLDSPA LEVSDSESDE ALVAGPEGKG SEGLARSCAC TSSCWGY
	Isoform 3 [UniParc]. Checksum: 4F0E45F5CABCB71E	FASTA	171	19,137
	10 20 30 40 50 60 MASSMTWTAA SIPATLIQRG LLTLVPPAYA PYPSPVLSEE EDLPLDSPAL EVSDSESDEA 70 80 90 100 110 120 LVAGPEGKGS EAGTRKKLRL YQFLLGLLTR GDMRECVWWV EPGAGVFQFS SKHKELLARR 130 140 150 160 170 WGQQKGNRKR MTYQKLARAL RNYAKTGEIR KVKRKLTYQF DSALLPAVRR A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1." Ray D., Bosselut R., Ghysdael J., Mattei M.-G., Tavitian A., Moreau-Gachelin F. Mol. Cell. Biol. 12:4297-4304(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]	"An alternatively spliced isoform of the Spi-B transcription factor." Ray-Gallet D., Tavitian A., Moreau-Gachelin F. Biochem. Biophys. Res. Commun. 223:257-263(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT PRO-104. Tissue: Rectum and Thymus.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: B-cell.
[7]	"SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1." Rao S., Matsumura A., Yoon J., Simon M.C. J. Biol. Chem. 274:11115-11124(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH IRF4; JUN; SPIB; SPI1 AND TBP, DOMAINS PEST; TAD1 AND TAD2, MUTAGENESIS OF SER-144 AND LYS-242.
[8]	"Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells." Nagy M., Chapuis B., Matthes T. Br. J. Haematol. 116:429-435(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Interaction between the hematopoietic Ets transcription factor Spi-B and the coactivator CREB-binding protein associated with negative cross-talk with c-Myb." Yamamoto H., Kihara-Negishi F., Yamada T., Suzuki M., Nakano T., Oikawa T. Cell Growth Differ. 13:69-75(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CREBBP AND EP300.
[10]	"The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development." Schotte R., Rissoan M.-C., Bendriss-Vermare N., Bridon J.-M., Duhen T., Weijer K., Briere F., Spits H. Blood 101:1015-1023(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[11]	"The ETS transcription factor Spi-B is required for human plasmacytoid dendritic cell development." Schotte R., Nagasawa M., Weijer K., Spits H., Blom B. J. Exp. Med. 200:1503-1509(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66079 mRNA. Translation: CAA46878.1. X96998 mRNA. Translation: CAA65726.1. AK292644 mRNA. Translation: BAF85333.1. AK300639 mRNA. Translation: BAG62328.1. AC020909 Genomic DNA. No translation available. CH471135 Genomic DNA. Translation: EAW71858.1. BC007921 mRNA. Translation: AAH07921.1.
IPI	IPI00009934. IPI00215939.
PIR	JC4839. S25655.
RefSeq	NP_001230927.1. NM_001243998.1. NP_001230928.1. NM_001243999.1. NP_001230929.1. NM_001244000.1. NP_003112.2. NM_003121.4.
UniGene	Hs.437905.
3D structure databases
ProteinModelPortal	Q01892.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q01892. 3 interactions.
STRING	9606.ENSP00000270632.
Polymorphism databases
DMDM	548971.
Proteomic databases
PaxDb	Q01892.
PRIDE	Q01892.
Protocols and materials databases
DNASU	6689.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000270632; ENSP00000270632; ENSG00000269404. ENST00000595883; ENSP00000471921; ENSG00000269404.
GeneID	6689.
KEGG	hsa:6689.
UCSC	uc002psd.3. human. uc002pse.3. human.
Organism-specific databases
CTD	6689.
GeneCards	GC19P050922.
HGNC	HGNC:11242. SPIB.
HPA	HPA018523.
MIM	606802. gene.
neXtProt	NX_Q01892.
Orphanet	186. Primary biliary cirrhosis.
PharmGKB	PA36072.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG241814.
HOGENOM	HOG000095520.
HOVERGEN	HBG002474.
InParanoid	Q01892.
KO	K09439.
OMA	QLDGPHF.
OrthoDB	EOG4FXR8B.
Gene expression databases
ArrayExpress	Q01892.
Bgee	Q01892.
CleanEx	HS_SPIB.
Genevestigator	Q01892.
GermOnline	ENSG00000142539. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000418. Ets_dom. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00178. Ets. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6689.
NextBio	26069.
SOURCE	Search...

Entry information

Entry name

SPIB_HUMAN

Accession

Primary (citable) accession number: Q01892
Secondary accession number(s): A8K9C9, B4DUG6, Q15359

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.