SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q01892

- SPIB_HUMAN

UniProt

Q01892 - SPIB_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcription factor Spi-B

Gene
SPIB
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi169 – 25284ETSAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome

GO - Biological processi

  1. cell differentiation Source: RefGenome
  2. regulation of transcription from RNA polymerase II promoter Source: RefGenome
  3. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ01892.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Spi-B
Gene namesi
Name:SPIB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:11242. SPIB.

Subcellular locationi

Isoform 1 : Nucleus 1 Publication
Isoform 2 : Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi144 – 1441S → A: Reduces interaction with IRF4 and transcriptional activation. 1 Publication
Mutagenesisi242 – 2421K → G: Abrogates DNA-binding. 1 Publication

Organism-specific databases

Orphaneti186. Primary biliary cirrhosis.
PharmGKBiPA36072.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Transcription factor Spi-BPRO_0000204136Add
BLAST

Proteomic databases

MaxQBiQ01892.
PaxDbiQ01892.
PRIDEiQ01892.

Expressioni

Tissue specificityi

Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.2 Publications

Gene expression databases

ArrayExpressiQ01892.
BgeeiQ01892.
CleanExiHS_SPIB.
GenevestigatoriQ01892.

Organism-specific databases

HPAiHPA018523.

Interactioni

Subunit structurei

Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP.2 Publications

Protein-protein interaction databases

BioGridi112567. 14 interactions.
IntActiQ01892. 3 interactions.
STRINGi9606.ENSP00000270632.

Structurei

3D structure databases

ProteinModelPortaliQ01892.
SMRiQ01892. Positions 130-251.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3131TAD1 (Acidic)Add
BLAST
Regioni41 – 6121TAD2Add
BLAST

Domaini

The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes.1 Publication

Sequence similaritiesi

Belongs to the ETS family.

Phylogenomic databases

eggNOGiNOG241814.
HOGENOMiHOG000095520.
HOVERGENiHBG002474.
InParanoidiQ01892.
KOiK09439.
PhylomeDBiQ01892.
TreeFamiTF352494.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q01892-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLALEAAQLD GPHFSCLYPD GVFYDLDSCK HSSYPDSEGA PDSLWDWTVA    50
PPVPATPYEA FDPAAAAFSH PQAAQLCYEP PTYSPAGNLE LAPSLEAPGP 100
GLPAYPTENF ASQTLVPPAY APYPSPVLSE EEDLPLDSPA LEVSDSESDE 150
ALVAGPEGKG SEAGTRKKLR LYQFLLGLLT RGDMRECVWW VEPGAGVFQF 200
SSKHKELLAR RWGQQKGNRK RMTYQKLARA LRNYAKTGEI RKVKRKLTYQ 250
FDSALLPAVR RA 262
Length:262
Mass (Da):28,819
Last modified:June 1, 1994 - v1
Checksum:iA6C21DA1BBF61B6F
GO
Isoform 2 (identifier: Q01892-2) [UniParc]FASTAAdd to Basket

Also known as: DeltaSpi-B

The sequence of this isoform differs from the canonical sequence as follows:
     163-177: AGTRKKLRLYQFLLG → GLARSCACTSSCWGY
     178-262: Missing.

Show »
Length:177
Mass (Da):18,585
Checksum:iB138A7F7706542E7
GO
Isoform 3 (identifier: Q01892-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     92-113: APSLEAPGPGLPAYPTENFASQ → MASSMTWTAASIPATLIQRGLL

Show »
Length:171
Mass (Da):19,137
Checksum:i4F0E45F5CABCB71E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti104 – 1041A → P.1 Publication
Corresponds to variant rs11546996 [ dbSNP | Ensembl ].
VAR_061150

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9191Missing in isoform 3. VSP_045124Add
BLAST
Alternative sequencei92 – 11322APSLE…NFASQ → MASSMTWTAASIPATLIQRG LL in isoform 3. VSP_045125Add
BLAST
Alternative sequencei163 – 17715AGTRK…QFLLG → GLARSCACTSSCWGY in isoform 2. VSP_001479Add
BLAST
Alternative sequencei178 – 26285Missing in isoform 2. VSP_001480Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66079 mRNA. Translation: CAA46878.1.
X96998 mRNA. Translation: CAA65726.1.
AK292644 mRNA. Translation: BAF85333.1.
AK300639 mRNA. Translation: BAG62328.1.
AC020909 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW71858.1.
BC007921 mRNA. Translation: AAH07921.1.
CCDSiCCDS33080.1. [Q01892-1]
CCDS58674.1. [Q01892-3]
CCDS59412.1. [Q01892-2]
PIRiJC4839.
S25655.
RefSeqiNP_001230927.1. NM_001243998.1. [Q01892-3]
NP_001230928.1. NM_001243999.1. [Q01892-2]
NP_001230929.1. NM_001244000.1.
NP_003112.2. NM_003121.4. [Q01892-1]
UniGeneiHs.437905.

Genome annotation databases

EnsembliENST00000270632; ENSP00000270632; ENSG00000269404. [Q01892-2]
ENST00000439922; ENSP00000391877; ENSG00000269404. [Q01892-3]
ENST00000595883; ENSP00000471921; ENSG00000269404. [Q01892-1]
GeneIDi6689.
KEGGihsa:6689.
UCSCiuc002psd.3. human. [Q01892-1]
uc002pse.3. human. [Q01892-2]
uc010ycc.2. human.

Polymorphism databases

DMDMi548971.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66079 mRNA. Translation: CAA46878.1 .
X96998 mRNA. Translation: CAA65726.1 .
AK292644 mRNA. Translation: BAF85333.1 .
AK300639 mRNA. Translation: BAG62328.1 .
AC020909 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW71858.1 .
BC007921 mRNA. Translation: AAH07921.1 .
CCDSi CCDS33080.1. [Q01892-1 ]
CCDS58674.1. [Q01892-3 ]
CCDS59412.1. [Q01892-2 ]
PIRi JC4839.
S25655.
RefSeqi NP_001230927.1. NM_001243998.1. [Q01892-3 ]
NP_001230928.1. NM_001243999.1. [Q01892-2 ]
NP_001230929.1. NM_001244000.1.
NP_003112.2. NM_003121.4. [Q01892-1 ]
UniGenei Hs.437905.

3D structure databases

ProteinModelPortali Q01892.
SMRi Q01892. Positions 130-251.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112567. 14 interactions.
IntActi Q01892. 3 interactions.
STRINGi 9606.ENSP00000270632.

Polymorphism databases

DMDMi 548971.

Proteomic databases

MaxQBi Q01892.
PaxDbi Q01892.
PRIDEi Q01892.

Protocols and materials databases

DNASUi 6689.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000270632 ; ENSP00000270632 ; ENSG00000269404 . [Q01892-2 ]
ENST00000439922 ; ENSP00000391877 ; ENSG00000269404 . [Q01892-3 ]
ENST00000595883 ; ENSP00000471921 ; ENSG00000269404 . [Q01892-1 ]
GeneIDi 6689.
KEGGi hsa:6689.
UCSCi uc002psd.3. human. [Q01892-1 ]
uc002pse.3. human. [Q01892-2 ]
uc010ycc.2. human.

Organism-specific databases

CTDi 6689.
GeneCardsi GC19P050922.
HGNCi HGNC:11242. SPIB.
HPAi HPA018523.
MIMi 606802. gene.
neXtProti NX_Q01892.
Orphaneti 186. Primary biliary cirrhosis.
PharmGKBi PA36072.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG241814.
HOGENOMi HOG000095520.
HOVERGENi HBG002474.
InParanoidi Q01892.
KOi K09439.
PhylomeDBi Q01892.
TreeFami TF352494.

Enzyme and pathway databases

SignaLinki Q01892.

Miscellaneous databases

GeneWikii SPIB.
GenomeRNAii 6689.
NextBioi 26069.
PROi Q01892.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q01892.
Bgeei Q01892.
CleanExi HS_SPIB.
Genevestigatori Q01892.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00178. Ets. 1 hit.
[Graphical view ]
PRINTSi PR00454. ETSDOMAIN.
SMARTi SM00413. ETS. 1 hit.
[Graphical view ]
PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1."
    Ray D., Bosselut R., Ghysdael J., Mattei M.-G., Tavitian A., Moreau-Gachelin F.
    Mol. Cell. Biol. 12:4297-4304(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
  2. "An alternatively spliced isoform of the Spi-B transcription factor."
    Ray-Gallet D., Tavitian A., Moreau-Gachelin F.
    Biochem. Biophys. Res. Commun. 223:257-263(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT PRO-104.
    Tissue: Rectum and Thymus.
  4. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: B-cell.
  7. "SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1."
    Rao S., Matsumura A., Yoon J., Simon M.C.
    J. Biol. Chem. 274:11115-11124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH IRF4; JUN; SPIB; SPI1 AND TBP, DOMAINS PEST; TAD1 AND TAD2, MUTAGENESIS OF SER-144 AND LYS-242.
  8. "Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells."
    Nagy M., Chapuis B., Matthes T.
    Br. J. Haematol. 116:429-435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. "Interaction between the hematopoietic Ets transcription factor Spi-B and the coactivator CREB-binding protein associated with negative cross-talk with c-Myb."
    Yamamoto H., Kihara-Negishi F., Yamada T., Suzuki M., Nakano T., Oikawa T.
    Cell Growth Differ. 13:69-75(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CREBBP AND EP300.
  10. "The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development."
    Schotte R., Rissoan M.-C., Bendriss-Vermare N., Bridon J.-M., Duhen T., Weijer K., Briere F., Spits H.
    Blood 101:1015-1023(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  11. "The ETS transcription factor Spi-B is required for human plasmacytoid dendritic cell development."
    Schotte R., Nagasawa M., Weijer K., Spits H., Blom B.
    J. Exp. Med. 200:1503-1509(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSPIB_HUMAN
AccessioniPrimary (citable) accession number: Q01892
Secondary accession number(s): A8K9C9, B4DUG6, Q15359
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi