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Q01887

- RYK_MOUSE

UniProt

Q01887 - RYK_MOUSE

Protein

Tyrosine-protein kinase RYK

Gene

Ryk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.4 Publications

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei351 – 3511ATPPROSITE-ProRule annotation
    Active sitei452 – 4521Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi323 – 3319ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. frizzled binding Source: UniProtKB
    3. protein binding Source: MGI
    4. protein kinase activity Source: InterPro
    5. Wnt-activated receptor activity Source: MGI
    6. Wnt-protein binding Source: UniProtKB

    GO - Biological processi

    1. axon guidance Source: UniProtKB
    2. axonogenesis Source: MGI
    3. commissural neuron axon guidance Source: MGI
    4. corpus callosum development Source: UniProtKB
    5. negative chemotaxis Source: MGI
    6. negative regulation of axon extension involved in axon guidance Source: MGI
    7. neuron differentiation Source: UniProtKB
    8. neuron projection development Source: UniProtKB
    9. positive regulation of MAPK cascade Source: UniProtKB
    10. skeletal system morphogenesis Source: MGI
    11. Wnt signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Receptor, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Wnt signaling pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.1. 3474.
    ReactomeiREACT_207044. TCF dependent signaling in response to WNT.
    REACT_214043. PCP/CE pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase RYK (EC:2.7.10.1)
    Alternative name(s):
    Kinase VIK
    Met-related kinase
    NYK-R
    Gene namesi
    Name:Ryk
    Synonyms:Mrk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:101766. Ryk.

    Subcellular locationi

    Membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Nucleus 1 Publication. Cytoplasm 1 Publication
    Note: In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: UniProtKB
    4. nucleus Source: UniProtKB
    5. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Sequence AnalysisAdd
    BLAST
    Chaini35 – 594560Tyrosine-protein kinase RYKPRO_0000024465Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence Analysis
    Modified residuei482 – 4821Phosphotyrosine; by autocatalysisBy similarity

    Post-translational modificationi

    Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ01887.

    PTM databases

    PhosphoSiteiQ01887.

    Expressioni

    Tissue specificityi

    Is detected in all the tissues. Highest levels are seen in the ovary, lung and placenta.

    Developmental stagei

    Highest expression at E16 when callosal axons are beginning to cross the midline. At E17-E18, when the majority of axons are projecting away from the midline, expression is observed but at a lower level. Present on the cell bodies of neurons in cortical layers at E18.2 Publications

    Gene expression databases

    ArrayExpressiQ01887.
    BgeeiQ01887.
    CleanExiMM_RYK.
    GenevestigatoriQ01887.

    Interactioni

    Subunit structurei

    Interacts with DVL1 (via PDZ domain).1 Publication

    Protein-protein interaction databases

    BioGridi203044. 11 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01887.
    SMRiQ01887. Positions 47-186, 289-585.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini35 – 211177ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini240 – 594355CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei212 – 23928HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini50 – 178129WIFPROSITE-ProRule annotationAdd
    BLAST
    Domaini317 – 590274Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The extracellular WIF domain is responsible for Wnt binding.1 Publication

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 WIF domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00720000108377.
    HOGENOMiHOG000236284.
    HOVERGENiHBG047417.
    InParanoidiQ01887.
    KOiK05128.
    OMAiDHASEVQ.
    OrthoDBiEOG7M98KK.
    PhylomeDBiQ01887.
    TreeFamiTF317402.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR003306. WIF.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF02019. WIF. 1 hit.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    ProDomiPD013948. WIF. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00219. TyrKc. 1 hit.
    SM00469. WIF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50814. WIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01887-1 [UniParc]FASTAAdd to Basket

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    MRAGRGGVPG SGGLRAPPPP LLLLLLAMLP AAAPRSPALA AAPAGPSVSL    50
    YLSEDEVRRL LGLDAELYYV RNDLISHYAL SFNLLVPSET NFLHFTWHAK 100
    SKVEYKLGFQ VDNFVAMGMP QVNISAQGEV PRTLSVFRVE LSCTGKVDSE 150
    VMILMQLNLT VNSSKNFTVL NFKRRKMCYK KLEEVKTSAL DKNTSRTIYD 200
    PVHAAPTTST RVFYISVGVC CAVIFLVAII LAVLHLHSMK RIELDDSISA 250
    SSSSQGLSQP STQTTQYLRA DTPNNATPIT SSSGYPTLRI EKNDLRSVTL 300
    LEAKAKVKDI AISRERITLK DVLQEGTFGR IFHGILVDEK DPNKEKQTFV 350
    KTVKDQASEV QVTMMLTESC KLRGLHHRNL LPITHVCIEE GEKPMVVLPY 400
    MNWGNLKLFL RQCKLVEANN PQAISQQDLV HMAIQIACGM SYLARREVIH 450
    RDLAARNCVI DDTLQVKITD NALSRDLFPM DYHCLGDNEN RPVRWMALES 500
    LVNNEFSSAS DVWAFGVTLW ELMTLGQTPY VDIDPFEMAA YLKDGYRIAQ 550
    PINCPDELFA VMACCWALDP EERPKFQQLV QCLTEFHAAL GAYV 594
    Length:594
    Mass (Da):66,285
    Last modified:June 1, 1994 - v2
    Checksum:i5C7261A3F7824520
    GO

    Sequence cautioni

    The sequence AAA40079.1 differs from that shown. Reason: Frameshift at positions 17 and 28.
    The sequence AAA40079.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171Missing in AAA02913. (PubMed:8394755)Curated
    Sequence conflicti20 – 201P → A in AAA40079. (PubMed:1334548)Curated
    Sequence conflicti31 – 311A → P in AAA40079. (PubMed:1334548)Curated
    Sequence conflicti35 – 362RS → V in AAA40079. (PubMed:1334548)Curated
    Sequence conflicti38 – 414ALAA → GPGR in AAA40079. (PubMed:1334548)Curated
    Sequence conflicti112 – 1121D → N in AAA40079. (PubMed:1334548)Curated
    Sequence conflicti130 – 1301V → G in AAA40079. (PubMed:1334548)Curated
    Sequence conflicti311 – 3111A → G in AAA40079. (PubMed:1334548)Curated
    Sequence conflicti316 – 3183RIT → GSH in AAA02913. (PubMed:8394755)Curated
    Sequence conflicti327 – 3271T → S in AAA40079. (PubMed:1334548)Curated
    Sequence conflicti341 – 3411D → R in AAA40079. (PubMed:1334548)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L02210 mRNA. Translation: AAA40579.1.
    S53216 mRNA. Translation: AAB25044.1.
    M98547 mRNA. Translation: AAA40079.1. Sequence problems.
    L38394 mRNA. Translation: AAA67060.1.
    L21707 mRNA. Translation: AAA02913.1.
    BC006963 mRNA. Translation: AAH06963.1.
    CCDSiCCDS40747.1.
    PIRiA47186.
    I56248.
    I58386.
    RefSeqiNP_001036072.1. NM_001042607.1.
    NP_038677.3. NM_013649.3.
    UniGeneiMm.335391.

    Genome annotation databases

    EnsembliENSMUST00000175883; ENSMUSP00000135858; ENSMUSG00000032547.
    GeneIDi20187.
    KEGGimmu:20187.
    UCSCiuc009rga.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L02210 mRNA. Translation: AAA40579.1 .
    S53216 mRNA. Translation: AAB25044.1 .
    M98547 mRNA. Translation: AAA40079.1 . Sequence problems.
    L38394 mRNA. Translation: AAA67060.1 .
    L21707 mRNA. Translation: AAA02913.1 .
    BC006963 mRNA. Translation: AAH06963.1 .
    CCDSi CCDS40747.1.
    PIRi A47186.
    I56248.
    I58386.
    RefSeqi NP_001036072.1. NM_001042607.1.
    NP_038677.3. NM_013649.3.
    UniGenei Mm.335391.

    3D structure databases

    ProteinModelPortali Q01887.
    SMRi Q01887. Positions 47-186, 289-585.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203044. 11 interactions.

    PTM databases

    PhosphoSitei Q01887.

    Proteomic databases

    PRIDEi Q01887.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000175883 ; ENSMUSP00000135858 ; ENSMUSG00000032547 .
    GeneIDi 20187.
    KEGGi mmu:20187.
    UCSCi uc009rga.1. mouse.

    Organism-specific databases

    CTDi 6259.
    MGIi MGI:101766. Ryk.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00720000108377.
    HOGENOMi HOG000236284.
    HOVERGENi HBG047417.
    InParanoidi Q01887.
    KOi K05128.
    OMAi DHASEVQ.
    OrthoDBi EOG7M98KK.
    PhylomeDBi Q01887.
    TreeFami TF317402.

    Enzyme and pathway databases

    BRENDAi 2.7.10.1. 3474.
    Reactomei REACT_207044. TCF dependent signaling in response to WNT.
    REACT_214043. PCP/CE pathway.

    Miscellaneous databases

    ChiTaRSi RYK. mouse.
    NextBioi 297729.
    PROi Q01887.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q01887.
    Bgeei Q01887.
    CleanExi MM_RYK.
    Genevestigatori Q01887.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR003306. WIF.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF02019. WIF. 1 hit.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    ProDomi PD013948. WIF. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00219. TyrKc. 1 hit.
    SM00469. WIF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50814. WIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the cDNA encoding a receptor tyrosine kinase-related molecule with a catalytic region homologous to c-met."
      Paul S.R., Merberg D., Finnerty H., Morris G.E., Morris J.C., Jones S.S., Kriz R., Turner K.J., Wood C.R.
      Int. J. Cell Cloning 10:309-314(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The murine vik gene (chromosome 9) encodes a putative receptor with unique protein kinase motifs."
      Kelman Z., Simon-Chazottes D., Guenet J.-L., Yarden Y.
      Oncogene 8:37-44(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "RYK, a receptor tyrosine kinase-related molecule with unusual kinase domain motifs."
      Hovens C.M., Stacker S.A., Andres A.-C., Harpur A.G., Ziemiecki A., Wilks A.F.
      Proc. Natl. Acad. Sci. U.S.A. 89:11818-11822(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Peritoneal macrophage.
    4. "The receptor tyrosine kinase-related gene (ryk) demonstrates lineage and stage-specific expression in hematopoietic cells."
      Simoneaux D.K., Fletcher F.A., Jurecic R., Shilling H.G., Van N.T., Patel P., Belmont J.W.
      J. Immunol. 154:1157-1166(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Isolation of a novel receptor tyrosine kinase cDNA expressed by developing erythroid progenitors."
      Yee K., Bishop T.R., Mather C., Zon L.I.
      Blood 82:1335-1343(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-318.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 190-594.
      Tissue: Mammary tumor.
    7. "Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth."
      Lu W., Yamamoto V., Ortega B., Baltimore D.
      Cell 119:97-108(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DOMAIN, INTERACTION WITH DVL1.
    8. "Ryk-mediated Wnt repulsion regulates posterior-directed growth of corticospinal tract."
      Liu Y., Shi J., Lu C.C., Wang Z.B., Lyuksyutova A.I., Song X.J., Zou Y.
      Nat. Neurosci. 8:1151-1159(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The Wnt receptor Ryk is required for Wnt5a-mediated axon guidance on the contralateral side of the corpus callosum."
      Keeble T.R., Halford M.M., Seaman C., Kee N., Macheda M., Anderson R.B., Stacker S.A., Cooper H.M.
      J. Neurosci. 26:5840-5848(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    10. "Cleavage of the Wnt receptor Ryk regulates neuronal differentiation during cortical neurogenesis."
      Lyu J., Yamamoto V., Lu W.
      Dev. Cell 15:773-780(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CLEAVAGE BY PRESENILIN, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiRYK_MOUSE
    AccessioniPrimary (citable) accession number: Q01887
    Secondary accession number(s): Q61890
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3