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Protein

POU domain, class 5, transcription factor 1

Gene

POU5F1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.1 Publication

Miscellaneous

Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi230 – 289HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • DNA binding Source: HGNC
  • DNA binding transcription factor activity Source: HGNC
  • miRNA binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: BHF-UCL
  • sequence-specific DNA binding Source: MGI
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
  • transcription factor binding Source: BHF-UCL
  • transcription regulatory region DNA binding Source: BHF-UCL
  • transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: BHF-UCL

GO - Biological processi

  • anatomical structure morphogenesis Source: ProtInc
  • blastocyst development Source: BHF-UCL
  • BMP signaling pathway involved in heart induction Source: BHF-UCL
  • cardiac cell fate determination Source: BHF-UCL
  • cell fate commitment involved in formation of primary germ layer Source: BHF-UCL
  • endodermal cell fate specification Source: MGI
  • mRNA transcription by RNA polymerase II Source: BHF-UCL
  • negative regulation of gene silencing by miRNA Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of catenin import into nucleus Source: BHF-UCL
  • positive regulation of SMAD protein import into nucleus Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • regulation of asymmetric cell division Source: BHF-UCL
  • regulation of gene expression Source: UniProtKB
  • regulation of heart induction by regulation of canonical Wnt signaling pathway Source: BHF-UCL
  • regulation of methylation-dependent chromatin silencing Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: HGNC
  • response to wounding Source: UniProtKB
  • somatic stem cell population maintenance Source: UniProtKB
  • transcription by RNA polymerase II Source: BHF-UCL

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-2892245 POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation
R-HSA-2892247 POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
SignaLinkiQ01860
SIGNORiQ01860

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name:
Oct-3
Octamer-binding protein 4
Short name:
Oct-4
Octamer-binding transcription factor 3
Short name:
OTF-3
Gene namesi
Name:POU5F1
Synonyms:OCT3, OCT4, OTF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204531.15
HGNCiHGNC:9221 POU5F1
MIMi164177 gene
neXtProtiNX_Q01860

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

Organism-specific databases

DisGeNETi5460
OpenTargetsiENSG00000204531
PharmGKBiPA33545

Chemistry databases

ChEMBLiCHEMBL3580526
DrugBankiDB00988 Dopamine
DB00368 Norepinephrine

Polymorphism and mutation databases

DMDMi400659

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007471 – 360POU domain, class 5, transcription factor 1Add BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111Phosphoserine; by MAPK2 Publications1
Cross-linki123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei235Phosphothreonine1 Publication1
Modified residuei236Phosphoserine1 Publication1
Modified residuei289Phosphoserine1 Publication1
Modified residuei290Phosphoserine1 Publication1
Modified residuei355Phosphoserine1 Publication1

Post-translational modificationi

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).By similarity
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.By similarity
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.2 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ01860
MaxQBiQ01860
PaxDbiQ01860
PeptideAtlasiQ01860
PRIDEiQ01860

PTM databases

iPTMnetiQ01860
PhosphoSitePlusiQ01860

Expressioni

Tissue specificityi

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.2 Publications

Developmental stagei

Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.1 Publication

Inductioni

Transcriptional activity is positively regulated by PKM.1 Publication

Gene expression databases

BgeeiENSG00000204531
ExpressionAtlasiQ01860 baseline and differential
GenevisibleiQ01860 HS

Organism-specific databases

HPAiCAB025600
CAB026380
HPA058267

Interactioni

Subunit structurei

Interacts with UBE2I and ZSCAN10 (By similarity). Interacts with PKM. Interacts with WWP2. Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (By similarity). Interacts with ZNF322 (By similarity).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • transcription factor binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111456, 317 interactors
CORUMiQ01860
DIPiDIP-33440N
ELMiQ01860
IntActiQ01860, 17 interactors
MINTiQ01860
STRINGi9606.ENSP00000259915

Structurei

3D structure databases

ProteinModelPortaliQ01860
SMRiQ01860
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 212POU-specificPROSITE-ProRule annotationAdd BLAST75

Domaini

The POU-specific domain mediates interaction with PKM.1 Publication

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
GeneTreeiENSGT00760000118935
HOVERGENiHBG057998
InParanoidiQ01860
KOiK09367
OMAiLENMFLQ
OrthoDBiEOG091G0U0S
PhylomeDBiQ01860
TreeFamiTF316413

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR015585 POU_dom_5
PANTHERiPTHR11636:SF86 PTHR11636:SF86, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q01860-1) [UniParc]FASTAAdd to basket
Also known as: Oct-3A, Oct3A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGPGIGPG
60 70 80 90 100
VGPGSEVWGI PPCPPPYEFC GGMAYCGPQV GVGLVPQGGL ETSQPEGEAG
110 120 130 140 150
VGVESNSDGA SPEPCTVTPG AVKLEKEKLE QNPEESQDIK ALQKELEQFA
160 170 180 190 200
KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL
210 220 230 240 250
RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLENLFL
260 270 280 290 300
QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYAQREDFEA
310 320 330 340 350
AGSPFSGGPV SFPLAPGPHF GTPGYGSPHF TALYSSVPFP EGEAFPPVSV
360
TTLGSPMHSN
Length:360
Mass (Da):38,571
Last modified:July 1, 1993 - v1
Checksum:i934C58DAEA0C535B
GO
Isoform B (identifier: Q01860-2) [UniParc]FASTAAdd to basket
Also known as: Oct-3B, Oct3B

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: MAGHLASDFA...KEKLEQNPEE → MHFYRLFLGA...LTSLLPFKIQ

Show »
Length:265
Mass (Da):30,085
Checksum:i41C21E136EFBCBB2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189A → G in CAA79974 (PubMed:7908264).Curated1
Sequence conflicti220I → T in CAA79974 (PubMed:7908264).Curated1
Sequence conflicti227V → L in CAA79974 (PubMed:7908264).Curated1
Sequence conflicti230R → G in CAA79975 (PubMed:7908264).Curated1
Sequence conflicti240R → Q in CAA79975 (PubMed:7908264).Curated1
Sequence conflicti251Q → R in CAA79975 (PubMed:7908264).Curated1
Sequence conflicti272D → DVVR in AAB35990 (PubMed:8567814).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_046203226L → F. Corresponds to variant dbSNP:rs1150767Ensembl.1
Natural variantiVAR_003774322T → A1 Publication1
Natural variantiVAR_046204351T → I. Corresponds to variant dbSNP:rs1061120Ensembl.1
Natural variantiVAR_003775357M → L1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0023331 – 135MAGHL…QNPEE → MHFYRLFLGATRRFLNPEWK GEIDNWCVYVLTSLLPFKIQ in isoform B. 1 PublicationAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11898 mRNA Translation: CAA77951.1
Z11899 mRNA Translation: CAA77952.1
GQ472773 Genomic DNA Translation: ACZ95700.1
AB088113 Genomic DNA Translation: BAC54946.1
AB202105 Genomic DNA Translation: BAE78630.1
AB103619 Genomic DNA Translation: BAF31281.1
BA000025 Genomic DNA Translation: BAB63311.1
AL662833 Genomic DNA No translation available.
AL662844 Genomic DNA No translation available.
AL773544 Genomic DNA No translation available.
BX088580 Genomic DNA No translation available.
CR759815 Genomic DNA No translation available.
CR388229 Genomic DNA No translation available.
CR847794 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03367.1
CH471081 Genomic DNA Translation: EAX03368.1
BC117435 mRNA Translation: AAI17436.1
BC117437 mRNA Translation: AAI17438.1
Z21963 mRNA Translation: CAA79974.1
Z21964 mRNA Translation: CAA79975.1
S81255 mRNA Translation: AAB35990.1
CCDSiCCDS34391.1 [Q01860-1]
PIRiS25561
S32652
RefSeqiNP_001272916.1, NM_001285987.1 [Q01860-2]
NP_002692.2, NM_002701.5 [Q01860-1]
UniGeneiHs.249184
Hs.632482
Hs.646545

Genome annotation databases

EnsembliENST00000259915; ENSP00000259915; ENSG00000204531 [Q01860-1]
ENST00000376243; ENSP00000365419; ENSG00000206454 [Q01860-2]
ENST00000383524; ENSP00000373016; ENSG00000206454 [Q01860-1]
ENST00000412166; ENSP00000387646; ENSG00000229094 [Q01860-2]
ENST00000419095; ENSP00000413622; ENSG00000235068 [Q01860-2]
ENST00000429314; ENSP00000387619; ENSG00000237582 [Q01860-2]
ENST00000429603; ENSP00000392877; ENSG00000233911 [Q01860-1]
ENST00000433063; ENSP00000405041; ENSG00000235068 [Q01860-1]
ENST00000433348; ENSP00000412665; ENSG00000230336 [Q01860-2]
ENST00000434616; ENSP00000388842; ENSG00000229094 [Q01860-1]
ENST00000437747; ENSP00000391681; ENSG00000237582 [Q01860-1]
ENST00000451077; ENSP00000391507; ENSG00000233911 [Q01860-2]
ENST00000454714; ENSP00000400047; ENSG00000230336 [Q01860-1]
GeneIDi5460
KEGGihsa:5460
UCSCiuc003nsv.4 human [Q01860-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPO5F1_HUMAN
AccessioniPrimary (citable) accession number: Q01860
Secondary accession number(s): A6NCS1
, A6NLL8, D2IYK4, P31359, Q15167, Q15168, Q16422, Q5STF3, Q5STF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 28, 2018
This is version 192 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health