Q01855 (RS15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S15

Alternative name(s):
RIG protein
RP52
S21
YS21

Gene names

Name:	RPS15
Synonyms:	RPS21
Ordered Locus Names:	YOL040C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the nuclear export of the small ribosomal subunit. Has a role in the late stage of the assembly of pre-40S particles within the nucleus and controls their export to the cytoplasm. Ref.7
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.5
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribosomal protein S19P family.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Non-traceable author statement Ref.5. Source: SGD rRNA export from nucleus Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	cytosolic small ribosomal subunit Non-traceable author statement Ref.5. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Non-traceable author statement Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 142	141	40S ribosomal protein S15		PRO_0000130051
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.6
	Modified residue	16	1	Phosphoserine Ref.8
	Modified residue	124	1	Phosphothreonine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q01855 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 06B564FD68051CD2	FASTA	142	16,002
	10 20 30 40 50 60 MSQAVNAKKR VFKTHSYRGV DLEKLLEMST EDFVKLAPAR VRRRFARGMT SKPAGFMKKL 70 80 90 100 110 120 RAAKLAAPEN EKPAPVRTHM RNMIIVPEMI GSVVGIYNGK AFNQVEIRPE MLGHYLGEFS 130 140 ITYTPVRHGR AGATTSRFIP LK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural determination of Saccharomyces cerevisiae rig gene and identification of its product as ribosomal protein S21." Takasawa S., Tohgo A., Unno M., Yonekura H., Okamoto H. FEBS Lett. 307:318-323(1992) [PubMed: 1644188] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[6]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[7]	"The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast." Leger-Silvestre I., Milkereit P., Ferreira-Cerca S., Saveanu C., Rousselle J.-C., Choesmel V., Guinefoleau C., Gas N., Gleizes P.-E. EMBO J. 23:2336-2347(2004) [PubMed: 15167894] [Abstract] Cited for: FUNCTION.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-124, MASS SPECTROMETRY.
[10]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 47-126, ELECTRON MICROSCOPY.
[11]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 47-126, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D11386 mRNA. Translation: BAA01982.1.
D11387 Genomic DNA. Translation: BAA01983.1.
Z74782 Genomic DNA. Translation: CAA99042.1.
AY558426 Genomic DNA. Translation: AAS56752.1.
BK006948 Genomic DNA. Translation: DAA10742.1.

PIR

S24053.

RefSeq

NP_014602.1. NM_001183294.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	S	47-126	[»]
1S1H	electron microscopy	11.70	S	47-126	[»]
3IZB	electron microscopy	-	R	1-142	[»]
3O2Z	X-ray	4.00	I	1-142	[»]
3O30	X-ray	4.00	I	1-142	[»]

ProteinModelPortal

Q01855.

SMR

Q01855. Positions 47-117.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q01855. 3 interactions.

STRING

Q01855.

Proteomic databases

PeptideAtlas

Q01855.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOL040C; YOL040C; YOL040C.

GeneID

854117.

KEGG

sce:YOL040C.

NMPDR

fig|4932.3.peg.5694.

Organism-specific databases

CYGD

YOL040c.

SGD

S000005400. RPS15.

Phylogenomic databases

eggNOG

fuNOG09065.

GeneTree

EFGT00050000006600.

HOGENOM

HBG307515.

OMA

THCRDMI.

OrthoDB

EOG4HHSBV.

Gene expression databases

ArrayExpress

Q01855.

Genevestigator

Q01855.

GermOnline

YOL040C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002222. Ribosomal_S19.
IPR020934. Ribosomal_S19_CS.
IPR023575. Ribosomal_S19_SF.
IPR005713. Ribosomal_S19A/S15e.
[Graphical view]

Gene3D

G3DSA:3.30.860.10. Ribosomal_S19. 1 hit.

KO

K02958.

PANTHER

PTHR11880. Ribosomal_S19. 1 hit.

Pfam

PF00203. Ribosomal_S19. 1 hit.
[Graphical view]

PIRSF

PIRSF002144. Ribosomal_S19. 1 hit.

PRINTS

PR00975. RIBOSOMALS19.

SUPFAM

SSF54570. Ribosomal_S19. 1 hit.

TIGRFAMs

TIGR01025. RpsS_arch. 1 hit.

PROSITE

PS00323. RIBOSOMAL_S19. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

975816.

Entry information

Entry name

RS15_YEAST

Accession

Primary (citable) accession number: Q01855
Secondary accession number(s): D6W226

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	December 14, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program