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Protein

Mitochondrial import inner membrane translocase subunit TIM44

Gene

TIM44

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 and its co-chaperone (MGE1) to drive protein translocation into the matrix using ATP as an energy source.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi101 – 1088ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chaperone binding Source: SGD
  • protein binding, bridging Source: SGD

GO - Biological processi

  • protein import into mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31297-MONOMER.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM44
Alternative name(s):
Inner membrane import site protein 45
Short name:
ISP45
Membrane import machinery protein MIM44
Mitochondrial protein import protein 1
Gene namesi
Name:TIM44
Synonyms:ISP45, MIM44, MPI1
Ordered Locus Names:YIL022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL022W.
SGDiS000001284. TIM44.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 431Mitochondrial import inner membrane translocase subunit TIM44PRO_0000034319
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiQ01852.
PeptideAtlasiQ01852.

Interactioni

Subunit structurei

Component of the PAM complex, at least composed of SSC1 (mtHsp70), MGE1, TIM44, PAM16/TIM16, PAM17 and PAM18/TIM14. Forms part of the receptor complex that consists of at least 3 different proteins (TIM17, TIM23, TIM44).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PAM16P429493EBI-9141,EBI-26019
SSC1P0CS903EBI-9141,EBI-8637
TIM17P395152EBI-9141,EBI-9127
TIM23P328978EBI-9141,EBI-9136

GO - Molecular functioni

  • chaperone binding Source: SGD
  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34966. 27 interactions.
DIPiDIP-694N.
IntActiQ01852. 20 interactions.
MINTiMINT-535200.

Structurei

Secondary structure

1
431
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi237 – 2459Combined sources
Helixi249 – 2579Combined sources
Turni259 – 2624Combined sources
Helixi271 – 2744Combined sources
Helixi286 – 29510Combined sources
Helixi297 – 30711Combined sources
Helixi310 – 3167Combined sources
Helixi319 – 33315Combined sources
Turni334 – 3363Combined sources
Beta strandi337 – 3393Combined sources
Beta strandi342 – 35615Combined sources
Beta strandi358 – 3603Combined sources
Beta strandi363 – 37412Combined sources
Beta strandi377 – 3804Combined sources
Beta strandi386 – 3883Combined sources
Beta strandi394 – 40411Combined sources
Beta strandi406 – 4083Combined sources
Beta strandi412 – 4143Combined sources
Beta strandi417 – 4237Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FXTX-ray3.20A234-425[»]
3QK9X-ray3.10A/B210-431[»]
ProteinModelPortaliQ01852.
SMRiQ01852. Positions 234-425.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01852.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim44 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000000051.
HOGENOMiHOG000208172.
InParanoidiQ01852.
KOiK17804.
OMAiHDFTQNP.
OrthoDBiEOG780RWX.

Family and domain databases

InterProiIPR032710. NTF2-like_dom.
IPR017303. Tim44.
IPR007379. Tim44-like_dom.
[Graphical view]
PfamiPF04280. Tim44. 1 hit.
[Graphical view]
PIRSFiPIRSF037871. TIM44. 1 hit.
SMARTiSM00978. Tim44. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRSTFIRTS GTSSRTLTAR YRSQYTGLLV ARVLFSTSTT RAQGGNPRSP
60 70 80 90 100
LQIFRDTFKK EWEKSQELQE NIKTLQDASG KLGESEAYKK AREAYLKAQR
110 120 130 140 150
GSTIVGKTLK KTGETMEHIA TKAWESELGK NTRKAAAATA KKLDESFEPV
160 170 180 190 200
RQTKIYKEVS EVIDDGESSR YGGFITKEQR RLKRERDLAS GKRHRAVKSN
210 220 230 240 250
EDAGTAVVAT NIESKESFGK KVEDFKEKTV VGRSIQSLKN KLWDESENPL
260 270 280 290 300
IVVMRKITNK VGGFFAETES SRVYSQFKLM DPTFSNESFT RHLREYIVPE
310 320 330 340 350
ILEAYVKGDV KVLKKWFSEA PFNVYAAQQK IFKEQDVYAD GRILDIRGVE
360 370 380 390 400
IVSAKLLAPQ DIPVLVVGCR AQEINLYRKK KTGEIAAGDE ANILMSSYAM
410 420 430
VFTRDPEQID DDETEGWKIL EFVRGGSRQF T
Length:431
Mass (Da):48,854
Last modified:July 1, 1993 - v1
Checksum:i8E98C738178B1E44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46881 Genomic DNA. Translation: CAA86970.1.
X67276 Genomic DNA. Translation: CAA47693.1.
BK006942 Genomic DNA. Translation: DAA08523.1.
PIRiS25196.
RefSeqiNP_012242.1. NM_001179372.1.

Genome annotation databases

EnsemblFungiiYIL022W; YIL022W; YIL022W.
GeneIDi854790.
KEGGisce:YIL022W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46881 Genomic DNA. Translation: CAA86970.1.
X67276 Genomic DNA. Translation: CAA47693.1.
BK006942 Genomic DNA. Translation: DAA08523.1.
PIRiS25196.
RefSeqiNP_012242.1. NM_001179372.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FXTX-ray3.20A234-425[»]
3QK9X-ray3.10A/B210-431[»]
ProteinModelPortaliQ01852.
SMRiQ01852. Positions 234-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34966. 27 interactions.
DIPiDIP-694N.
IntActiQ01852. 20 interactions.
MINTiMINT-535200.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBiQ01852.
PeptideAtlasiQ01852.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL022W; YIL022W; YIL022W.
GeneIDi854790.
KEGGisce:YIL022W.

Organism-specific databases

EuPathDBiFungiDB:YIL022W.
SGDiS000001284. TIM44.

Phylogenomic databases

GeneTreeiENSGT00390000000051.
HOGENOMiHOG000208172.
InParanoidiQ01852.
KOiK17804.
OMAiHDFTQNP.
OrthoDBiEOG780RWX.

Enzyme and pathway databases

BioCyciYEAST:G3O-31297-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ01852.
NextBioi977582.
PROiQ01852.

Family and domain databases

InterProiIPR032710. NTF2-like_dom.
IPR017303. Tim44.
IPR007379. Tim44-like_dom.
[Graphical view]
PfamiPF04280. Tim44. 1 hit.
[Graphical view]
PIRSFiPIRSF037871. TIM44. 1 hit.
SMARTiSM00978. Tim44. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria."
    Maarse A.C., Blom J., Grivell L.A., Meijer M.
    EMBO J. 11:3619-3628(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204510 / AB320.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Protein import into yeast mitochondria: the inner membrane import site protein ISP45 is the MPI1 gene product."
    Horst M., Jenoe P., Kronidou N.G., Bolliger L., Oppliger W., Scherer P., Manning-Krieg U., Jascur T., Schatz G.
    EMBO J. 12:3035-3041(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
  5. "Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor."
    van der Laan M., Chacinska A., Lind M., Perschil I., Sickmann A., Meyer H.E., Guiard B., Meisinger C., Pfanner N., Rehling P.
    Mol. Cell. Biol. 25:7449-7458(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PAM COMPLEX WITH PAM16; PAM17; PAM18; MGE1 AND SSC1.

Entry informationi

Entry nameiTIM44_YEAST
AccessioniPrimary (citable) accession number: Q01852
Secondary accession number(s): D6VVQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.