Q01842 (POK_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ets DNA-binding protein pokkuri

Alternative name(s):
Protein anterior open
Protein yan

Gene names

Name:	aop
Synonyms:	pok, Yan
ORF Names:	CG3166

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of photoreceptor development that acts antagonistically to the proneural signal mediated by RAS. It acts upstream of SINA to inhibit R7 development. Ref.1 Ref.2
Subcellular location	Nucleus. Note: In undifferentiated cells during the early stages of eye development. Ref.1
Tissue specificity	Expressed in R7 and cone cells of the eye. Ref.1
Developmental stage	Expressed in the embryo. Ref.1
Miscellaneous	'Pokkuri' means 'dropping dead' in Japanese. Flies lacking aop result in the differentiation of supernumerary photoreceptors in the eye.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.
Sequence caution	The sequence BAA01080.1 differs from that shown. Reason: Frameshift at positions 181, 213, 250, 271, 275, 278, 284, 552, 574, 695 and 697.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	JNK cascade Non-traceable author statement. Source: FlyBase R3/R4 cell fate commitment Inferred from mutant phenotype. Source: FlyBase R7 cell development Traceable author statement. Source: FlyBase border follicle cell migration Inferred from mutant phenotype. Source: FlyBase cell fate determination Inferred from mutant phenotype. Source: FlyBase dendrite morphogenesis Inferred from mutant phenotype. Source: FlyBase dorsal closure Non-traceable author statement. Source: FlyBase endocytosis Inferred from mutant phenotype. Source: FlyBase gonad development Inferred from mutant phenotype. Source: FlyBase induction of apoptosis Inferred from mutant phenotype. Source: FlyBase muscle fiber development Inferred from mutant phenotype. Source: FlyBase muscle organ development Inferred from mutant phenotype. Source: FlyBase negative regulation of Ras protein signal transduction Non-traceable author statement. Source: FlyBase negative regulation of photoreceptor cell differentiation Traceable author statement. Source: FlyBase negative regulation of transcription, DNA-dependent Traceable author statement. Source: FlyBase oenocyte delamination Inferred from mutant phenotype. Source: FlyBase positive regulation of R7 cell differentiation Inferred from genetic interaction. Source: FlyBase regulation of hemocyte differentiation Inferred from mutant phenotype. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: FlyBase
Molecular function	protein binding Inferred from physical interaction. Source: FlyBase sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Non-traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 732

732

Ets DNA-binding protein pokkuri

PRO_0000204131

Regions

Domain

33 – 117

85

PNT

DNA binding

396 – 479

84

ETS

Compositional bias

313 – 322

10

Poly-Gln

Compositional bias

332 – 349

18

Gln-rich

Compositional bias

528 – 539

12

Gln-rich

Amino acid modifications

Modified residue

543

1

Phosphoserine Ref.6

Modified residue

677

1

Phosphoserine Ref.6

Modified residue

682

1

Phosphoserine Ref.6

Modified residue

696

1

Phosphoserine Ref.6

Experimental info

Sequence conflict

27

1

R → C in BAA01080. Ref.2

Sequence conflict

505

1

G → S in BAA01080. Ref.2

Sequence conflict

527

1

P → A in BAA01080. Ref.2

Sequence conflict

567

1

P → R in AAQ23555. Ref.5

Sequence conflict

614

1

P → S in BAA01080. Ref.2

Sequence conflict

693

1

S → P in AAQ23555. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

732

Helix Strand Turn

Details...

Helix

51 – 53

3

Helix

56 – 69

14

Helix

77 – 80

4

Helix

84 – 87

4

Helix

92 – 98

7

Turn

100 – 102

3

Helix

103 – 118

16

Sequences

	Sequence		Length	Mass (Da)
	Q01842 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 25D36A652BA67176	FASTA	732	78,574
	10 20 30 40 50 60 MSKMKMLPVQ LSLNSLNPGI WSDVLWRCPP APSSQLAELK TQLPPSLPSD PRLWSREDVL 70 80 90 100 110 120 VFLRFCVREF DLPKLDFDLF QMNGKALCLL TRADFGHRCP GAGDVLHNVL QMLIIESHMM 130 140 150 160 170 180 QWHLPNSPVT PTSRYPLSPH SHPPTPTWPP LNAPPENSPF HSSAHSLAGH HFMAPNSVTL 190 200 210 220 230 240 SPPPSVDSQA SSPPQAPYQN GGATGAAPGS AGGSAPAAGG ATNTSNPTSS SASSTGSNGS 250 260 270 280 290 300 QPNIMPMKGI SSASSNHSDS EEEYSETSGG VSKMPPAPLS YSTASPPGTP ILKDIKPNWT 310 320 330 340 350 360 QQLTNSFVNS WSQQQQQQQQ QQAAAVAAVA AQAQQHQLQQ QQQQQQLPQK LTLDNTAGPV 370 380 390 400 410 420 VTPAGGSISA PTTPSYMYKA KREFFPENSE PNTNGRLLWD FLQQLLNDRN QKYSDLIAWK 430 440 450 460 470 480 CRDTGVFKIV DPAGLAKLWG IQKNHLSMNY DKMSRALRYY YRVNILRKVQ GERHCYQFLR 490 500 510 520 530 540 NPTELKNIKN ISLLRQSTPA NGNGGSPSMP QGSSQAPGSP AGQNWNPQQQ SQQQQQSPQR 550 560 570 580 590 600 PASRNGPMSL PAVAAVAAAA AAAYGPPPTS PLFMHAINGA FHYLSAAAAG PPPNSPALNT 610 620 630 640 650 660 PSAVGGPDKF QFHPLKLENG SGSGSESAGE DLKPTDLSVS SKSTATSNED CYPLIRNADG 670 680 690 700 710 720 LTTIKLIRYN EHQVAASPAG QSPKHDDQQA GASNASSSPR PMDQASEQAQ PVPMESDCNG 730 GESEDSFRHM QQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Negative control of photoreceptor development in Drosophila by the product of the yan gene, an ETS domain protein." Lai Z.C., Rubin G.M. Cell 70:609-620(1992) [PubMed: 1505027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Pokkuri, a Drosophila gene encoding an E-26-specific (Ets) domain protein, prevents overproduction of the R7 photoreceptor." Tei H., Nihonmatsu I., Yokokura T., Ueda R., Sano Y., Okuda T., Sato K., Hirata K., Fujita S.C., Yamamoto D. Proc. Natl. Acad. Sci. U.S.A. 89:6856-6860(1992) [PubMed: 1495974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-543; SER-677; SER-682 AND SER-696, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M97693 mRNA. Translation: AAA29023.1.
D10228 mRNA. Translation: BAA01080.1. Frameshift.
AE014134 Genomic DNA. Translation: AAF51297.1.
AE014134 Genomic DNA. Translation: AAN10445.1.
BT010237 mRNA. Translation: AAQ23555.1.
BT015272 mRNA. Translation: AAT94501.1.

PIR

A43315.
A46193.

RefSeq

NP_523455.2. NM_078731.2.
NP_722766.1. NM_164457.1.

UniGene

Dm.267.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SV0	X-ray	2.07	A/B	42-118	[»]
1SV4	X-ray	2.15	A/B	42-118	[»]

ProteinModelPortal

Q01842.

SMR

Q01842. Positions 12-118, 395-481.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-20916N.

IntAct

Q01842. 2 interactions.

MINT

MINT-771736.

STRING

Q01842.

Proteomic databases

PRIDE

Q01842.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0077850; FBpp0077522; FBgn0000097.
FBtr0077851; FBpp0077523; FBgn0000097.

GeneID

33392.

KEGG

dme:Dmel_CG3166.

Organism-specific databases

CTD

33392.

FlyBase

FBgn0000097. aop.

Phylogenomic databases

eggNOG

inNOG08023.

GeneTree

EMGT00050000000490.

InParanoid

Q01842.

OMA

IAWKCRD.

OrthoDB

EOG463XTK.

PhylomeDB

Q01842.

Gene expression databases

ArrayExpress

Q01842.

Bgee

Q01842.

GermOnline

CG3166. Drosophila melanogaster.

Family and domain databases

InterPro

IPR000418. Ets.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.150.50. SAM_type. 1 hit.
G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

KO

K03211.

Pfam

PF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]

PRINTS

PR00454. ETSDOMAIN.

SMART

SM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]

SUPFAM

SSF47769. SAM_homology. 1 hit.

PROSITE

PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

783322.

Entry information

Entry name

POK_DROME

Accession

Primary (citable) accession number: Q01842
Secondary accession number(s): Q6AWH6, Q6NR43, Q9VQ81

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project