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Protein

Ets DNA-binding protein pokkuri

Gene

aop

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of photoreceptor development that acts antagonistically to the proneural signal mediated by RAS. It acts upstream of SINA to inhibit R7 development.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi396 – 479ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

  • protein domain specific binding Source: FlyBase
  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: FlyBase
  • transcription factor activity, sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • border follicle cell migration Source: FlyBase
  • cell fate determination Source: FlyBase
  • compound eye development Source: FlyBase
  • compound eye photoreceptor cell differentiation Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • dorsal closure Source: FlyBase
  • endocytosis Source: FlyBase
  • gonad development Source: FlyBase
  • JNK cascade Source: FlyBase
  • MAPK cascade Source: FlyBase
  • muscle fiber development Source: FlyBase
  • muscle organ development Source: FlyBase
  • negative regulation of cell differentiation Source: FlyBase
  • negative regulation of fusion cell fate specification Source: FlyBase
  • negative regulation of photoreceptor cell differentiation Source: FlyBase
  • negative regulation of Ras protein signal transduction Source: FlyBase
  • negative regulation of terminal cell fate specification, open tracheal system Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • negative regulation of Wnt signaling pathway Source: FlyBase
  • neuron development Source: FlyBase
  • oenocyte delamination Source: FlyBase
  • positive regulation of R7 cell differentiation Source: FlyBase
  • R3/R4 cell fate commitment Source: FlyBase
  • R7 cell development Source: FlyBase
  • regulation of establishment of planar polarity Source: FlyBase
  • regulation of hemocyte differentiation Source: FlyBase
  • regulation of neurogenesis Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ01842.

Names & Taxonomyi

Protein namesi
Recommended name:
Ets DNA-binding protein pokkuri
Alternative name(s):
Protein anterior open
Protein yan
Gene namesi
Name:aop
Synonyms:pok, Yan
ORF Names:CG3166
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000097. aop.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

  • Note: In undifferentiated cells during the early stages of eye development.

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: FlyBase
  • nucleoplasm Source: FlyBase
  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041311 – 732Ets DNA-binding protein pokkuriAdd BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei543Phosphoserine1 Publication1
Modified residuei677Phosphoserine1 Publication1
Modified residuei682Phosphoserine1 Publication1
Modified residuei696Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ01842.
PRIDEiQ01842.

PTM databases

iPTMnetiQ01842.

Expressioni

Tissue specificityi

Expressed in R7 and cone cells of the eye.1 Publication

Developmental stagei

Expressed in the embryo.1 Publication

Gene expression databases

BgeeiFBgn0000097.
ExpressionAtlasiQ01842. baseline.
GenevisibleiQ01842. DM.

Interactioni

GO - Molecular functioni

  • protein domain specific binding Source: FlyBase

Protein-protein interaction databases

BioGridi59621. 44 interactors.
DIPiDIP-20916N.
IntActiQ01842. 2 interactors.
MINTiMINT-771736.
STRINGi7227.FBpp0077522.

Structurei

Secondary structure

1732
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi51 – 53Combined sources3
Helixi56 – 69Combined sources14
Helixi77 – 80Combined sources4
Helixi84 – 87Combined sources4
Helixi92 – 98Combined sources7
Turni100 – 102Combined sources3
Helixi103 – 118Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SV0X-ray2.07A/B42-118[»]
1SV4X-ray2.15A/B42-118[»]
ProteinModelPortaliQ01842.
SMRiQ01842.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01842.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 117PNTPROSITE-ProRule annotationAdd BLAST85

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi313 – 322Poly-Gln10
Compositional biasi332 – 349Gln-richAdd BLAST18
Compositional biasi528 – 539Gln-richAdd BLAST12

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000044763.
InParanoidiQ01842.
KOiK03211.
OMAiIAWKCRD.
OrthoDBiEOG091G07P9.
PhylomeDBiQ01842.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR033077. Pokkuri.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF201. PTHR11849:SF201. 4 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01842-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKMKMLPVQ LSLNSLNPGI WSDVLWRCPP APSSQLAELK TQLPPSLPSD
60 70 80 90 100
PRLWSREDVL VFLRFCVREF DLPKLDFDLF QMNGKALCLL TRADFGHRCP
110 120 130 140 150
GAGDVLHNVL QMLIIESHMM QWHLPNSPVT PTSRYPLSPH SHPPTPTWPP
160 170 180 190 200
LNAPPENSPF HSSAHSLAGH HFMAPNSVTL SPPPSVDSQA SSPPQAPYQN
210 220 230 240 250
GGATGAAPGS AGGSAPAAGG ATNTSNPTSS SASSTGSNGS QPNIMPMKGI
260 270 280 290 300
SSASSNHSDS EEEYSETSGG VSKMPPAPLS YSTASPPGTP ILKDIKPNWT
310 320 330 340 350
QQLTNSFVNS WSQQQQQQQQ QQAAAVAAVA AQAQQHQLQQ QQQQQQLPQK
360 370 380 390 400
LTLDNTAGPV VTPAGGSISA PTTPSYMYKA KREFFPENSE PNTNGRLLWD
410 420 430 440 450
FLQQLLNDRN QKYSDLIAWK CRDTGVFKIV DPAGLAKLWG IQKNHLSMNY
460 470 480 490 500
DKMSRALRYY YRVNILRKVQ GERHCYQFLR NPTELKNIKN ISLLRQSTPA
510 520 530 540 550
NGNGGSPSMP QGSSQAPGSP AGQNWNPQQQ SQQQQQSPQR PASRNGPMSL
560 570 580 590 600
PAVAAVAAAA AAAYGPPPTS PLFMHAINGA FHYLSAAAAG PPPNSPALNT
610 620 630 640 650
PSAVGGPDKF QFHPLKLENG SGSGSESAGE DLKPTDLSVS SKSTATSNED
660 670 680 690 700
CYPLIRNADG LTTIKLIRYN EHQVAASPAG QSPKHDDQQA GASNASSSPR
710 720 730
PMDQASEQAQ PVPMESDCNG GESEDSFRHM QQ
Length:732
Mass (Da):78,574
Last modified:June 1, 2001 - v2
Checksum:i25D36A652BA67176
GO

Sequence cautioni

The sequence BAA01080 differs from that shown. Reason: Frameshift at positions 181, 213, 250, 271, 275, 278, 284, 552, 574, 695 and 697.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27R → C in BAA01080 (PubMed:1495974).Curated1
Sequence conflicti505G → S in BAA01080 (PubMed:1495974).Curated1
Sequence conflicti527P → A in BAA01080 (PubMed:1495974).Curated1
Sequence conflicti567P → R in AAQ23555 (Ref. 5) Curated1
Sequence conflicti614P → S in BAA01080 (PubMed:1495974).Curated1
Sequence conflicti693S → P in AAQ23555 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97693 mRNA. Translation: AAA29023.1.
D10228 mRNA. Translation: BAA01080.1. Frameshift.
AE014134 Genomic DNA. Translation: AAF51297.1.
AE014134 Genomic DNA. Translation: AAN10445.1.
BT010237 mRNA. Translation: AAQ23555.1.
BT015272 mRNA. Translation: AAT94501.1.
PIRiA43315.
A46193.
RefSeqiNP_001259908.1. NM_001272979.1.
NP_001259909.1. NM_001272980.1.
NP_001259910.1. NM_001272981.1.
NP_523455.2. NM_078731.3.
NP_722766.1. NM_164457.2.
UniGeneiDm.267.

Genome annotation databases

EnsemblMetazoaiFBtr0077850; FBpp0077522; FBgn0000097.
FBtr0077851; FBpp0077523; FBgn0000097.
FBtr0330658; FBpp0303508; FBgn0000097.
FBtr0330659; FBpp0303509; FBgn0000097.
FBtr0330660; FBpp0303510; FBgn0000097.
GeneIDi33392.
KEGGidme:Dmel_CG3166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97693 mRNA. Translation: AAA29023.1.
D10228 mRNA. Translation: BAA01080.1. Frameshift.
AE014134 Genomic DNA. Translation: AAF51297.1.
AE014134 Genomic DNA. Translation: AAN10445.1.
BT010237 mRNA. Translation: AAQ23555.1.
BT015272 mRNA. Translation: AAT94501.1.
PIRiA43315.
A46193.
RefSeqiNP_001259908.1. NM_001272979.1.
NP_001259909.1. NM_001272980.1.
NP_001259910.1. NM_001272981.1.
NP_523455.2. NM_078731.3.
NP_722766.1. NM_164457.2.
UniGeneiDm.267.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SV0X-ray2.07A/B42-118[»]
1SV4X-ray2.15A/B42-118[»]
ProteinModelPortaliQ01842.
SMRiQ01842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59621. 44 interactors.
DIPiDIP-20916N.
IntActiQ01842. 2 interactors.
MINTiMINT-771736.
STRINGi7227.FBpp0077522.

PTM databases

iPTMnetiQ01842.

Proteomic databases

PaxDbiQ01842.
PRIDEiQ01842.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077850; FBpp0077522; FBgn0000097.
FBtr0077851; FBpp0077523; FBgn0000097.
FBtr0330658; FBpp0303508; FBgn0000097.
FBtr0330659; FBpp0303509; FBgn0000097.
FBtr0330660; FBpp0303510; FBgn0000097.
GeneIDi33392.
KEGGidme:Dmel_CG3166.

Organism-specific databases

CTDi33392.
FlyBaseiFBgn0000097. aop.

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000044763.
InParanoidiQ01842.
KOiK03211.
OMAiIAWKCRD.
OrthoDBiEOG091G07P9.
PhylomeDBiQ01842.

Enzyme and pathway databases

SignaLinkiQ01842.

Miscellaneous databases

ChiTaRSiaop. fly.
EvolutionaryTraceiQ01842.
GenomeRNAii33392.
PROiQ01842.

Gene expression databases

BgeeiFBgn0000097.
ExpressionAtlasiQ01842. baseline.
GenevisibleiQ01842. DM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR033077. Pokkuri.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF201. PTHR11849:SF201. 4 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOK_DROME
AccessioniPrimary (citable) accession number: Q01842
Secondary accession number(s): Q6AWH6, Q6NR43, Q9VQ81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 173 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

'Pokkuri' means 'dropping dead' in Japanese. Flies lacking aop result in the differentiation of supernumerary photoreceptors in the eye.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.