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Protein

Ets DNA-binding protein pokkuri

Gene

aop

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ets-related protein that functions as a negative regulator of photoreceptor development acting antagonistically to pnt and the proneural signal mediated by RAS (PubMed:1505027, PubMed:1495974, PubMed:8033205). It acts upstream of SINA to inhibit R7 development (PubMed:1505027, PubMed:1495974).3 Publications

Miscellaneous

'Pokkuri' means 'dropping dead' in Japanese. Flies lacking aop result in the differentiation of supernumerary photoreceptors in the eye.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi396 – 479ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

  • DNA binding transcription factor activity Source: FlyBase
  • protein domain specific binding Source: FlyBase
  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: FlyBase

GO - Biological processi

  • border follicle cell migration Source: FlyBase
  • cell fate determination Source: FlyBase
  • compound eye development Source: FlyBase
  • compound eye photoreceptor cell differentiation Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • dorsal closure Source: FlyBase
  • endocytosis Source: FlyBase
  • gonad development Source: FlyBase
  • JNK cascade Source: FlyBase
  • MAPK cascade Source: FlyBase
  • muscle fiber development Source: FlyBase
  • negative regulation of cell differentiation Source: FlyBase
  • negative regulation of fusion cell fate specification Source: FlyBase
  • negative regulation of non-canonical Wnt signaling pathway Source: FlyBase
  • negative regulation of photoreceptor cell differentiation Source: FlyBase
  • negative regulation of Ras protein signal transduction Source: FlyBase
  • negative regulation of terminal cell fate specification, open tracheal system Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: FlyBase
  • oenocyte delamination Source: FlyBase
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of R7 cell differentiation Source: FlyBase
  • R3/R4 cell fate commitment Source: FlyBase
  • R7 cell development Source: FlyBase
  • regulation of establishment of planar polarity Source: FlyBase
  • regulation of hemocyte differentiation Source: FlyBase
  • regulation of neurogenesis Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SignaLinkiQ01842

Names & Taxonomyi

Protein namesi
Recommended name:
Ets DNA-binding protein pokkuri
Alternative name(s):
Protein anterior open
Protein yan
Gene namesi
Name:aop
Synonyms:pok, Yan
ORF Names:CG3166
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000097 aop

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041311 – 732Ets DNA-binding protein pokkuriAdd BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei543Phosphoserine1 Publication1
Modified residuei677Phosphoserine1 Publication1
Modified residuei682Phosphoserine1 Publication1
Modified residuei696Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated in response to MAPK signaling. May be phosphorylated by rl.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ01842
PRIDEiQ01842

PTM databases

iPTMnetiQ01842

Expressioni

Tissue specificityi

Expressed in R7 and cone cells of the eye.1 Publication

Developmental stagei

Expressed in the embryo.1 Publication

Gene expression databases

BgeeiFBgn0000097
ExpressionAtlasiQ01842 differential
GenevisibleiQ01842 DM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
edlQ7K1192EBI-161142,EBI-153260

GO - Molecular functioni

  • protein domain specific binding Source: FlyBase

Protein-protein interaction databases

BioGridi5962159 interactors.
DIPiDIP-20916N
IntActiQ01842 6 interactors.
STRINGi7227.FBpp0077522

Structurei

Secondary structure

1732
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi51 – 53Combined sources3
Helixi56 – 69Combined sources14
Helixi77 – 80Combined sources4
Helixi84 – 87Combined sources4
Helixi92 – 98Combined sources7
Turni100 – 102Combined sources3
Helixi103 – 118Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SV0X-ray2.07A/B42-118[»]
1SV4X-ray2.15A/B42-118[»]
ProteinModelPortaliQ01842
SMRiQ01842
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01842

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 117PNTPROSITE-ProRule annotationAdd BLAST85

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi313 – 322Poly-Gln10
Compositional biasi332 – 349Gln-richAdd BLAST18
Compositional biasi528 – 539Gln-richAdd BLAST12

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3804 Eukaryota
ENOG4111K4J LUCA
GeneTreeiENSGT00760000118996
HOGENOMiHOG000044763
InParanoidiQ01842
KOiK03211
OMAiIAWKCRD
OrthoDBiEOG091G07P9
PhylomeDBiQ01842

Family and domain databases

Gene3Di1.10.10.101 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR003118 Pointed_dom
IPR033077 Pokkuri
IPR013761 SAM/pointed_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11849:SF201 PTHR11849:SF201, 2 hits
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PF02198 SAM_PNT, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SM00251 SAM_PNT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit
PS51433 PNT, 1 hit

Sequencei

Sequence statusi: Complete.

Q01842-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKMKMLPVQ LSLNSLNPGI WSDVLWRCPP APSSQLAELK TQLPPSLPSD
60 70 80 90 100
PRLWSREDVL VFLRFCVREF DLPKLDFDLF QMNGKALCLL TRADFGHRCP
110 120 130 140 150
GAGDVLHNVL QMLIIESHMM QWHLPNSPVT PTSRYPLSPH SHPPTPTWPP
160 170 180 190 200
LNAPPENSPF HSSAHSLAGH HFMAPNSVTL SPPPSVDSQA SSPPQAPYQN
210 220 230 240 250
GGATGAAPGS AGGSAPAAGG ATNTSNPTSS SASSTGSNGS QPNIMPMKGI
260 270 280 290 300
SSASSNHSDS EEEYSETSGG VSKMPPAPLS YSTASPPGTP ILKDIKPNWT
310 320 330 340 350
QQLTNSFVNS WSQQQQQQQQ QQAAAVAAVA AQAQQHQLQQ QQQQQQLPQK
360 370 380 390 400
LTLDNTAGPV VTPAGGSISA PTTPSYMYKA KREFFPENSE PNTNGRLLWD
410 420 430 440 450
FLQQLLNDRN QKYSDLIAWK CRDTGVFKIV DPAGLAKLWG IQKNHLSMNY
460 470 480 490 500
DKMSRALRYY YRVNILRKVQ GERHCYQFLR NPTELKNIKN ISLLRQSTPA
510 520 530 540 550
NGNGGSPSMP QGSSQAPGSP AGQNWNPQQQ SQQQQQSPQR PASRNGPMSL
560 570 580 590 600
PAVAAVAAAA AAAYGPPPTS PLFMHAINGA FHYLSAAAAG PPPNSPALNT
610 620 630 640 650
PSAVGGPDKF QFHPLKLENG SGSGSESAGE DLKPTDLSVS SKSTATSNED
660 670 680 690 700
CYPLIRNADG LTTIKLIRYN EHQVAASPAG QSPKHDDQQA GASNASSSPR
710 720 730
PMDQASEQAQ PVPMESDCNG GESEDSFRHM QQ
Length:732
Mass (Da):78,574
Last modified:June 1, 2001 - v2
Checksum:i25D36A652BA67176
GO

Sequence cautioni

The sequence BAA01080 differs from that shown. Reason: Frameshift at positions 181, 213, 250, 271, 275, 278, 284, 552, 574, 695 and 697.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27R → C in BAA01080 (PubMed:1495974).Curated1
Sequence conflicti505G → S in BAA01080 (PubMed:1495974).Curated1
Sequence conflicti527P → A in BAA01080 (PubMed:1495974).Curated1
Sequence conflicti567P → R in AAQ23555 (Ref. 5) Curated1
Sequence conflicti614P → S in BAA01080 (PubMed:1495974).Curated1
Sequence conflicti693S → P in AAQ23555 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97693 mRNA Translation: AAA29023.1
D10228 mRNA Translation: BAA01080.1 Frameshift.
AE014134 Genomic DNA Translation: AAF51297.1
AE014134 Genomic DNA Translation: AAN10445.1
BT010237 mRNA Translation: AAQ23555.1
BT015272 mRNA Translation: AAT94501.1
PIRiA43315
A46193
RefSeqiNP_001259908.1, NM_001272979.1
NP_001259909.1, NM_001272980.1
NP_001259910.1, NM_001272981.1
NP_523455.2, NM_078731.3
NP_722766.1, NM_164457.2
UniGeneiDm.267

Genome annotation databases

EnsemblMetazoaiFBtr0077850; FBpp0077522; FBgn0000097
FBtr0077851; FBpp0077523; FBgn0000097
FBtr0330658; FBpp0303508; FBgn0000097
FBtr0330659; FBpp0303509; FBgn0000097
FBtr0330660; FBpp0303510; FBgn0000097
GeneIDi33392
KEGGidme:Dmel_CG3166

Similar proteinsi

Entry informationi

Entry nameiPOK_DROME
AccessioniPrimary (citable) accession number: Q01842
Secondary accession number(s): Q6AWH6, Q6NR43, Q9VQ81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 187 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome