Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q01827

- VMAT2_RAT

UniProt

Q01827 - VMAT2_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Synaptic vesicular amine transporter

Gene

Slc18a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

GO - Molecular functioni

  1. amine transmembrane transporter activity Source: RGD
  2. drug binding Source: RGD
  3. drug transmembrane transporter activity Source: InterPro
  4. enzyme binding Source: RGD
  5. heat shock protein binding Source: RGD
  6. serotonin transmembrane transporter activity Source: RGD

GO - Biological processi

  1. aging Source: RGD
  2. cellular response to ammonium ion Source: RGD
  3. cellular response to drug Source: RGD
  4. death Source: Ensembl
  5. endocytic recycling Source: RGD
  6. glucose homeostasis Source: RGD
  7. insulin secretion Source: RGD
  8. locomotory behavior Source: Ensembl
  9. monoamine transport Source: RGD
  10. negative regulation of neurotransmitter transport Source: RGD
  11. neurotransmitter transport Source: RGD
  12. post-embryonic development Source: Ensembl
  13. response to amphetamine Source: RGD
  14. response to cocaine Source: RGD
  15. response to corticosterone Source: RGD
  16. response to drug Source: RGD
  17. response to herbicide Source: RGD
  18. response to metal ion Source: RGD
  19. response to starvation Source: RGD
  20. response to zinc ion Source: RGD
  21. serotonin transport Source: GOC
  22. synaptic vesicle amine transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name:
VAT2
Gene namesi
Name:Slc18a2
Synonyms:Svat, Vmat2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi3694. Slc18a2.

Subcellular locationi

GO - Cellular componenti

  1. axon terminus Source: RGD
  2. cell body Source: RGD
  3. cell projection Source: RGD
  4. cytoplasm Source: RGD
  5. dense core granule Source: RGD
  6. integral component of membrane Source: UniProtKB-KW
  7. neuronal cell body Source: RGD
  8. neuron projection Source: RGD
  9. synaptic vesicle Source: RGD
  10. synaptic vesicle membrane Source: RGD
  11. terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Synaptic vesicular amine transporterPRO_0000127516Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi118 ↔ 325By similarity
Modified residuei512 – 5121Phosphoserine; by CK21 Publication
Modified residuei514 – 5141Phosphoserine; by CK21 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ01827.
PRIDEiQ01827.

PTM databases

PhosphoSiteiQ01827.

Expressioni

Tissue specificityi

Brainstem and stomach.

Gene expression databases

GenevestigatoriQ01827.

Interactioni

Subunit structurei

Interacts with SLC6A3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011983.

Structurei

3D structure databases

ProteinModelPortaliQ01827.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini42 – 13089Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini152 – 1609CytoplasmicSequence Analysis
Topological domaini182 – 1909Lumenal, vesicleSequence Analysis
Topological domaini212 – 2209CytoplasmicSequence Analysis
Topological domaini244 – 2496Lumenal, vesicleSequence Analysis
Topological domaini273 – 29220CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini313 – 32917Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini354 – 3585CytoplasmicSequence Analysis
Topological domaini380 – 39011Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini412 – 4154CytoplasmicSequence Analysis
Topological domaini437 – 4415Lumenal, vesicleSequence Analysis
Topological domaini464 – 51552CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121HelicalSequence AnalysisAdd
BLAST
Transmembranei131 – 15121HelicalSequence AnalysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence AnalysisAdd
BLAST
Transmembranei191 – 21121HelicalSequence AnalysisAdd
BLAST
Transmembranei221 – 24323HelicalSequence AnalysisAdd
BLAST
Transmembranei250 – 27223HelicalSequence AnalysisAdd
BLAST
Transmembranei293 – 31220HelicalSequence AnalysisAdd
BLAST
Transmembranei330 – 35324HelicalSequence AnalysisAdd
BLAST
Transmembranei359 – 37921HelicalSequence AnalysisAdd
BLAST
Transmembranei391 – 41121HelicalSequence AnalysisAdd
BLAST
Transmembranei416 – 43621HelicalSequence AnalysisAdd
BLAST
Transmembranei442 – 46322HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG255549.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ01827.
KOiK08155.
OrthoDBiEOG79CXZ7.
PhylomeDBiQ01827.
TreeFamiTF313494.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01827-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS
60 70 80 90 100
IKHEKNSTEI QTTRPELVVS TSESIFSYYN NSTVLITGNA TGTLPGGQSH
110 120 130 140 150
KATSTQHTVA NTTVPSDCPS EDRDLLNENV QVGLLFASKA TVQLLTNPFI
160 170 180 190 200
GLLTNRIGYP IPMFAGFCIM FISTVMFAFS SSYAFLLIAR SLQGIGSSCS
210 220 230 240 250
SVAGMGMLAS VYTDDEERGK PMGIALGGLA MGVLVGPPFG SVLYEFVGKT
260 270 280 290 300
APFLVLAALV LLDGAIQLFV LQPSRVQPES QKGTPLTTLL KDPYILIAAG
310 320 330 340 350
SICFANMGIA MLEPALPIWM METMCSRKWQ LGVAFLPASI SYLIGTNIFG
360 370 380 390 400
ILAHKMGRWL CALLGMVIVG ISILCIPFAK NIYGLIAPNF GVGFAIGMVD
410 420 430 440 450
SSMMPIMGYL VDLRHVSVYG SVYAIADVAF CMGYAIGPSA GGAIAKAIGF
460 470 480 490 500
PWLMTIIGII DIAFAPLCFF LRSPPAKEEK MAILMDHNCP IKRKMYTQNN
510
VQSYPIGDDE ESESD
Length:515
Mass (Da):55,690
Last modified:September 19, 2003 - v2
Checksum:i627A904C1D35D552
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2212KP → NA in AAA41627. (PubMed:1438304)Curated
Sequence conflicti397 – 3971G → F in AAA42190. (PubMed:1505023)Curated
Sequence conflicti493 – 4931R → T in AAA41627. (PubMed:1438304)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97381 mRNA. Translation: AAA42190.1.
L00603 mRNA. Translation: AAA41627.1.
PIRiA46374.
RefSeqiNP_037163.1. NM_013031.1.
XP_006231726.1. XM_006231664.2.
UniGeneiRn.9686.

Genome annotation databases

EnsembliENSRNOT00000011983; ENSRNOP00000011983; ENSRNOG00000008890.
GeneIDi25549.
KEGGirno:25549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97381 mRNA. Translation: AAA42190.1 .
L00603 mRNA. Translation: AAA41627.1 .
PIRi A46374.
RefSeqi NP_037163.1. NM_013031.1.
XP_006231726.1. XM_006231664.2.
UniGenei Rn.9686.

3D structure databases

ProteinModelPortali Q01827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000011983.

Chemistry

BindingDBi Q01827.
ChEMBLi CHEMBL4828.

PTM databases

PhosphoSitei Q01827.

Proteomic databases

PaxDbi Q01827.
PRIDEi Q01827.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000011983 ; ENSRNOP00000011983 ; ENSRNOG00000008890 .
GeneIDi 25549.
KEGGi rno:25549.

Organism-specific databases

CTDi 6571.
RGDi 3694. Slc18a2.

Phylogenomic databases

eggNOGi NOG255549.
GeneTreei ENSGT00390000003851.
HOGENOMi HOG000018545.
HOVERGENi HBG055082.
InParanoidi Q01827.
KOi K08155.
OrthoDBi EOG79CXZ7.
PhylomeDBi Q01827.
TreeFami TF313494.

Miscellaneous databases

NextBioi 607093.
PROi Q01827.

Gene expression databases

Genevestigatori Q01827.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 2 hits.
TIGRFAMsi TIGR00880. 2_A_01_02. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A cDNA that suppresses MPP+ toxicity encodes a vesicular amine transporter."
    Liu Y., Peter D., Roghani A., Schuldiner S., Prive G.G., Eisenberg D., Brecha N., Edwards R.H.
    Cell 70:539-551(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression cloning of a reserpine-sensitive vesicular monoamine transporter."
    Erickson J.D., Eiden L.E., Hoffman B.J.
    Proc. Natl. Acad. Sci. U.S.A. 89:10993-10997(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Expression and regulation of a vesicular monoamine transporter in rat stomach: a putative histamine transporter."
    Dimaline R., Struthers J.
    J. Physiol. (Lond.) 490:249-256(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Gastric corpus.
  4. "Phosphorylation of a vesicular monoamine transporter by casein kinase II."
    Krantz D.E., Peter D., Lui Y., Edwards R.H.
    J. Biol. Chem. 272:6752-6759(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-512 AND SER-514.

Entry informationi

Entry nameiVMAT2_RAT
AccessioniPrimary (citable) accession number: Q01827
Secondary accession number(s): Q9QVP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 19, 2003
Last modified: October 29, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3