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Protein

Synaptic vesicular amine transporter

Gene

Slc18a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

GO - Molecular functioni

  • amine transmembrane transporter activity Source: RGD
  • drug binding Source: RGD
  • drug transmembrane transporter activity Source: InterPro
  • enzyme binding Source: RGD
  • heat shock protein binding Source: RGD
  • serotonin transmembrane transporter activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • aminergic neurotransmitter loading into synaptic vesicle Source: RGD
  • cellular response to ammonium ion Source: RGD
  • cellular response to drug Source: RGD
  • endocytic recycling Source: RGD
  • glucose homeostasis Source: RGD
  • insulin secretion Source: RGD
  • monoamine transport Source: RGD
  • negative regulation of neurotransmitter transport Source: RGD
  • neurotransmitter transport Source: RGD
  • response to amphetamine Source: RGD
  • response to cocaine Source: RGD
  • response to corticosterone Source: RGD
  • response to drug Source: RGD
  • response to herbicide Source: RGD
  • response to metal ion Source: RGD
  • response to starvation Source: RGD
  • response to zinc ion Source: RGD

Keywordsi

Biological processNeurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name:
VAT2
Gene namesi
Name:Slc18a2
Synonyms:Svat, Vmat2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3694. Slc18a2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Topological domaini42 – 130Lumenal, vesicleSequence analysisAdd BLAST89
Transmembranei131 – 151HelicalSequence analysisAdd BLAST21
Topological domaini152 – 160CytoplasmicSequence analysis9
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Topological domaini182 – 190Lumenal, vesicleSequence analysis9
Transmembranei191 – 211HelicalSequence analysisAdd BLAST21
Topological domaini212 – 220CytoplasmicSequence analysis9
Transmembranei221 – 243HelicalSequence analysisAdd BLAST23
Topological domaini244 – 249Lumenal, vesicleSequence analysis6
Transmembranei250 – 272HelicalSequence analysisAdd BLAST23
Topological domaini273 – 292CytoplasmicSequence analysisAdd BLAST20
Transmembranei293 – 312HelicalSequence analysisAdd BLAST20
Topological domaini313 – 329Lumenal, vesicleSequence analysisAdd BLAST17
Transmembranei330 – 353HelicalSequence analysisAdd BLAST24
Topological domaini354 – 358CytoplasmicSequence analysis5
Transmembranei359 – 379HelicalSequence analysisAdd BLAST21
Topological domaini380 – 390Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei391 – 411HelicalSequence analysisAdd BLAST21
Topological domaini412 – 415CytoplasmicSequence analysis4
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
Topological domaini437 – 441Lumenal, vesicleSequence analysis5
Transmembranei442 – 463HelicalSequence analysisAdd BLAST22
Topological domaini464 – 515CytoplasmicSequence analysisAdd BLAST52

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4828.
GuidetoPHARMACOLOGYi1012.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275161 – 515Synaptic vesicular amine transporterAdd BLAST515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi80N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi81N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi111N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi118 ↔ 325By similarity
Modified residuei512Phosphoserine; by CK2Combined sources1 Publication1
Modified residuei514Phosphoserine; by CK2Combined sources1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ01827.
PRIDEiQ01827.

PTM databases

iPTMnetiQ01827.
PhosphoSitePlusiQ01827.

Expressioni

Tissue specificityi

Brainstem and stomach.

Interactioni

Subunit structurei

Interacts with SLC6A3.By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD
  • heat shock protein binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011983.

Chemistry databases

BindingDBiQ01827.

Structurei

3D structure databases

ProteinModelPortaliQ01827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ01827.
KOiK08155.
PhylomeDBiQ01827.
TreeFamiTF313494.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiView protein in InterPro
IPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
PfamiView protein in Pfam
PF07690. MFS_1. 1 hit.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiView protein in PROSITE
PS50850. MFS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS
60 70 80 90 100
IKHEKNSTEI QTTRPELVVS TSESIFSYYN NSTVLITGNA TGTLPGGQSH
110 120 130 140 150
KATSTQHTVA NTTVPSDCPS EDRDLLNENV QVGLLFASKA TVQLLTNPFI
160 170 180 190 200
GLLTNRIGYP IPMFAGFCIM FISTVMFAFS SSYAFLLIAR SLQGIGSSCS
210 220 230 240 250
SVAGMGMLAS VYTDDEERGK PMGIALGGLA MGVLVGPPFG SVLYEFVGKT
260 270 280 290 300
APFLVLAALV LLDGAIQLFV LQPSRVQPES QKGTPLTTLL KDPYILIAAG
310 320 330 340 350
SICFANMGIA MLEPALPIWM METMCSRKWQ LGVAFLPASI SYLIGTNIFG
360 370 380 390 400
ILAHKMGRWL CALLGMVIVG ISILCIPFAK NIYGLIAPNF GVGFAIGMVD
410 420 430 440 450
SSMMPIMGYL VDLRHVSVYG SVYAIADVAF CMGYAIGPSA GGAIAKAIGF
460 470 480 490 500
PWLMTIIGII DIAFAPLCFF LRSPPAKEEK MAILMDHNCP IKRKMYTQNN
510
VQSYPIGDDE ESESD
Length:515
Mass (Da):55,690
Last modified:September 19, 2003 - v2
Checksum:i627A904C1D35D552
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti220 – 221KP → NA in AAA41627 (PubMed:1438304).Curated2
Sequence conflicti397G → F in AAA42190 (PubMed:1505023).Curated1
Sequence conflicti493R → T in AAA41627 (PubMed:1438304).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97381 mRNA. Translation: AAA42190.1.
L00603 mRNA. Translation: AAA41627.1.
PIRiA46374.
RefSeqiNP_037163.1. NM_013031.1.
XP_006231726.1. XM_006231664.3.
XP_017444313.1. XM_017588824.1.
UniGeneiRn.9686.

Genome annotation databases

GeneIDi25549.
KEGGirno:25549.

Similar proteinsi

Entry informationi

Entry nameiVMAT2_RAT
AccessioniPrimary (citable) accession number: Q01827
Secondary accession number(s): Q9QVP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 19, 2003
Last modified: August 30, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families