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Q01827

- VMAT2_RAT

UniProt

Q01827 - VMAT2_RAT

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Protein
Synaptic vesicular amine transporter
Gene
Slc18a2, Svat, Vmat2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

GO - Molecular functioni

  1. amine transmembrane transporter activity Source: RGD
  2. drug binding Source: RGD
  3. drug transmembrane transporter activity Source: InterPro
  4. enzyme binding Source: RGD
  5. heat shock protein binding Source: RGD
  6. serotonin transmembrane transporter activity Source: RGD

GO - Biological processi

  1. aging Source: RGD
  2. cellular response to ammonium ion Source: RGD
  3. cellular response to drug Source: RGD
  4. death Source: Ensembl
  5. endocytic recycling Source: RGD
  6. glucose homeostasis Source: RGD
  7. insulin secretion Source: RGD
  8. locomotory behavior Source: Ensembl
  9. monoamine transport Source: RGD
  10. negative regulation of neurotransmitter transport Source: RGD
  11. neurotransmitter transport Source: RGD
  12. post-embryonic development Source: Ensembl
  13. response to amphetamine Source: RGD
  14. response to cocaine Source: RGD
  15. response to corticosterone Source: RGD
  16. response to drug Source: RGD
  17. response to herbicide Source: RGD
  18. response to metal ion Source: RGD
  19. response to starvation Source: RGD
  20. response to zinc ion Source: RGD
  21. serotonin transport Source: GOC
  22. synaptic vesicle amine transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name:
VAT2
Gene namesi
Name:Slc18a2
Synonyms:Svat, Vmat2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi3694. Slc18a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei21 – 4121Helical; Reviewed prediction
Add
BLAST
Topological domaini42 – 13089Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei131 – 15121Helical; Reviewed prediction
Add
BLAST
Topological domaini152 – 1609Cytoplasmic Reviewed prediction
Transmembranei161 – 18121Helical; Reviewed prediction
Add
BLAST
Topological domaini182 – 1909Lumenal, vesicle Reviewed prediction
Transmembranei191 – 21121Helical; Reviewed prediction
Add
BLAST
Topological domaini212 – 2209Cytoplasmic Reviewed prediction
Transmembranei221 – 24323Helical; Reviewed prediction
Add
BLAST
Topological domaini244 – 2496Lumenal, vesicle Reviewed prediction
Transmembranei250 – 27223Helical; Reviewed prediction
Add
BLAST
Topological domaini273 – 29220Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei293 – 31220Helical; Reviewed prediction
Add
BLAST
Topological domaini313 – 32917Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei330 – 35324Helical; Reviewed prediction
Add
BLAST
Topological domaini354 – 3585Cytoplasmic Reviewed prediction
Transmembranei359 – 37921Helical; Reviewed prediction
Add
BLAST
Topological domaini380 – 39011Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei391 – 41121Helical; Reviewed prediction
Add
BLAST
Topological domaini412 – 4154Cytoplasmic Reviewed prediction
Transmembranei416 – 43621Helical; Reviewed prediction
Add
BLAST
Topological domaini437 – 4415Lumenal, vesicle Reviewed prediction
Transmembranei442 – 46322Helical; Reviewed prediction
Add
BLAST
Topological domaini464 – 51552Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. axon terminus Source: RGD
  2. cell body Source: RGD
  3. cell projection Source: RGD
  4. cytoplasm Source: RGD
  5. dense core granule Source: RGD
  6. integral component of membrane Source: UniProtKB-KW
  7. neuron projection Source: RGD
  8. neuronal cell body Source: RGD
  9. synaptic vesicle Source: RGD
  10. synaptic vesicle membrane Source: RGD
  11. terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Synaptic vesicular amine transporter
PRO_0000127516Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi80 – 801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi81 – 811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi89 – 891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi111 – 1111N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi118 ↔ 325 By similarity
Modified residuei512 – 5121Phosphoserine; by CK21 Publication
Modified residuei514 – 5141Phosphoserine; by CK21 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ01827.
PRIDEiQ01827.

PTM databases

PhosphoSiteiQ01827.

Expressioni

Tissue specificityi

Brainstem and stomach.

Gene expression databases

GenevestigatoriQ01827.

Interactioni

Subunit structurei

Interacts with SLC6A3 By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011983.

Structurei

3D structure databases

ProteinModelPortaliQ01827.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG255549.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ01827.
KOiK08155.
OrthoDBiEOG79CXZ7.
PhylomeDBiQ01827.
TreeFamiTF313494.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01827-1 [UniParc]FASTAAdd to Basket

« Hide

MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS    50
IKHEKNSTEI QTTRPELVVS TSESIFSYYN NSTVLITGNA TGTLPGGQSH 100
KATSTQHTVA NTTVPSDCPS EDRDLLNENV QVGLLFASKA TVQLLTNPFI 150
GLLTNRIGYP IPMFAGFCIM FISTVMFAFS SSYAFLLIAR SLQGIGSSCS 200
SVAGMGMLAS VYTDDEERGK PMGIALGGLA MGVLVGPPFG SVLYEFVGKT 250
APFLVLAALV LLDGAIQLFV LQPSRVQPES QKGTPLTTLL KDPYILIAAG 300
SICFANMGIA MLEPALPIWM METMCSRKWQ LGVAFLPASI SYLIGTNIFG 350
ILAHKMGRWL CALLGMVIVG ISILCIPFAK NIYGLIAPNF GVGFAIGMVD 400
SSMMPIMGYL VDLRHVSVYG SVYAIADVAF CMGYAIGPSA GGAIAKAIGF 450
PWLMTIIGII DIAFAPLCFF LRSPPAKEEK MAILMDHNCP IKRKMYTQNN 500
VQSYPIGDDE ESESD 515
Length:515
Mass (Da):55,690
Last modified:September 19, 2003 - v2
Checksum:i627A904C1D35D552
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2212KP → NA in AAA41627. 1 Publication
Sequence conflicti397 – 3971G → F in AAA42190. 1 Publication
Sequence conflicti493 – 4931R → T in AAA41627. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97381 mRNA. Translation: AAA42190.1.
L00603 mRNA. Translation: AAA41627.1.
PIRiA46374.
RefSeqiNP_037163.1. NM_013031.1.
XP_006231726.1. XM_006231664.1.
XP_006231727.1. XM_006231665.1.
UniGeneiRn.9686.

Genome annotation databases

EnsembliENSRNOT00000011983; ENSRNOP00000011983; ENSRNOG00000008890.
GeneIDi25549.
KEGGirno:25549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97381 mRNA. Translation: AAA42190.1 .
L00603 mRNA. Translation: AAA41627.1 .
PIRi A46374.
RefSeqi NP_037163.1. NM_013031.1.
XP_006231726.1. XM_006231664.1.
XP_006231727.1. XM_006231665.1.
UniGenei Rn.9686.

3D structure databases

ProteinModelPortali Q01827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000011983.

Chemistry

BindingDBi Q01827.
ChEMBLi CHEMBL4828.

PTM databases

PhosphoSitei Q01827.

Proteomic databases

PaxDbi Q01827.
PRIDEi Q01827.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000011983 ; ENSRNOP00000011983 ; ENSRNOG00000008890 .
GeneIDi 25549.
KEGGi rno:25549.

Organism-specific databases

CTDi 6571.
RGDi 3694. Slc18a2.

Phylogenomic databases

eggNOGi NOG255549.
GeneTreei ENSGT00390000003851.
HOGENOMi HOG000018545.
HOVERGENi HBG055082.
InParanoidi Q01827.
KOi K08155.
OrthoDBi EOG79CXZ7.
PhylomeDBi Q01827.
TreeFami TF313494.

Miscellaneous databases

NextBioi 607093.
PROi Q01827.

Gene expression databases

Genevestigatori Q01827.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 2 hits.
TIGRFAMsi TIGR00880. 2_A_01_02. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A cDNA that suppresses MPP+ toxicity encodes a vesicular amine transporter."
    Liu Y., Peter D., Roghani A., Schuldiner S., Prive G.G., Eisenberg D., Brecha N., Edwards R.H.
    Cell 70:539-551(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression cloning of a reserpine-sensitive vesicular monoamine transporter."
    Erickson J.D., Eiden L.E., Hoffman B.J.
    Proc. Natl. Acad. Sci. U.S.A. 89:10993-10997(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Expression and regulation of a vesicular monoamine transporter in rat stomach: a putative histamine transporter."
    Dimaline R., Struthers J.
    J. Physiol. (Lond.) 490:249-256(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Gastric corpus.
  4. "Phosphorylation of a vesicular monoamine transporter by casein kinase II."
    Krantz D.E., Peter D., Lui Y., Edwards R.H.
    J. Biol. Chem. 272:6752-6759(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-512 AND SER-514.

Entry informationi

Entry nameiVMAT2_RAT
AccessioniPrimary (citable) accession number: Q01827
Secondary accession number(s): Q9QVP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 19, 2003
Last modified: April 16, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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