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Q01827 (VMAT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name=VAT2
Gene names
Name:Slc18a2
Synonyms:Svat, Vmat2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

Subunit structure

Interacts with SLC6A3 By similarity.

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein.

Tissue specificity

Brainstem and stomach.

Sequence similarities

Belongs to the major facilitator superfamily. Vesicular transporter family.

Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentCytoplasmic vesicle
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from direct assay PubMed 17582657. Source: RGD

cellular response to ammonium ion

Inferred from expression pattern PubMed 20534840. Source: RGD

cellular response to drug

Inferred from direct assay PubMed 20007701. Source: RGD

death

Inferred from electronic annotation. Source: Compara

drug transmembrane transport

Inferred from electronic annotation. Source: GOC

endocytic recycling

Inferred from expression pattern PubMed 20534840. Source: RGD

glucose homeostasis

Inferred from direct assay PubMed 18577569. Source: RGD

insulin secretion

Inferred from direct assay PubMed 18577569. Source: RGD

locomotory behavior

Inferred from electronic annotation. Source: Compara

negative regulation of neurotransmitter transport

Inferred from mutant phenotype PubMed 16926160. Source: RGD

neurotransmitter transport

Inferred from direct assay PubMed 19008227. Source: RGD

post-embryonic development

Inferred from electronic annotation. Source: Compara

response to amphetamine

Inferred from expression pattern PubMed 21159748. Source: RGD

response to cocaine

Inferred from expression pattern PubMed 19038779. Source: RGD

response to corticosterone stimulus

Inferred from expression pattern PubMed 19494803. Source: RGD

response to herbicide

Inferred from expression pattern PubMed 20553223. Source: RGD

response to starvation

Inferred from expression pattern PubMed 17536021. Source: RGD

response to zinc ion

Inferred from expression pattern PubMed 20553223. Source: RGD

synaptic vesicle amine transport

Inferred from mutant phenotype PubMed 19903816. Source: RGD

   Cellular_componentdense core granule

Inferred from direct assay PubMed 7568015. Source: RGD

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuronal cell body

Inferred from direct assay PubMed 19457116. Source: RGD

synaptic vesicle membrane

Inferred from direct assay PubMed 19038779. Source: RGD

terminal bouton

Inferred from direct assay PubMed 20534840. Source: RGD

   Molecular_functiondrug binding

Inferred from physical interaction PubMed 20007701. Source: RGD

drug transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

serotonin transmembrane transporter activity

Inferred from direct assay PubMed 20007701. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 515515Synaptic vesicular amine transporter
PRO_0000127516

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Potential
Topological domain42 – 13089Lumenal, vesicle Potential
Transmembrane131 – 15121Helical; Potential
Topological domain152 – 1609Cytoplasmic Potential
Transmembrane161 – 18121Helical; Potential
Topological domain182 – 1909Lumenal, vesicle Potential
Transmembrane191 – 21121Helical; Potential
Topological domain212 – 2209Cytoplasmic Potential
Transmembrane221 – 24323Helical; Potential
Topological domain244 – 2496Lumenal, vesicle Potential
Transmembrane250 – 27223Helical; Potential
Topological domain273 – 29220Cytoplasmic Potential
Transmembrane293 – 31220Helical; Potential
Topological domain313 – 32917Lumenal, vesicle Potential
Transmembrane330 – 35324Helical; Potential
Topological domain354 – 3585Cytoplasmic Potential
Transmembrane359 – 37921Helical; Potential
Topological domain380 – 39011Lumenal, vesicle Potential
Transmembrane391 – 41121Helical; Potential
Topological domain412 – 4154Cytoplasmic Potential
Transmembrane416 – 43621Helical; Potential
Topological domain437 – 4415Lumenal, vesicle Potential
Transmembrane442 – 46322Helical; Potential
Topological domain464 – 51552Cytoplasmic Potential

Amino acid modifications

Modified residue5121Phosphoserine; by CK2 Ref.4
Modified residue5141Phosphoserine; by CK2 Ref.4
Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation801N-linked (GlcNAc...) Potential
Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation1111N-linked (GlcNAc...) Potential
Disulfide bond118 ↔ 325 By similarity

Experimental info

Sequence conflict220 – 2212KP → NA in AAA41627. Ref.2
Sequence conflict3971G → F in AAA42190. Ref.1
Sequence conflict4931R → T in AAA41627. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q01827 [UniParc].

Last modified September 19, 2003. Version 2.
Checksum: 627A904C1D35D552

FASTA51555,690
        10         20         30         40         50         60 
MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS IKHEKNSTEI 

        70         80         90        100        110        120 
QTTRPELVVS TSESIFSYYN NSTVLITGNA TGTLPGGQSH KATSTQHTVA NTTVPSDCPS 

       130        140        150        160        170        180 
EDRDLLNENV QVGLLFASKA TVQLLTNPFI GLLTNRIGYP IPMFAGFCIM FISTVMFAFS 

       190        200        210        220        230        240 
SSYAFLLIAR SLQGIGSSCS SVAGMGMLAS VYTDDEERGK PMGIALGGLA MGVLVGPPFG 

       250        260        270        280        290        300 
SVLYEFVGKT APFLVLAALV LLDGAIQLFV LQPSRVQPES QKGTPLTTLL KDPYILIAAG 

       310        320        330        340        350        360 
SICFANMGIA MLEPALPIWM METMCSRKWQ LGVAFLPASI SYLIGTNIFG ILAHKMGRWL 

       370        380        390        400        410        420 
CALLGMVIVG ISILCIPFAK NIYGLIAPNF GVGFAIGMVD SSMMPIMGYL VDLRHVSVYG 

       430        440        450        460        470        480 
SVYAIADVAF CMGYAIGPSA GGAIAKAIGF PWLMTIIGII DIAFAPLCFF LRSPPAKEEK 

       490        500        510 
MAILMDHNCP IKRKMYTQNN VQSYPIGDDE ESESD

« Hide

References

[1]"A cDNA that suppresses MPP+ toxicity encodes a vesicular amine transporter."
Liu Y., Peter D., Roghani A., Schuldiner S., Prive G.G., Eisenberg D., Brecha N., Edwards R.H.
Cell 70:539-551(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Expression cloning of a reserpine-sensitive vesicular monoamine transporter."
Erickson J.D., Eiden L.E., Hoffman B.J.
Proc. Natl. Acad. Sci. U.S.A. 89:10993-10997(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Expression and regulation of a vesicular monoamine transporter in rat stomach: a putative histamine transporter."
Dimaline R., Struthers J.
J. Physiol. (Lond.) 490:249-256(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Gastric corpus.
[4]"Phosphorylation of a vesicular monoamine transporter by casein kinase II."
Krantz D.E., Peter D., Lui Y., Edwards R.H.
J. Biol. Chem. 272:6752-6759(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-512 AND SER-514.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M97381 mRNA. Translation: AAA42190.1.
L00603 mRNA. Translation: AAA41627.1.
IPIIPI00207117.
PIRA46374.
RefSeqNP_037163.1. NM_013031.1.
UniGeneRn.9686.

3D structure databases

ProteinModelPortalQ01827.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000011983.

PTM databases

PhosphoSiteQ01827.

Proteomic databases

PaxDbQ01827.
PRIDEQ01827.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000011983; ENSRNOP00000011983; ENSRNOG00000008890.
GeneID25549.
KEGGrno:25549.

Organism-specific databases

CTD6571.
RGD3694. Slc18a2.

Phylogenomic databases

eggNOGNOG255549.
GeneTreeENSGT00390000003851.
HOGENOMHOG000018545.
HOVERGENHBG055082.
InParanoidQ01827.
KOK08155.
OrthoDBEOG4DR9C5.

Gene expression databases

GenevestigatorQ01827.
GermOnlineENSRNOG00000008890. Rattus norvegicus.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMsTIGR00880. 2_A_01_02. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBQ01827.
ChEMBLCHEMBL4828.
NextBio607093.

Entry information

Entry nameVMAT2_RAT
AccessionPrimary (citable) accession number: Q01827
Secondary accession number(s): Q9QVP1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 19, 2003
Last modified: April 3, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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