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Q01827

- VMAT2_RAT

UniProt

Q01827 - VMAT2_RAT

Protein

Synaptic vesicular amine transporter

Gene

Slc18a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (19 Sep 2003)
      Previous versions | rss
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    Functioni

    Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

    GO - Molecular functioni

    1. amine transmembrane transporter activity Source: RGD
    2. drug binding Source: RGD
    3. drug transmembrane transporter activity Source: InterPro
    4. enzyme binding Source: RGD
    5. heat shock protein binding Source: RGD
    6. serotonin transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. cellular response to ammonium ion Source: RGD
    3. cellular response to drug Source: RGD
    4. death Source: Ensembl
    5. endocytic recycling Source: RGD
    6. glucose homeostasis Source: RGD
    7. insulin secretion Source: RGD
    8. locomotory behavior Source: Ensembl
    9. monoamine transport Source: RGD
    10. negative regulation of neurotransmitter transport Source: RGD
    11. neurotransmitter transport Source: RGD
    12. post-embryonic development Source: Ensembl
    13. response to amphetamine Source: RGD
    14. response to cocaine Source: RGD
    15. response to corticosterone Source: RGD
    16. response to drug Source: RGD
    17. response to herbicide Source: RGD
    18. response to metal ion Source: RGD
    19. response to starvation Source: RGD
    20. response to zinc ion Source: RGD
    21. serotonin transport Source: GOC
    22. synaptic vesicle amine transport Source: RGD

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptic vesicular amine transporter
    Alternative name(s):
    Monoamine transporter
    Solute carrier family 18 member 2
    Vesicular amine transporter 2
    Short name:
    VAT2
    Gene namesi
    Name:Slc18a2
    Synonyms:Svat, Vmat2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi3694. Slc18a2.

    Subcellular locationi

    GO - Cellular componenti

    1. axon terminus Source: RGD
    2. cell body Source: RGD
    3. cell projection Source: RGD
    4. cytoplasm Source: RGD
    5. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    6. dense core granule Source: RGD
    7. integral component of membrane Source: UniProtKB-KW
    8. neuronal cell body Source: RGD
    9. neuron projection Source: RGD
    10. synaptic vesicle Source: RGD
    11. synaptic vesicle membrane Source: RGD
    12. terminal bouton Source: RGD

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 515515Synaptic vesicular amine transporterPRO_0000127516Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi118 ↔ 325By similarity
    Modified residuei512 – 5121Phosphoserine; by CK21 Publication
    Modified residuei514 – 5141Phosphoserine; by CK21 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ01827.
    PRIDEiQ01827.

    PTM databases

    PhosphoSiteiQ01827.

    Expressioni

    Tissue specificityi

    Brainstem and stomach.

    Gene expression databases

    GenevestigatoriQ01827.

    Interactioni

    Subunit structurei

    Interacts with SLC6A3.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000011983.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01827.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini42 – 13089Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini152 – 1609CytoplasmicSequence Analysis
    Topological domaini182 – 1909Lumenal, vesicleSequence Analysis
    Topological domaini212 – 2209CytoplasmicSequence Analysis
    Topological domaini244 – 2496Lumenal, vesicleSequence Analysis
    Topological domaini273 – 29220CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini313 – 32917Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini354 – 3585CytoplasmicSequence Analysis
    Topological domaini380 – 39011Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini412 – 4154CytoplasmicSequence Analysis
    Topological domaini437 – 4415Lumenal, vesicleSequence Analysis
    Topological domaini464 – 51552CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei131 – 15121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei161 – 18121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei191 – 21121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei221 – 24323HelicalSequence AnalysisAdd
    BLAST
    Transmembranei250 – 27223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei293 – 31220HelicalSequence AnalysisAdd
    BLAST
    Transmembranei330 – 35324HelicalSequence AnalysisAdd
    BLAST
    Transmembranei359 – 37921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei391 – 41121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei416 – 43621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei442 – 46322HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG255549.
    GeneTreeiENSGT00390000003851.
    HOGENOMiHOG000018545.
    HOVERGENiHBG055082.
    InParanoidiQ01827.
    KOiK08155.
    OrthoDBiEOG79CXZ7.
    PhylomeDBiQ01827.
    TreeFamiTF313494.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004734. Multidrug-R.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01827-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS    50
    IKHEKNSTEI QTTRPELVVS TSESIFSYYN NSTVLITGNA TGTLPGGQSH 100
    KATSTQHTVA NTTVPSDCPS EDRDLLNENV QVGLLFASKA TVQLLTNPFI 150
    GLLTNRIGYP IPMFAGFCIM FISTVMFAFS SSYAFLLIAR SLQGIGSSCS 200
    SVAGMGMLAS VYTDDEERGK PMGIALGGLA MGVLVGPPFG SVLYEFVGKT 250
    APFLVLAALV LLDGAIQLFV LQPSRVQPES QKGTPLTTLL KDPYILIAAG 300
    SICFANMGIA MLEPALPIWM METMCSRKWQ LGVAFLPASI SYLIGTNIFG 350
    ILAHKMGRWL CALLGMVIVG ISILCIPFAK NIYGLIAPNF GVGFAIGMVD 400
    SSMMPIMGYL VDLRHVSVYG SVYAIADVAF CMGYAIGPSA GGAIAKAIGF 450
    PWLMTIIGII DIAFAPLCFF LRSPPAKEEK MAILMDHNCP IKRKMYTQNN 500
    VQSYPIGDDE ESESD 515
    Length:515
    Mass (Da):55,690
    Last modified:September 19, 2003 - v2
    Checksum:i627A904C1D35D552
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti220 – 2212KP → NA in AAA41627. (PubMed:1438304)Curated
    Sequence conflicti397 – 3971G → F in AAA42190. (PubMed:1505023)Curated
    Sequence conflicti493 – 4931R → T in AAA41627. (PubMed:1438304)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97381 mRNA. Translation: AAA42190.1.
    L00603 mRNA. Translation: AAA41627.1.
    PIRiA46374.
    RefSeqiNP_037163.1. NM_013031.1.
    XP_006231726.1. XM_006231664.1.
    XP_006231727.1. XM_006231665.1.
    UniGeneiRn.9686.

    Genome annotation databases

    EnsembliENSRNOT00000011983; ENSRNOP00000011983; ENSRNOG00000008890.
    GeneIDi25549.
    KEGGirno:25549.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97381 mRNA. Translation: AAA42190.1 .
    L00603 mRNA. Translation: AAA41627.1 .
    PIRi A46374.
    RefSeqi NP_037163.1. NM_013031.1.
    XP_006231726.1. XM_006231664.1.
    XP_006231727.1. XM_006231665.1.
    UniGenei Rn.9686.

    3D structure databases

    ProteinModelPortali Q01827.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000011983.

    Chemistry

    BindingDBi Q01827.
    ChEMBLi CHEMBL4828.

    PTM databases

    PhosphoSitei Q01827.

    Proteomic databases

    PaxDbi Q01827.
    PRIDEi Q01827.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000011983 ; ENSRNOP00000011983 ; ENSRNOG00000008890 .
    GeneIDi 25549.
    KEGGi rno:25549.

    Organism-specific databases

    CTDi 6571.
    RGDi 3694. Slc18a2.

    Phylogenomic databases

    eggNOGi NOG255549.
    GeneTreei ENSGT00390000003851.
    HOGENOMi HOG000018545.
    HOVERGENi HBG055082.
    InParanoidi Q01827.
    KOi K08155.
    OrthoDBi EOG79CXZ7.
    PhylomeDBi Q01827.
    TreeFami TF313494.

    Miscellaneous databases

    NextBioi 607093.
    PROi Q01827.

    Gene expression databases

    Genevestigatori Q01827.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004734. Multidrug-R.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00880. 2_A_01_02. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A cDNA that suppresses MPP+ toxicity encodes a vesicular amine transporter."
      Liu Y., Peter D., Roghani A., Schuldiner S., Prive G.G., Eisenberg D., Brecha N., Edwards R.H.
      Cell 70:539-551(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Expression cloning of a reserpine-sensitive vesicular monoamine transporter."
      Erickson J.D., Eiden L.E., Hoffman B.J.
      Proc. Natl. Acad. Sci. U.S.A. 89:10993-10997(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Expression and regulation of a vesicular monoamine transporter in rat stomach: a putative histamine transporter."
      Dimaline R., Struthers J.
      J. Physiol. (Lond.) 490:249-256(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Gastric corpus.
    4. "Phosphorylation of a vesicular monoamine transporter by casein kinase II."
      Krantz D.E., Peter D., Lui Y., Edwards R.H.
      J. Biol. Chem. 272:6752-6759(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-512 AND SER-514.

    Entry informationi

    Entry nameiVMAT2_RAT
    AccessioniPrimary (citable) accession number: Q01827
    Secondary accession number(s): Q9QVP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: September 19, 2003
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3