Q01819 (CONN_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Connectin

Gene names

Name:	Con
ORF Names:	CG7503

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	682 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cell adhesion protein involved in target recognition during neuromuscular development. Mediates homophilic cellular adhesion.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential.
Tissue specificity	Predominantly expressed in abdominal and thoracic segment muscle and motorneuron cells.
Developmental stage	Embryo.
Sequence similarities	Contains 11 LRR (leucine-rich) repeats. Contains 1 LRRCT domain.
Sequence caution	The sequence AAF47952.3 differs from that shown. Reason: Erroneous initiation. The sequence AAM50172.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Leucine-rich repeat Repeat Signal
Molecular function	Developmental protein
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axonal fasciculation Traceable author statement. Source: FlyBase homophilic cell adhesion Traceable author statement. Source: FlyBase synaptic target attraction Traceable author statement. Source: FlyBase
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 658	634	Connectin		PRO_0000020979
	Propeptide	659 – 682	24	Removed in mature form Potential		PRO_0000020980
Regions
	Repeat	149 – 172	24	LRR 1
	Repeat	173 – 196	24	LRR 2
	Repeat	199 – 220	22	LRR 3
	Repeat	223 – 244	22	LRR 4
	Repeat	247 – 268	22	LRR 5
	Repeat	271 – 292	22	LRR 6
	Repeat	295 – 316	22	LRR 7
	Repeat	319 – 342	24	LRR 8
	Repeat	343 – 364	22	LRR 9
	Repeat	367 – 388	22	LRR 10
	Repeat	389 – 404	16	LRR 11
	Domain	405 – 462	58	LRRCT
Amino acid modifications
	Lipidation	658	1	GPI-anchor amidated alanine Potential
Experimental info
	Sequence conflict	631	1	E → G in CAA48652. Ref.2
	Sequence conflict	674 – 677	4	VALM → QVAL in AAA28424. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01819 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: E7F26353438ED06F	FASTA	682	75,995
	10 20 30 40 50 60 MATLADSAIC FLLLSLLLIG ACLVTPTEGR AKDDRRTRGR GSSSGVLSSS SSSSNNMNNG 70 80 90 100 110 120 YYSGSSSTAG SSSGYVFTSS SAVNSGSTGY SGPMDSTGFC TRRRDMKLMC YCTPDENHVP 130 140 150 160 170 180 VQKAECWVFS EGLHQNDTTW TRFYQQKRLR ELKFVIQNNA RLDYIPTMII EPLKNLSSIV 190 200 210 220 230 240 IEYSQVEIVK SYAFANLPFL ERIILNNNHI MALDQDAFAN HIRLRELNLE HNQIFEMDRY 250 260 270 280 290 300 AFRNLPLCER LFLNNNNIST LHEGLFADMA RLTFLNLAHN QINVLTSEIF RGLGNLNVLK 310 320 330 340 350 360 LTRNNLNFIG DTVFAELWSL SELELDDNRI ERISERALDG LNTLKTLNLR NNLLKKIDNG 370 380 390 400 410 420 LLRGTPALLS INVQANKLET LTFYTFQPIM DNLVNSTSEL LVSDNKFICD CRLQWIFELK 430 440 450 460 470 480 NRTRHLQLRD SLEDLHCTLQ EPKLSHFVDP VPPTILDVLN IGGFTAIGSN SASMGGVGNS 490 500 510 520 530 540 VVGSSYSGLT MDDSRKHLGS RSRQALRGQR QFASSAENVV ESKMRRRRKR QEEVKEKDLA 550 560 570 580 590 600 AVAPAHKRYD YYDDNNGGMS LGHGLDLDDN LSLHKQGFYG AGSPVVGHND VDVLMTSHSA 610 620 630 640 650 660 SGDALDILTT KNAIYIKLFL LKPEMLPCHD ELSDPTELPL SRDLMDVRSN VGQDMSTAGA 670 680 NSLAQGMTII VSLVALMMIS RG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Connectin: a homophilic cell adhesion molecule expressed on a subset of muscles and the motoneurons that innervate them in Drosophila." Nose A., Mahajan V.B., Goodman C.S. Cell 70:553-567(1992) [PubMed: 1505024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Connectin, a target of homeotic gene control in Drosophila." Gould A.P., White R.A.H. Development 116:1163-1174(1992) [PubMed: 1363542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Oregon-R.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 466-682. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96647 mRNA. Translation: AAA28424.1. X68701 mRNA. Translation: CAA48652.1. AE014296 Genomic DNA. Translation: AAF47952.3. Different initiation. AY119518 mRNA. Translation: AAM50172.1. Different initiation.
PIR	A43318. A49121.
RefSeq	NP_523930.3. NM_079206.3.
UniGene	Dm.2557.
3D structure databases
ProteinModelPortal	Q01819.
SMR	Q01819. Positions 104-446.
ModBase	Search...
Protein-protein interaction databases
STRING	Q01819.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	38590.
KEGG	dme:Dmel_CG7503.
UCSC	CG7503-RA. d. melanogaster.
Organism-specific databases
CTD	38590.
FlyBase	FBgn0005775. Con.
Phylogenomic databases
eggNOG	inNOG09238.
InParanoid	Q01819.
OrthoDB	EOG49KD5V.
Gene expression databases
Bgee	Q01819.
GermOnline	CG7503. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000483. Cys-rich_flank_reg_C. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. [Graphical view]
SMART	SM00369. LRR_TYP. 2 hits. SM00082. LRRCT. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 8 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	809410.

Entry information

Entry name

CONN_DROME

Accession

Primary (citable) accession number: Q01819
Secondary accession number(s): Q8MRN1, Q9VZ74

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	May 10, 2004
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project