Reviewed, UniProtKB/Swiss-Prot Q01819 (CONN_DROME)

Names and origin

Protein names

Recommended name:
    Connectin

Gene names

Name:	Con
ORF Names:	CG7503

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	682 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cell adhesion protein involved in target recognition during neuromuscular development. Mediates homophilic cellular adhesion.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential.
Tissue specificity	Predominantly expressed in abdominal and thoracic segment muscle and motorneuron cells.
Developmental stage	Embryo.
Sequence similarities	Contains 10 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Leucine-rich repeat Repeat Signal
Molecular function	Developmental protein
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	axonal fasciculation Traceable author statement. Source: FlyBase homophilic cell adhesion Traceable author statement. Source: FlyBase synaptic target attraction Traceable author statement. Source: FlyBase
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: FlyBase
Molecular function	protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 658	634	Connectin		PRO_0000020979
	Propeptide	659 – 682	24	Removed in mature form Potential		PRO_0000020980
Regions
	Repeat	149 – 172	24	LRR 1
	Repeat	173 – 196	24	LRR 2
	Repeat	197 – 220	24	LRR 3
	Repeat	222 – 244	23	LRR 4
	Repeat	245 – 268	24	LRR 5
	Repeat	269 – 292	24	LRR 6
	Repeat	294 – 316	23	LRR 7
	Repeat	317 – 340	24	LRR 8
	Repeat	341 – 364	24	LRR 9
	Repeat	366 – 388	23	LRR 10
Amino acid modifications
	Lipidation	658	1	GPI-anchor amidated alanine Potential
Experimental info
	Sequence conflict	631	1	E → G in CAA48652. Ref.2
	Sequence conflict	674 – 677	4	VALM → QVAL in AAA28424. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01819-1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: E7F26353438ED06F	FASTA	682	75,995
	10 20 30 40 50 60 MATLADSAIC FLLLSLLLIG ACLVTPTEGR AKDDRRTRGR GSSSGVLSSS SSSSNNMNNG 70 80 90 100 110 120 YYSGSSSTAG SSSGYVFTSS SAVNSGSTGY SGPMDSTGFC TRRRDMKLMC YCTPDENHVP 130 140 150 160 170 180 VQKAECWVFS EGLHQNDTTW TRFYQQKRLR ELKFVIQNNA RLDYIPTMII EPLKNLSSIV 190 200 210 220 230 240 IEYSQVEIVK SYAFANLPFL ERIILNNNHI MALDQDAFAN HIRLRELNLE HNQIFEMDRY 250 260 270 280 290 300 AFRNLPLCER LFLNNNNIST LHEGLFADMA RLTFLNLAHN QINVLTSEIF RGLGNLNVLK 310 320 330 340 350 360 LTRNNLNFIG DTVFAELWSL SELELDDNRI ERISERALDG LNTLKTLNLR NNLLKKIDNG 370 380 390 400 410 420 LLRGTPALLS INVQANKLET LTFYTFQPIM DNLVNSTSEL LVSDNKFICD CRLQWIFELK 430 440 450 460 470 480 NRTRHLQLRD SLEDLHCTLQ EPKLSHFVDP VPPTILDVLN IGGFTAIGSN SASMGGVGNS 490 500 510 520 530 540 VVGSSYSGLT MDDSRKHLGS RSRQALRGQR QFASSAENVV ESKMRRRRKR QEEVKEKDLA 550 560 570 580 590 600 AVAPAHKRYD YYDDNNGGMS LGHGLDLDDN LSLHKQGFYG AGSPVVGHND VDVLMTSHSA 610 620 630 640 650 660 SGDALDILTT KNAIYIKLFL LKPEMLPCHD ELSDPTELPL SRDLMDVRSN VGQDMSTAGA 670 680 NSLAQGMTII VSLVALMMIS RG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Connectin: a homophilic cell adhesion molecule expressed on a subset of muscles and the motoneurons that innervate them in Drosophila." Nose A., Mahajan V.B., Goodman C.S. Cell 70:553-567(1992) [PubMed: 1505024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Connectin, a target of homeotic gene control in Drosophila." Gould A.P., White R.A.H. Development 116:1163-1174(1992) [PubMed: 1363542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Oregon-R.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 466-682. Strain: Berkeley. Tissue: Head.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M96647 mRNA. Translation: AAA28424.1. X68701 mRNA. Translation: CAA48652.1. AE014296 Genomic DNA. Translation: AAF47952.3. Different initiation. AY119518 mRNA. Translation: AAM50172.1. Different initiation.
PIR	A43318. A49121.
RefSeq	NP_523930.3.
UniGene	Dm.2557
3D structure databases
HSSP	HSSP built from PDB template 1XUL based on UniProtKB P01233.
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0005775. Drosophila melanogaster. [Contig view]
GeneID	38590.
KEGG	dme:Dmel_CG7503.
Organism-specific databases
FlyBase	FBgn0005775. Con.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-015255-MON.
Gene expression databases
ArrayExpress	Q01819.
GermOnline	CG7503. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000483. LRR_C. [Graphical view]
Pfam	PF00560. LRR_1. 3 hits. [Graphical view]
SMART	SM00369. LRR_TYP. 2 hits. SM00082. LRRCT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	809410.

Entry information

Entry name

CONN_DROME

Accession

Primary (citable) accession number: Q01819
Secondary accession number(s): Q8MRN1, Q9VZ74

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	May 10, 2004
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project