Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - Q01814 (AT2B2_HUMAN)

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Plasma membrane calcium-transporting ATPase 2

Gene

ATP2B2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4994-aspartylphosphate intermediate1
Metal bindingi820MagnesiumBy similarity1
Metal bindingi824MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • calcium-transporting ATPase activity Source: UniProtKB
  • calmodulin binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • PDZ domain binding Source: UniProtKB
  • protein C-terminus binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  • calcium ion transport Source: DFLAT
  • ion transmembrane transport Source: Reactome
  • neuron differentiation Source: UniProtKB
  • regulation of cardiac conduction Source: Reactome
  • regulation of cytosolic calcium ion concentration Source: DFLAT
  • sensory perception of sound Source: UniProtKB
  • transport Source: UniProtKB
Complete GO annotation...

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.8. 2681.
ReactomeiR-HSA-418359. Reduction of cytosolic Ca++ levels.
R-HSA-5578775. Ion homeostasis.
R-HSA-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.2.40. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 2 (EC:3.6.3.8)
Short name:
PMCA2
Alternative name(s):
Plasma membrane calcium ATPase isoform 2
Plasma membrane calcium pump isoform 2
Gene namesi
Name:ATP2B2
Synonyms:PMCA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:815. ATP2B2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 94CytoplasmicSequence analysisAdd BLAST94
Transmembranei95 – 115HelicalSequence analysisAdd BLAST21
Topological domaini116 – 152ExtracellularSequence analysisAdd BLAST37
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 390CytoplasmicSequence analysisAdd BLAST217
Transmembranei391 – 410HelicalSequence analysisAdd BLAST20
Topological domaini411 – 443ExtracellularSequence analysisAdd BLAST33
Transmembranei444 – 461HelicalSequence analysisAdd BLAST18
Topological domaini462 – 875CytoplasmicSequence analysisAdd BLAST414
Transmembranei876 – 895HelicalSequence analysisAdd BLAST20
Topological domaini896 – 905ExtracellularSequence analysis10
Transmembranei906 – 926HelicalSequence analysisAdd BLAST21
Topological domaini927 – 946CytoplasmicSequence analysisAdd BLAST20
Transmembranei947 – 969HelicalSequence analysisAdd BLAST23
Topological domaini970 – 987ExtracellularSequence analysisAdd BLAST18
Transmembranei988 – 1009HelicalSequence analysisAdd BLAST22
Topological domaini1010 – 1028CytoplasmicSequence analysisAdd BLAST19
Transmembranei1029 – 1050HelicalSequence analysisAdd BLAST22
Topological domaini1051 – 1060ExtracellularSequence analysis10
Transmembranei1061 – 1082HelicalSequence analysisAdd BLAST22
Topological domaini1083 – 1243CytoplasmicSequence analysisAdd BLAST161

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: GO_Central
  • plasma membrane Source: UniProtKB
  • synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi491.
MalaCardsiATP2B2.
OpenTargetsiENSG00000157087.
PharmGKBiPA25108.

Polymorphism and mutation databases

BioMutaiATP2B2.
DMDMi14286115.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462141 – 1243Plasma membrane calcium-transporting ATPase 2Add BLAST1243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei364PhosphoserineBy similarity1
Modified residuei1139Phosphothreonine; by PKCBy similarity1
Modified residuei1178PhosphoserineBy similarity1
Modified residuei1188PhosphothreonineBy similarity1
Modified residuei1201Phosphoserine; by PKABy similarity1
Modified residuei1211PhosphoserineCombined sources1
Isoform ZA (identifier: Q01814-4)
Modified residuei1120PhosphoserineCombined sources1
Modified residuei1132PhosphoserineCombined sources1
Isoform XA (identifier: Q01814-7)
Modified residuei1134PhosphoserineCombined sources1
Modified residuei1146PhosphoserineCombined sources1
Isoform YA (identifier: Q01814-3)
Modified residuei1151PhosphoserineCombined sources1
Modified residuei1163PhosphoserineCombined sources1
Isoform WA (identifier: Q01814-2)
Modified residuei1165PhosphoserineCombined sources1
Modified residuei1177PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01814.
PaxDbiQ01814.
PeptideAtlasiQ01814.
PRIDEiQ01814.

PTM databases

iPTMnetiQ01814.
PhosphoSitePlusiQ01814.
SwissPalmiQ01814.

Expressioni

Tissue specificityi

Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.

Gene expression databases

BgeeiENSG00000157087.
CleanExiHS_ATP2B2.
ExpressionAtlasiQ01814. baseline and differential.
GenevisibleiQ01814. HS.

Organism-specific databases

HPAiCAB005606.

Interactioni

Subunit structurei

Interacts with PDZD11.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB
  • PDZ domain binding Source: UniProtKB
  • protein C-terminus binding Source: UniProtKB
Complete GO annotation...

Protein-protein interaction databases

BioGridi106981. 118 interactors.
IntActiQ01814. 9 interactors.
MINTiMINT-470839.
STRINGi9606.ENSP00000324172.

Structurei

3D structure databases

ProteinModelPortaliQ01814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1123 – 1140Calmodulin-binding subdomain ABy similarityAdd BLAST18
Regioni1141 – 1150Calmodulin-binding subdomain BBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi294 – 297Poly-Glu4

Sequence similaritiesi

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ01814.
KOiK05850.
OMAiLQLDQWM.
OrthoDBiEOG091G057D.
PhylomeDBiQ01814.
TreeFamiTF300330.

Family and domain databases

Gene3Di3.40.1110.10. 2 hits.
InterProiView protein in InterPro
IPR030322. ATP2B2.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
PANTHERiPTHR24093:SF370. PTHR24093:SF370. 1 hit.
PfamiView protein in Pfam
PF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF08282. Hydrolase_3. 1 hit.
SMARTiView protein in SMART
SM00831. Cation_ATPase_N. 1 hit.
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81653. SSF81653. 1 hit.
SSF81660. SSF81660. 1 hit.
SSF81665. SSF81665. 3 hits.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiView protein in PROSITE
PS00154. ATPASE_E1_E2. 1 hit.

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. AlignAdd to basket

Note: There is a combination of two alternative spliced domains at N-terminal site A (Z, X, Y and W) and at C-terminal site C (A and B). So far the splice sites have only been studied independently. Experimental confirmation may be lacking for some isoforms.
Isoform WB (identifier: Q01814-1) [UniParc]FASTAAdd to basket
Also known as: AIIICI

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMTNSDFY SKNQRNESSH GGEFGCTMEE LRSLMELRGT EAVVKIKETY 
        60         70         80         90        100
GDTEAICRRL KTSPVEGLPG TAPDLEKRKQ IFGQNFIPPK KPKTFLQLVW 
       110        120        130        140        150
EALQDVTLII LEIAAIISLG LSFYHPPGEG NEGCATAQGG AEDEGEAEAG 
       160        170        180        190        200
WIEGAAILLS VICVVLVTAF NDWSKEKQFR GLQSRIEQEQ KFTVVRAGQV 
       210        220        230        240        250
VQIPVAEIVV GDIAQVKYGD LLPADGLFIQ GNDLKIDESS LTGESDQVRK 
       260        270        280        290        300
SVDKDPMLLS GTHVMEGSGR MLVTAVGVNS QTGIIFTLLG AGGEEEEKKD 
       310        320        330        340        350
KKGVKKGDGL QLPAADGAAA SNAADSANAS LVNGKMQDGN VDASQSKAKQ 
       360        370        380        390        400
QDGAAAMEMQ PLKSAEGGDA DDRKKASMHK KEKSVLQGKL TKLAVQIGKA 
       410        420        430        440        450
GLVMSAITVI ILVLYFTVDT FVVNKKPWLP ECTPVYVQYF VKFFIIGVTV 
       460        470        480        490        500
LVVAVPEGLP LAVTISLAYS VKKMMKDNNL VRHLDACETM GNATAICSDK 
       510        520        530        540        550
TGTLTTNRMT VVQAYVGDVH YKEIPDPSSI NTKTMELLIN AIAINSAYTT 
       560        570        580        590        600
KILPPEKEGA LPRQVGNKTE CGLLGFVLDL KQDYEPVRSQ MPEEKLYKVY 
       610        620        630        640        650
TFNSVRKSMS TVIKLPDESF RMYSKGASEI VLKKCCKILN GAGEPRVFRP 
       660        670        680        690        700
RDRDEMVKKV IEPMACDGLR TICVAYRDFP SSPEPDWDNE NDILNELTCI 
       710        720        730        740        750
CVVGIEDPVR PEVPEAIRKC QRAGITVRMV TGDNINTARA IAIKCGIIHP 
       760        770        780        790        800
GEDFLCLEGK EFNRRIRNEK GEIEQERIDK IWPKLRVLAR SSPTDKHTLV 
       810        820        830        840        850
KGIIDSTHTE QRQVVAVTGD GTNDGPALKK ADVGFAMGIA GTDVAKEASD 
       860        870        880        890        900
IILTDDNFSS IVKAVMWGRN VYDSISKFLQ FQLTVNVVAV IVAFTGACIT 
       910        920        930        940        950
QDSPLKAVQM LWVNLIMDTF ASLALATEPP TETLLLRKPY GRNKPLISRT 
       960        970        980        990       1000
MMKNILGHAV YQLALIFTLL FVGEKMFQID SGRNAPLHSP PSEHYTIIFN 
      1010       1020       1030       1040       1050
TFVMMQLFNE INARKIHGER NVFDGIFRNP IFCTIVLGTF AIQIVIVQFG 
      1060       1070       1080       1090       1100
GKPFSCSPLQ LDQWMWCIFI GLGELVWGQV IATIPTSRLK FLKEAGRLTQ 
      1110       1120       1130       1140       1150
KEEIPEEELN EDVEEIDHAE RELRRGQILW FRGLNRIQTQ IRVVKAFRSS 
      1160       1170       1180       1190       1200
LYEGLEKPES RTSIHNFMAH PEFRIEDSQP HIPLIDDTDL EEDAALKQNS 
      1210       1220       1230       1240 
SPPSSLNKNN SAIDSGINLT TDTSKSATSS SPGSPIHSLE TSL
Length:1,243
Mass (Da):136,876
Last modified:June 1, 2001 - v2
Checksum:i7F10221B7B9AC3A2
GO
Isoform WA (identifier: Q01814-2) [UniParc]FASTAAdd to basket
Also known as: AIIICII

The sequence of this isoform differs from the canonical sequence as follows:
     1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG

Show »
Length:1,199
Mass (Da):131,618
Checksum:i63CF042CC00776B1
GO
Isoform YA (identifier: Q01814-3) [UniParc]FASTAAdd to basket
Also known as: AIICII

The sequence of this isoform differs from the canonical sequence as follows:
     334-347: Missing.
     1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG

Show »
Length:1,185
Mass (Da):130,171
Checksum:iDED471E8D9102EF2
GO
Isoform ZA (identifier: Q01814-4) [UniParc]FASTAAdd to basket
Also known as: AICII

The sequence of this isoform differs from the canonical sequence as follows:
     303-347: Missing.
     1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG

Show »
Length:1,154
Mass (Da):127,335
Checksum:iECB232AA4AC37BE8
GO
Isoform YB (identifier: Q01814-5) [UniParc]FASTAAdd to basket
Also known as: AIICI

The sequence of this isoform differs from the canonical sequence as follows:
     334-347: Missing.

Show »
Length:1,229
Mass (Da):135,430
Checksum:iA7E663E42EBDEAA9
GO
Isoform ZB (identifier: Q01814-6) [UniParc]FASTAAdd to basket
Also known as: AICI

The sequence of this isoform differs from the canonical sequence as follows:
     303-347: Missing.

Show »
Length:1,198
Mass (Da):132,594
Checksum:iEEF94C16D4C1C73E
GO
Isoform XA (identifier: Q01814-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-334: Missing.
     1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG

Show »
Length:1,168
Mass (Da):128,781
Checksum:iDF137BD7DE5AAA8E
GO
Isoform XB (identifier: Q01814-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-334: Missing.

Show »
Length:1,212
Mass (Da):134,040
Checksum:i988E5FEBD1F3A880
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92 – 94PKT → AKP in AAA36456 (PubMed:1427863).Curated3
Sequence conflicti126P → R in AAA50877 (PubMed:8428366).Curated1
Sequence conflicti126P → R in AAA51893 (PubMed:8428366).Curated1
Sequence conflicti517G → D in AAA50877 (PubMed:8428366).Curated1
Sequence conflicti517G → D in AAA51893 (PubMed:8428366).Curated1
Sequence conflicti667 – 668DG → EW in AAA36456 (PubMed:1427863).Curated2
Sequence conflicti1212A → S in AAA36456 (PubMed:1427863).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000384303 – 347Missing in isoform ZA and isoform ZB. 2 PublicationsAdd BLAST45
Alternative sequenceiVSP_040837304 – 334Missing in isoform XA and isoform XB. CuratedAdd BLAST31
Alternative sequenceiVSP_000385334 – 347Missing in isoform YA and isoform YB. CuratedAdd BLAST14
Alternative sequenceiVSP_0003861141 – 1243IRVVK…LETSL → IEVVNTFKSGASFQGALRRQ SSVTSQSQDVANLSSPSRVS LSNALSSPTSLPPAAAGQG in isoform WA, isoform XA, isoform YA and isoform ZA. CuratedAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97260 mRNA. Translation: AAA36456.1.
L20977 mRNA. Translation: AAA50877.1.
L00620 mRNA. Translation: AAA51893.1.
X63575 mRNA. Translation: CAA45131.1.
AC018839 Genomic DNA. No translation available.
AC090841 Genomic DNA. No translation available.
U15688 mRNA. Translation: AAA60985.1.
CCDSiCCDS2601.1. [Q01814-6]
CCDS33701.1. [Q01814-1]
CCDS82733.1. [Q01814-4]
PIRiS22393.
RefSeqiNP_001001331.1. NM_001001331.2. [Q01814-1]
NP_001317540.1. NM_001330611.1. [Q01814-4]
NP_001674.2. NM_001683.3. [Q01814-6]
XP_005265236.1. XM_005265179.4. [Q01814-1]
XP_006713238.1. XM_006713175.3. [Q01814-1]
XP_011532054.1. XM_011533752.2. [Q01814-1]
XP_016861970.1. XM_017006481.1. [Q01814-1]
XP_016861971.1. XM_017006482.1. [Q01814-1]
XP_016861972.1. XM_017006483.1. [Q01814-8]
XP_016861973.1. XM_017006484.1. [Q01814-8]
XP_016861975.1. XM_017006486.1. [Q01814-2]
XP_016861978.1. XM_017006489.1. [Q01814-7]
UniGeneiHs.268942.

Genome annotation databases

EnsembliENST00000352432; ENSP00000324172; ENSG00000157087. [Q01814-1]
ENST00000360273; ENSP00000353414; ENSG00000157087. [Q01814-1]
ENST00000383800; ENSP00000373311; ENSG00000157087. [Q01814-6]
ENST00000397077; ENSP00000380267; ENSG00000157087. [Q01814-6]
ENST00000460129; ENSP00000424494; ENSG00000157087. [Q01814-4]
ENST00000638646; ENSP00000492732; ENSG00000157087. [Q01814-4]
GeneIDi491.
KEGGihsa:491.
UCSCiuc003bvt.3. human. [Q01814-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiAT2B2_HUMAN
AccessioniPrimary (citable) accession number: Q01814
Secondary accession number(s): O00766, Q12994, Q16818
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 1, 2001
Last modified: July 5, 2017
This is version 185 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families