Reviewed,
UniProtKB/Swiss-Prot Q01814 (AT2B2_HUMAN)
Last modified
November 25, 2008.
Version 91.
History...
Clusters with 100%,
90%,
50% identity |
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Names and origin
| Protein names | Recommended name: Plasma membrane calcium-transporting ATPase 2 Short name=PMCA2 EC=3.6.3.8 Alternative name(s): Plasma membrane calcium ATPase isoform 2 Plasma membrane calcium pump isoform 2 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1243 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. |
| Catalytic activity | ATP + H(2)O + Ca(2+)(Cis) = ADP + phosphate + Ca(2+)(Trans). |
| Subcellular location | |
| Tissue specificity | Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle. |
| Sequence similarities | Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Dlg1 | Q62696 | 1 | EBI-1174262,EBI-389325 | From a different organism. |
| Dlg2 | Q63622 | 2 | EBI-1174262,EBI-396947 | From a different organism. |
| Dlg4 | P31016 | 1 | EBI-1174262,EBI-375655 | From a different organism. |
| SLC9A3R2 | Q8SQG9 | 2 | EBI-1174262,EBI-1174758 | From a different organism. |
Alternative products
| This entry describes 6 isoforms produced by alternative splicing. [Align] [Select] Notes: There is a combination of two alternative spliced domains at N-terminal site A (W, X and Z) and at C-terminal site C (A and B). So far the splice sites have only been studied independently. Experimental confirmation may be lacking for some isoforms. | ||||||
| Isoform WB (identifier: Q01814-1) Also known as: AIIICI; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform WA (identifier: Q01814-2) Also known as: AIIICII; The sequence of this isoform differs from the canonical sequence as follows: 1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG | ||||||
| Isoform XA (identifier: Q01814-3) Also known as: AIICII; The sequence of this isoform differs from the canonical sequence as follows: 334-347: Missing. 1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG | ||||||
| Isoform ZA (identifier: Q01814-4) Also known as: AICII; The sequence of this isoform differs from the canonical sequence as follows: 303-347: Missing. 1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG | ||||||
| Isoform XB (identifier: Q01814-5) Also known as: AIICI; The sequence of this isoform differs from the canonical sequence as follows: 334-347: Missing. | ||||||
| Isoform ZB (identifier: Q01814-6) Also known as: AICI; The sequence of this isoform differs from the canonical sequence as follows: 303-347: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1243 | 1243 | Plasma membrane calcium-transporting ATPase 2 | PRO_0000046214 | |||||
Regions | |||||||||
| Topological domain | 1 – 94 | 94 | Cytoplasmic Potential | ||||||
| Transmembrane | 95 – 115 | 21 | Potential | ||||||
| Topological domain | 116 – 152 | 37 | Extracellular Potential | ||||||
| Transmembrane | 153 – 173 | 21 | Potential | ||||||
| Topological domain | 174 – 390 | 217 | Cytoplasmic Potential | ||||||
| Transmembrane | 391 – 410 | 20 | Potential | ||||||
| Topological domain | 411 – 443 | 33 | Extracellular Potential | ||||||
| Transmembrane | 444 – 461 | 18 | Potential | ||||||
| Topological domain | 462 – 875 | 414 | Cytoplasmic Potential | ||||||
| Transmembrane | 876 – 895 | 20 | Potential | ||||||
| Topological domain | 896 – 905 | 10 | Extracellular Potential | ||||||
| Transmembrane | 906 – 926 | 21 | Potential | ||||||
| Topological domain | 927 – 946 | 20 | Cytoplasmic Potential | ||||||
| Transmembrane | 947 – 969 | 23 | Potential | ||||||
| Topological domain | 970 – 987 | 18 | Extracellular Potential | ||||||
| Transmembrane | 988 – 1009 | 22 | Potential | ||||||
| Topological domain | 1010 – 1028 | 19 | Cytoplasmic Potential | ||||||
| Transmembrane | 1029 – 1050 | 22 | Potential | ||||||
| Topological domain | 1051 – 1060 | 10 | Extracellular Potential | ||||||
| Transmembrane | 1061 – 1082 | 22 | Potential | ||||||
| Topological domain | 1083 – 1243 | 161 | Cytoplasmic Potential | ||||||
| Region | 1123 – 1140 | 18 | Calmodulin-binding subdomain A By similarity | ||||||
| Region | 1141 – 1150 | 10 | Calmodulin-binding subdomain B By similarity | ||||||
| Compositional bias | 294 – 297 | 4 | Poly-Glu | ||||||
Sites | |||||||||
| Active site | 499 | 1 | 4-aspartylphosphate intermediate | ||||||
| Metal binding | 820 | 1 | Magnesium By similarity | ||||||
| Metal binding | 824 | 1 | Magnesium By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1139 | 1 | Phosphothreonine; by PKC By similarity | ||||||
| Modified residue | 1201 | 1 | Phosphoserine; by PKA By similarity | ||||||
| Modified residue | 1231 | 1 | Phosphoserine | ||||||
| Modified residue | 1234 | 1 | Phosphoserine | ||||||
| Modified residue | 1238 | 1 | Phosphoserine | ||||||
| Modified residue | 1241 | 1 | Phosphothreonine | ||||||
| Modified residue | 1242 | 1 | Phosphoserine | ||||||
Natural variations | |||||||||
| Alternative sequence | 303 – 347 | 45 | Missing in isoform ZA and isoform ZB. | VSP_000384 | |||||
| Alternative sequence | 334 – 347 | 14 | Missing in isoform XA and isoform XB. | VSP_000385 | |||||
| Alternative sequence | 1141 – 1243 | 103 | IRVVK…LETSL → IEVVNTFKSGASFQGALRRQ SSVTSQSQDVANLSSPSRVS LSNALSSPTSLPPAAAGQG in isoform WA, isoform XA and isoform ZA. | VSP_000386 | |||||
Experimental info | |||||||||
| Sequence conflict | 92 – 94 | 3 | PKT → AKP in AAA36456. Ref.1 | ||||||
| Sequence conflict | 126 | 1 | P → R in AAA50877 and AAA51893. Ref.2 | ||||||
| Sequence conflict | 517 | 1 | G → D in AAA50877 and AAA51893. Ref.2 | ||||||
| Sequence conflict | 667 – 668 | 2 | DG → EW in AAA36456. Ref.1 | ||||||
| Sequence conflict | 1212 | 1 | A → S in AAA36456. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Determination of the nucleotide sequence and chromosomal localization of the ATP2B2 gene encoding human Ca(2+)-pumping ATPase isoform PMCA2." Brandt P., Ibrahim E., Bruns G.A.P., Neve R.L. Genomics 14:484-487(1992) [PubMed: 1427863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ZB). Tissue: Brain. |
| [2] | "von Hippel-Lindau syndrome: cloning and identification of the plasma membrane Ca(++)-transporting ATPase isoform 2 gene that resides in the von Hippel-Lindau gene region." Latif F., Duh F.-M., Gnarra J., Tory K., Kuzmin I., Yao M., Stackhouse T., Modi W., Geil L., Schmidt L., Li H., Orcutt M.L., Maher E., Richards F., Phipps M., Ferguson-Smith M., le Paslier D., Linehan W.M., Zbar B., Lerman M.I. Cancer Res. 53:861-867(1993) [PubMed: 8428366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ZB), ALTERNATIVE SPLICING (ISOFORM W). |
| [3] | "Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region." Heim R., Hug M., Iwata T., Strehler E.E., Carafoli E. Eur. J. Biochem. 205:333-340(1992) [PubMed: 1313367] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE (ISOFORMS WB; X AND Z). Tissue: Brain and Skeletal muscle. |
| [4] | "Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes." Stauffer T.P., Hilfiker H., Carafoli E., Strehler E.E. J. Biol. Chem. 268:25993-26003(1993) [ |

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