Reviewed,
UniProtKB/Swiss-Prot Q01814 (AT2B2_HUMAN)
Last modified
February 9, 2010.
Version 108.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Plasma membrane calcium-transporting ATPase 2 Short name=PMCA2 EC=3.6.3.8 Alternative name(s): Plasma membrane calcium ATPase isoform 2 Plasma membrane calcium pump isoform 2 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1243 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. |
| Catalytic activity | ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans). |
| Subcellular location | |
| Tissue specificity | Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle. |
| Sequence similarities | Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Dlg1 | Q62696 | 1 | EBI-1174262,EBI-389325 | From a different organism. |
| Dlg2 | Q63622 | 2 | EBI-1174262,EBI-396947 | From a different organism. |
| Dlg4 | P31016 | 1 | EBI-1174262,EBI-375655 | From a different organism. |
| SLC9A3R2 | Q8SQG9 | 2 | EBI-1174262,EBI-1174758 | From a different organism. |
Alternative products
| This entry describes 6 isoforms produced by alternative splicing. [Align] [Select] Note: There is a combination of two alternative spliced domains at N-terminal site A (W, X and Z) and at C-terminal site C (A and B). So far the splice sites have only been studied independently. Experimental confirmation may be lacking for some isoforms. | ||||||
| Isoform WB (identifier: Q01814-1) Also known as: AIIICI; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform WA (identifier: Q01814-2) Also known as: AIIICII; The sequence of this isoform differs from the canonical sequence as follows: 1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG | ||||||
| Isoform XA (identifier: Q01814-3) Also known as: AIICII; The sequence of this isoform differs from the canonical sequence as follows: 334-347: Missing. 1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG | ||||||
| Isoform ZA (identifier: Q01814-4) Also known as: AICII; The sequence of this isoform differs from the canonical sequence as follows: 303-347: Missing. 1141-1243: IRVVKAFRSS...SPIHSLETSL → IEVVNTFKSG...SLPPAAAGQG | ||||||
| Isoform XB (identifier: Q01814-5) Also known as: AIICI; The sequence of this isoform differs from the canonical sequence as follows: 334-347: Missing. | ||||||
| Isoform ZB (identifier: Q01814-6) Also known as: AICI; The sequence of this isoform differs from the canonical sequence as follows: 303-347: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1243 | 1243 | Plasma membrane calcium-transporting ATPase 2 | PRO_0000046214 | |||||
Regions | |||||||||
| Topological domain | 1 – 94 | 94 | Cytoplasmic Potential | ||||||
| Transmembrane | 95 – 115 | 21 | Potential | ||||||
| Topological domain | 116 – 152 | 37 | Extracellular Potential | ||||||
| Transmembrane | 153 – 173 | 21 | Potential | ||||||
| Topological domain | 174 – 390 | 217 | Cytoplasmic Potential | ||||||
| Transmembrane | 391 – 410 | 20 | Potential | ||||||
| Topological domain | 411 – 443 | 33 | Extracellular Potential | ||||||
| Transmembrane | 444 – 461 | 18 | Potential | ||||||
| Topological domain | 462 – 875 | 414 | Cytoplasmic Potential | ||||||
| Transmembrane | 876 – 895 | 20 | Potential | ||||||
| Topological domain | 896 – 905 | 10 | Extracellular Potential | ||||||
| Transmembrane | 906 – 926 | 21 | Potential | ||||||
| Topological domain | 927 – 946 | 20 | Cytoplasmic Potential | ||||||
| Transmembrane | 947 – 969 | 23 | Potential | ||||||
| Topological domain | 970 – 987 | 18 | Extracellular Potential | ||||||
| Transmembrane | 988 – 1009 | 22 | Potential | ||||||
| Topological domain | 1010 – 1028 | 19 | Cytoplasmic Potential | ||||||
| Transmembrane | 1029 – 1050 | 22 | Potential | ||||||
| Topological domain | 1051 – 1060 | 10 | Extracellular Potential | ||||||
| Transmembrane | 1061 – 1082 | 22 | Potential | ||||||
| Topological domain | 1083 – 1243 | 161 | Cytoplasmic Potential | ||||||
| Region | 1123 – 1140 | 18 | Calmodulin-binding subdomain A By similarity | ||||||
| Region | 1141 – 1150 | 10 | Calmodulin-binding subdomain B By similarity | ||||||
| Compositional bias | 294 – 297 | 4 | Poly-Glu | ||||||
Sites | |||||||||
| Active site | 499 | 1 | 4-aspartylphosphate intermediate | ||||||
| Metal binding | 820 | 1 | Magnesium By similarity | ||||||
| Metal binding | 824 | 1 | Magnesium By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1139 | 1 | Phosphothreonine; by PKC By similarity | ||||||
| Modified residue | 1201 | 1 | Phosphoserine; by PKA By similarity | ||||||
| Modified residue | 1231 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 1234 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 1238 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 1241 | 1 | Phosphothreonine Ref.6 | ||||||
| Modified residue | 1242 | 1 | Phosphoserine Ref.6 Ref.8 | ||||||
Natural variations | |||||||||
| Alternative sequence | 303 – 347 | 45 | Missing in isoform ZA and isoform ZB. | VSP_000384 | |||||
| Alternative sequence | 334 – 347 | 14 | Missing in isoform XA and isoform XB. | VSP_000385 | |||||
| Alternative sequence | 1141 – 1243 | 103 | IRVVK…LETSL → IEVVNTFKSGASFQGALRRQ SSVTSQSQDVANLSSPSRVS LSNALSSPTSLPPAAAGQG in isoform WA, isoform XA and isoform ZA. | VSP_000386 | |||||
Experimental info | |||||||||
| Sequence conflict | 92 – 94 | 3 | PKT → AKP in AAA36456. Ref.1 | ||||||
| Sequence conflict | 126 | 1 | P → R in AAA50877. Ref.2 | ||||||
| Sequence conflict | 126 | 1 | P → R in AAA51893. Ref.2 | ||||||
| Sequence conflict | 517 | 1 | G → D in AAA50877. Ref.2 | ||||||
| Sequence conflict | 517 | 1 | G → D in AAA51893. Ref.2 | ||||||
| Sequence conflict | 667 – 668 | 2 | DG → EW in AAA36456. Ref.1 | ||||||
| Sequence conflict | 1212 | 1 | A → S in AAA36456. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Determination of the nucleotide sequence and chromosomal localization of the ATP2B2 gene encoding human Ca(2+)-pumping ATPase isoform PMCA2." Brandt P., Ibrahim E., Bruns G.A.P., Neve R.L. Genomics 14:484-487(1992) [PubMed: 1427863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ZB). Tissue: Brain. |
| [2] | "von Hippel-Lindau syndrome: cloning and identification of the plasma membrane Ca(++)-transporting ATPase isoform 2 gene that resides in the von Hippel-Lindau gene region." Latif F., Duh F.-M., Gnarra J., Tory K., Kuzmin I., Yao M., Stackhouse T., Modi W., Geil L., Schmidt L., Li H., Orcutt M.L., Maher E., Richards F., Phipps M., Ferguson-Smith M., le Paslier D., Linehan W.M., Zbar B., Lerman M.I. Cancer Res. 53:861-867(1993) [PubMed: 8428366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ZB), ALTERNATIVE SPLICING. |
| [3] | "Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region." Heim R., Hug M., Iwata T., Strehler E.E., Carafoli E. Eur. J. Biochem. 205:333-340(1992) [PubMed: 1313367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM WB), ALTERNATIVE SPLICING. Tissue: Brain and Skeletal muscle. |
| [4] | "Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes." Stauffer T.P., Hilfiker H., Carafoli E., Strehler E.E. J. Biol. Chem. 268:25993-26003(1993) [PubMed: 8245032] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS WA/XA/ZA). Tissue: Brain cortex. |
| [5] | Erratum Stauffer T.P., Hilfiker H., Carafoli E., Strehler E.E. J. Biol. Chem. 269:32022-32022(1994) [PubMed: 7989379] [Abstract] |
| [6] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1231; SER-1234; SER-1238; THR-1241 AND SER-1242, MASS SPECTROMETRY. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1242, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M97260 mRNA. Translation: AAA36456.1. L20977 mRNA. Translation: AAA50877.1. L00620 mRNA. Translation: AAA51893.1. X63575 mRNA. Translation: CAA45131.1. U15688 mRNA. Translation: AAA60985.1. |
| IPI | IPI00009791. IPI00219293. IPI00219294. IPI00219295. IPI00219296. IPI00219297. |
| PIR | S22393. |
| RefSeq | NP_001001331.1. NP_001674.2. |
| UniGene | Hs.268942 |
3D structure databases | |
| SMR | Q01814. Positions 52-1084. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q01814. 6 interactions. |
| STRING | Q01814. |
PTM databases | |
| PhosphoSite | Q01814. |
Proteomic databases | |
| PRIDE | Q01814. |
Genome annotation databases | |
| Ensembl | ENST00000352432; ENSP00000324172; ENSG00000157087; Homo sapiens. [Genome view] ENST00000360273; ENSP00000353414; ENSG00000157087; Homo sapiens. [Genome view] |
| GeneID | 491. |
| KEGG | hsa:491. |
| UCSC | uc003bvt.1. human. uc003bvu.1. human. |
Organism-specific databases | |
| CTD | 491. |
| GeneCards | GC03M010342. |
| H-InvDB | HIX0030804. |
| HGNC | HGNC:815. ATP2B2. |
| HPA | CAB005606. |
| MIM | 108733. gene. |
| PharmGKB | PA25108. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HBG456486. |
| HOVERGEN | Q01814. |
| InParanoid | Q01814. |
| OMA | GEKMFQI. |
| OrthoDB | EOG9KM1NM. |
| PhylomeDB | Q01814. |
Enzyme and pathway databases | |
| BRENDA | 3.6.3.8. 247. |
Gene expression databases | |
| ArrayExpress | Q01814. |
| Bgee | Q01814. |
| CleanEx | HS_ATP2B2. |
| Genevestigator | Q01814. |
| GermOnline | ENSG00000157087. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR006408. ATPase_P-typ_Ca-transp_PMCA. IPR006069. ATPase_P-typ_cation-exchng_asu. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view] |
| PANTHER | PTHR11939. ATPase_P. 1 hit. |
| Pfam | PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view] |
| PRINTS | PR00119. CATATPASE. PR00121. NAKATPASE. |
| SMART | SM00831. Cation_ATPase_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 4 hits. |
| PROSITE | PS00154. ATPASE_E1_E2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 2059. |
| SOURCE | Search... |
Entry information
| Entry name | AT2B2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q01814 Secondary accession number(s): O00766, Q12994, Q16818 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


