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Q01812 (GRIK4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate receptor ionotropic, kainate 4

Short name=GluK4
Alternative name(s):
Glutamate receptor KA-1
Short name=KA1
Gene names
Name:Grik4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length956 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > glutamate >> AMPA.

Subunit structure

Forms a heteromeric channel with GRIK1 or GRIK3 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Tissue specificity

Strong expression in hippocampal CA3 pyramidal cells. Low expression in hippocampal dentate granule cells, in layers II, V and VI of the cortex, and in cerebellar Purkinje cells. No expression in the striatum, reticular thalamus, hypothalamus or amygdaloid complex.

Developmental stage

Expressed at embryonic day 15 in brain and spinal cord. At embryonic day 19 expression accumulates in the hippocampal formation. Prominent expression in the subicular cortex at postnatal days P1, P8 and P15 but then markedly reduced in the adult.

Sequence similarities

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionIon channel
Ligand-gated ion channel
Receptor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_procession transmembrane transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

ion transport

Traceable author statement PubMed 7527545. Source: RGD

ionotropic glutamate receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to corticosteroid

Inferred from expression pattern PubMed 19180187. Source: RGD

synaptic transmission

Traceable author statement PubMed 7527545. Source: RGD

synaptic transmission, glutamatergic

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentaxon

Inferred from direct assay PubMed 15844209. Source: RGD

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dendrite

Inferred from direct assay PubMed 15844209. Source: RGD

ionotropic glutamate receptor complex

Traceable author statement PubMed 7527545. Source: RGD

kainate selective glutamate receptor complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

nucleus

Inferred from direct assay PubMed 16360275. Source: RGD

perikaryon

Inferred from direct assay PubMed 15844209. Source: RGD

postsynaptic membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

presynaptic membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

terminal bouton

Inferred from direct assay PubMed 15844209PubMed 16360275. Source: RGD

   Molecular_functionextracellular-glutamate-gated ion channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

ionotropic glutamate receptor activity

Traceable author statement PubMed 7527545. Source: RGD

kainate selective glutamate receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 956936Glutamate receptor ionotropic, kainate 4
PRO_0000011551

Regions

Topological domain21 – 545525Extracellular Potential
Transmembrane546 – 56621Helical; Potential
Topological domain567 – 62357Cytoplasmic Potential
Transmembrane624 – 64421Helical; Potential
Topological domain645 – 804160Extracellular Potential
Transmembrane805 – 82521Helical; Potential
Topological domain826 – 956131Cytoplasmic Potential
Compositional bias862 – 8654Poly-Arg

Amino acid modifications

Glycosylation1581N-linked (GlcNAc...) Potential
Glycosylation2201N-linked (GlcNAc...) Potential
Glycosylation2721N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Glycosylation3231N-linked (GlcNAc...) Potential
Glycosylation4081N-linked (GlcNAc...) Potential
Glycosylation4151N-linked (GlcNAc...) Potential
Glycosylation4791N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5391S → F in AAA17830. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q01812 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 970C9D72C8D74700

FASTA956107,223
        10         20         30         40         50         60 
MPRVSAPLVL LPAWLLMVAC SPHSLRIAAI LDDPMECSRG ERLSITLAKN RINRAPERLG 

        70         80         90        100        110        120 
KAKVEVDIFE LLRDSEYETA ETMCQILPKG VVAVLGPSSS PASSSIISNI CGEKEVPHFK 

       130        140        150        160        170        180 
VAPEEFVRFQ LQRFTTLNLH PSNTDISVAV AGILNFFNCT TACLICAKAE CLLNLEKLLR 

       190        200        210        220        230        240 
QFLISKDTLS VRMLDDTRDP TPLLKEIRDD KTATIIIHAN ASMSHTILLK AAELGMVSAY 

       250        260        270        280        290        300 
YTYIFTNLEF SLQRMDSLVD DRVNILGFSI FNQSHAFFQE FSQSLNQSWQ ENCDHVPFTG 

       310        320        330        340        350        360 
PALSSALLFD AVYAVVTAVQ ELNRSQEIGV KPLSCGSAQI WQHGTSLMNY LRMVELEGLT 

       370        380        390        400        410        420 
GHIEFNSKGQ RSNYALKILQ FTRNGFRQIG QWHVAEGLSM DSRLYASNIS DSLFNTTLVV 

       430        440        450        460        470        480 
TTILENPYLM LKGNHQDMEG NDRYEGFCVD MLKELAEILR FNYKIRLVGD GVYGVPEANG 

       490        500        510        520        530        540 
TWTGMVGELI ARKADLAVAG LTITAEREKV IDFSKPFMTL GISILYRVHM GRRPGYFSSL 

       550        560        570        580        590        600 
DPFSPGVWLF MLLAYLAVSC VLFLVARLTP YEWYSPHPCA QGRCNLLVNQ YSLGNSLWFP 

       610        620        630        640        650        660 
VGGFMQQGST IAPRALSTRC VSGVWWAFTL IIISSYTANL AAFLTVQRME VPIESVDDLA 

       670        680        690        700        710        720 
DQTAIEYGTI HGGSSMTFFQ NSRYQTYQRM WNYMYSKQPS VFVKSTEEGI ARVLNSNYAF 

       730        740        750        760        770        780 
LLESTMNEYY RQRNCNLTQI GGLLDTKGYG IGMPVGSVFR DEFDLAILQL QENNRLEILK 

       790        800        810        820        830        840 
RKWWEGGKCP KEEDHRAKGL GMENIGGIFV VLICGLIVAI FMAMLEFLWT LRHSEASEVS 

       850        860        870        880        890        900 
VCQEMMTELR SIILCQDNIH PRRRRSGGLP PQPPVLEERR PRGTATLSNG KLCGAGEPDQ 

       910        920        930        940        950 
LAQRLAQEAA LVARGCTHIR VCPECRRFQG LRARPSPARS EESLEWDKTT NSSEPE 

« Hide

References

[1]"Cloning of a putative high-affinity kainate receptor expressed predominantly in hippocampal CA3 cells."
Werner P., Voigt M., Keinaenen K., Wisden W., Seeburg P.H.
Nature 351:742-744(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]Boulter J., Pecht G.
Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59996 mRNA. Translation: CAA42615.1.
U08257 mRNA. Translation: AAA17830.1.
PIRJS0685.
RefSeqNP_036704.1. NM_012572.1.
XP_006242917.1. XM_006242855.1.
UniGeneRn.10049.

3D structure databases

ProteinModelPortalQ01812.
SMRQ01812. Positions 407-527, 652-786.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000047794.

Chemistry

BindingDBQ01812.
ChEMBLCHEMBL2094119.

Proteomic databases

PaxDbQ01812.
PRIDEQ01812.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000048347; ENSRNOP00000047794; ENSRNOG00000030910.
GeneID24406.
KEGGrno:24406.
UCSCRGD:2734. rat.

Organism-specific databases

CTD2900.
RGD2734. Grik4.

Phylogenomic databases

eggNOGNOG316680.
GeneTreeENSGT00740000114953.
HOGENOMHOG000234371.
HOVERGENHBG051839.
InParanoidQ01812.
KOK05204.
OrthoDBEOG71G9W6.
PhylomeDBQ01812.

Gene expression databases

GenevestigatorQ01812.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSPR00177. NMDARECEPTOR.
SMARTSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
ProtoNetSearch...

Other

NextBio603213.
PROQ01812.

Entry information

Entry nameGRIK4_RAT
AccessionPrimary (citable) accession number: Q01812
Secondary accession number(s): Q62642
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families