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Protein

OTU domain-containing protein 4

Gene

OTUD4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme that specifically hydrolyzes 'Lys-48'-linked polyubiquitin.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei42By similarity1
Active sitei45NucleophileBy similarity1
Active sitei148By similarity1

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • thiol-dependent ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  • protein K48-linked deubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164165-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 4 (EC:3.4.19.12)
Alternative name(s):
HIV-1-induced protein HIN-1
Gene namesi
Name:OTUD4
Synonyms:HIN1, KIAA1046
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:24949. OTUD4.

Pathology & Biotechi

Organism-specific databases

DisGeNETi54726.
OpenTargetsiENSG00000164164.
PharmGKBiPA142671216.

Polymorphism and mutation databases

DMDMi302393819.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839791 – 1114OTU domain-containing protein 4Add BLAST1114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei341PhosphoserineCombined sources1
Modified residuei439PhosphotyrosineBy similarity1
Modified residuei443PhosphoserineCombined sources1
Modified residuei460PhosphotyrosineCombined sources1
Modified residuei546PhosphoserineCombined sources1
Modified residuei893PhosphoserineCombined sources1
Modified residuei900PhosphoserineCombined sources1
Modified residuei1006PhosphoserineCombined sources1
Modified residuei1011PhosphoserineBy similarity1
Modified residuei1014PhosphoserineCombined sources1
Modified residuei1023PhosphoserineCombined sources1
Modified residuei1024PhosphoserineCombined sources1
Modified residuei1049PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ01804.
MaxQBiQ01804.
PaxDbiQ01804.
PeptideAtlasiQ01804.
PRIDEiQ01804.

PTM databases

iPTMnetiQ01804.
PhosphoSitePlusiQ01804.

Expressioni

Inductioni

By HIV-1 insertion.1 Publication

Gene expression databases

BgeeiENSG00000164164.
CleanExiHS_OTUD4.
ExpressionAtlasiQ01804. baseline and differential.
GenevisibleiQ01804. HS.

Organism-specific databases

HPAiHPA036623.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
EXOSC8Q96B263EBI-1054396,EBI-371922
RELQ048643EBI-1054396,EBI-307352
STAT1P422243EBI-1054396,EBI-1057697
TCF4P158843EBI-1054396,EBI-533224

Protein-protein interaction databases

BioGridi120112. 53 interactors.
IntActiQ01804. 34 interactors.
MINTiMINT-3024530.
STRINGi9606.ENSP00000409279.

Structurei

3D structure databases

ProteinModelPortaliQ01804.
SMRiQ01804.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 155OTUPROSITE-ProRule annotationAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 45Cys-loopBy similarity7
Regioni94 – 104Variable-loopBy similarityAdd BLAST11
Regioni143 – 148His-loopBy similarity6

Sequence similaritiesi

Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2605. Eukaryota.
ENOG410XNYW. LUCA.
GeneTreeiENSGT00530000063508.
HOVERGENiHBG082091.
InParanoidiQ01804.
KOiK13718.
OMAiGEESWKG.
OrthoDBiEOG091G00ZF.
PhylomeDBiQ01804.
TreeFamiTF326812.

Family and domain databases

InterProiIPR003323. OTU_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q01804-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAAVGVPDG GDQGGAGPRE DATPMDAYLR KLGLYRKLVA KDGSCLFRAV
60 70 80 90 100
AEQVLHSQSR HVEVRMACIH YLRENREKFE AFIEGSFEEY LKRLENPQEW
110 120 130 140 150
VGQVEISALS LMYRKDFIIY REPNVSPSQV TENNFPEKVL LCFSNGNHYD
160 170 180 190 200
IVYPIKYKES SAMCQSLLYE LLYEKVFKTD VSKIVMELDT LEVADEDNSE
210 220 230 240 250
ISDSEDDSCK SKTAAAAADV NGFKPLSGNE QLKNNGNSTS LPLSRKVLKS
260 270 280 290 300
LNPAVYRNVE YEIWLKSKQA QQKRDYSIAA GLQYEVGDKC QVRLDHNGKF
310 320 330 340 350
LNADVQGIHS ENGPVLVEEL GKKHTSKNLK APPPESWNTV SGKKMKKPST
360 370 380 390 400
SGQNFHSDVD YRGPKNPSKP IKAPSALPPR LQHPSGVRQH AFSSHSSGSQ
410 420 430 440 450
SQKFSSEHKN LSRTPSQIIR KPDRERVEDF DHTSRESNYF GLSPEERREK
460 470 480 490 500
QAIEESRLLY EIQNRDEQAF PALSSSSVNQ SASQSSNPCV QRKSSHVGDR
510 520 530 540 550
KGSRRRMDTE ERKDKDSIHG HSQLDKRPEP STLENITDDK YATVSSPSKS
560 570 580 590 600
KKLECPSPAE QKPAEHVSLS NPAPLLVSPE VHLTPAVPSL PATVPAWPSE
610 620 630 640 650
PTTFGPTGVP APIPVLSVTQ TLTTGPDSAV SQAHLTPSPV PVSIQAVNQP
660 670 680 690 700
LMPLPQTLSL YQDPLYPGFP CNEKGDRAIV PPYSLCQTGE DLPKDKNILR
710 720 730 740 750
FFFNLGVKAY SCPMWAPHSY LYPLHQAYLA ACRMYPKVPV PVYPHNPWFQ
760 770 780 790 800
EAPAAQNESD CTCTDAHFPM QTEASVNGQM PQPEIGPPTF SSPLVIPPSQ
810 820 830 840 850
VSESHGQLSY QADLESETPG QLLHADYEES LSGKNMFPQP SFGPNPFLGP
860 870 880 890 900
VPIAPPFFPH VWYGYPFQGF IENPVMRQNI VLPSDEKGEL DLSLENLDLS
910 920 930 940 950
KDCGSVSTVD EFPEARGEHV HSLPEASVSS KPDEGRTEQS SQTRKADTAL
960 970 980 990 1000
ASIPPVAEGK AHPPTQILNR ERETVPVELE PKRTIQSLKE KTEKVKDPKT
1010 1020 1030 1040 1050
AADVVSPGAN SVDSRVQRPK EESSEDENEV SNILRSGRSK QFYNQTYGSR
1060 1070 1080 1090 1100
KYKSDWGYSG RGGYQHVRSE ESWKGQPSRS RDEGYQYHRN VRGRPFRGDR
1110
RRSGMGDGHR GQHT
Note: Gene prediction based on similarity to orthologs and partial EST data.
Length:1,114
Mass (Da):124,045
Last modified:March 19, 2014 - v4
Checksum:iAD8DF5FEAB8CBE32
GO
Isoform 2 (identifier: Q01804-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.
     231-231: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,048
Mass (Da):116,953
Checksum:i5266BAE77822228E
GO
Isoform 3 (identifier: Q01804-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Show »
Length:1,049
Mass (Da):117,082
Checksum:i1DCACF002992C917
GO
Isoform 4 (identifier: Q01804-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.
     211-1114: Missing.

Note: Predicted from a chimeric transcript isolated from HIV-1-infected cells. The premature stop may be due to intron retention.
Show »
Length:145
Mass (Da):17,036
Checksum:iBEF79BFC3DB34EF1
GO

Sequence cautioni

The sequence AAH57242 differs from that shown. Sequence of unknown origin in the N-terminal part.Curated
The sequence BAA82998 differs from that shown. Intron retention.Curated
The sequence BAB71229 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti809S → P in BAB71229 (PubMed:14702039).Curated1
Sequence conflicti915A → S in CAB70748 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_029377194A → G.1 PublicationCorresponds to variant rs36225458dbSNPEnsembl.1
Natural variantiVAR_038848216A → T.1 PublicationCorresponds to variant rs36225838dbSNPEnsembl.1
Natural variantiVAR_070050398G → V Could be associated with cerebellar ataxia and hypogonadotropic hypogonadism. 1 PublicationCorresponds to variant rs148857745dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0388301 – 65Missing in isoform 2, isoform 3 and isoform 4. 3 PublicationsAdd BLAST65
Alternative sequenceiVSP_021671211 – 1114Missing in isoform 4. 1 PublicationAdd BLAST904
Alternative sequenceiVSP_053825231Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056597 mRNA. Translation: BAB71229.1. Different initiation.
AK302581 mRNA. Translation: BAG63836.1.
AC096757 Genomic DNA. No translation available.
CH471056 Genomic DNA. Translation: EAX05045.1.
CH471056 Genomic DNA. Translation: EAX05046.1.
X68242 mRNA. Translation: CAA48313.1.
DQ427109 Genomic DNA. Translation: ABD72605.1.
BC057242 mRNA. Translation: AAH57242.2. Sequence problems.
BC118572 mRNA. Translation: AAI18573.1.
BC118653 mRNA. Translation: AAI18654.1.
AB028969 mRNA. Translation: BAA82998.2. Sequence problems.
AL137460 mRNA. Translation: CAB70748.1.
CCDSiCCDS3764.1. [Q01804-4]
CCDS47139.1. [Q01804-3]
PIRiS30247.
T46403.
RefSeqiNP_001096123.1. NM_001102653.1. [Q01804-3]
NP_059963.1. NM_017493.6. [Q01804-4]
XP_005263136.1. XM_005263079.4. [Q01804-1]
UniGeneiHs.270851.

Genome annotation databases

EnsembliENST00000447906; ENSP00000395487; ENSG00000164164. [Q01804-1]
ENST00000454497; ENSP00000409279; ENSG00000164164. [Q01804-3]
ENST00000509620; ENSP00000424192; ENSG00000164164. [Q01804-4]
GeneIDi54726.
KEGGihsa:54726.
UCSCiuc003ika.5. human. [Q01804-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056597 mRNA. Translation: BAB71229.1. Different initiation.
AK302581 mRNA. Translation: BAG63836.1.
AC096757 Genomic DNA. No translation available.
CH471056 Genomic DNA. Translation: EAX05045.1.
CH471056 Genomic DNA. Translation: EAX05046.1.
X68242 mRNA. Translation: CAA48313.1.
DQ427109 Genomic DNA. Translation: ABD72605.1.
BC057242 mRNA. Translation: AAH57242.2. Sequence problems.
BC118572 mRNA. Translation: AAI18573.1.
BC118653 mRNA. Translation: AAI18654.1.
AB028969 mRNA. Translation: BAA82998.2. Sequence problems.
AL137460 mRNA. Translation: CAB70748.1.
CCDSiCCDS3764.1. [Q01804-4]
CCDS47139.1. [Q01804-3]
PIRiS30247.
T46403.
RefSeqiNP_001096123.1. NM_001102653.1. [Q01804-3]
NP_059963.1. NM_017493.6. [Q01804-4]
XP_005263136.1. XM_005263079.4. [Q01804-1]
UniGeneiHs.270851.

3D structure databases

ProteinModelPortaliQ01804.
SMRiQ01804.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120112. 53 interactors.
IntActiQ01804. 34 interactors.
MINTiMINT-3024530.
STRINGi9606.ENSP00000409279.

PTM databases

iPTMnetiQ01804.
PhosphoSitePlusiQ01804.

Polymorphism and mutation databases

DMDMi302393819.

Proteomic databases

EPDiQ01804.
MaxQBiQ01804.
PaxDbiQ01804.
PeptideAtlasiQ01804.
PRIDEiQ01804.

Protocols and materials databases

DNASUi54726.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000447906; ENSP00000395487; ENSG00000164164. [Q01804-1]
ENST00000454497; ENSP00000409279; ENSG00000164164. [Q01804-3]
ENST00000509620; ENSP00000424192; ENSG00000164164. [Q01804-4]
GeneIDi54726.
KEGGihsa:54726.
UCSCiuc003ika.5. human. [Q01804-1]

Organism-specific databases

CTDi54726.
DisGeNETi54726.
GeneCardsiOTUD4.
HGNCiHGNC:24949. OTUD4.
HPAiHPA036623.
MIMi611744. gene.
neXtProtiNX_Q01804.
OpenTargetsiENSG00000164164.
PharmGKBiPA142671216.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2605. Eukaryota.
ENOG410XNYW. LUCA.
GeneTreeiENSGT00530000063508.
HOVERGENiHBG082091.
InParanoidiQ01804.
KOiK13718.
OMAiGEESWKG.
OrthoDBiEOG091G00ZF.
PhylomeDBiQ01804.
TreeFamiTF326812.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164165-MONOMER.

Miscellaneous databases

ChiTaRSiOTUD4. human.
GeneWikiiOTUD4.
GenomeRNAii54726.
PROiQ01804.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164164.
CleanExiHS_OTUD4.
ExpressionAtlasiQ01804. baseline and differential.
GenevisibleiQ01804. HS.

Family and domain databases

InterProiIPR003323. OTU_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTUD4_HUMAN
AccessioniPrimary (citable) accession number: Q01804
Secondary accession number(s): B4DYS4
, Q147U2, Q1ZYK1, Q6PG39, Q96MQ5, Q9NT94, Q9UPV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: March 19, 2014
Last modified: November 2, 2016
This is version 133 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.