Reviewed, UniProtKB/Swiss-Prot Q01804 (OTUD4_HUMAN)

Names and origin

Protein names

Recommended name:
    OTU domain-containing protein 4
Alternative name(s):
    HIV-1-induced protein HIN-1

Gene names

Name:	OTUD4
Synonyms:	HIN1, KIAA1046

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1114 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Induction	By HIV-1 insertion. Ref.2
Sequence similarities	Contains 1 OTU domain.
Sequence caution	The sequence ABD72605.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAA82998.2 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention. The sequence CAA48313.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Originates from a chimeric transcript in HIV-1-infected cells. The premature stop may be due to intron retention.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG12	O94817	1	EBI-1054396,EBI-746742
BRF2	Q9HAW0	1	EBI-1054396,EBI-1055224
CLEC3B	P05452	1	EBI-1054396,EBI-1047626
ILK	Q13418	1	EBI-1054396,EBI-747644
RUNX1T1	Q06455	1	EBI-1054396,EBI-743342

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q01804-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q01804-2)

The sequence of this isoform differs from the canonical sequence as follows:
     211-1114: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1114	1114	OTU domain-containing protein 4		PRO_0000083979
Regions
	Domain	34 – 155	122	OTU
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.1
	Modified residue	439	1	Phosphotyrosine Ref.8
	Modified residue	443	1	Phosphoserine Ref.11
	Modified residue	941	1	Phosphoserine Ref.10
	Modified residue	1006	1	Phosphoserine Ref.11
	Modified residue	1023	1	Phosphoserine Ref.11 Ref.9
	Modified residue	1024	1	Phosphoserine Ref.11 Ref.9
Natural variations
	Alternative sequence	211 – 1114	904	Missing in isoform 2.		VSP_021671
	Natural variant	194	1	A → G: dbSNP rs36225458. Ref.3		VAR_029377
	Natural variant	216	1	A → T Ref.3		VAR_038848
Experimental info
	Sequence conflict	809	1	S → P in BAB71229. Ref.5
	Sequence conflict	915	1	A → S in CAB70748. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: AD8DF5FEAB8CBE32	FASTA	1,114	124,045
	10 20 30 40 50 60 MEAAVGVPDG GDQGGAGPRE DATPMDAYLR KLGLYRKLVA KDGSCLFRAV AEQVLHSQSR 70 80 90 100 110 120 HVEVRMACIH YLRENREKFE AFIEGSFEEY LKRLENPQEW VGQVEISALS LMYRKDFIIY 130 140 150 160 170 180 REPNVSPSQV TENNFPEKVL LCFSNGNHYD IVYPIKYKES SAMCQSLLYE LLYEKVFKTD 190 200 210 220 230 240 VSKIVMELDT LEVADEDNSE ISDSEDDSCK SKTAAAAADV NGFKPLSGNE QLKNNGNSTS 250 260 270 280 290 300 LPLSRKVLKS LNPAVYRNVE YEIWLKSKQA QQKRDYSIAA GLQYEVGDKC QVRLDHNGKF 310 320 330 340 350 360 LNADVQGIHS ENGPVLVEEL GKKHTSKNLK APPPESWNTV SGKKMKKPST SGQNFHSDVD 370 380 390 400 410 420 YRGPKNPSKP IKAPSALPPR LQHPSGVRQH AFSSHSSGSQ SQKFSSEHKN LSRTPSQIIR 430 440 450 460 470 480 KPDRERVEDF DHTSRESNYF GLSPEERREK QAIEESRLLY EIQNRDEQAF PALSSSSVNQ 490 500 510 520 530 540 SASQSSNPCV QRKSSHVGDR KGSRRRMDTE ERKDKDSIHG HSQLDKRPEP STLENITDDK 550 560 570 580 590 600 YATVSSPSKS KKLECPSPAE QKPAEHVSLS NPAPLLVSPE VHLTPAVPSL PATVPAWPSE 610 620 630 640 650 660 PTTFGPTGVP APIPVLSVTQ TLTTGPDSAV SQAHLTPSPV PVSIQAVNQP LMPLPQTLSL 670 680 690 700 710 720 YQDPLYPGFP CNEKGDRAIV PPYSLCQTGE DLPKDKNILR FFFNLGVKAY SCPMWAPHSY 730 740 750 760 770 780 LYPLHQAYLA ACRMYPKVPV PVYPHNPWFQ EAPAAQNESD CTCTDAHFPM QTEASVNGQM 790 800 810 820 830 840 PQPEIGPPTF SSPLVIPPSQ VSESHGQLSY QADLESETPG QLLHADYEES LSGKNMFPQP 850 860 870 880 890 900 SFGPNPFLGP VPIAPPFFPH VWYGYPFQGF IENPVMRQNI VLPSDEKGEL DLSLENLDLS 910 920 930 940 950 960 KDCGSVSTVD EFPEARGEHV HSLPEASVSS KPDEGRTEQS SQTRKADTAL ASIPPVAEGK 970 980 990 1000 1010 1020 AHPPTQILNR ERETVPVELE PKRTIQSLKE KTEKVKDPKT AADVVSPGAN SVDSRVQRPK 1030 1040 1050 1060 1070 1080 EESSEDENEV SNILRSGRSK QFYNQTYGSR KYKSDWGYSG RGGYQHVRSE ESWKGQPSRS 1090 1100 1110 RDEGYQYHRN VRGRPFRGDR RRSGMGDGHR GQHT
	Isoform 2. Checksum: AE1B7CAC54E8D072	FASTA	210	23,999
	10 20 30 40 50 60 MEAAVGVPDG GDQGGAGPRE DATPMDAYLR KLGLYRKLVA KDGSCLFRAV AEQVLHSQSR 70 80 90 100 110 120 HVEVRMACIH YLRENREKFE AFIEGSFEEY LKRLENPQEW VGQVEISALS LMYRKDFIIY 130 140 150 160 170 180 REPNVSPSQV TENNFPEKVL LCFSNGNHYD IVYPIKYKES SAMCQSLLYE LLYEKVFKTD 190 200 210 VSKIVMELDT LEVADEDNSE ISDSEDDSCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Bienvenut W.V., Heiserich L., Boulahbel H., Gottlieb E., Matallanas D., Cooper W.N., Kolch W. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-19; 49-60; 213-145; 250-266; 414-420; 436-447; 458-465; 701-708; 917-936; 945-970 AND 1000-1015, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Colon carcinoma and Mammary carcinoma.
[2]	"HIV-1 promotor insertion revealed by selective detection of chimeric provirus-host gene transcripts." Raineri I., Senn H.-P. Nucleic Acids Res. 20:6261-6266(1992) [PubMed: 1475186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-1114 (ISOFORM 2), INDUCTION.
[3]	SeattleSNPs variation discovery resource Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 54-1114, VARIANTS GLY-194 AND THR-216.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 56-1114 (ISOFORM 1). Tissue: Brain.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 285-988 (ISOFORM 1). Tissue: Tongue.
[6]	"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:197-205(1999) [PubMed: 10470851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 709-1114. Tissue: Brain.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 815-1114. Tissue: Testis.
[8]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-439, MASS SPECTROMETRY.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1023 AND SER-1024, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-941, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-443; SER-1006; SER-1023 AND SER-1024, MASS SPECTROMETRY.
[12]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	X68242 mRNA. Translation: CAA48313.1. Sequence problems. DQ427109 Genomic DNA. Translation: ABD72605.1. Sequence problems. BC057242 mRNA. Translation: AAH57242.1. Different initiation. BC118572 mRNA. Translation: AAI18573.1. Different initiation. BC118653 mRNA. Translation: AAI18654.1. Different initiation. AK056597 mRNA. Translation: BAB71229.1. Different initiation. AB028969 mRNA. Translation: BAA82998.2. Sequence problems. AL137460 mRNA. Translation: CAB70748.1.
IPI	IPI00009780. IPI00399254.
PIR	S30247. T46403.
RefSeq	NP_001096123.1. NP_059963.1. NP_955356.1.
UniGene	Hs.270851
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q01804. 8 interactions.
Proteomic databases
PRIDE	Q01804.
Genome annotation databases
Ensembl	ENSG00000164164. Homo sapiens. [Contig view]
GeneID	54726.
KEGG	hsa:54726.
Organism-specific databases
GeneCards	GC04M146274.
HGNC	HGNC:24949. OTUD4.
MIM	611744. gene.
PharmGKB	PA142671216.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q01804.
OMA	Q01804. EVSNILR.
Gene expression databases
ArrayExpress	Q01804.
Bgee	Q01804.
CleanEx	HS_OTUD4.
GermOnline	ENSG00000164164. Homo sapiens.
Family and domain databases
InterPro	IPR003323. OTU. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	57297.
SOURCE	Search...

Entry information

Entry name

OTUD4_HUMAN

Accession

Primary (citable) accession number: Q01804
Secondary accession number(s): Q147U2 , Q1ZYK1, Q6PG39, Q96MQ5, Q9NT94, Q9UPV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 28, 2006
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)