Reviewed, UniProtKB/Swiss-Prot Q01802 (AATM_YEAST)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase, mitochondrial
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Name:	AAT1
Ordered Locus Names:	YKL106W
ORF Names:	YKL461

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate. Ref.1
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion matrix. Ref.4 Ref.6 Ref.7
Miscellaneous	In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes. Present with 2910 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro chronological cell aging Inferred from mutant phenotype. Source: SGD replicative cell aging Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 451		Aspartate aminotransferase, mitochondrial		PRO_0000001213
Amino acid modifications
	Modified residue	286	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	41	1	V → A in CAA48188. Ref.1
	Sequence conflict	414	1	Y → S in CAA48188. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01802-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 758A90434CDBAA7A	FASTA	451	51,795
	10 20 30 40 50 60 MLRTRLTNCS LWRPYYTSSL SRVPRAPPDK VLGLSEHFKK VKNVNKIDLT VGIYKDGWGK 70 80 90 100 110 120 VTTFPSVAKA QKLIESHLEL NKNLSYLPIT GSKEFQENVM KFLFKESCPQ FGPFYLAHDR 130 140 150 160 170 180 ISFVQTLSGT GALAVAAKFL ALFISRDIWI PDPSWANHKN IFQNNGFENI YRYSYYKDGQ 190 200 210 220 230 240 IDIDGWIEQL KTFAYNNQQE NNKNPPCIIL HACCHNPTGL DPTKEQWEKI IDTIYELKMV 250 260 270 280 290 300 PIVDMAYQGL ESGNLLKDAY LLRLCLNVNK YPNWSNGIFL CQSFAKNMGL YGERVGSLSV 310 320 330 340 350 360 ITPATANNGK FNPLQQKNSL QQNIDSQLKK IVRGMYSSPP GYGSRVVNVV LSDFKLKQQW 370 380 390 400 410 420 FKDVDFMVQR LHHVRQEMFD RLGWPDLVNF AQQHGMFYYT RFSPKQVEIL RNNYFVYLTG 430 440 450 DGRLSLSGVN DSNVDYLCES LEAVSKMDKL A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AAT1, a gene encoding a mitochondrial aspartate aminotransferase in Saccharomyces cerevisiae." Morin P.J., Subramanian G.S., Gilmore T.D. Biochim. Biophys. Acta 1171:211-214(1992) [PubMed: 1482685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY.
[2]	"The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast." Cheret G., Pallier C., Valens M., Daignan-Fornier B., Fukuhara H., Bolotin-Fukuhara M., Sor F. Yeast 9:1259-1265(1993) [PubMed: 8109175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed: 16823961] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X68052 Genomic DNA. Translation: CAA48188.1. X71133 Genomic DNA. Translation: CAA50451.1. Z28106 Genomic DNA. Translation: CAA81946.1.
PIR	S37933.
RefSeq	NP_012816.1.
3D structure databases
HSSP	HSSP built from PDB template 7AAT based on UniProtKB P00508.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4608N.
IntAct	Q01802. 4 interactions.
STRING	Q01802.
Proteomic databases
PeptideAtlas	Q01802.
Genome annotation databases
Ensembl	YKL106W; YKL106W; YKL106W; Saccharomyces cerevisiae. [Genome view]
GeneID	853755.
GenomeReviews	Gene locus YKL106W in contig Y13137_GR.
KEGG	sce:YKL106W.
NMPDR	fig|4932.3.peg.3798.
Organism-specific databases
CYGD	YKL106w.
SGD	S000001589. AAT1.
Phylogenomic databases
HOGENOM	Q01802.
OMA	YGERTGS.
Enzyme and pathway databases
BRENDA	2.6.1.1. 250.
Gene expression databases
ArrayExpress	Q01802.
Genevestigator	Q01802.
GermOnline	YKL106W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004839. Aminotransferase_I/II. IPR000796. Asp_trans. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11879. Asp_trans. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00799. TRANSAMINASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974828.

Entry information

Entry name

AATM_YEAST

Accession

Primary (citable) accession number: Q01802

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)