Q01796 (RNS2_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribonuclease S-2 EC=3.1.27.1 Alternative name(s): S2-RNase Stylar glycoprotein 2
Organism	Solanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Self-incompatibility (SI) is the inherited ability of a flowering plant to prevent self-fertilization by discriminating between self and non-self pollen during pollination. In many species of the Solanaceae, self-incompatibility is controlled by the single, multiallelic locus S. This stylar glycoprotein is associated with expression of self-incompatibility in potato.
Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Pistil.
Sequence similarities	Belongs to the RNase T2 family.
Caution	Gln-112 is present instead of the conserved Glu which is expected to act as an active site proton donor.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease T2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 223	201	Ribonuclease S-2		PRO_0000030986
Sites
	Active site	55	1	By similarity
	Active site	116	1	By similarity
Amino acid modifications
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Disulfide bond	71 ↔ 119		By similarity
	Disulfide bond	178 ↔ 211		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01796 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 9BAFCCF15D737FDE	FASTA	223	26,075
	10 20 30 40 50 60 MAKSQLVSAL FVFFFSLSPI YGDFDYMQLV LTWPRSFCYP RGFCNRIPPN NFTIHGLWPD 70 80 90 100 110 120 KKPMRGQLQF CTSDDYIKFT PGSVLDALDH HWIQLKFERE IGIRDQPLWK DQYKKHGTCC 130 140 150 160 170 180 LPRYNQLQYF LLAMRLKEKF DLLTTLRTHG ITPGTKHTFK KIQDAIKTVT QEVPDLKCVE 190 200 210 220 NIQGVLELYE IGICFTPEAD SLFPCRQSKS CHPTENPLIL FRL

References

[1]	"Sequence variability and gene structure at the self-incompatibility locus of Solanum tuberosum." Kaufmann H., Salamini F., Thompson R.D. Mol. Gen. Genet. 226:457-466(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62727 Genomic DNA. Translation: CAA44600.1.
PIR	PQ0749. S16007.
3D structure databases
ProteinModelPortal	Q01796.
SMR	Q01796. Positions 23-222.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.90.730.10. 1 hit.
InterPro	IPR001568. RNase_T2-like. IPR018188. RNase_T2_AS. [Graphical view]
PANTHER	PTHR11240. PTHR11240. 1 hit.
Pfam	PF00445. Ribonuclease_T2. 1 hit. [Graphical view]
SUPFAM	SSF55895. RNase_T2. 1 hit.
PROSITE	PS00530. RNASE_T2_1. 1 hit. PS00531. RNASE_T2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNS2_SOLTU

Accession

Primary (citable) accession number: Q01796

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program