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Q01786

- GUN5_THEFU

UniProt

Q01786 - GUN5_THEFU

Protein

Endoglucanase E-5

Gene

celE

Organism
Thermobifida fusca (Thermomonospora fusca)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei299 – 2991Proton donorBy similarity
    Active sitei391 – 3911NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. polysaccharide binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    UniPathwayiUPA00696.

    Protein family/group databases

    CAZyiCBM2. Carbohydrate-Binding Module Family 2.
    GH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase E-5 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase E-5
    Cellulase E5
    Endo-1,4-beta-glucanase E-4
    Gene namesi
    Name:celE
    OrganismiThermobifida fusca (Thermomonospora fusca)
    Taxonomic identifieri2021 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeNocardiopsaceaeThermobifida

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3636Add
    BLAST
    Chaini37 – 466430Endoglucanase E-5PRO_0000007866Add
    BLAST

    Structurei

    Secondary structure

    1
    466
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi164 – 1685
    Beta strandi172 – 1743
    Beta strandi177 – 1793
    Beta strandi189 – 1924
    Helixi196 – 1994
    Helixi200 – 2023
    Helixi205 – 2139
    Beta strandi218 – 22710
    Helixi231 – 2333
    Helixi235 – 25016
    Turni251 – 2533
    Beta strandi255 – 2617
    Helixi268 – 2714
    Helixi272 – 28615
    Beta strandi290 – 2956
    Helixi305 – 32218
    Beta strandi328 – 3303
    Helixi333 – 3364
    Helixi340 – 3423
    Helixi347 – 3515
    Beta strandi357 – 36610
    Turni367 – 3693
    Helixi372 – 38413
    Beta strandi387 – 3959
    Helixi405 – 41814
    Beta strandi422 – 4254
    Beta strandi435 – 4373
    Helixi441 – 4444
    Helixi450 – 4523
    Helixi455 – 46511

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CKRX-ray1.77A/B161-466[»]
    2CKSX-ray1.60A/B161-466[»]
    ProteinModelPortaliQ01786.
    SMRiQ01786. Positions 161-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ01786.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 139103CBM2Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.290. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00553. CBM_2. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view]
    SMARTiSM00637. CBD_II. 1 hit.
    [Graphical view]
    SUPFAMiSSF49384. SSF49384. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS51173. CBM2. 1 hit.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01786-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKSPAARKG XPPVAVAVTA ALALLIALLS PGVAQAAGLT ATVTKESSWD    50
    NGYSASVTVR NDTSSTVSQW EVVLTLPGGT TVAQVWNAQH TSSGNSHTFT 100
    GVSWNSTIPP GGTASSGFIA SGSGEPTHCT INGAPCDEGS EPGGPGGPGT 150
    PSPDPGTQPG TGTPVERYGK VQVCGTQLCD EHGNPVQLRG MSTHGIQWFD 200
    HCLTDSSLDA LAYDWKADII RLSMYIQEDG YETNPRGFTD RMHQLIDMAT 250
    ARGLYVIVDW HILTPGDPHY NLDRAKTFFA EIAQRHASKT NVLYEIANEP 300
    NGVSWASIKS YAEEVIPVIR QRDPDSVIIV GTRGWSSLGV SEGSGPAEIA 350
    ANPVNASNIM YAFHFYAASH RDNYLNALRE ASELFPVFVT EFGTETYTGD 400
    GANDFQMADR YIDLMAERKI GWTKWNYSDD FRSGAVFQPG TCASGGPWSG 450
    SSLKASGQWV RSKLQS 466
    Length:466
    Mass (Da):49,801
    Last modified:July 15, 1999 - v2
    Checksum:i1CF0ADFBF2DEF82E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01577 Genomic DNA. Translation: AAC09379.1.
    PIRiC42360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01577 Genomic DNA. Translation: AAC09379.1 .
    PIRi C42360.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CKR X-ray 1.77 A/B 161-466 [» ]
    2CKS X-ray 1.60 A/B 161-466 [» ]
    ProteinModelPortali Q01786.
    SMRi Q01786. Positions 161-466.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM2. Carbohydrate-Binding Module Family 2.
    GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00696 .

    Miscellaneous databases

    EvolutionaryTracei Q01786.

    Family and domain databases

    Gene3Di 2.60.40.290. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00553. CBM_2. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SMARTi SM00637. CBD_II. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49384. SSF49384. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS51173. CBM2. 1 hit.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca."
      Lao G., Ghangas G.S., Jung E.D., Wilson D.B.
      J. Bacteriol. 173:3397-3407(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YX.
    2. Lao G., Ghangas G.S., Jung E.D., Wilson D.B.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Activity studies of eight purified cellulases: specificity, synergism, and binding domain effects."
      Irwin D.C., Spezio M., Walker L.P., Wilson D.B.
      Biotechnol. Bioeng. 42:1002-1013(1993)
      Cited for: PROTEIN SEQUENCE OF 137-142 AND 157-166.
      Strain: YX.

    Entry informationi

    Entry nameiGUN5_THEFU
    AccessioniPrimary (citable) accession number: Q01786
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3