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Protein

Pteridine reductase 1

Gene

PTR1

Organism
Leishmania major
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).1 Publication

Catalytic activityi

5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH.

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from biopterin.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Pteridine reductase 1 (PTR1)
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from biopterin, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei175Substrate1 Publication1
Active sitei194Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 40NADPBy similarityAdd BLAST24

GO - Molecular functioni

  • pteridine reductase activity Source: GeneDB
  • thymidylate synthase activity Source: GeneDB

GO - Biological processi

  • cellular response to oxidative stress Source: GeneDB
  • pteridine metabolic process Source: GeneDB
  • response to methotrexate Source: UniProtKB-KW
  • tetrahydrobiopterin biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processMethotrexate resistance
LigandNADP

Enzyme and pathway databases

BRENDAi1.5.1.33 2950
SABIO-RKiQ01782
UniPathwayiUPA00849; UER00822

Names & Taxonomyi

Protein namesi
Recommended name:
Pteridine reductase 1 (EC:1.5.1.33)
Alternative name(s):
H region methotrexate resistance protein
Gene namesi
Name:PTR1
Synonyms:HMTXR
ORF Names:L1063.01, LmjF23.0270, LmjF_23_0270
OrganismiLeishmania major
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
Proteomesi
  • UP000000542 Componenti: Chromosome 23

Organism-specific databases

EuPathDBiTriTrypDB:LmjF.23.0270

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL6194
DrugBankiDB03461 2'-Monophosphoadenosine 5'-Diphosphoribose
DB02338 Nadph Dihydro-Nicotinamide-Adenine-Dinucleotidephosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000547531 – 288Pteridine reductase 1Add BLAST288

Interactioni

Subunit structurei

Homotetramer.2 Publications

Protein-protein interaction databases

STRINGi5664.LmjF.23.0270

Chemistry databases

BindingDBiQ01782

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 11Combined sources4
Helixi17 – 28Combined sources12
Beta strandi32 – 39Combined sources8
Helixi41 – 54Combined sources14
Beta strandi59 – 63Combined sources5
Beta strandi67 – 69Combined sources3
Beta strandi75 – 77Combined sources3
Helixi85 – 100Combined sources16
Beta strandi105 – 108Combined sources4
Helixi134 – 147Combined sources14
Helixi149 – 163Combined sources15
Helixi167 – 169Combined sources3
Beta strandi174 – 179Combined sources6
Turni182 – 185Combined sources4
Helixi192 – 212Combined sources21
Helixi213 – 215Combined sources3
Beta strandi217 – 227Combined sources11
Helixi230 – 232Combined sources3
Helixi235 – 242Combined sources8
Turni246 – 249Combined sources4
Helixi254 – 265Combined sources12
Helixi267 – 269Combined sources3
Beta strandi276 – 280Combined sources5
Helixi283 – 285Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E7WX-ray1.75A/B1-288[»]
1E92X-ray2.20A/B/C/D1-288[»]
1W0CX-ray2.60A/B/C/D/E/F/G/H1-288[»]
2BF7X-ray2.40A/B/C/D1-288[»]
2BFAX-ray2.70A/B/C/D1-288[»]
2BFMX-ray2.60A/B/C/D1-288[»]
2BFOX-ray2.60A/B/C/D1-288[»]
2BFPX-ray2.55A/B/C/D1-288[»]
2QHXX-ray2.61A/B/C/D1-288[»]
3H4VX-ray2.40A/B/C/D/E/F/G/H1-288[»]
5L42X-ray2.10A/B/C/D1-288[»]
5L4NX-ray2.35A/B/C/D1-288[»]
ProteinModelPortaliQ01782
SMRiQ01782
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01782

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0725 Eukaryota
COG1028 LUCA
InParanoidiQ01782
KOiK03793

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR014058 Pteridine_reductase
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR02685 pter_reduc_Leis, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequencei

Sequence statusi: Complete.

Q01782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPTVPVAL VTGAAKRLGR SIAEGLHAEG YAVCLHYHRS AAEANALSAT
60 70 80 90 100
LNARRPNSAI TVQADLSNVA TAPVSGADGS APVTLFTRCA ELVAACYTHW
110 120 130 140 150
GRCDVLVNNA SSFYPTPLLR NDEDGHEPCV GDREAMETAT ADLFGSNAIA
160 170 180 190 200
PYFLIKAFAH RFAGTPAKHR GTNYSIINMV DAMTNQPLLG YTIYTMAKGA
210 220 230 240 250
LEGLTRSAAL ELAPLQIRVN GVGPGLSVLV DDMPPAVWEG HRSKVPLYQR
260 270 280
DSSAAEVSDV VIFLCSSKAK YITGTCVKVD GGYSLTRA
Length:288
Mass (Da):30,457
Last modified:May 27, 2002 - v2
Checksum:iB8F6FC23018367E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162F → V in CAJ03998 (PubMed:16020728).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01699 Genomic DNA Translation: AAA29249.2
FR796419 Genomic DNA Translation: CAJ03998.1
PIRiA45168
RefSeqiXP_001683351.1, XM_001683299.1

Genome annotation databases

GeneDBiLmjF.23.0270:pep
GeneIDi5651987
KEGGilma:LMJF_23_0270

Similar proteinsi

Entry informationi

Entry nameiPTR1_LEIMA
AccessioniPrimary (citable) accession number: Q01782
Secondary accession number(s): Q4QBE7, Q9U1F8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 27, 2002
Last modified: April 25, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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