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Protein

Pteridine reductase 1

Gene

PTR1

Organism
Leishmania major
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).1 Publication

Catalytic activityi

5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH.

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from biopterin.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Pteridine reductase 1 (PTR1)
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from biopterin, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei175 – 1751Substrate1 Publication
Active sitei194 – 1941Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 4024NADPBy similarityAdd
BLAST

GO - Molecular functioni

  • pteridine reductase activity Source: GeneDB
  • thymidylate synthase activity Source: GeneDB

GO - Biological processi

  • cellular response to oxidative stress Source: GeneDB
  • pteridine metabolic process Source: GeneDB
  • response to methotrexate Source: UniProtKB-KW
  • tetrahydrobiopterin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Methotrexate resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.5.1.33. 2950.
UniPathwayiUPA00849; UER00822.

Names & Taxonomyi

Protein namesi
Recommended name:
Pteridine reductase 1 (EC:1.5.1.33)
Alternative name(s):
H region methotrexate resistance protein
Gene namesi
Name:PTR1
Synonyms:HMTXR
ORF Names:L1063.01, LmjF23.0270, LmjF_23_0270
OrganismiLeishmania major
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
Proteomesi
  • UP000000542 Componenti: Chromosome 23

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL6194.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Pteridine reductase 1PRO_0000054753Add
BLAST

Interactioni

Subunit structurei

Homotetramer.2 Publications

Protein-protein interaction databases

STRINGi5664.LmjF.23.0270.

Chemistry

BindingDBiQ01782.

Structurei

Secondary structure

1
288
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 114Combined sources
Helixi17 – 2812Combined sources
Beta strandi32 – 398Combined sources
Helixi41 – 5414Combined sources
Beta strandi59 – 635Combined sources
Beta strandi67 – 693Combined sources
Beta strandi75 – 773Combined sources
Helixi85 – 10016Combined sources
Beta strandi105 – 1084Combined sources
Helixi134 – 14714Combined sources
Helixi149 – 16315Combined sources
Helixi167 – 1693Combined sources
Beta strandi174 – 1796Combined sources
Turni182 – 1854Combined sources
Helixi192 – 21221Combined sources
Helixi213 – 2153Combined sources
Beta strandi217 – 22711Combined sources
Helixi230 – 2323Combined sources
Helixi235 – 2428Combined sources
Turni246 – 2494Combined sources
Helixi254 – 26512Combined sources
Helixi267 – 2693Combined sources
Beta strandi276 – 2805Combined sources
Helixi283 – 2853Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E7WX-ray1.75A/B1-288[»]
1E92X-ray2.20A/B/C/D1-288[»]
1W0CX-ray2.60A/B/C/D/E/F/G/H1-288[»]
2BF7X-ray2.40A/B/C/D1-288[»]
2BFAX-ray2.70A/B/C/D1-288[»]
2BFMX-ray2.60A/B/C/D1-288[»]
2BFOX-ray2.60A/B/C/D1-288[»]
2BFPX-ray2.55A/B/C/D1-288[»]
2QHXX-ray2.61A/B/C/D1-288[»]
3H4VX-ray2.40A/B/C/D/E/F/G/H1-288[»]
ProteinModelPortaliQ01782.
SMRiQ01782. Positions 6-288.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01782.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
InParanoidiQ01782.
KOiK03793.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR014058. Pteridine_reductase.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02685. pter_reduc_Leis. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPTVPVAL VTGAAKRLGR SIAEGLHAEG YAVCLHYHRS AAEANALSAT
60 70 80 90 100
LNARRPNSAI TVQADLSNVA TAPVSGADGS APVTLFTRCA ELVAACYTHW
110 120 130 140 150
GRCDVLVNNA SSFYPTPLLR NDEDGHEPCV GDREAMETAT ADLFGSNAIA
160 170 180 190 200
PYFLIKAFAH RFAGTPAKHR GTNYSIINMV DAMTNQPLLG YTIYTMAKGA
210 220 230 240 250
LEGLTRSAAL ELAPLQIRVN GVGPGLSVLV DDMPPAVWEG HRSKVPLYQR
260 270 280
DSSAAEVSDV VIFLCSSKAK YITGTCVKVD GGYSLTRA
Length:288
Mass (Da):30,457
Last modified:May 27, 2002 - v2
Checksum:iB8F6FC23018367E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621F → V in CAJ03998 (PubMed:16020728).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01699 Genomic DNA. Translation: AAA29249.2.
FR796419 Genomic DNA. Translation: CAJ03998.1.
PIRiA45168.
RefSeqiXP_001683351.1. XM_001683299.1.

Genome annotation databases

GeneIDi5651987.
KEGGilma:LMJF_23_0270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01699 Genomic DNA. Translation: AAA29249.2.
FR796419 Genomic DNA. Translation: CAJ03998.1.
PIRiA45168.
RefSeqiXP_001683351.1. XM_001683299.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E7WX-ray1.75A/B1-288[»]
1E92X-ray2.20A/B/C/D1-288[»]
1W0CX-ray2.60A/B/C/D/E/F/G/H1-288[»]
2BF7X-ray2.40A/B/C/D1-288[»]
2BFAX-ray2.70A/B/C/D1-288[»]
2BFMX-ray2.60A/B/C/D1-288[»]
2BFOX-ray2.60A/B/C/D1-288[»]
2BFPX-ray2.55A/B/C/D1-288[»]
2QHXX-ray2.61A/B/C/D1-288[»]
3H4VX-ray2.40A/B/C/D/E/F/G/H1-288[»]
ProteinModelPortaliQ01782.
SMRiQ01782. Positions 6-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5664.LmjF.23.0270.

Chemistry

BindingDBiQ01782.
ChEMBLiCHEMBL6194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5651987.
KEGGilma:LMJF_23_0270.

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
InParanoidiQ01782.
KOiK03793.

Enzyme and pathway databases

UniPathwayiUPA00849; UER00822.
BRENDAi1.5.1.33. 2950.

Miscellaneous databases

EvolutionaryTraceiQ01782.
PROiQ01782.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR014058. Pteridine_reductase.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02685. pter_reduc_Leis. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTR1_LEIMA
AccessioniPrimary (citable) accession number: Q01782
Secondary accession number(s): Q4QBE7, Q9U1F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 27, 2002
Last modified: July 6, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.