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Protein

Pteridine reductase 1

Gene

PTR1

Organism
Leishmania major
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).1 Publication

Catalytic activityi

5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH.

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from biopterin.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Pteridine reductase 1 (PTR1)
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from biopterin, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei175Substrate1 Publication1
Active sitei194Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 40NADPBy similarityAdd BLAST24

GO - Molecular functioni

  • pteridine reductase activity Source: GeneDB
  • thymidylate synthase activity Source: GeneDB

GO - Biological processi

  • cellular response to oxidative stress Source: GeneDB
  • pteridine metabolic process Source: GeneDB
  • response to methotrexate Source: UniProtKB-KW
  • tetrahydrobiopterin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Methotrexate resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.5.1.33. 2950.
UniPathwayiUPA00849; UER00822.

Names & Taxonomyi

Protein namesi
Recommended name:
Pteridine reductase 1 (EC:1.5.1.33)
Alternative name(s):
H region methotrexate resistance protein
Gene namesi
Name:PTR1
Synonyms:HMTXR
ORF Names:L1063.01, LmjF23.0270, LmjF_23_0270
OrganismiLeishmania major
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
Proteomesi
  • UP000000542 Componenti: Chromosome 23

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL6194.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000547531 – 288Pteridine reductase 1Add BLAST288

Interactioni

Subunit structurei

Homotetramer.2 Publications

Protein-protein interaction databases

STRINGi5664.LmjF.23.0270.

Chemistry databases

BindingDBiQ01782.

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 11Combined sources4
Helixi17 – 28Combined sources12
Beta strandi32 – 39Combined sources8
Helixi41 – 54Combined sources14
Beta strandi59 – 63Combined sources5
Beta strandi67 – 69Combined sources3
Beta strandi75 – 77Combined sources3
Helixi85 – 100Combined sources16
Beta strandi105 – 108Combined sources4
Helixi134 – 147Combined sources14
Helixi149 – 163Combined sources15
Helixi167 – 169Combined sources3
Beta strandi174 – 179Combined sources6
Turni182 – 185Combined sources4
Helixi192 – 212Combined sources21
Helixi213 – 215Combined sources3
Beta strandi217 – 227Combined sources11
Helixi230 – 232Combined sources3
Helixi235 – 242Combined sources8
Turni246 – 249Combined sources4
Helixi254 – 265Combined sources12
Helixi267 – 269Combined sources3
Beta strandi276 – 280Combined sources5
Helixi283 – 285Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E7WX-ray1.75A/B1-288[»]
1E92X-ray2.20A/B/C/D1-288[»]
1W0CX-ray2.60A/B/C/D/E/F/G/H1-288[»]
2BF7X-ray2.40A/B/C/D1-288[»]
2BFAX-ray2.70A/B/C/D1-288[»]
2BFMX-ray2.60A/B/C/D1-288[»]
2BFOX-ray2.60A/B/C/D1-288[»]
2BFPX-ray2.55A/B/C/D1-288[»]
2QHXX-ray2.61A/B/C/D1-288[»]
3H4VX-ray2.40A/B/C/D/E/F/G/H1-288[»]
ProteinModelPortaliQ01782.
SMRiQ01782.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01782.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
InParanoidiQ01782.
KOiK03793.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR014058. Pteridine_reductase.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02685. pter_reduc_Leis. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPTVPVAL VTGAAKRLGR SIAEGLHAEG YAVCLHYHRS AAEANALSAT
60 70 80 90 100
LNARRPNSAI TVQADLSNVA TAPVSGADGS APVTLFTRCA ELVAACYTHW
110 120 130 140 150
GRCDVLVNNA SSFYPTPLLR NDEDGHEPCV GDREAMETAT ADLFGSNAIA
160 170 180 190 200
PYFLIKAFAH RFAGTPAKHR GTNYSIINMV DAMTNQPLLG YTIYTMAKGA
210 220 230 240 250
LEGLTRSAAL ELAPLQIRVN GVGPGLSVLV DDMPPAVWEG HRSKVPLYQR
260 270 280
DSSAAEVSDV VIFLCSSKAK YITGTCVKVD GGYSLTRA
Length:288
Mass (Da):30,457
Last modified:May 27, 2002 - v2
Checksum:iB8F6FC23018367E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162F → V in CAJ03998 (PubMed:16020728).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01699 Genomic DNA. Translation: AAA29249.2.
FR796419 Genomic DNA. Translation: CAJ03998.1.
PIRiA45168.
RefSeqiXP_001683351.1. XM_001683299.1.

Genome annotation databases

GeneIDi5651987.
KEGGilma:LMJF_23_0270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01699 Genomic DNA. Translation: AAA29249.2.
FR796419 Genomic DNA. Translation: CAJ03998.1.
PIRiA45168.
RefSeqiXP_001683351.1. XM_001683299.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E7WX-ray1.75A/B1-288[»]
1E92X-ray2.20A/B/C/D1-288[»]
1W0CX-ray2.60A/B/C/D/E/F/G/H1-288[»]
2BF7X-ray2.40A/B/C/D1-288[»]
2BFAX-ray2.70A/B/C/D1-288[»]
2BFMX-ray2.60A/B/C/D1-288[»]
2BFOX-ray2.60A/B/C/D1-288[»]
2BFPX-ray2.55A/B/C/D1-288[»]
2QHXX-ray2.61A/B/C/D1-288[»]
3H4VX-ray2.40A/B/C/D/E/F/G/H1-288[»]
ProteinModelPortaliQ01782.
SMRiQ01782.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5664.LmjF.23.0270.

Chemistry databases

BindingDBiQ01782.
ChEMBLiCHEMBL6194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5651987.
KEGGilma:LMJF_23_0270.

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
InParanoidiQ01782.
KOiK03793.

Enzyme and pathway databases

UniPathwayiUPA00849; UER00822.
BRENDAi1.5.1.33. 2950.

Miscellaneous databases

EvolutionaryTraceiQ01782.
PROiQ01782.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR014058. Pteridine_reductase.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02685. pter_reduc_Leis. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTR1_LEIMA
AccessioniPrimary (citable) accession number: Q01782
Secondary accession number(s): Q4QBE7, Q9U1F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 27, 2002
Last modified: November 2, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.