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Q01771

- STADS_BRANA

UniProt

Q01771 - STADS_BRANA

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Protein

Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplastic

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta(10) of the acyl chain.

Catalytic activityi

Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O.

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi141 – 1411Iron 1By similarity
Metal bindingi179 – 1791Iron 1By similarity
Metal bindingi179 – 1791Iron 2By similarity
Metal bindingi182 – 1821Iron 1; via pros nitrogenBy similarity
Metal bindingi232 – 2321Iron 2By similarity
Metal bindingi265 – 2651Iron 1By similarity
Metal bindingi265 – 2651Iron 2By similarity
Metal bindingi268 – 2681Iron 2; via pros nitrogenBy similarity

GO - Molecular functioni

  1. acyl-[acyl-carrier-protein] desaturase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplastic (EC:1.14.19.2)
Alternative name(s):
Stearoyl-ACP desaturase
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Plastidchloroplast Curated

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434ChloroplastBy similarityAdd
BLAST
Chaini35 – 399365Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplasticPRO_0000007128Add
BLAST

Proteomic databases

PRIDEiQ01771.

Expressioni

Tissue specificityi

Developing seeds.

Developmental stagei

Induced by 25 days after anthesis (dAA), peaking at 45 dAA but decreasing considerably thereafter.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ01771.
SMRiQ01771. Positions 56-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fatty acid desaturase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR005803. Stearoyl-ACP_desaturase_CS.
[Graphical view]
PfamiPF03405. FA_desaturase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00574. FATTY_ACID_DESATUR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01771-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALKFNPLVS QPYKLASSAR PPVSTFRSPK FLCLASSSSP ALSSKEVESL
60 70 80 90 100
KKPFTPPREV HLQVLHSMPP QKIEIFKSME DRAEQNLLPH LKDVEKSWQP
110 120 130 140 150
QDFLPDPASD GFEDQVKELR ERARELPDDY FVVLVGDMIT EEALPTYQTM
160 170 180 190 200
LNTLDGVRDE TGASPTSWAV WTRAWTAEEN RHGDLLNKYL YLSGRVDMRQ
210 220 230 240 250
IEKTIQYLIG SGMDPRTENN PYLGFIYTSF QERATFVSHG NTARQAKEHG
260 270 280 290 300
DLKLAQICGT IAADEKRHET AYTKIVEKLL EIDPDGTVVA FADMMRKKIS
310 320 330 340 350
MPAHLMYDGR DDNLFDNFSS VAQRLGVYTA KDYADILEFL AGRWRIESLT
360 370 380 390
GLSGEGNKAQ EYLCGLTPRI RRLDERAQAR AKKGPKIPFS WIHDREVQL
Length:399
Mass (Da):45,359
Last modified:April 1, 1993 - v1
Checksum:i8537254C13D9AF80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63364 mRNA. Translation: CAA44964.1.
PIRiS24995.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63364 mRNA. Translation: CAA44964.1 .
PIRi S24995.

3D structure databases

ProteinModelPortali Q01771.
SMRi Q01771. Positions 56-399.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q01771.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00199 .

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR005803. Stearoyl-ACP_desaturase_CS.
[Graphical view ]
Pfami PF03405. FA_desaturase_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00574. FATTY_ACID_DESATUR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and temporal regulation of a seed-specific Brassica napus cDNA encoding a stearoyl-acyl carrier protein (ACP) desaturase."
    Slocombe S.P., Cummins I., Jarvis R.P., Murphy D.J.
    Plant Mol. Biol. 20:151-155(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Jet neuf.
    Tissue: Embryo.

Entry informationi

Entry nameiSTADS_BRANA
AccessioniPrimary (citable) accession number: Q01771
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 26, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3