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Q01771 (STADS_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplastic

EC=1.14.19.2
Alternative name(s):
Stearoyl-ACP desaturase
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta10 of the acyl chain.

Catalytic activity

Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Lipid metabolism; fatty acid metabolism.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastidchloroplast Probable.

Tissue specificity

Developing seeds.

Developmental stage

Induced by 25 days after anthesis (dAA), peaking at 45 dAA but decreasing considerably thereafter.

Sequence similarities

Belongs to the fatty acid desaturase family.

Ontologies

Keywords
   Biological processFatty acid biosynthesis
Fatty acid metabolism
Lipid biosynthesis
Lipid metabolism
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandIron
Metal-binding
NADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processfatty acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacyl-[acyl-carrier-protein] desaturase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3434Chloroplast By similarity
Chain35 – 399365Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplastic
PRO_0000007128

Sites

Metal binding1411Iron 1 By similarity
Metal binding1791Iron 1 By similarity
Metal binding1791Iron 2 By similarity
Metal binding1821Iron 1; via pros nitrogen By similarity
Metal binding2321Iron 2 By similarity
Metal binding2651Iron 1 By similarity
Metal binding2651Iron 2 By similarity
Metal binding2681Iron 2; via pros nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q01771 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 8537254C13D9AF80

FASTA39945,359
        10         20         30         40         50         60 
MALKFNPLVS QPYKLASSAR PPVSTFRSPK FLCLASSSSP ALSSKEVESL KKPFTPPREV 

        70         80         90        100        110        120 
HLQVLHSMPP QKIEIFKSME DRAEQNLLPH LKDVEKSWQP QDFLPDPASD GFEDQVKELR 

       130        140        150        160        170        180 
ERARELPDDY FVVLVGDMIT EEALPTYQTM LNTLDGVRDE TGASPTSWAV WTRAWTAEEN 

       190        200        210        220        230        240 
RHGDLLNKYL YLSGRVDMRQ IEKTIQYLIG SGMDPRTENN PYLGFIYTSF QERATFVSHG 

       250        260        270        280        290        300 
NTARQAKEHG DLKLAQICGT IAADEKRHET AYTKIVEKLL EIDPDGTVVA FADMMRKKIS 

       310        320        330        340        350        360 
MPAHLMYDGR DDNLFDNFSS VAQRLGVYTA KDYADILEFL AGRWRIESLT GLSGEGNKAQ 

       370        380        390 
EYLCGLTPRI RRLDERAQAR AKKGPKIPFS WIHDREVQL 

« Hide

References

[1]"Nucleotide sequence and temporal regulation of a seed-specific Brassica napus cDNA encoding a stearoyl-acyl carrier protein (ACP) desaturase."
Slocombe S.P., Cummins I., Jarvis R.P., Murphy D.J.
Plant Mol. Biol. 20:151-155(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Jet neuf.
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63364 mRNA. Translation: CAA44964.1.
PIRS24995.

3D structure databases

ProteinModelPortalQ01771.
SMRQ01771. Positions 56-399.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ01771.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00199.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR005803. Stearoyl-ACP_desaturase_CS.
[Graphical view]
PfamPF03405. FA_desaturase_2. 1 hit.
[Graphical view]
PIRSFPIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00574. FATTY_ACID_DESATUR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSTADS_BRANA
AccessionPrimary (citable) accession number: Q01771
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways