Q01771 (STADS_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplastic EC=1.14.19.2 Alternative name(s): Stearoyl-ACP desaturase
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta10 of the acyl chain.
Catalytic activity	Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Lipid metabolism; fatty acid metabolism.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast Probable.
Tissue specificity	Developing seeds.
Developmental stage	Induced by 25 days after anthesis (dAA), peaking at 45 dAA but decreasing considerably thereafter.
Sequence similarities	Belongs to the fatty acid desaturase family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acyl-[acyl-carrier-protein] desaturase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Chloroplast By similarity
	Chain	35 – 399	365	Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplastic		PRO_0000007128
Sites
	Metal binding	141	1	Iron 1 By similarity
	Metal binding	179	1	Iron 1 By similarity
	Metal binding	179	1	Iron 2 By similarity
	Metal binding	182	1	Iron 1; via pros nitrogen By similarity
	Metal binding	232	1	Iron 2 By similarity
	Metal binding	265	1	Iron 1 By similarity
	Metal binding	265	1	Iron 2 By similarity
	Metal binding	268	1	Iron 2; via pros nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01771 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 8537254C13D9AF80	FASTA	399	45,359
	10 20 30 40 50 60 MALKFNPLVS QPYKLASSAR PPVSTFRSPK FLCLASSSSP ALSSKEVESL KKPFTPPREV 70 80 90 100 110 120 HLQVLHSMPP QKIEIFKSME DRAEQNLLPH LKDVEKSWQP QDFLPDPASD GFEDQVKELR 130 140 150 160 170 180 ERARELPDDY FVVLVGDMIT EEALPTYQTM LNTLDGVRDE TGASPTSWAV WTRAWTAEEN 190 200 210 220 230 240 RHGDLLNKYL YLSGRVDMRQ IEKTIQYLIG SGMDPRTENN PYLGFIYTSF QERATFVSHG 250 260 270 280 290 300 NTARQAKEHG DLKLAQICGT IAADEKRHET AYTKIVEKLL EIDPDGTVVA FADMMRKKIS 310 320 330 340 350 360 MPAHLMYDGR DDNLFDNFSS VAQRLGVYTA KDYADILEFL AGRWRIESLT GLSGEGNKAQ 370 380 390 EYLCGLTPRI RRLDERAQAR AKKGPKIPFS WIHDREVQL

References

[1]

"Nucleotide sequence and temporal regulation of a seed-specific Brassica napus cDNA encoding a stearoyl-acyl carrier protein (ACP) desaturase." Slocombe S.P., Cummins I., Jarvis R.P., Murphy D.J. Plant Mol. Biol. 20:151-155(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Jet neuf. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63364 mRNA. Translation: CAA44964.1.
PIR	S24995.
3D structure databases
ProteinModelPortal	Q01771.
SMR	Q01771. Positions 56-399.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00199.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR005067. Fatty_acid_desaturase-2. IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR005803. Stearoyl-ACP_desaturase_CS. [Graphical view]
Pfam	PF03405. FA_desaturase_2. 1 hit. [Graphical view]
PIRSF	PIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00574. FATTY_ACID_DESATUR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STADS_BRANA

Accession

Primary (citable) accession number: Q01771

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program