Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q01771

- STADS_BRANA

UniProt

Q01771 - STADS_BRANA

Protein

Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplastic

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta(10) of the acyl chain.

    Catalytic activityi

    Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O.

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi141 – 1411Iron 1By similarity
    Metal bindingi179 – 1791Iron 1By similarity
    Metal bindingi179 – 1791Iron 2By similarity
    Metal bindingi182 – 1821Iron 1; via pros nitrogenBy similarity
    Metal bindingi232 – 2321Iron 2By similarity
    Metal bindingi265 – 2651Iron 1By similarity
    Metal bindingi265 – 2651Iron 2By similarity
    Metal bindingi268 – 2681Iron 2; via pros nitrogenBy similarity

    GO - Molecular functioni

    1. acyl-[acyl-carrier-protein] desaturase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    Iron, Metal-binding, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplastic (EC:1.14.19.2)
    Alternative name(s):
    Stearoyl-ACP desaturase
    OrganismiBrassica napus (Rape)
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434ChloroplastBy similarityAdd
    BLAST
    Chaini35 – 399365Acyl-[acyl-carrier-protein] desaturase, seed specific, chloroplasticPRO_0000007128Add
    BLAST

    Proteomic databases

    PRIDEiQ01771.

    Expressioni

    Tissue specificityi

    Developing seeds.

    Developmental stagei

    Induced by 25 days after anthesis (dAA), peaking at 45 dAA but decreasing considerably thereafter.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ01771.
    SMRiQ01771. Positions 56-399.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the fatty acid desaturase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR005067. Fatty_acid_desaturase-2.
    IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR005803. Stearoyl-ACP_desaturase_CS.
    [Graphical view]
    PfamiPF03405. FA_desaturase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000346. Dlt9_acylACP_des. 1 hit.
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00574. FATTY_ACID_DESATUR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01771-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKFNPLVS QPYKLASSAR PPVSTFRSPK FLCLASSSSP ALSSKEVESL    50
    KKPFTPPREV HLQVLHSMPP QKIEIFKSME DRAEQNLLPH LKDVEKSWQP 100
    QDFLPDPASD GFEDQVKELR ERARELPDDY FVVLVGDMIT EEALPTYQTM 150
    LNTLDGVRDE TGASPTSWAV WTRAWTAEEN RHGDLLNKYL YLSGRVDMRQ 200
    IEKTIQYLIG SGMDPRTENN PYLGFIYTSF QERATFVSHG NTARQAKEHG 250
    DLKLAQICGT IAADEKRHET AYTKIVEKLL EIDPDGTVVA FADMMRKKIS 300
    MPAHLMYDGR DDNLFDNFSS VAQRLGVYTA KDYADILEFL AGRWRIESLT 350
    GLSGEGNKAQ EYLCGLTPRI RRLDERAQAR AKKGPKIPFS WIHDREVQL 399
    Length:399
    Mass (Da):45,359
    Last modified:April 1, 1993 - v1
    Checksum:i8537254C13D9AF80
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63364 mRNA. Translation: CAA44964.1.
    PIRiS24995.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63364 mRNA. Translation: CAA44964.1 .
    PIRi S24995.

    3D structure databases

    ProteinModelPortali Q01771.
    SMRi Q01771. Positions 56-399.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q01771.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00199 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR005067. Fatty_acid_desaturase-2.
    IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR005803. Stearoyl-ACP_desaturase_CS.
    [Graphical view ]
    Pfami PF03405. FA_desaturase_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000346. Dlt9_acylACP_des. 1 hit.
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00574. FATTY_ACID_DESATUR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and temporal regulation of a seed-specific Brassica napus cDNA encoding a stearoyl-acyl carrier protein (ACP) desaturase."
      Slocombe S.P., Cummins I., Jarvis R.P., Murphy D.J.
      Plant Mol. Biol. 20:151-155(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Jet neuf.
      Tissue: Embryo.

    Entry informationi

    Entry nameiSTADS_BRANA
    AccessioniPrimary (citable) accession number: Q01771
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3