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Protein

Nucleoside diphosphate kinase B

Gene

Nme2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC. Exhibits histidine protein kinase activity (By similarity).By similarity1 Publication

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATPBy similarity
Binding sitei60 – 601ATPBy similarity
Binding sitei88 – 881ATPBy similarity
Binding sitei94 – 941ATPBy similarity
Binding sitei105 – 1051ATPBy similarity
Binding sitei115 – 1151ATPBy similarity
Active sitei118 – 1181Pros-phosphohistidine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase B (EC:2.7.4.6)
Short name:
NDK B
Short name:
NDP kinase B
Alternative name(s):
Histidine protein kinase NDKB (EC:2.7.13.3)
P18
nm23-M2
Gene namesi
Name:Nme2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97356. Nme2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Cell projectionlamellipodium By similarity
  • Cell projectionruffle By similarity

  • Note: Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.By similarity

GO - Cellular componenti

  • cell periphery Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: MGI
  • lamellipodium Source: UniProtKB
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
  • ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired activation of the K+ channel Kcnn4, resulting in defective T-cell activation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Nucleoside diphosphate kinase BPRO_0000137118Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ01768.
PaxDbiQ01768.
PRIDEiQ01768.

2D gel databases

REPRODUCTION-2DPAGEQ01768.
SWISS-2DPAGEQ01768.

PTM databases

iPTMnetiQ01768.
PhosphoSiteiQ01768.
SwissPalmiQ01768.

Expressioni

Tissue specificityi

Expressed in the base region of the oxyntic and pyloric mucosae.1 Publication

Gene expression databases

BgeeiQ01768.
ExpressionAtlasiQ01768. baseline and differential.
GenevisibleiQ01768. MM.

Interactioni

Subunit structurei

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with AKAP13. Interacts ITGB1BP1 (via C-terminal domain region) (By similarity). Interacts with CAPN8.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Itgb1P090553EBI-642573,EBI-644224

Protein-protein interaction databases

BioGridi201789. 2 interactions.
IntActiQ01768. 11 interactions.
MINTiMINT-1573552.
STRINGi10090.ENSMUSP00000021217.

Structurei

3D structure databases

ProteinModelPortaliQ01768.
SMRiQ01768. Positions 2-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6666Interaction with AKAP13By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiQ01768.
KOiK00940.
OrthoDBiEOG7GJ6FG.
PhylomeDBiQ01768.
TreeFamiTF106373.

Family and domain databases

HAMAPiMF_00451. NDP_kinase.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ
60 70 80 90 100
HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVESAEKEI HLWFKPEELI DYKSCAHDWV

YE
Length:152
Mass (Da):17,363
Last modified:April 1, 1993 - v1
Checksum:i1A5C3F84C1FFC83C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68193 mRNA. Translation: CAA48275.1.
AK012447 mRNA. Translation: BAB28246.1.
BC066995 mRNA. Translation: AAH66995.1.
BC086892 mRNA. Translation: AAH86892.1.
BC086893 mRNA. Translation: AAH86893.1.
CCDSiCCDS25246.1.
PIRiS29241.
RefSeqiNP_001070997.1. NM_001077529.2.
NP_032731.1. NM_008705.5.
UniGeneiMm.1260.

Genome annotation databases

EnsembliENSMUST00000021217; ENSMUSP00000021217; ENSMUSG00000020857.
ENSMUST00000072566; ENSMUSP00000103476; ENSMUSG00000020857.
GeneIDi18103.
KEGGimmu:18103.
UCSCiuc007kxs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68193 mRNA. Translation: CAA48275.1.
AK012447 mRNA. Translation: BAB28246.1.
BC066995 mRNA. Translation: AAH66995.1.
BC086892 mRNA. Translation: AAH86892.1.
BC086893 mRNA. Translation: AAH86893.1.
CCDSiCCDS25246.1.
PIRiS29241.
RefSeqiNP_001070997.1. NM_001077529.2.
NP_032731.1. NM_008705.5.
UniGeneiMm.1260.

3D structure databases

ProteinModelPortaliQ01768.
SMRiQ01768. Positions 2-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201789. 2 interactions.
IntActiQ01768. 11 interactions.
MINTiMINT-1573552.
STRINGi10090.ENSMUSP00000021217.

PTM databases

iPTMnetiQ01768.
PhosphoSiteiQ01768.
SwissPalmiQ01768.

2D gel databases

REPRODUCTION-2DPAGEQ01768.
SWISS-2DPAGEQ01768.

Proteomic databases

EPDiQ01768.
PaxDbiQ01768.
PRIDEiQ01768.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021217; ENSMUSP00000021217; ENSMUSG00000020857.
ENSMUST00000072566; ENSMUSP00000103476; ENSMUSG00000020857.
GeneIDi18103.
KEGGimmu:18103.
UCSCiuc007kxs.2. mouse.

Organism-specific databases

CTDi4831.
MGIiMGI:97356. Nme2.

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiQ01768.
KOiK00940.
OrthoDBiEOG7GJ6FG.
PhylomeDBiQ01768.
TreeFamiTF106373.

Miscellaneous databases

PROiQ01768.
SOURCEiSearch...

Gene expression databases

BgeeiQ01768.
ExpressionAtlasiQ01768. baseline and differential.
GenevisibleiQ01768. MM.

Family and domain databases

HAMAPiMF_00451. NDP_kinase.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional expression of the second mouse nm23/NDP kinase gene, nm23-M2."
    Urano T., Takamiya K., Furukawa K., Shiku H.
    FEBS Lett. 309:358-362(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. Takeshi U.
    Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Heart.
  5. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 7-26; 57-66 AND 89-143, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain and Hippocampus.
  6. "Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP."
    Hata S., Koyama S., Kawahara H., Doi N., Maeda T., Toyama-Sorimachi N., Abe K., Suzuki K., Sorimachi H.
    J. Biol. Chem. 281:11214-11224(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CAPN8, TISSUE SPECIFICITY.
  7. "Nucleoside diphosphate kinase B knock-out mice have impaired activation of the K+ channel KCa3.1, resulting in defective T cell activation."
    Di L., Srivastava S., Zhdanova O., Sun Y., Li Z., Skolnik E.Y.
    J. Biol. Chem. 285:38765-38771(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiNDKB_MOUSE
AccessioniPrimary (citable) accession number: Q01768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 8, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.