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Protein

Nucleoside diphosphate kinase B

Gene

Nme2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC. Exhibits histidine protein kinase activity (By similarity).By similarity1 Publication

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATPBy similarity1
Binding sitei60ATPBy similarity1
Binding sitei88ATPBy similarity1
Binding sitei94ATPBy similarity1
Binding sitei105ATPBy similarity1
Binding sitei115ATPBy similarity1
Active sitei118Pros-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-499943. Interconversion of nucleotide di- and triphosphates.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase B (EC:2.7.4.6)
Short name:
NDK B
Short name:
NDP kinase B
Alternative name(s):
Histidine protein kinase NDKB (EC:2.7.13.3)
P18
nm23-M2
Gene namesi
Name:Nme2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97356. Nme2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired activation of the K+ channel Kcnn4, resulting in defective T-cell activation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001371181 – 152Nucleoside diphosphate kinase BAdd BLAST152

Proteomic databases

EPDiQ01768.
PaxDbiQ01768.
PeptideAtlasiQ01768.
PRIDEiQ01768.

2D gel databases

REPRODUCTION-2DPAGEiQ01768.
SWISS-2DPAGEiQ01768.

PTM databases

iPTMnetiQ01768.
PhosphoSitePlusiQ01768.
SwissPalmiQ01768.

Expressioni

Tissue specificityi

Expressed in the base region of the oxyntic and pyloric mucosae.1 Publication

Gene expression databases

BgeeiENSMUSG00000020857.
ExpressionAtlasiQ01768. baseline and differential.
GenevisibleiQ01768. MM.

Interactioni

Subunit structurei

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with AKAP13. Interacts ITGB1BP1 (via C-terminal domain region) (By similarity). Interacts with CAPN8.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Itgb1P090553EBI-642573,EBI-644224

Protein-protein interaction databases

BioGridi201789. 2 interactors.
IntActiQ01768. 11 interactors.
MINTiMINT-1573552.
STRINGi10090.ENSMUSP00000021217.

Structurei

3D structure databases

ProteinModelPortaliQ01768.
SMRiQ01768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 66Interaction with AKAP13By similarityAdd BLAST66

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiQ01768.
KOiK00940.
OrthoDBiEOG091G0PLD.
PhylomeDBiQ01768.
TreeFamiTF106373.

Family and domain databases

Gene3Di3.30.70.141. 1 hit.
HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiView protein in InterPro
IPR034907. NDK-like_dom.
IPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
PfamiView protein in Pfam
PF00334. NDK. 1 hit.
PRINTSiPR01243. NUCDPKINASE.
SMARTiView protein in SMART
SM00562. NDK. 1 hit.
SUPFAMiSSF54919. SSF54919. 1 hit.
PROSITEiView protein in PROSITE
PS00469. NDP_KINASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ
60 70 80 90 100
HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVESAEKEI HLWFKPEELI DYKSCAHDWV

YE
Length:152
Mass (Da):17,363
Last modified:April 1, 1993 - v1
Checksum:i1A5C3F84C1FFC83C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68193 mRNA. Translation: CAA48275.1.
AK012447 mRNA. Translation: BAB28246.1.
BC066995 mRNA. Translation: AAH66995.1.
BC086892 mRNA. Translation: AAH86892.1.
BC086893 mRNA. Translation: AAH86893.1.
CCDSiCCDS25246.1.
PIRiS29241.
RefSeqiNP_001070997.1. NM_001077529.2.
NP_032731.1. NM_008705.5.
UniGeneiMm.1260.

Genome annotation databases

EnsembliENSMUST00000021217; ENSMUSP00000021217; ENSMUSG00000020857.
ENSMUST00000072566; ENSMUSP00000103476; ENSMUSG00000020857.
GeneIDi18103.
KEGGimmu:18103.
UCSCiuc007kxs.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiNDKB_MOUSE
AccessioniPrimary (citable) accession number: Q01768
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 27, 2017
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families